Xylose Isomerases and Their Uses

ABSTRACT

This disclosure relates to novel xylose isomerases and their uses, particularly in fermentation processes that employ xylose-containing media.

This application claims the benefit under 35 U.S.C. §119(e) of U.S.provisional application No. 61/675,241, filed Jul. 24, 2012, thecontents of which are incorporated by reference in their entiretiesherein.

1. BACKGROUND

The efficient, commercial production of biofuels from plant material,such as sugarcane, requires the fermentation of pentoses, such asxylose. Xylose in plant material typically comes from lignocellulose,which is a matrix composed of cellulose, hemicelluloses, and lignin.Lignocellulose is broken down either by acid hydrolysis or enzymaticreaction, yielding xylose in addition to other monosaccharides, such asglucose (Maki et al., 2009, Int. J. Biol. Sci. 5:500-516).

Fungi, especially Saccharomyces cerevisiae, are commercially relevantmicroorganisms that ferment sugars into biofuels such as ethanol.However, S. cerevisiae does not endogenously metabolize xylose,requiring genetic modifications that allow it to convert xylose intoxylulose. Other organisms, whose usefulness in ethanol production islimited, are able to metabolize xylose (Nevigot, 2008, Micobiol. Mol.Biol. Rev. 72:379-412).

Two pathways have been identified for the metabolism of xylose toxylulose in microorganisms: the xylose reductase (XR, EC1.1.1.307)/xylitol dehydrogenase (XDH, EC 1.1.1.9, 1.1.1.10 and1.1.1.B19) pathway and the xylose isomerase (XI, EC 5.3.1.5) pathway.Use of the XR/XDH pathway for xylose metabolism creates an imbalance ofcofactors (excess NADH and NADP+) limiting the potential output of thispathway for the production of ethanol. The XI pathway, on the otherhand,converts xylose to xylulose in a single step and does not create acofactor imbalance (Young et al., 2010, Biotechnol. Biofuels 3:24-36).

Because S. cerevisiae does not possess a native XI, it has beendesirable to search for an XI in another organism to insert into S.cerevisiae for the purpose of biofuels production. Several XI genes havebeen discovered, although little or no enzymatic activity uponexpression in S. cerevisiae has been a common problem. The XI fromPiromyces sp. E2 was the first heterologously expressed XI in S.cerevisiae whose enzymatic activity could be observed (WO 03/062430).

2. SUMMARY

Due to the physiology of S. cerevisiae and the process of commercialbiofuel production, there are other characteristics besides activitythat are valuable in a commercially useful XI. During fermentation, thepH of the yeast cell and its environment can become more acidic (Rosaand Sa-Correia, 1991, Appl. Environ. Microbiol. 57:830-835). The abilityof the XI to function in an acidic environment is therefore highlydesirable. Therefore, there is a still a need in the art for XI enzymeswith enhanced activity to convert xylose to xylulose for biofuelsproduction under a broader range of commercially relevant conditions.

The present disclosure relates to novel xylose isomerases. The xyloseisomerases have desirable characteristics for xylose fermentation, suchas high activity, tolerance to acidic conditions (i.e., pH levels below7, e.g., pH 6.5 or pH 6), or both.

The present disclosure has multiple aspects. In one aspect, thedisclosure is directed to XI polypeptides. The polypeptides of thedisclosure typically comprise amino acid sequences having at least 70%,75%, 80%, 85%, 90%, 93%, 95%, 96%, 98%, 99% or 100% sequence identity toany of the XI polypeptides of Table 1, or the catalytic domain ordimerization domain thereof, or are encoded by nucleic acid sequencescomprising nucleotide sequences having at least 70%, 75%, 80%, 85%, 90%,93%, 95%, 96%, 98%, 99% or 100% sequence identity to any of the nucleicacids of Table 1:

TABLE 1 SEQ Organism Type of Catalytic Dimerization ID NO: Clone No.Classification Sequence Domain Domain 1 1754MI2_001 Bacteroidales DNA 21754MI2_001 Bacteroidales Amino Acid 2-376 377-437 3 5586MI6_004Bacteroidales DNA 4 5586MI6_004 Bacteroidales Amino Acid 2-376 377-437 55749MI1_003 Bacteroidales DNA 6 5749MI1_003 Bacteroidales Amino Acid2-381 382-442 7 5750MI1_003 Bacteroidales DNA 8 5750MI1_003Bacteroidales Amino Acid 2-381 382-442 9 5750MI2_003 Bacteroidales DNA10 5750MI2_003 Bacteroidales Amino Acid 2-381 382-442 11 5586MI5_004Bacteroides DNA 12 5586MI5_004 Bacteroides Amino Acid 2-375 376-435 135586MI202_004 Bacteroides DNA 14 5586MI202_004 Bacteroides Amino Acid2-377 378-438 15 5586MI211_003 Bacteroides DNA 16 5586MI211_003Bacteroides Amino Acid 2-376 377-437 17 5606MI1_005 Bacteroides DNA 185606MI1_005 Bacteroides Amino Acid 2-377 378-438 19 5606MI2_003Bacteroides DNA 20 5606MI2_003 Bacteroides Amino Acid 2-378 379-439 215610MI3_003 Bacteroides DNA 22 5610MI3_003 Bacteroides Amino Acid 2-377378-439 23 5749MI2_004 Bacteroides DNA 24 5749MI2_004 Bacteroides AminoAcid 2-377 378-438 25 5750MI3_003 Bacteroides DNA 26 5750MI3_003Bacteroides Amino Acid 2-377 378-438 27 5750MI4_003 Bacteroides DNA 285750MI4_003 Bacteroides Amino Acid 2-377 378-438 29 5751MI4_002Bacteroides DNA 30 5751MI4_002 Bacteroides Amino Acid 2-376 377-437 315751MI5_003 Bacteroides DNA 32 5751MI5_003 Bacteroides Amino Acid 2-377378-438 33 5751MI6_004 Bacteroides DNA 34 5751MI6_004 Bacteroides AminoAcid 2-377 378-438 35 5586MI22_003 Clostridiales DNA 36 5586MI22_003Clostridiales Amino Acid 2-375 376-439 37 1753MI4_001 Firmicutes DNA 381753MI4_001 Firmicutes Amino Acid 2-374 375-440 39 1753MI6_001Firmicutes DNA 40 1753MI6_001 Firmicutes Amino Acid 2-374 375-440 411753MI35_004 Firmicutes DNA 42 1753MI35_004 Firmicutes Amino Acid 2-375376-441 43 1754MI9_004 Firmicutes DNA 44 1754MI9_004 Firmicutes AminoAcid 2-375 376-440 45 1754MI22_004 Firmicutes DNA 46 1754MI22_004Firmicutes Amino Acid 2-375 376-440 47 727MI1_002 Firmicutes DNA 48727MI1_002 Firmicutes Amino Acid 2-372 373-436 49 727MI9_005 FirmicutesDNA 50 727MI9_005 Firmicutes Amino Acid 2-374 375-438 51 727MI27_002Firmicutes DNA 52 727MI27_002 Firmicutes Amino Acid 2-374 375-439 531753MI2_006 Neocallimastigales DNA 54 1753MI2_006 NeocallimastigalesAmino Acid 2-376 377-437 55 5586MI3_005 Neocallimastigales DNA 565586MI3_005 Neocallimastigales Amino Acid 2-376 377-437 57 5586MI91_002Neocallimastigales DNA 58 5586MI91_002 Neocallimastigales Amino Acid2-376 377-437 59 5586MI194_003 Neocallimastigales DNA 60 5586MI194_003Neocallimastigales Amino Acid 2-376 377-438 61 5586MI198_003Neocallimastigales DNA 62 5586MI198_003 Neocallimastigales Amino Acid2-375 376-437 63 5586MI201_003 Neocallimastigales DNA 64 5586MI201_003Neocallimastigales Amino Acid 2-376 377-438 65 5586MI204_002Neocallimastigales DNA 66 5586MI204_002 Neocallimastigales Amino Acid2-375 376-437 67 5586MI207_002 Neocallimastigales DNA 68 5586MI207_002Neocallimastigales Amino Acid 2-375 376-437 69 5586MI209_003Neocallimastigales DNA 70 5586MI209_003 Neocallimastigales Amino Acid2-375 376-437 71 5586MI214_002 Neocallimastigales DNA 72 5586MI214_002Neocallimastigales Amino Acid 2-375 376-437 73 5751MI3_001Neocallimastigales DNA 74 5751MI3_001 Neocallimastigales Amino Acid2-375 376-437 75 5753MI3_002 Prevotella DNA 76 5753MI3_002 PrevotellaAmino Acid 2-376 377-439 77 1754MI1_001 Prevotella DNA 78 1754MI1_001Prevotella Amino Acid 2-377 378-439 79 1754MI3_007 Prevotella DNA 801754MI3_007 Prevotella Amino Acid 2-377 378-439 81 1754MI5_009Prevotella DNA 82 1754MI5_009 Prevotella Amino Acid 2-375 376-437 835586MI1_003 Prevotella DNA 84 5586MI1_003 Prevotella Amino Acid 2-377378-439 85 5586MI2_006 Prevotella DNA 86 5586MI2_006 Prevotella AminoAcid 2-377 378-439 87 5586MI8_003 Prevotella DNA 88 5586MI8_003Prevotella Amino Acid 2-377 378-439 89 5586MI14_003 Prevotella DNA 905586MI14_003 Prevotella Amino Acid 2-377 378-439 91 5586MI26_003Prevotella DNA 92 5586MI26_003 Prevotella Amino Acid 2-377 378-439 935586MI86_001 Prevotella DNA 94 5586MI86_001 Prevotella Amino Acid 2-376377-438 95 5586MI108_002 Prevotella DNA 96 5586MI108_002 PrevotellaAmino Acid 2-377 378-439 97 5586MI182_004 Prevotella DNA 985586MI182_004 Prevotella Amino Acid 2-377 378-439 99 5586MI193_004Prevotella DNA 100 5586MI193_004 Prevotella Amino Acid 2-376 377-438 1015586MI195_003 Prevotella DNA 102 5586MI195_003 Prevotella Amino Acid2-376 377-438 103 5586MI216_003 Prevotella DNA 104 5586MI216_003Prevotella Amino Acid 2-376 377-438 105 5586MI197_003 Prevotella DNA 1065586MI197_003 Prevotella Amino Acid 2-376 377-438 107 5586MI199_003Prevotella DNA 108 5586MI199_003 Prevotella Amino Acid 2-376 377-438 1095586MI200_003 Prevotella DNA 110 5586MI200_003 Prevotella Amino Acid2-376 377-438 111 5586MI203_003 Prevotella DNA 112 5586MI203_003Prevotella Amino Acid 2-376 377-438 113 5586MI205_004 Prevotella DNA 1145586MI205_004 Prevotella Amino Acid 2-376 377-438 115 5586MI206_004Prevotella DNA 116 5586MI206_004 Prevotella Amino Acid 2-376 377-438 1175586MI208_003 Prevotella DNA 118 5586MI208_003 Prevotella Amino Acid2-376 377-438 119 5586MI210_002 Prevotella DNA 120 5586MI210_002Prevotella Amino Acid 2-374 375-437 121 5586MI212_002 Prevotella DNA 1225586MI212_002 Prevotella Amino Acid 2-376 377-438 123 5586MI213_003Prevotella DNA 124 5586MI213_003 Prevotella Amino Acid 2-376 377-438 1255586MI215_003 Prevotella DNA 126 5586MI215_003 Prevotella Amino Acid2-376 377-438 127 5607MI1_003 Prevotella DNA 128 5607MI1_003 PrevotellaAmino Acid 2-376 377-438 129 5607MI2_003 Prevotella DNA 130 5607MI2_003Prevotella Amino Acid 2-376 377-442 131 5607MI3_003 Prevotella DNA 1325607MI3_003 Prevotella Amino Acid 2-376 377-438 133 5607MI4_005Prevotella DNA 134 5607MI4_005 Prevotella Amino Acid 2-376 377-438 1355607MI5_002 Prevotella DNA 136 5607MI5_002 Prevotella Amino Acid 2-376377-439 137 5607MI6_002 Prevotella DNA 138 5607MI6_002 Prevotella AminoAcid 2-376 377-438 139 5607MI7_002 Prevotella DNA 140 5607MI7_002Prevotella Amino Acid 2-376 377-438 141 5608MI1_004 Prevotella DNA 1425608MI1_004 Prevotella Amino Acid 2-376 377-438 143 5608MI2_002Prevotella DNA 144 5608MI2_002 Prevotella Amino Acid 2-375 376-437 1455608MI3_004 Prevotella DNA 146 5608MI3_004 Prevotella Amino Acid 2-376377-438 147 5609MI1_005 Prevotella DNA 148 5609MI1_005 Prevotella AminoAcid 2-376 377-438 149 5610MI1_003 Prevotella DNA 150 5610MI1_003Prevotella Amino Acid 2-376 377-438 151 5610MI2_004 Prevotella DNA 1525610MI2_004 Prevotella Amino Acid 2-376 377-438 153 5751MI1_003Prevotella DNA 154 5751MI1_003 Prevotella Amino Acid 2-376 377-438 1555751MI2_003 Prevotella DNA 156 5751MI2_003 Prevotella Amino Acid 2-376377-438 157 5752MI1_003 Prevotella DNA 158 5752MI1_003 Prevotella AminoAcid 2-376 377-438 159 5752MI2_003 Prevotella DNA 160 5752MI2_003Prevotella Amino Acid 2-376 377-438 161 5752MI3_002 Prevotella DNA 1625752MI3_002 Prevotella Amino Acid 2-376 377-438 163 5752MI5_003Prevotella DNA 164 5752MI5_003 Prevotella Amino Acid 2-376 377-438 1655752MI6_004 Prevotella DNA 166 5752MI6_004 Prevotella Amino Acid 2-376377-438 167 5753MI1_002 Prevotella DNA 168 5753MI1_002 Prevotella AminoAcid 2-376 377-438 169 5753MI2_002 Prevotella DNA 170 5753MI2_002Prevotella Amino Acid 2-376 377-438 171 5753MI4_002 Prevotella DNA 1725753MI4_002 Prevotella Amino Acid 2-376 377-438 173 5752MI4_004Prevotella DNA 174 5752MI4_004 Prevotella Amino Acid 2-376 377-438 175727MI4_006 Rhizobiales DNA 176 727MI4_006 Rhizobiales Amino Acid 2-373374-435

In specific embodiments, a polypeptide of the disclosure comprises anamino acid sequence having:

-   -   (1) (a) at least 97% or 98% sequence identity to SEQ ID NO:78 or        the catalytic domain thereof (amino acids 2-377 of SEQ ID NO:78)        and/or (b) at least 80%, 85%, 90%, 93% or 95% sequence identity        to SEQ ID NO:78 or the catalytic domain thereof (amino acids        2-377 of SEQ ID NO:78) and further comprises (i) SEQ ID NO:212        or SEQ ID NO:213 and/or (ii) SEQ ID NO:214;    -   (2) (a) at least 95%, 97% or 98% sequence identity to SEQ ID        NO:96 or the catalytic domain thereof (amino acids 2-377 of SEQ        ID NO:96) and/or (b) at least 80%, 85%, 90%, 93% or 95% sequence        identity to SEQ ID NO:96 or the catalytic domain thereof (amino        acids 2-377 of SEQ ID NO:96) and further comprises (i) SEQ ID        NO:212 or SEQ ID NO:213 and/or (ii) SEQ ID NO:214;    -   (3) at least 80%, 85%, 90%, 93%, 95%, 97% or 98% sequence        identity to SEQ ID NO:38 or the catalytic domain thereof (amino        acids 2-374 of SEQ ID NO:38), and optionally further comprises        one, two, three, four or all five of (i) SEQ ID NO:206 or SEQ ID        NO:207; (ii) SEQ ID NO:208; (iii) SEQ ID NO:209; (iv) SEQ ID        NO:210; and (iv) SEQ ID NO:211;    -   (4) at least 80%, 85%, 90%, 93%, 95%, 97% or 98% sequence        identity to SEQ ID NO:2 or the catalytic domain thereof (amino        acids 2-374 of SEQ ID NO:2);    -   (5) at least 93%, 95%, 97% or 98% sequence identity to SEQ ID        NO:58 or the catalytic domain thereof (amino acids 2-376 of SEQ        ID NO:58),    -   (6) at least 80%, 85%, 90%, 93%, 95%, 97% or 98% sequence        identity to SEQ ID NO:42 or the catalytic domain thereof (amino        acids 2-375 of SEQ ID NO:42), and optionally further comprises        one, two or all three of (i) SEQ ID NO:206 or SEQ ID        NO:207; (ii) SEQ ID NO:210; and (iii) SEQ ID NO:211;    -   (7) (a) at least 97% or 98% sequence identity to SEQ ID NO:84 or        the catalytic domain thereof (amino acids 2-376 of SEQ ID        NO:84), and/or (b) at least 80%, 85%, 90%, 93% or 95% sequence        identity to SEQ ID NO:84 or the catalytic domain thereof (amino        acids 2-376 of SEQ ID NO:84) and further comprises (i) SEQ ID        NO:212 or SEQ ID NO:213 and/or (ii) SEQ ID NO:214;    -   (8) (a) at least 97% or 98% sequence identity to SEQ ID NO:80 or        the catalytic domain thereof (amino acids 2-377 of SEQ ID NO:80)        and/or (b) at least 80%, 85%, 90%, 93% or 95% sequence identity        to SEQ ID NO:80 or the catalytic domain thereof (amino acids        2-377 of SEQ ID NO:80) and further comprises (i) SEQ ID NO:212        or SEQ ID NO:213 and/or (ii) SEQ ID NO:214;    -   (9) at least 93%, 95%, 97% or 98% sequence identity to SEQ ID        NO:54 or the catalytic domain thereof (amino acids 2-376 of SEQ        ID NO:54);    -   (10) at least 80%, 85%, 90%, 93%, 95%, 97% or 98% sequence        identity to SEQ ID NO:46 or the catalytic domain thereof (amino        acids 2-376 of SEQ ID NO:46), and optionally further comprises        SEQ ID NO:206 or SEQ ID NO:207;    -   (11) at least 90%, 93%, 95%, 97% or 98% sequence identity to SEQ        ID NO:16 or the catalytic domain thereof (amino acids 2-376 of        SEQ ID NO:16);    -   (12) at least 85%, 90%, 93%, 95%, 97% or 98% sequence identity        to SEQ ID NO:82 or the catalytic domain thereof (amino acids        2-375 of SEQ ID NO:82); and/or    -   (13) at least 90%, 93%, 95%, 97% or 98% sequence identity to SEQ        ID NO:32 or the catalytic domain thereof (amino acids 2-377 of        SEQ ID NO:32).

The XIs of the disclosure can be characterized in terms of theiractivity. In some embodiments, a XI of the disclosure has at least 1.3times the activity of the Orpinomyces sp. XI assigned Genbank AccessionNo. 169733248 (“Op-XI”) at pH 7.5, for example using the assay describedin any of Examples 4, 6 and 7. In certain specific embodiments, a XI ofthe disclosure has an activity ranging from 1.25 to 3.0 times, from 1.5to 3 times, from 1.5 to 2.25 times, or from 1.75 to 3 times the activityof Op-XI at pH 7.5.

The XIs of the disclosure can also be characterized in terms of theirtolerance to acidic environments (e.g., at a pH of 6.5 or 6). In someembodiments, a XI of the disclosure has at least 1.9 times the activityof the Op-XI at pH 6, for example using the assay described in Example7. In certain specific embodiments, a XI of the disclosure has anactivity ranging from 1.9 to 4.1 times, from 2.4 to 4.1 times, from 2.4to 3.9 times, or 2.4 to 4.1 times the activity of Op-XI at pH 6.

Tolerance to acidic environments can also be characterized as a ratio ofactivity at pH 6 to activity at pH 7.5 (“a pH 6 to pH 7.5 activityratio”), for example as measured using the assay of Example 7. In someembodiments, the pH 6 to pH 7.5 activity ratio is at least 0.5 or atleast 0.6. In various embodiments, the pH 6 to pH 7.5 activity ratio is0.5-0.9 or 0.6-0.9.

In another aspect, the disclosure is directed to a nucleic acid whichencodes a XI polypeptide of the disclosure. In various embodiments, thenucleic acid comprises a nucleotide sequence with at least 70%, 75%,80%, 85%, 90%, 93%, 95%, 96%, 98%, 99% or 100% sequence identity to thenucleotide sequence of any one of SEQ ID NOS:1, 3, 5, 7, 9, 11, 13, 15,17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51,53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87,89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117,119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145,147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173,and 175, or the portion of any of the foregoing sequences encoding a XIcatalytic domain or dimerization domain.

In other aspects, the disclosure is directed to a vector comprising aXI-encoding nucleotide sequence, for example a vector having an originof replication and/or a promoter sequence operably linked to theXI-encoding nucleotide sequence. The promoter sequence can be one thatis operable in a eukaryotic cell, for example in a fungal cell. In someembodiments, the promoter is operable in yeast (e.g., S. cerevisiae) orfilamentous fungi.

In yet another aspect, the disclosure is directed to a recombinant cellcomprising a nucleic acid that encodes a XI polypeptide. Particularly,the cell is engineered to express any of the XI polypeptides describedherein. The recombinant cell may be of any species, and is preferably aeukaryotic cell, for example a yeast cell. Suitable genera of yeastinclude Saccharomyces, Kluyveromyces, Candida, Pichia,Schizosaccharomyces, Hansenula, Klockera, Schwanniomyces, Issatchenkiaand Yarrowia. In specific embodiments, the recombinant cell is a S.cerevisiae, S. bulderi, S. barnetti, S. exiguus, S. uvarum, S.diastaticus, K. lactis, I. orientalis, K. marxianus or K. fragilis.Suitable genera of filamentous fungi include Aspergillus, Penicillium,Rhizopus, Chrysosporium, Myceliophthora, Trichoderma, Humicola,Acremonium and Fusarium. In specific embodiments, the recombinant cellis an Aspergillus niger, Aspergillus oryzae, Trichoderma reesei,Penicillium chrysogenum, Myceliophthora thermophila, or Rhizopus oryzae.

The recombinant cell may also be mutagenized or engineered to includemodifications other than the recombinant expression of XI, particularlythose that make the cell more suited to utilize xylose in a fermentationpathway. Exemplary additional modifications create one, two, three,four, five or even more of the following phenotypes: (a) increase inxylose transport into the cell; (b) increase in aerobic growth rate onxylose; (c) increase in xylulose kinase activity; (d) increase in fluxthrough the pentose phosphate pathway into glycolysis, (e) decrease inaldose reductase activity, (f) decrease in sensitivity to cataboliterepression, (g) increase in tolerance to biofuels, e.g., ethanol, (h)increase tolerance to intermediate production (e.g., xylitol), (i)increase in temperature tolerance, (j) osmolarity of organic acids, and(k) a reduced production of byproducts.

Increases in activity can be achieved by increased expression levels,for example expression of a hexose or pentose (e.g., xylose)transporter, a xylulose kinase, a glycolytic enzyme, or an ethanologenicenzyme is increased. The increased expression levels are achieved byoverexpressing an endogenous protein or by expressing a heterologousprotein.

Other modifications to the recombinant cell that are part of thedisclosure are modifications that decrease the activity of genes orpathways in the recombinant cell. Preferably, the expression levels ofone, two, three or more of the genes for hexose kinase, MIG-1, MIG-2,XR, aldose reductase, and XDH are reduced. Reducing gene activity can beachieved by a targeted deletion or disruption of the gene (andoptionally reintroducing the gene under the control of a differentpromoter that drives lower levels of expression or inducibleexpression).

In yet other aspects, the disclosure is directed to methods of producingfermentation products, for example one or more of ethanol, butanol,diesel, lactic acid, 3-hydroxy-propionic acid, acrylic acid, aceticacid, succinic acid, citric acid, malic acid, fumaric acid, itaconicacid, an amino acid, 1,3-propane-diol, ethylene, glycerol, a β-lactamantibiotic and a cephalosporin. Typically, a cell that recombinantlyexpresses a XI of the disclosure is cultured in a xylose-containingmedium, for example a medium supplemented with a lignocellulosichydrolysate. The media may also contain glucose, arabinose, or othersugars, particularly those derived from lignocellulose. The media may beof any pH, particularly a pH between 3.0 and 9.0, preferably between 4.0and 8.0, more preferably between 5.0 and 8.0, even more preferablybetween 6.0 and 7.5. The culture may occur in any media where theculture is under anaerobic or aerobic conditions, preferably underanaerobic conditions for production of compounds mentioned above andaerobically for biomass/cellular production. Optionally, the methodsfurther comprise recovering the fermentation product produced by therecombinant cell.

3. BRIEF DESCRIPTION OF THE FIGURES

FIGS. 1A-1B are maps for the vector pMEV-ΔxylA (MEV3 xylA del) andPCR-BluntII-TOPO-xylA, respectively, used in the activity-based screenfor XIs.

FIG. 2 illustrates the experimental strategy for the two-step markerexchange approach.

FIG. 3 is a map of the vector p426PGK1 for expressing XI in yeaststrain, Saccharomyces cerevisiae CEN.PK2-1Ca (ATCC: MYA1108).

FIG. 4 shows the growth rates on xylose containing media of selectedclones expressed in yeast strain, Saccharomyces cerevisiae CEN.PK2-1Ca(ATCC: MYA1108).

FIGS. 5A-5D are maps for the vectors pYDAB-006, pYDURA01, pYDPt-005 andpYDAB-0006, respectively, all used in creating strains of industrial S.cerevisiae strain yBPA130 with a single genomic copy of select XIclones.

FIG. 6 is a map of vector YDAB008-rDNA for multiple XI integration intoS. cerevisiae strain yBPB007 and yBPB008.

FIGS. 7A-7B show monosaccharide (including xylose) utilization andethanol production by strains of industrial S. cerevisiae with multiplecopies of XI clones integrated into ribosomal DNA loci.

FIG. 8: Production of ethanol from glycolytic and pentose phosphate(“PPP”) pathways. Not all steps are shown. For example,glyceraldehyde-3-phosphate is converted to pyruvate via a series ofglycolytic steps: (1) glyceraldehyde-3-phosphate to3-phospho-D-glycerol-phosphate catalyzed by glyceraldehyde-3-phosphatedehydrogenase (TDH1-3); (2) 3-phospho-D-glycerol-phosphate to3-phosphoglycerate catalyzed by 3-phosphoglycerate kinase (PGK1); (3)3-phosphoglycerate to 2-phosphoglycerate catalyzed by phosphoglyceratemutase (GPM1); (4) 2-phosphoglycerate to phosphoenolpyruvate catalyzedby enolase (ENO1; ENO2); and (5) phosphoenolpyruvate to pyruvatecalatyzed by pyruvate kinase (PYK2; CDC19). Other abbreviations:DHAP=dihydroxy-acetone-phosphate; GPD=Glycerol-3-phosphatedehydrogenase; RHR2/HOR2=DL-glycerol-3-phosphatase; XI=xylose isomerase;GRE=xylose reductase/aldose reductase; XYL=xylitol dehydrogenase;XKS=xylulokinase; PDC=pyruvate decarboxylase; ADH=alcohol dehydrogenase;ALD=aldehyde dehydrogenase; FIXK=hexokinase; PGI=phosphoglucoseisomerase; PFK=phosphofructokinase; FBA=aldolase; TPI=triosephosphateisomerase; ZWF=glucose-6 phosphate dehydrogenase;SOL=6-phosphogluconolactonase; GND=6-phosphogluconate dehydrogenase;RPE=D-ribulose-5-Phosphate 3-epimerase; RKI=ribose-5-phosphateketol-isomerase; TKL=transketolase; TAL=transaldolase. Heavy dashedarrows indicate reactions and corresponding enzymes that can be reducedor eliminated to increase xylose utilization, particularly in theproduction of ethanol, and heavy solid arrows indicate reactions andcorresponding enzymes that can be increased to increase xyloseutilization, particularly in the production of ethanol. The enzymesshown in FIG. 8 are encoded by S. cerevisiae genes. The S. cerevisiaegenes are used for exemplification purposes. Analogous enzymes andmodifications in other organisms are within the scope of the presentdisclosure.

4. DETAILED DESCRIPTION

4.1 Xylose Isomerase Polypeptides

A “xylose isomerase” or “XI” is an enzyme that catalyzes the directisomerisation of D-xylose into D-xylulose and/or vice versa. This classof enzymes is also known as D-xylose ketoisomerases. A xylose isomeraseherein may also be capable of catalyzing the conversion betweenD-glucose and D-fructose (and accordingly may therefore be referred toas a glucose isomerase).

A “XI polypeptide of the disclosure” or a “XI of the disclosure” is axylose isomerase having an amino acid sequence that is related to anyone of SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28,30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64,66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100,102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128,130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156,158, 160, 162, 164, 166, 168, 170, 172, 174, or 176. In someembodiments, the xylose isomerase of the disclosure has an amino acidsequence that is at least about 70%, at least 80%, at least 90%, atleast 95%, at least 96%, at least 98%, or at least 99% sequence identitythereto, or to a catalytic or dimerization domain thereof. The xyloseisomerase of the disclosure can also have 100% sequence identity to oneof the foregoing sequences.

The disclosure provides isolated, synthetic or recombinant XIpolypeptides comprising an amino acid sequence having at least about80%, e.g., at least about 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%,90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or complete (100%)sequence identity to a polypeptide of SEQ ID NO:2, 4, 6, 8, 10, 12, 14,16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50,52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86,88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116,118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144,146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172,174, or 176, over a region of at least about 10, e.g., at least about15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100,125, 150, 175, 200, 225, 250, 275, 300, 325, or 350 residues, or overthe full length of the polypeptide, over the length of catalytic domain,or over the length of the dimerization domain.

The XI polypeptides of the disclosure can be encoded by a nucleic acidsequence having at least about 80%, about 85%, about 86%, about 87%,about 88%, about 89%, or about 90% sequence identity to 1, 3, 5, 7, 9,11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45,47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81,83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113,115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141,143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169,171, 173, or 175, or by a nucleic acid sequence capable of hybridizingunder high stringency conditions to a complement of SEQ ID NO:1, 3, 5,7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41,43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77,79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109,111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137,139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165,167, 169, 171, 173, or 175, or to a fragment thereof. Exemplary nucleicacids of the disclosure are described in Section 4.2 below.

In specific embodiments, a polypeptide of the disclosure comprises anamino acid sequence having:

-   -   (1) (a) at least 97% or 98% sequence identity to SEQ ID NO:78 or        the catalytic domain thereof (amino acids 2-377 of SEQ ID NO:78)        and/or (b) at least 80%, 85%, 90%, 93% or 95% sequence identity        to SEQ ID NO:78 or the catalytic domain thereof (amino acids        2-377 of SEQ ID NO:78) and further comprises (i) SEQ ID NO:212        or SEQ ID NO:213 and/or (ii) SEQ ID NO:214;    -   (2) (a) at least 95%, 97% or 98% sequence identity to SEQ ID        NO:96 or the catalytic domain thereof (amino acids 2-377 of SEQ        ID NO:96) and/or (b) at least 80%, 85%, 90%, 93% or 95% sequence        identity to SEQ ID NO:96 or the catalytic domain thereof (amino        acids 2-377 of SEQ ID NO:96) and further comprises (i) SEQ ID        NO:212 or SEQ ID NO:213 and/or (ii) SEQ ID NO:214;    -   (3) at least 80%, 85%, 90%, 93%, 95%, 97% or 98% sequence        identity to SEQ ID NO:38 or the catalytic domain thereof (amino        acids 2-374 of SEQ ID NO:38), and optionally further comprises        one, two, three, four or all five of (i) SEQ ID NO:206 or SEQ ID        NO:207; (ii) SEQ ID NO:208; (iii) SEQ ID NO:209; (iv) SEQ ID        NO:210; and (iv) SEQ ID NO:211;    -   (4) at least 80%, 85%, 90%, 93%, 95%, 97% or 98% sequence        identity to SEQ ID NO:2 or the catalytic domain thereof (amino        acids 2-374 of SEQ ID NO:2);    -   (5) at least 93%, 95%, 97% or 98% sequence identity to SEQ ID        NO:58 or the catalytic domain thereof (amino acids 2-376 of SEQ        ID NO:58),    -   (6) at least 80%, 85%, 90%, 93%, 95%, 97% or 98% sequence        identity to SEQ ID NO:42 or the catalytic domain thereof (amino        acids 2-375 of SEQ ID NO:42), and optionally further comprises        one, two or all three of (i) SEQ ID NO:206 or SEQ ID        NO:207; (ii) SEQ ID NO:210; and (iii) SEQ ID NO:211;    -   (7) (a) at least 97% or 98% sequence identity to SEQ ID NO:84 or        the catalytic domain thereof (amino acids 2-376 of SEQ ID        NO:84), and/or (b) at least 80%, 85%, 90%, 93% or 95% sequence        identity to SEQ ID NO:84 or the catalytic domain thereof (amino        acids 2-376 of SEQ ID NO:84) and further comprises (i) SEQ ID        NO:212 or SEQ ID NO:213 and/or (ii) SEQ ID NO:214;    -   (8) (a) at least 97% or 98% sequence identity to SEQ ID NO:80 or        the catalytic domain thereof (amino acids 2-377 of SEQ ID NO:80)        and/or (b) at least 80%, 85%, 90%, 93% or 95% sequence identity        to SEQ ID NO:80 or the catalytic domain thereof (amino acids        2-377 of SEQ ID NO:80) and further comprises (i) SEQ ID NO:212        or SEQ ID NO:213 and/or (ii) SEQ ID NO:214;    -   (9) at least 93%, 95%, 97% or 98% sequence identity to SEQ ID        NO:54 or the catalytic domain thereof (amino acids 2-376 of SEQ        ID NO:54);    -   (10) at least 80%, 85%, 90%, 93%, 95%, 97% or 98% sequence        identity to SEQ ID NO:46 or the catalytic domain thereof (amino        acids 2-376 of SEQ ID NO:46), and optionally further comprises        SEQ ID NO:206 or SEQ ID NO:207;    -   (11) at least 90%, 93%, 95%, 97% or 98% sequence identity to SEQ        ID NO:16 or the catalytic domain thereof (amino acids 2-376 of        SEQ ID NO:16);    -   (12) at least 85%, 90%, 93%, 95%, 97% or 98% sequence identity        to SEQ ID NO:82 or the catalytic domain thereof (amino acids        2-375 of SEQ ID NO:82); and/or    -   (13) at least 90%, 93%, 95%, 97% or 98% sequence identity to SEQ        ID NO:32 or the catalytic domain thereof (amino acids 2-377 of        SEQ ID NO:32).

An example of an algorithm that is suitable for determining sequencesimilarity is the BLAST algorithm, which is described in Altschul etal., 1990, J. Mol. Biol. 215:403-410. Software for performing BLASTanalyses is publicly available through the National Center forBiotechnology Information. This algorithm involves first identifyinghigh scoring sequence pairs (HSPs) by identifying short words of lengthW in the query sequence that either match or satisfy somepositive-valued threshold score T when aligned with a word of the samelength in a database sequence. These initial neighborhood word hits actas starting points to find longer HSPs containing them. The word hitsare expanded in both directions along each of the two sequences beingcompared for as far as the cumulative alignment score can be increased.Extension of the word hits is stopped when: the cumulative alignmentscore falls off by the quantity X from a maximum achieved value; thecumulative score goes to zero or below; or the end of either sequence isreached. The BLAST algorithm parameters W, T, and X determine thesensitivity and speed of the alignment. The BLAST program uses asdefaults a word length (W) of 11, the BLOSUM62 scoring matrix (seeHenikoff & Henikoff, 1992, Proc. Nat'l. Acad. Sci. USA 89:10915-10919)alignments (B) of 50, expectation (E) of 10, M′5, N′-4, and a comparisonof both strands.

Any of the amino acid sequences described herein can be producedtogether or in conjunction with at least 1, e.g., at least (or up to) 2,3, 5, 10, or 20 heterologous amino acids flanking each of the C- and/orN-terminal ends of the specified amino acid sequence, and or deletionsof at least 1, e.g., at least (or up to) 2, 3, 5, 10, or 20 amino acidsfrom the C- and/or N-terminal ends of a XI of the disclosure.

The XIs of the disclosure can be characterized in terms of theiractivity. In some embodiments, a XI of the disclosure has at least 1.3times the activity of the Orpinomyces sp. XI assigned Genbank AccessionNo. 169733248 (“Op-XI”) at pH 7.5, for example using the assay describedin any of Examples 4, 6 and 7. In certain specific embodiments, a XI ofthe disclosure has an activity ranging from 1.25 to 3.0 times, from 1.5to 3 times, from 1.5 to 2.25 times, or from 1.75 to 3 times the activityof Op-XI at pH 7.5.

The XIs of the disclosure can also be characterized in terms of theirtolerance to acidic environments (e.g., at a pH of 6.5 or 6). In someembodiments, a XI of the disclosure has at least 1.9 times the activityof the Op-XI at pH 6, for example using the assay described in Example7. In certain specific embodiments, a XI of the disclosure has anactivity ranging from 1.9 to 4.1 times, from 2.4 to 4.1 times, from 2.4to 3.9 times, or 2.4 to 4.1 times the activity of Op-XI at pH6.

Tolerance to acidic environments can also be characterized as a ratio ofactivity at pH 6 to activity at pH 7.5 (“a pH 6 to pH 7.5 activityratio”), for example as measured using the assay of Example 7. In someembodiments, the pH 6 to pH 7.5 activity ratio is at least 0.5 or atleast 0.6. In various embodiments, the pH 6 to pH 7.5 activity ratio is0.5-0.9 or 0.6-0.9.

The xylose isomerases of the disclosure can have one or more (e.g., upto 2, 3, 5, 10, or 20) conservative amino acid substitutions relative tothe polypeptide of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24,26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60,62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96,98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124,126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152,154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, or 176 or to theportion thereof of discussed above. The conservative substitutions canbe chosen from among a group having a similar side chain to thereference amino acid. For example, a group of amino acids havingaliphatic side chains is glycine, alanine, valine, leucine, andisoleucine; a group of amino acids having aliphatic-hydroxyl side chainsis serine and threonine; a group of amino acids having amide-containingside chains is asparagine and glutamine; a group of amino acids havingaromatic side chains is phenylalanine, tyrosine, and tryptophan; a groupof amino acids having basic side chains is lysine, arginine, andhistidine; and a group of amino acids having sulphur-containing sidechains is cysteine and methionine. Accordingly, exemplary conservativesubstitutions for each of the naturally occurring amino acids are asfollows: ala to ser; arg to lys; asn to gln or his; asp to glu; cys toser or ala; gln to asn; glu to asp; gly to pro; his to asn or gln; ileto leu or val; leu to ile or val; lys to arg; gln or glu; met to leu orile; phe to met, leu or tyr; ser to thr; thr to ser; trp to tyr; tyr totrp or phe; and, val to ile or leu.

The present disclosure also provides a fusion protein that includes atleast a portion (e.g., a fragment or domain) of a XI polypeptide of thedisclosure attached to one or more fusion segments, which are typicallyheterologous to the XI polypeptide. Suitable fusion segments include,without limitation, segments that can provide other desirable biologicalactivity or facilitate purification of the XI polypeptide (e.g., byaffinity chromatography). Fusion segments can be joined to the amino orcarboxy terminus of a XI polypeptide. The fusion segments can besusceptible to cleavage.

4.2 Xylose Isomerase Nucleic Acids

A “XI nucleic acid of the disclosure” is a nucleic acid encoding axylose isomerase of the disclosure. In certain embodiments, the xyloseisomerase nucleic acid of the disclosure is encoded by a nucleotidesequence of any one of SEQ ID NOs:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21,23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57,59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93,95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123,125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151,153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, or 175, or asequence having at least about 70%, at least 80%, at least 90%, at least95%, at least 96%, at least 98%, or at least 99% sequence identitythereto. The xylose isomerase nucleic acid of the disclosure can alsohave 100% sequence identity to one of the foregoing sequences.

The present disclosure provides nucleic acids encoding a polypeptide ofthe disclosure, for example one described in Section 4.1 above. Thedisclosure provides isolated, synthetic or recombinant nucleic acidscomprising a nucleic acid sequence having at least about 70%, e.g., atleast about 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%,83%, 84%, 85%, 86%, 87%, 88%; 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%,97%, 98%, or 99%, or complete (100%) sequence identity to a nucleic acidof SEQ ID NO:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31,33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67,69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101,103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129,131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157,159, 161, 163, 165, 167, 169, 171, 173, or 175, over a region of atleast about 10, e.g., at least about 15, 20, 25, 30, 35, 40, 45, 50, 75,100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750,800, 850, 900, 950, 1000, 1050, 1100, 1150, 1200, 1250, 1300, 1350,1400, 1450, 1500, 1550, 1600, 1650, 1700, 1750, 1800, 1850, 1900, 1950,or 2000 nucleotides.

Nucleic acids of the disclosure also include isolated, synthetic orrecombinant nucleic acids encoding a XI polypeptide having the sequenceof SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32,34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68,70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102,104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130,132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158,160, 162, 164, 166, 168, 170, 172, 174, or 176, and subsequences thereof(e.g., a conserved domain or a catalytic domain), and variants thereof.

To increase the likelihood that a XI polypeptide is recombinantlyexpressed, a XI nucleic acid may be adapted to optimize its codon usageto that of the chosen cell. Several methods for codon optimization areknown in the art. For expression in yeast, an exemplary method tooptimize codon usage of the nucleotide sequences to that of the yeast isa codon pair optimization technology as disclosed in WO2006/077258and/or WO2008/000632. WO2008/000632 addresses codon-pair optimization.Codon-pair optimization is a method wherein the nucleotide sequencesencoding a polypeptide are modified with respect to their codon-usage,in particular the codon-pairs that are used, to obtain improvedexpression of the nucleotide sequence encoding the polypeptide and/orimproved production of the encoded polypeptide. Codon pairs are definedas a set of two subsequent triplets (codons) in a coding sequence.

4.3 Host Cells and Recombinant Expression

The disclosure also provides host cells transformed with a XI nucleicacid and recombinant host cells engineered to express XI polypeptides.The XI nucleic acid construct may be extrachromosomal, on a plasmid,which can be a low copy plasmid or a high copy plasmid. The nucleic acidconstruct may be maintained episomally and thus comprise a sequence forautonomous replication, such as an autosomal replication sequence.Alternatively, a XI nucleic acid may be integrated in one or more copiesinto the genome of the cell. Integration into the cell's genome mayoccur at random by non-homologous recombination but preferably, thenucleic acid construct may be integrated into the cell's genome byhomologous recombination as is well known in the art. In certainembodiments, the host cell is bacterial or fungal (e.g., a yeast or afilamentous fungus).

Suitable host cells of the bacterial genera include, but are not limitedto, cells of Escherichia, Bacillus, Lactobacillus, Pseudomonas, andStreptomyces. Suitable cells of bacterial species include, but are notlimited to, cells of Escherichia coli, Bacillus subtilis, Bacilluslicheniformis, Lactobacillus brevis, Pseudomonas aeruginosa, andStreptomyces lividans.

Suitable host cells of the genera of yeast include, but are not limitedto, cells of Saccharomyces, Kluyveromyces, Candida, Pichia,Schizosaccharomyces, Hansenula, Klockera, Schwanniomyces, Phaffia,Issatchenkia and Yarrowia. In specific embodiments, the recombinant cellis a S. cerevisiae, C. albicans, S. pombe, S. bulderi, S. barnetti, S.exiguus, S. uvarum, S. diastaticus, H. polymorpha, K. lactis, I.orientalis, K. marxianus, K. fragilis, P. pastoris, P. canadensis, K.marxianus or P. rhodozyma. Exemplary yeast strains that are suitable forrecombinant XI expression include, but are not limited to, LallemandLYCC 6391, Lallemand LYCC 6939, Lallemand LYCC 6469, (all fromLallemand, Inc., Montreal, Canada); NRRL YB-1952 (ARS (NRRL) Collection,U.S. Department of Agriculture); and BY4741.

Suitable host cells of filamentous fungi include all filamentous formsof the subdivision Eumycotina. Suitable cells of filamentous fungalgenera include, but are not limited to, cells of Acremonium,Aspergillus, Aureobasidium, Bjerkandera, Ceriporiopsis, Chrysoporium,Coprinus, Coriolus, Corynascus, Chaetomium, Cryptococcus, Filobasidium,Fusarium, Gibberella, Humicola, Hypocrea, Magnaporthe, Mucor,Myceliophthora, Neocallimastix, Neurospora, Paecilomyces, Penicillium,Phanerochaete, Phlebia, Piromyces, Pleurotus, Scytaldium, Schizophyllum,Sporotrichum, Talaromyces, Thermoascus, Thielavia, Tolypocladium,Trametes, and Trichoderma. In certain aspects, the recombinant cell is aTrichoderma sp. (e.g., Trichoderma reesei), Penicillium sp., Humicolasp. (e.g., Humicola insolens); Aspergillus sp. (e.g., Aspergillusniger), Chrysosporium sp., Fusarium sp., or Hypocrea sp. Suitable cellscan also include cells of various anamorph and teleomorph forms of thesefilamentous fungal genera.

Suitable cells of filamentous fungal species include, but are notlimited to, cells of Aspergillus awamori, Aspergillus fumigatus,Aspergillus foetidus, Aspergillus japonicus, Aspergillus nidulans,Aspergillus niger, Aspergillus oryzae, Chrysosporium lucknowense,Fusarium bactridioides, Fusarium cerealis, Fusarium crookwellense,Fusarium culmorum, Fusarium graminearum, Fusarium graminum, Fusariumheterosporum, Fusarium negundi, Fusarium oxysporum, Fusariumreticulatum, Fusarium roseum, Fusarium sambucinum, Fusarium sarcochroum,Fusarium sporotrichioides, Fusarium sulphureum, Fusarium torulosum,Fusarium trichothecioides, Fusarium venenatum, Bjerkandera adusta,Ceriporiopsis aneirina, Ceriporiopsis aneirina, Ceriporiopsis caregiea,Ceriporiopsis gilvescens, Ceriporiopsis pannocinta, Ceriporiopsisrivulosa, Ceriporiopsis subrufa, Ceriporiopsis subvermispora, Coprinuscinereus, Coriolus hirsutus, Humicola insolens, Humicola lanuginosa,Mucor miehei, Myceliophthora thermophila, Neurospora crassa, Neurosporaintermedia, Penicillium purpurogenum, Penicillium canescens, Penicilliumsolitum, Penicillium funiculosum, Phanerochaete chrysosporium, Phlebiaradiate, Pleurotus eryngii, Talaromyces flavus, Thielavia terrestris,Trametes villosa, Trametes versicolor, Trichoderma harzianum,Trichoderma koningii, Trichoderma longibrachiatum, Trichoderma reesei,and Trichoderma viride.

Typically, for recombinant expression, the XI nucleic acid will beoperably linked to one or more nucleic acid sequences capable ofproviding for or aiding the transcription and/or translation of the XIsequence, for example a promoter operable in the organism in which theXI is to be expressed. The promoters can be homologous or heterologous,and constitutive or inducible.

Preferably, the XI polypeptide is expressed in the cytosol and thereforelacks a mitochondrial or peroxisomal targeting signal.

Where recombinant expression in a filamentous fungal host is desired,the promoter can be a fungal promoter (including but not limited to afilamentous fungal promoter), a promoter operable in plant cells, apromoter operable in mammalian cells.

As described in U.S. provisional application No. 61/553,901, filed Oct.31, 2011, the contents of which are hereby incorporated in theirentireties, promoters that are constitutively active in mammalian cells(which can derived from a mammalian genome or the genome of a mammalianvirus) are capable of eliciting high expression levels in filamentousfungi such as Trichoderma reesei. An exemplary promoter is thecytomegalovirus (“CMV”) promoter.

As described in U.S. provisional application No. 61/553,897, filed Oct.31, 2011, the contents of which are hereby incorporated in theirentireties, promoters that are constitutively active in plant cells(which can derived from a plant genome or the genome of a plant virus)are capable of eliciting high expression levels in filamentous fungisuch as Trichoderma reesei. Exemplary promoters are the cauliflowermosaic virus (“CaMV”) 35S promoter or the Commelina yellow mottle virus(“CoYMV”) promoter.

Mammalian, mammalian viral, plant and plant viral promoters can driveparticularly high expression when the associated 5′ UTR sequence (i.e.,the sequence which begins at the transcription start site and ends onenucleotide (nt) before the start codon), normally associated with themammalian or mammalian viral promoter is replaced by a fungal 5′ UTRsequence.

The source of the 5′ UTR can vary provided it is operable in thefilamentous fungal cell. In various embodiments, the 5′ UTR can bederived from a yeast gene or a filamentous fungal gene. The 5′ UTR canbe from the same species, one other component in the expression cassette(e.g., the promoter or the XI coding sequence), or from a differentspecies. The 5′ UTR can be from the same species as the filamentousfungal cell that the expression construct is intended to operate in. Inan exemplary embodiment, the 5′ UTR comprises a sequence correspondingto a fragment of a 5′ UTR from a T. reesei glyceraldehyde-3-phosphatedehydrogenase (gpd). In a specific embodiment, the 5′ UTR is notnaturally associated with the CMV promoter

Examples of other promoters that can be used include, but are notlimited to, a cellulase promoter, a xylanase promoter, the 1818 promoter(previously identified as a highly expressed protein by EST mappingTrichoderma). For example, the promoter can suitably be acellobiohydrolase, endoglucanase, or β-glucosidase promoter. Aparticularly suitable promoter can be, for example, a T. reeseicellobiohydrolase, endoglucanase, or β-glucosidase promoter.Non-limiting examples of promoters include a cbh1, cbh2, egl1, egl2,egl3, egl4, egl5, pkil, gpdl, xyn1, or xyn2 promoter.

For recombinant expression in yeast, suitable promoters for S.cerevisiae include the MFa1 promoter, galactose inducible promoters suchas the GAL1, GAL7 and GAL10 promoters, glycolytic enzyme promotersincluding the TPI and PGK promoters, the TDH3 promoter, the TEF1promoter, the TRP1 promoter, the CYCI promoter, the CUP1 promoter, thePHOS promoter, the ADH1 promoter, and the HSP promoter. Promoters thatare active at different stage of growth or production (e.g., idiophaseor trophophase) can also be used (see, e.g., Puig et al., 1996,Biotechnology Letters 18(8):887-892; Puig and Perez-Ortin, 2000,Systematic and Applied Microbiology 23(2):300-303; Simon et al., 2001,Cell 106:697-708; Wittenberg and Reed, 2005, Oncogene 24:2746-2755). Asuitable promoter in the genus Pichia sp. is the AOXI (methanolutilization) promoter.

The engineered host cells can be cultured in conventional nutrient mediamodified as appropriate for activating promoters, selectingtransformants, or amplifying the nucleic acid sequence encoding the XIpolypeptide. Culture conditions, such as temperature, pH and the like,are those previously used with the host cell selected for expression,and will be apparent to those skilled in the art. As noted, manyreferences are available for the culture and production of many cells,including cells of bacterial and fungal origin. Cell culture media ingeneral are set forth in Atlas and Parks (eds.), 1993, The Handbook ofMicrobiological Media, CRC Press, Boca Raton, Fla., which isincorporated herein by reference. For recombinant expression infilamentous fungal cells, the cells are cultured in a standard mediumcontaining physiological salts and nutrients, such as described inPourquie et al., 1988, Biochemistry and Genetics of CelluloseDegradation, eds. Aubert, et al., Academic Press, pp. 71-86; and Ilmenet al., 1997, Appl. Environ. Microbiol. 63:1298-1306. Culture conditionsare also standard, e.g., cultures are incubated at 30° C. in shakercultures or fermenters until desired levels of XI expression areachieved. Preferred culture conditions for a given filamentous fungusmay be found in the scientific literature and/or from the source of thefungi such as the American Type Culture Collection (ATCC). After fungalgrowth has been established, the cells are exposed to conditionseffective to cause or permit the expression of a XI.

In cases where a XI coding sequence is under the control of an induciblepromoter, the inducing agent, e.g., a sugar, metal salt or antibiotics,is added to the medium at a concentration effective to induce XIexpression.

In addition to recombinant expression of a XI polypeptide, a host cellof the disclosure may further include one or more genetic modificationsthat increase the cell's ability to utilize xylose as a substrate in afermentation process. Exemplary additional modifications create one,two, three, four, five or even more of the following phenotypes: (a)increase in xylose transport into the cell; (b) increase in aerobicgrowth rate on xylose; (c) increase in xylulose kinase activity; (d)increase in flux through the pentose phosphate pathway into glycolysis,(e) modulating in aldose reductase activity, (f) decrease in sensitivityto catabolite repression, (g) increase in tolerance to biofuels, e.g.,ethanol, (h) increase tolerance to intermediate production (for examplexylitol), (i) increase in temperature tolerance, (j) osmolarity oforganic acids, and (k) a reduced production of byproducts.

As illustrated below, a modification that results in one or more of theforegoing phenotypes can be a result of increasing or decreasingexpression of an endogenous protein (e.g., by at least a factor of about1.1, about 1.2, about 1.5, about 2, about 5, about 10 or about 20) or aresult of introducing expression of a heterologous polypeptide. Foravoidance of doubt, “decreasing” or “reducing” gene expressionencompasses eliminating expression. Decreasing (or reducing) theexpression of an endogenous protein can be accomplished by inactivatingone or more (or all) endogenous copies of a gene in a cell. A gene canbe inactivated by deletion of at least part of the gene or by disruptionof the gene. This can be achieved by deleting the some or all of a genecoding sequence or regulatory sequence whose deletion results in areduction of gene expression in the cell. Examples of modifications thatincrease xylose utilization or yield of fermentation product aredescribed below.

Increasing Xylose Transport:

Xylose transport can be increased directly or indirectly. For example, arecombinant cell may include one or more genetic modifications thatresult in expression of a xylose transporter. Exemplary transportersinclude, but are not limited to GXF1, SUT1 and At6g59250 from Candidaintermedia, Pichia stipitis (now renamed Scheffersomyces stipitis; theterms are used interchangeably herein) and Arabidopsis thaliana,respectively (Runquist et al., 2010, Biotechnol. Biofuels 3:5), as wellas HXT4, HXT5, HXT7, GAL2, AGT1, and GXF2 (see, e.g., Matsushika et al.,2009, Appl. Microbiol. Biotechnol. 84:37-53). Other transporters includePsAraT, SUT2-4 and XUT1-5 from P. stiptis; GXS1 from Candida intermedia;Xy1HP and DEHAOD02167 from Debaryomyces hansenii; and YALI0C06424 fromYarrowia lipolytica (see, e.g., Young et al., 2011, Appl. Environ.Microbiol. 77:3311-3319). Xylose transport can also be increased by(over-) expression of low-affinity hexose transporters, which arecapable of non-selectively transporting sugars, including xylose, intothe cell once glucose levels are low (e.g., 0.2-1.0 g/l); and includesCgHXT1-CgHXTS from Colletotrichum graminicola. The foregoingmodifications can be made singly or in combinations of two, three ormore modifications.

Increasing Xylulose Kinase Activity:

Xylulose kinase activity can be increased by overexpression of axylulose kinase, e.g., xylulose kinase (XKS1; Saccharomyces genomedatabase (“SGD”) accession no. YGR194C) of S. cerevisiae, particularlywhere the recombinant cell is a yeast cell. In one embodiment, a S.cerevisiae cell is engineered to include at least 2 additional copies ofxylulose kinase under the control of a strong constitutive promoter suchas TDH3, TEF1 or PGK1. In another embodiment, overexpression of anendogenous xylulose kinase was engineered. This xylulose kinase havingimproved kinetic activities through the use of protein engineeringtechniques known by those skilled in the art.

Increasing Flux Through the Pentose Phosphate Pathway:

This can be achieved by increasing expression of one or more genes inthe pentose phosphate pathway, for example S. cerevisiae transaldolaseTAL1 (SGD accession no. YLR354C), transketolase TKL1 (SGD accessionno.YPRO74C), ribulose 5-phosphate epimerase RPE1 (SGD accession no.YJL121C) and ribose-5-phosphate ketoisomerase RKI1 (SGD accession no.YOR095C) and/or one or more genes to increase glycolytic flux, forexample S. cerevisiae pyruvate kinase PYK1/CDC19 (SGD accession no.YAL038W), pyruvate decarboxylase PDC1 (SGD accession no. YLR044C),pyruvate decarboxylase PDC5 (SGD accession no. YLR134W), pyruvatedecarboxylase PDC6 (SGD accession no. YGRO87C), the alcoholdehydrogenases ADH1-5 (SGD accession nos. YOL086C, YMR303C, YMR083W,YGL256W, and YBR145W, respectively), and hexose kinase HXK1-2 (SGDaccession nos. YFRO53C and YGL253W, respectively). In one embodiment,the yeast cell has one additional copy each of TAL1, TKL1, RPE1 and RKI1from S. cerevisiae under the control of strong constitutive promoters(e.g., PGK1, TDH3, TEF1); and may also include improvements toglycolytic flux (e.g., increased copies of genes such as PYK1, PDC1,PDC5, PDC6, ADH1-5) and glucose-6-phosphate and hexokinase. Theforegoing modifications can be made singly or in combinations of two,three or more modifications.

Modulating Aldose Reductase Activity:

A recombinant cell can include one or more genetic modifications thatincrease or reduce (unspecific) aldose reductase (sometimes calledaldo-keto reductase) activity. Aldose reductase activity can be reducedby one or more genetic modifications that reduce the expression of orinactivate a gene encoding an aldose reductase, for example S.cerevisiae GRE3 (SGD accession no. YHR104W).

In certain embodiments, GRE3 expression is reduced. In one aspect, therecombinant cell is a yeast cell in which the GRE3 gene is deleted.Deletion of GRE3 decreased xylitol yield by 49% and biomass productionby 31%, but increased ethanol yield by 19% (Traff-Bjerre et al., 2004,Yeast 21:141-150). In another aspect, the recombinant cell is a yeastcell which has a reduction in expression of GRE3. Reducing GRE3expression has been shown to result in a two-fold decrease in by-product(i.e., xylitol) formation and an associated improvement in ethanol yield(Traff et al., 2001, Appl. Environ. Microbiol. 67:5668-5674).

In another embodiment, the recombinant cell is a cell (optionally butnot necessarily a yeast cell) in which GRE3 is overexpressed. In a studyanalyzing the effect of GRE3 overexpression in S. cerevisiae toinvestigate the effect on xylose utilization, an increase of about 30%in xylose consumption and about 120% in ethanol production was noted(Traff-Bjerre et al., 2004, Yeast 21:141-150).

Decreasing Xylose Reductase Activity:

A recombinant cell may include one or more genetic modifications thatreduce xylose reductase activity. Xylose reductase activity can bereduced by one or more genetic modifications that reduce the expressionof or inactivate a gene encoding a xylose reductase.

Decreasing Sensitivity to Catabolite Repression:

Glucose and other sugars, such as galactose or maltose, are able tocause carbon catabolite repression in Crabtree-positive yeast, such asS. cerevisiae. In one study, xylose was found to decrease thederepression of various enzymes of an engineered S. cerevisiae straincapable of xylose utilization by at least 10-fold in the presence ofethanol. Xylose also impaired the derepression of galactokinase andinvertase (Belinchon & Gancedo, 2003, Arch. Microbiol. 180:293-297). Incertain embodiments, in order to reduce catabolite sensitivity, yeastcan include one or more genetic modifications that reduce expression ofone or more of GRR1 (SGD accession no. YJR090C), the gene assigned SGDaccession no. YLR042C, GAT1 (SGD accession no.YKR067W) and/or one ormore genetic modifications that decrease expression of one or more ofSNF1 (SGD accession no. YDR477W), SNF4 (SGD accession no. YGL115W), MIG1(SGD accession no.YGL035C) and CRE1 (SGD accession no. YJL127C). Infurther embodiments, yeast can include one or more genetic modificationsthat result in overexpression of the pentose phosphate pathway enzymes.In yet further embodiments, yeast can include one or more geneticmodifications that reduce expression of hexo-/glucokinase. In yet afurther embodiment, yeast can include one or more genetic modificationsthat modulate the activity of one or more GATA factors, for exampleGAT1, DAL80 (SGD accession no. YKR034W), GZF3 (SGD accession no.YJL110C) and GLN3 (SGD accession no. YER040W). The foregoingmodifications can be made singly or in combinations of two, three ormore modifications.

Increasing Tolerance to Biofuels (e.g., Ethanol), Pathway Intermediates(e.g., Xylitol), Organic Acids and Temperature:

For efficient bioethanol production from lignocellulosic biomass, it isuseful to improve cellular tolerance to toxic compounds released duringthe pretreatment of biomass. In one study, the gene encoding PHO13 (SGDaccession no. YDL236W), a protein with alkaline phosphatase activity,was disrupted. This resulted in improved ethanol production from xylosein the presence of three major inhibitors (i.e., acetic acid, formicacid and furfural). Further, the specific ethanol productivity of themutant in the presence of 90 mM furfural was four fold higher (Fujitomiet al., 2012, Biores. Tech., 111:161-166). Thus, in one embodiment,yeast has one or more genetic modifications that reduce P11013expression. In other embodiments, yeast, bacterial and fungal cells areevolved under selective conditions to identify strains that canwithstand higher temperatures, higher levels of intermediates, higherlevels of organic acids and/or higher levels of biofuels (e.g.,ethanol). In yet other embodiments, yeast are engineered to reduceexpression of FPS1 (SGD accession no. YLL043W); overexpress unsaturatedlipid and ergosterol biosynthetic pathways; reduce expression of PHO13and/or SSK2 (SGD accession no. YNR031C); modulate global transcriptionfactor cAMP receptor protein, through increasing or decreasingexpression; increase expression of MSN2 (SGD accession no. YMR037C),RCN1 (SGD accession no. YKL159C), RSA3 (SGD accession no. YLR221C),CDC19 and/or ADH1; or increase expression of Rice ASR1. The foregoingmodifications can be made singly or in combinations of two, three ormore modifications.

Reducing Production of Byproducts:

Glycerol is one of the main byproducts in C6 ethanol production.Reducing glycerol is desirable for increasing xylose utilization byyeast. Production of glycerol can be reduced by deleting the geneencoding the FPS1 channel protein, which mediates glycerol export, andGPD2 (SGD accession no. YOL059W), which encodes glycerol-3-phosphatedehydrogenase; optionally along with overexpression of GLT1 (SGDaccession no. YDL171C) and GLN1 (SGD accession no. YPRO35W). In onestudy, FPS1 and GPD2 were knocked-out in one S. cerevisiae strain, andin another were replaced by overexpression of GLT1 and GLN1, whichencode glutamate synthase and glutamine synthetase, respectively. Whengrown under microaerobic conditions, these strains showed ethanol yieldimprovements of 13.17% and 6.66%, respectively. Conversely, glycerol,acetic acid and pyruvic acid were found to all decrease, with glyceroldown 37.4% and 41.7%, respectively (Zhang and Chen, 2008, Chinese J.Chem. Eng. 16:620-625).

Production of glycerol can also be reduced by deleting theNADH-dependent glycerol-3-phosphate dehydrogenase 1 (GPD1; SGD accessionno. YDL022W) and/or the NADPH-dependent glutamate dehydrogenase 1 (GDH1;SGD accession no. YOR375C). Sole deletion of GPD1 or GDH1 reducesglycerol production, and double deletion results in a 46.4% reduction ofglycerol production as compared to wild-type S. cerevisiae (Kim et al.,2012, Bioproc. Biosys. Eng. 35:49-54). Deleting FPS1 can decreaseproduction of glycerol for osmoregulatory reasons.

Reducing production of acetate can also increase xylose utilization.Deleting ALD6 (SGD accession no. YPL061W) can decrease production ofacetate.

ADM can also be deleted to reduce or eliminate acetylaldehyde formationfrom ethanol and thereby increase ethanol yield.

The foregoing modifications to reduce byproduct formation can be madesingly or in combinations of two, three or more modifications.

In addition to ethanol production, a recombinant XI-expressing cell ofthe disclosure can be suitable for the production of non-ethanolicfermentation products. Such non-ethanolic fermentation products includein principle any bulk or fine chemical that is producible by aeukaryotic microorganism such as a yeast or a filamentous fungus. Suchfermentation products may be, for example, butanol, lactic acid,3-hydroxy-propionic acid, acrylic acid, acetic acid, succinic acid,citric acid, malic acid, fumaric acid, itaconic acid, an amino acid,1,3-propane-diol, ethylene, glycerol, a β-lactam antibiotic or acephalosporin. A preferred modified host cell of the diclosure forproduction of non-ethanolic fermentation products is a host cell thatcontains a genetic modification that results in decreased alcoholdehydrogenase activity.

Cells expressing the XI polypeptides of the disclosure can be grownunder batch, fed-batch or continuous fermentations conditions. Classicalbatch fermentation is a closed system, wherein the compositions of themedium is set at the beginning of the fermentation and is not subject toartificial alternations during the fermentation. A variation of thebatch system is a fed-batch fermentation in which the substrate is addedin increments as the fermentation progresses. Fed-batch systems areuseful when catabolite repression is likely to inhibit the metabolism ofthe cells and where it is desirable to have limited amounts of substratein the medium. Batch and fed-batch fermentations are common and wellknown in the art. Continuous fermentation is an open system where adefined fermentation medium is added continuously to a bioreactor and anequal amount of conditioned medium is removed simultaneously forprocessing. Continuous fermentation generally maintains the cultures ata constant high density where cells are primarily in log phase growth.Continuous fermentation systems strive to maintain steady state growthconditions. Methods for modulating nutrients and growth factors forcontinuous fermentation processes as well as techniques for maximizingthe rate of product formation are well known in the art of industrialmicrobiology.

4.4 Fermentation Methods

A further aspect the disclosure relates to fermentation processes inwhich the recombinant XI-expressing cells are used for the fermentationof carbon source comprising a source of xylose. Thus, in certainembodiments, the disclosure provides a process for producing afermentation product by (a) fermenting a medium containing a source ofxylose with a recombinant XI-expressing cell as defined herein above,under conditions in which the cell ferments xylose to the fermentationproduct, and optionally, (b) recovery of the fermentation product. Insome embodiments, the fermentation product is an alcohol (e.g., ethanol,butanol, etc.), a fatty alcohol (e.g., a C8-C20 fatty alcohol), a fattyacid (e.g., a C8-C20 fatty acid), lactic acid, 3-hydroxypropionic acid,acrylic acid, acetic acid, succinic acid, citric acid, malic acid,fumaric acid, an amino acid, 1,3-propanediol, itaconic acid, ethylene,glycerol, and a β-lactam antibiotic such as Penicillin G or Penicillin Vand fermentative derivatives thereof and cephalosporins. Thefermentation process may be an aerobic or an anaerobic fermentationprocess.

In addition to a source of xylose the carbon source in the fermentationmedium may also comprise a source of glucose. The source of xylose orglucose may be xylose or glucose as such or may be any carbohydrateoligo- or polymer comprising xylose or glucose units, such as e.g.,lignocellulose, xylans, cellulose, starch and the like. Mostmicroorganisms possess carbon catabolite repression that results insequential consumption of mixed sugars derived from the lignocellulose,reducing the efficacy of the overall process. To increase the efficiencyof fermentation, microorganisms that are capable of simultaneousconsumption of mixed sugars (e.g., glucose and xylose) have beendeveloped, for example by rendering them less sensitive to glucoserepression (see, e.g., Kim et al., 2010, Appl. Microbiol. Biotechnol.88:1077-85 and Ho et al., 1999, Adv. Biochem. Eng. Biotechnol.65:163-92). Such cells can be used for recombinant XI expression and inthe fermentation methods of the disclosure.

The fermentation process is preferably run at a temperature that isoptimal for the recombinant XI-expressing cells. Thus, for most yeastsor fungal host cells, the fermentation process is performed at atemperature which is less than 38° C., unless temperature tolerantmutant strains are used, in which case the temperature may be higher.For most yeast or filamentous fungal host cells, the fermentationprocess is suitably performed at a temperature which is lower than 35°C., 33° C., 30° C. or 28° C. Optionally, the temperature is higher than20° C., 22° C., or 25° C.

An exemplary process is a process for the production of ethanol, wherebythe process comprises the steps of: (a) fermenting a medium containing asource of xylose with a transformed host cell as defined above, wherebythe host cell ferments xylose to ethanol; and optionally, (b) recoveryof the ethanol. The fermentation medium can also comprise a source ofglucose that is also fermented to ethanol. The source of xylose can besugars produced from biomass or agricultural wastes. Many processes forthe production of monomeric sugars such as glucose generated fromlignocellulose are well known, and are suitable for use herein. Inbrief, the cellulolytic material may be enzymatically, chemically,and/or physically hydrolyzed to a glucose and xylose containingfraction. Alternatively, the recombinant XI-expressing cells of thedisclosure can be further transformed with one or more genes encodingfor enzymes effective for hydrolysis of complex substrates such aslignocellulose, and include but are not limited to cellulases,hemicellulases, peroxidases, laccases, chitinases, proteases, andpectinases. The recombinant cells of the disclosure can then befermented under anaerobic in the presence of glucose and xylose. Wherethe recombinant cell is a yeast cell, the fermentation techniques andconditions described for example, by Wyman (1994, Biores. Technol.50:3-16) and Olsson and Hahn-Hagerdal (1996, Enzyme Microb. Technol.18:312-331) can be used. After completion of the fermentation, theethanol may be recovered and optionally purified or distilled. Solidresidue containing lignin may be discarded or burned as a fuel.

The fermentation process may be run under aerobic and anaerobicconditions. In some embodiments, the process is carried out undermicroaerobic or oxygen limited conditions. Fermentation can be carriedout in a batch, fed-batch, or continuous configuration within(bio)reactors.

5. EXAMPLES 5.1 Materials and Methods

5.1.1 Yeast Culture

Unless stated otherwise for a particular example, yeast transformantswere grown in SC-ura media with about 2% glucose at 30° C. for about 24hours. The media contains approx. 20 g agar, approx. 134 g BD Difco™Yeast Nitrogen Base without amino acids (BD, Franklin Lakes, N.J., andapprox. 2 g SC amino-acid mix containing about 85 mg of the followingamino acids unless noted (quantity listed in parentheses): L-Adenine(21.0), L-Alanine, L-Arginine, L-Asparagine, L-Aspartic Acid,L-Cysteine, Glutamine, L-Glutamic Acid, Glycine, L-Histidine,Myo-Inositol, L-Isoleucine, L-Leucine (173.4), L-Lysine, L-Methionine,p-Aminobenzoic Acid (8.6), L-Phenylalanine, L-Proline, L-Serine,L-Threonine, L-Tryptophan, L-Tyrosine, L-Valine).

5.1.2 Xylose Isomerase Activity

XI activity in cell lysates was determined using a method based on thatof Kersters-Hilderson et al., 1986, Enzyme Microb. Technol. 9:145-148,in which enzymatic conversion of xylose to xylulose by the XI is coupledwith the enzymatic conversion of the product (xylulose) to xylitol viathe enzyme sorbitol dehydrogenase (SDH). SDH activity requires theoxidation of NADH to NAD⁺. The rate of oxidation of NADH is directlyproportional to the rate of SDH conversion of D-xylulose to D-xylitoland is measured by the decrease in absorbance at 340 nm. One unit ofenzyme activity as measured by this assay is a decrease of 1 μmole ofNADH per minute under assay conditions. All reactions, solutions,plates, and spectrophotometer were equilibrated to about 35° C. prior touse. Assays were performed either on fresh lysates immediately afterpreparation or lysates that had been frozen at −20° C. immediately afterpreparation. Assays were performed using a BioTek Model: Synergy H1Hybrid Reader spectrophotometer and 96-well plates (Corning, Model#Costar® #3598). All spectrophotometric readings were performed at 340nm. A standard curve of NADH was generated with each assay withconcentrations ranging from 0 to about 0.6 mM.

The reaction buffer used for experiments at pH 7.5 was about 100 mMTris-HCl (pH 7.5). The assay mix was prepared as follows: reactionbuffer to which was added about 10 mM MgCl₂, 0.15 mM NADH and 0.05 mg/mlSDH (Roche, catalog #50-720-3313). For experiments where activity wasalso measured at pH 6, the buffer was changed to about 100 mM sodiumphosphate, pH 6. The assay mix for the entire experiment was thenprepared as follows: about 10 mM MgCl₂, 1.2 mM NADH and 0.02 mg/ml SDH.

Any sample dilutions were performed using the reaction buffer asdiluent. Reactions were set up by aliquotting about 90 μl of assay mixinto each well of the plates. About 10 μl of each XI sample was added tothe wells. The reactions were started by the addition of about 100 μlsubstrate solution (about 1 M D-xylose). Reactions were mixed and readimmediately using kinetic assay mode for about 10 minutes. Volumetricactivity (VA) units are in milli-absorbance (mA) units per minute per mlof lysate added to the reactions (mA/min/ml). Background VA rates ofnegative control wells (no enzyme added) were subtracted from VA ofsamples. Determination of fold improvement over positive control (FIOPC)was obtained by dividing the VA of the XI-samples by the VA observed fora control (Orpinomyces xylose isomerase, NCBI:169733248 (Op-XI))expressed using the same host and expression vector. In somecharacterizations, the slope of an NADH standard curve was used toconvert VA (mA/min) to μmole-NADH/min (or Units). If proteinquantitation was performed, specific activities (SA) were calculatedwhere the units for SA are (μmole NADH⁺/min/mg, or U/mg lysate protein).All activities listed (VA or SA) account for any dilutions, volumes oflysate added, and protein concentrations for the lysates assayed.

5.2 Example 2 Activity-Based Discovery Screen for Xylose Isomerases

Libraries used for the activity-based discovery (“ABD”) screen were inthe format of excised phagemids. These libraries were constructed asdescribed in U.S. Pat. No. 6,280,926. Sources for these libraries wereenvironmental rumen samples collected from the foregut of deceasedherbivores.

An Escherichia coli screening strain was constructed to identify genesfrom the environmental libraries encoding xylose isomerase activity.Specifically, E. coli strain SEL700, a MG1655 derivative that is recA⁻,phage lambda resistant and contains an F′ plasmid, was complemented withplasmid pJC859, a derivative of pBR322 containing the E. coli recA gene(Kokjohn et al., 1987, J. Bacteriol. 169:1499-1508) to generate awild-type recA phenotype.

A two-step marker exchange procedure was then used to delete the entirecoding sequence of the endogenous xylA xylose isomerase gene. Briefly,pMEV3, a plasmid with a pir-dependent replicon (ori6RK) encodingkanamycin-resistance and the sacB levansucrase, was used as a vector forconstruction of the xylA deletion plasmid. A fragment of DNA containingthe flanking regions of the xylA gene (0.7 kb of sequence 5′ and 0.9 kbof sequence 3′ of xylA) and containing BsaI restriction sites wasgenerated by overlap extension PCR using primers, ligated to pMEV3digested with Bbsl, and transformed into E. coli by electroporation.Clones were confirmed by sequencing, resulting in plasmid pMEV3-ΔxylA(FIG. 1A).

The pMEV3-ΔxylA plasmid was then transformed into strain E. coli strainSEL700 (MG1655 Δ^(r), Δ(recA-srl)306,srl-301::Tn10-84(Tets), [F′proAB,lacl^(q), ZΔM15, Tn10 (Tet^(r))] pJC859). Single-crossover events wereselected for by plating on LB agar plates containing kanamycin (finalconcentration, about 50 μg/ml). After confirmation of integration ofpMEV3-ΔxylA on the chromosome, a second crossover event was selected forby growth on LB agar media containing sucrose (FIG. 2). Coloniesdisplaying resistance to kanamycin and the ability to grow on sucrosewere screened both by PCR characterization with primers flanking thexylA gene to confirm gene deletion and by growth on a modified MacConkeymedia (ABD media), comprised of: MacConkey Agar Base (Difco™ #281810)(approximate formula per liter: Pancreatic Digest of Gelatin (17.0 g)Peptones (meat and casein) (3.0 g), Bile Salts No. 3 (1.5 g), SodiumChloride (5.0 g), Agar (13.5 g), Neutral Red (0.03 g), Crystal Violet(1.0 g, Xylose (30.0 g) and Kanamycin (50 mg). The ABD media containedneutral red, a pH indicator that turns red at a pH<6.8. Colonies ofmutants lacking xylA appeared white on this media while colonies withrestored xylose metabolism ability appeared red in color due to thefermentation of xylose to xylulose, which lowered the pH of the mediasurrounding those colonies.

Following the successful deletion of xylA, the resulting strain wascured of pJC859 by the following method: The xylA deletion strain wasgrown for about 24 hours in LB media containing tetracycline at a finalconcentration, about 20 μg/ml, at around 37° C. The next day the cellswere sub-cultured (1:100 dilution) into LB tetracycline (at the sameconcentration) media and incubated at about three different temperatures(30, 37, and 42° C.). Cells were passaged the same way as above forabout two more days. Dilutions of the resulting cultures were plated onLB plates to isolate single colonies. Colonies were replica plated ontoLB agar plates with and without Carbenicillin (at about 100 μg/ml, finalconcentration). Carbenicillin resistant colonies were deemed to stillcontain vector pJC859 whereas carbenicillin sensitive colonies werecured of pJC859, restoring the recA genotype of strain SEL700. Thisstrain, SEL700 ΔxylA, was used for the ABD screening.

The ABD screening method was verified by creating a positive controlstrain by PCR amplification of the xylA gene from E. coli K12 andcloning into the PCR-BluntII TOPO vector (Invitrogen, Carlsbad, Calif.)using standard procedures. This vector (PCR-BluntII-TOPO-xylA, FIG. 1B)was then transformed into the screening strain (SEL700 ΔxylA).Complementation of the xylose phenotype was verified by growth oftransformants on ABD media and appearance of red halos indicating xyloseutilization.

The libraries were screened for XI activity by infecting strain SEL700ΔxylA with the excised phagemid libraries. Infected cells were platedonto ABD media and only colonies with red “halos” (indicating xylosefermentation), were carried forward. Positives were purified to singlecolonies, and regrown on ABD media to confirm phenotype.

5.3 Example 2 Sequence-Based Discovery for Xylose Isomerases

Libraries used for sequence-based discovery (“SBD”) were in the formatof genomic DNA (gDNA) extractions. These libraries were constructed asdescribed in U.S. Pat. No. 6,280,926. Sources for these libraries weresamples collected from the guts of deceased herbivores.

XI genes often exist in conserved gene clusters (Dodd et al., 2011,Molecular Microbiol. 79:292-304). In order to obtain full length XI genesequences from metagenomic samples, primers were designed to bothupstream and downstream conserved DNA sequences found in severalBacteroides species, typically xylulose kinase and xylose permease,respectively. These flanking DNA sequences were obtained from publicdatabases. Sample genomic DNA was extracted from eleven different animalrumen samples. Left flanking consensus primer has the sequence5′-GCIGCICARGARGGNATYGTVTT-3′ (SEQ ID NO:177) (this primer codes for theamino acid motif AAQEGIV(F) (SEQ ID NO:178)). Right flanking consensusprimer has the sequence 5′-GCDATYTCNGCRATRTACATSGG-3′ (SEQ ID NO:179)(this primer codes for the amino acid motif PMYIAEIA (SEQ ID NO:180)).PCR reactions were carried out using touchdown cycling conditions, andhot start Platinum® Taq DNA polymerase (Invitrogen, Carlsbad, Calif.).PCR products of expected size were purified and subcloned into pCR4-TOPOvector system (Invitrogen, Carlsbad, Calif.). Positive colonies from theTOPO-based PCR libraries were transformed into TOP10 (Invitrogen,Carlsbad, Calif.) and the transformants grown on LB agar plates withkanamycin (about 25 μg/ml final concentration). Resistant colonies werepicked and inoculated into 2 columns each of a 96-deep well plate inabout 1.2 ml LB kanamycin (25 μg/ml final concentration) media per well.Cultures were grown overnight at about 30° C. The next day plasmids werepurified and inserts sequenced. Sequence analysis revealed multiple fulllength XI genes. Identification of putative ORFs was done by identifyingstart and stop codons for the longest protein coding region, andsubsequent manual curation based on homology to published xyloseisomerase DNA sequences.

5.4 Example 3 XI Sequence Analysis

Plasmids from both ABD and SBD screens were purified and vector insertswere sequenced using an ABI 3730xl DNA Analyzer and ABI BigDye® v3.1cycle sequencing chemistry. Identification of putative ORFs was done byidentifying start and stop codons for the longest protein coding region,and subsequent manual curation based on homology to published xyloseisomerase DNA sequences. The XI ORF identified are set forth in Table 2below, which indicates the sequences and source organism classificationfor each XI determined from either the ABD or SBD libraries as well astheir assigned sequence identifiers. The putative catalytic domains(based on sequence alignments with other XIs) are underlined.

TABLE 2 Type SEQ of ID Clone No. Class of organism Sequence NO: Sequence1754MI2_001 Bacteroidales DNA 1ATGGCAGTTAAAGAATATTTCCCGGAGATAGGCAAGATCGCCTTTGAAGGAAAGGAGTCCAAGAACCCTATGGCATTCCACTACTACAATCCAGAGCAGGTAGTAGCCGGAAAGAAAATGAAAGATTGGTTCAAGTTCGCTATGGCATGGTGGCACACCCTCTGCGCTGAAGGTGGCGACCAGTTCGGTCCTGGTACCAAGAAATTCCCTTGGAACACAGGTGCAACTGCACTCGAAAGAGCAAAGAACAAAATGGACGCAGGTTTCGAGATCATGAGCAAGCTCGGTATCGAGTATTTCTGCTTCCACGATGTTGACCTTATCGACGAGGCTGACACTGTTGAAGAGTACGAGGCTAACATGAAGGCTATCACAGCTTACGCAAAGGAGAAAATGGCCGCTACTGGCATCAAACTCCTCTGGGGAACAGCCAATGTATTCGGCAACAAGAGATATATGAACGGCGCTTCTACCAACCCTGACTTCAACGTGGCTGCACGCGCTATGCTCCAGATCAAGAACGCTATCGACGCAACTATCGCTCTCGGTGGTGACTGCTATGTATTCTGGGGCGGCCGTGAGGGTTACATGAGCCTTCTCAACACCGATATGAAGAGAGAGAAAGAGCACATGGCTACCATGCTTACCATGGCACGCGACTATGCTCGTTCTAAGGGCTTCAAGGGTACCTTCCTTATCGAGCCTAAGCCAATGGAGCCGATGAAGCACCAGTACGATGTCGATACTGAGACTGTCGTAGGTTTCCTCCGCGCCCATGGTCTTGACAAGGACTTCAAGGTAAACATCGAGGTTAACCACGCTACTCTCGCAGGCCACACCTTCGAGCACGAGCTCCAGTGCGCCGTTGACGCAGGCATGCTCGGAAGCATCGACGCCAACCGTGGTGACTACCAGAACGGCTGGGATACCGACCAGTTCCCTATCGACCTCTATGAGCTCGTACAGGCTATGATGGTTATCATCAAGGGCGGCGGTCTCGTCGGCGGTACCAACTTCGACGCCAAGACCCGTCGTAACTCAACAGACCTCGAGGATATCTTCATCGCTCATGTATCCGGCATGGATGTCATGGCACGCGCTCTCCTCATCGCTGCTGACCTTCTCGAGAAATCTCCTATTCCTGCAATGGTCAAGGAGCGTTACGCTTCCTACGACTCAGGCATGGGCAAGGACTTCGAGAACGGCAAGCTTACTCTCGAGCAGGTTGTCGATTTCGCAAGAAAGAACGGCGAGCCTAAGAGCACCAGCGGAAAGCAGGAGCTCTACGAGTCTATCGTCAATCTCTACATCTAA 1754MI2_001Bacteroidales Amino 2MAVKEYFPEIGKIAFEGKESKNPMAFHYYNPEQVVAGKKMKDWFKFAMAWWHTLCAEGGD AcidQFGPGTKKFPWNTGATALERAKNKMDAGFEIMSKLGIEYFCFHDVDLIDEADTVEEYEANMKAITAYAKEKMAATGIKLLWGTANVFGNKRYMNGASTNPDFNVAARAMLQIKNAIDATIALGGDCYVFWGGREGYMSLLNTDMKREKEHMATMLTMARDYARSKGFKGTFLIEPKPMEPMKHQYDVDTETVVGFLRAHGLDKDFKVNIEVNHATLAGHTFEHELQCAVDAGMLGSIDANRGDYQNGWDTDQFPIDLYELVQAMMVIIKGGGLVGGTNFDAKTRRNSTDLEDIFIAHVSGMDVMARALLIAADLLEKSPIPAMVKERYASYDSGMGKDFENGKLTLEQVVDFARKNGEPKSTSGKQELYESIVNLYI 5586MI6_004 Bacteroidales DNA 3ATGGCAAACAAAGAGTACTTCCCGGAGATCGGGAAAATCAAATTCGAAGGCAAGGATTCCAAGAACCCGCTTGCATTCCATTATTACAATCCTGAGCAGGTCGTCTGCGGCAAGCCGATGAAGGACTGGCTCAAGTTCGCTATGGCATGGTGGCACACCCTCTGCGCAGAGGGTAGCGACCAGTTCGGCGGACCCACCAAGTCATTCCCTTGGAACAAAGCTTCGGATCCCATCGCAAAGGCCAAGCAGAAAGTCGACGCCGGTTTCGAGATCATGCAGAAGCTCGGTATCGGATACTATTGCTTCCACGATGTAGACCTCATCGACGAGCCCGCCACCATCGAGGAGTATGAGGCCGATCTCAAGGAGATCGTCGCTTACCTCAAGGAGAAGCAGGCCCAGACCGGCATCAAGCTCCTTTGGGGCACCGCCAACGTCTTCGGTCACAAGCGGTACATGAACGGCGCCTCCACCAACCCTGATTTCGACGTCGCAGCCCGCGCCATGGTCCAGATCAAGAACGCCATGGACGCCACCATCGAGCTCGGCGGCGAGTGCTATGTCTTCTGGGGCGGCCGCGAGGGCTACATGAGCCTCCTCAACACCGACATGAAGCGTGAGAAGCAGCATATGGCCACCATGCTCGGCATGGCCCGCGACTATGCACGCGGCAAGGGCTTCAAGGGCACCTTCCTCATCGAGCCCAAGCCCATGGAGCCGACCAAGCACCAGTATGACGTCGACACCGAGACCGTCATCGGTTTCCTCCGTGCCAACGGTCTTGACAAGGACTTCAAGGTCAACATCGAGGTCAATCACGCCACCCTCGCCGGCCACACCTTCGAGCATGAGCTCCAGTGCGCCGCCGATGCCGGTCTCCTCGGATCCATCGACGCCAACCGCGGCGACTATCAGAACGGCTGGGATACCGACCAGTTCCCGATCGACCTCTATGAGCTCACCCAGGCCATGATGGTCATCCTCAAGAATGGCGGCCTCGTCGGCGGTACCAACTTCGACGCCAAGACCCGTCGCAACTCCACCGACCTGGACGACATCATCATCGCCCACGTCAGCGGTATGGACATCATGGCACGCGCACTCCTCGTCGCTGCCGACGTCCTCACCAAGTCCGAGCTTCCCAAGATGCTCAAGGAGCGTTACGCTTCCTTCGACTCCGGCAAGGGCAAGGAGTTCGAAGAGGGCAAGCTCACTCTCGAGCAGGTCGTAGAGTACGCCAAGACCAAGGGCGAGCCCAAGGCCACCAGCGGCAAGCAGGAGCTCTACGAGACCATCGTCAACATGTACATCTAA 5586MI6_004Bacteroidales Amino 4MANKEYFPEIGKIKFEGKDSKNPLAFHYYNPEQVVCGKPMKDWLKFAMAWWHTLCAEGSD AcidQFGGPTKSFPWNKASDPIAKAKQKVDAGFEIMQKLGIGYYCFHDVDLIDEPATIEEYEADLKEIVAYLKEKQAQTGIKLLWGTANVFGHKRYMNGASTNPDFDVAARAMVQIKNAMDATIELGGECYVFWGGREGYMSLLNTDMKREKQHMATMLGMARDYARGKGFKGTFLIEPKPMEPTKHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELQCAADAGLLGSIDANRGDYQNGWDTDQFPIDLYELTQAMMVILKNGGLVGGTNFDAKTRRNSTDLDDIIIAHVSGMDIMARALLVAADVLTKSELPKMLKERYASFDSGKGKEFEEGKLTLEQVVEYAKTKGEPKATSGKQELYETIVNMYI 5749MI1_003 Bacteroidales DNA 5ATGAATTTTTATAAAGGCGAAAAAGAATTCTTCCCCGGAATAGGAAAGATTCAGTTTGAAGGACGCGAGTCAAAGAACCCGATGGCGTTTCATTATTATGACGAAAACAAGGTGGTGATGGGTAAAACACTGAAGGATCATCTTCGTTTTGCAATGGCTTACTGGCATACGCTTTGTGCCGAAGGGGGCGACCAGTTTGGCGGTGGTACGAAAACATTCCCCTGGAATGCTGCTGCCGACCCGATCAGCCGTGCCAAATATAAGATGGATGCAGCGTTCGAGTTTATGACAAAATGCAGCATCCCTTATTACTGTTTCCATGATGTGGACGTGGTGGACGAAGCTCCCACGCTGGCTCAGTTTGAAAAAGACCTTCATACGATGGTAGGCCATGCCAAAGGGCTTCAGCAGGCAACCGGAAAAAAACTGTTATGGTCTACTGCCAACGTGTTCAGCAACAAACGCTATATGAACGGGGCTGCCACTAATCCTGACTTCTCGGCCGTGGCTTGTGCCGGTACGCAGATCAAGAATGCGATCGATGCCTGTATCGCGCTGGACGGTGAAAACTATGTGTTCTGGGGCGGACGTGAAGGATATATGGGCTTGCTCAATACCGATATGAAACGCGAAAAAGACCATCTGGCCATGATGCTGACGATGGCACGCGACTATGGCCGCAAGAACGGTTTCAAAGGTACTTTCCTGATCGAGCCGAAACCGATGGAACCGACCAAGCATCAATATGATGTCGACTCGGAAACTGTAATCGGCTTCCTACGTCATTATGGCCTGGATAAAGACTTCGCCCTGAATATCGAAGTAAATCATGCAACCCTGGCCGGACATACGTTCGAGCACGAATTGCAGGCTGCTGTCGATGCCGGTATGCTGTGCAGTATCGATGCCAACCGTGGTGACTACCAGAATGGCTGGGATACCGACCAATTCCCGATGGACATCTACGAACTGACTCAGGCTTGGCTGGTCATTCTGCAAGGTGGTGGTCTGACAACCGGCGGAACGAACTTCGATGCCAAGACCCGCCGCAACTCGACCGACCTGGACGATATCTTCCTGGCTCATATAGGTGGTATGGATGCGTTTGCCCGTGCCCTGATCACGGCTGCTGCCATCCTTGAAAACTCCGATTACACGAAGATGCGTGCCGAACGTTACACCAGCTTCGATGGTGGCGAAGGCAAAGCGTTTGAAGACGGTAAACTTTCTCTGGAAGACCTGCGTACGATCGCTCTCCGCGACGGAGAACCGAAGATGGTCAGCGGCAAACAGGAATTATATGAGATGATTCTCAATTTA TACATATAA5749MI1_003 Bacteroidales Amino 6MNFYKGEKEFFPGIGKIQFEGRESKNPMAFHYYDENKVVMGKTLKDHLRFAMAYWHTLCA AcidEGGDQFGGGTKTFPWNAAADPISRAKYKMDAAFEFMTKCSIPYYCFHDVDVVDEAPTLAQFEKDLHTMVGHAKGLQQATGKKLLWSTANVFSNKRYMNGAATNPDFSAVACAGTQIKNAIDACIALDGENYVFWGGREGYMGLLNTDMKREKDHLAMMLTMARDYGRKNGFKGTFLIEPKPMEPTKHQYDVDSETVIGFLRHYGLDKDFALNIEVNHATLAGHTFEHELQAAVDAGMLCSIDANRGDYQNGWDTDQFPMDIYELTQAWLVILQGGGLTTGGTNFDAKTRRNSTDLDDIFLAHIGGMDAFARALITAAAILENSDYTKMRAERYTSFDGGEGKAFEDGKLSLEDLRTIALRDGEPKMVSGKQELYEMILNLYI 5750MI1_003 Bacteroidales DNA 7ATGAATTACTTTAAAGGTGAGAAAGAGTTCTTCCCGGGAATCGGGAAAATAGAGTTTGAAGGACGTGAATCGAAGAATCCGATGGCTTTTCATTACTATGACGAGAACAAGGTTGTCATGGGGAAGACCTTGAAGGACCATCTGCGTTTTGCGATGGCTTATTGGCATACGCTGTGTGCGGAAGGCGCCGACCAGTTCGGCGGCGGGACGAAGGCATTTCCCTGGAATACCGGGGCGGATCGTATTTCCCGTGCCAAGTATAAGATGGATGCTGCTTTTGAGTTTATGACGAAATGTAACATCCCGTACTATTGTTTCCATGATGTGGATGTGGTGGATGAAGCTCCGACACTGGCCGAATTTGAAAAAGACTTGCATACGATGGTCGAATATGCCAAGCAGCATCAGGAGGCAACCGGGAAAAAACTGTTGTGGTCTACCGCCAATGTGTTCAGCAATAAACGTTATATGAACGGGGCTGCCACAAATCCGTATTTCCCTGCTGTCGCTTGTGCGGGTACGCAGATCAAGAATGCTATCGACGCTTGTATTGCCCTGGGCGGCGAAAACTATGTGTTCTGGGGCGGTCGTGAAGGGTATATGAGCTTGTTGAACACCAATATGAAACGCGAAAAGGAACATCTCGCCATGATGTTGACGATGGCTCGCGATTATGCGCGTAAGAACGGCTTCAAAGGTACTTTCCTGGTAGAGCCTAAACCGATGGAACCGACCAAACATCAGTATGATGTGGACACAGAAACTGTTATCGGCTTCCTGCGTCATTACGGCCTTGACAAGGACTTTGCCATCAACATCGAAGTGAATCATGCTACATTGGCTGGACATACATTCGAACATGAGCTTCAGGCGGCTGCCGATGCCGGTATGCTGTGCAGCATCGACGCCAACCGCGGCGATTACCAGAATGGTTGGGACACGGATCAGTTCCCGGTCGACATCTACGAACTGACACAGGCGTGGCTGGTTATCCTCGAAGCGGGTGGCCTGACTACCGGTGGTACGAACTTCGACGCCAAGACGCGCCGCAACTCGACTGACCTGGACGATATCTTCCTGGCACACATCGGTGGTATGGATTCGTTTGCCCGTGCTTTGATGGCGGCTGCCGATATATTGGAACACTCCGATTACAAAAAGATGCGTGCCGAACGTTATGCCAGCTTCGATCAAGGCGACGGCAAGAAGTTCGAAGATGGTAAACTCCTTCTCGAGGACCTCCGCACCATCGCTCTTGCCTCCGGCGAACCGAAGCAAATCAGCGGGAAACAGGAATTGTATGAAATGATTATCAACCAG TACATTTAA5750MI1_003 Bacteroidales Amino 8MNYFKGEKEFFPGIGKIEFEGRESKNPMAFHYYDENKVVMGKTLKDHLRFAMAYWHTLCA AcidEGADQFGGGTKAFPWNTGADRISRAKYKMDAAFEFMTKCNIPYYCFHDVDVVDEAPTLAEFEKDLHTMVEYAKQHQEATGKKLLWSTANVFSNKRYMNGAATNPYFPAVACAGTQIKNAIDACIALGGENYVFWGGREGYMSLLNTNMKREKEHLAMMLTMARDYARKNGFKGTFLVEPKPMEPTKHQYDVDTETVIGFLRHYGLDKDFAINIEVNHATLAGHTFEHELQAAADAGMLCSIDANRGDYQNGWDTDQFPVDIYELTQAWLVILEAGGLTTGGTNFDAKTRRNSTDLDDIFLAHIGGMDSFARALMAAADILEHSDYKKMRAERYASFDQGDGKKFEDGKLLLEDLRTIALASGEPKQISGKQELYEMIINQYI 5750MI2_003 Bacteroidales DNA 9ATGAATTATTTTAAAGGTGAAAAAGAGTTTTTCCCTGGAATCGGGAAAATAGAGTTTGAAGGACGTGAGTCGAAGAATCCGATGGCTTTTCATTATTATGATGAAAACAAGGTCGTAATGGGCAAGACCTTGAAAGATCACCTCCGCTTTGCAATGGCTTACTGGCATACGTTGTGCGCGGAAGGCGCAGACCAGTTTGGCGGTGGCACAAAATCATTCCCCTGGAATACCGCAGCGGATCGTATTTCCCGCGCTAAATATAAAATGGATGCTGCTTTCGAGTTTATGACCAAGTGCAGTATCCCGTACTATTGTTTCCATGATGTGGACGTGGTGGACGAAGCTCCGGCACTGGCCGAATTTGAAAAGGACCTGCATACGATGGTGGGATTCGCCAAACAACACCAGGAAGCAACCGGAAAGAAACTGTTGTGGTCTACAGCCAATGTATTCGGGCATAAACGTTATATGAACGGAGCGGCTACCAATCCTTATTTCCCGGCTGTCGCTTGTGCCGGTACGCAGATCAAGAATGCAATCGACGCCTGTATCGAGCTGGGTGGAGAGAACTATGTATTCTGGGGCGGACGCGAAGGCTACATGAGCCTGCTGAACACCAATATGAAACGTGAAAAGGATCATTTGGCCATGATGCTGACAATGGCACGCGATTATGCCCGCAAGAATGGTTTCAAGGGTACTTTCCTGGTGGAATCTAAGCCGATGGAACCGACCAAACATCAGTATGACGCAGATACGGAAACCGTGATCGGCTTCCTGCGCCACTATGGCCTCGACAAGGATTTCGCTATCAACATTGAAGTGAACCATGCTACATTGGCCGGCCATACATTCGAACATGAACTTCAGGCTGCTGCCGATGCCGGTATGCTGTGCAGCATCGATGCAAATAGAGGCGACTATCAGAATGGTTGGGATACGGATCAGTTCCCCGTAGACATTTACGAACTGACACAGGCCTGGCTGGTTATCCTGGAAGCGGGCGGACTGACAACCGGAGGTACGAACTTCGATGCGAAGACCCGTCGTAACTCGACTGACCTCGACGATATCTTCCTGGCCCATATCGGCGGTATGGATTCGTTTGCACGTGCCTTGATGGCAGCTGCCGATATCCTGGAACATTCTGATTACAAGAAGATGCGTGCCGAACGTTACGCCAGCTTCGACCAGGGCGACGGCAAGAAGTTCGAAGACGGCAAACTCCTTCTCGAAGACCTGCGCACAATTGCCCTTGCCGGCGACGAACCGAAGCAGATCAGCGGCAAGCAGGAGTTGTATGAGATGATTATCAATCAG TATATTTAA5750MI2_003 Bacteroidales Amino 10MNYFKGEKEFFPGIGKIEFEGRESKNPMAFHYYDENKVVMGKTLKDHLRFAMAYWHTLCA AcidEGADQFGGGTKSFPWNTAADRISRAKYKMDAAFEFMTKCSIPYYCFHDVDVVDEAPALAEFEKDLHTMVGFAKQHQEATGKKLLWSTANVFGHKRYMNGAATNPYFPAVACAGTQIKNAIDACIELGGENYVFWGGREGYMSLLNTNMKREKDHLAMMLTMARDYARKNGFKGTFLVESKPMEPTKHQYDADTETVIGFLRHYGLDKDFAINIEVNHATLAGHTFEHELQAAADAGMLCSIDANRGDYQNGWDTDQFPVDIYELTQAWLVILEAGGLTTGGTNFDAKTRRNSTDLDDIFLAHIGGMDSFARALMAAADILEHSDYKKMRAERYASFDQGDGKKFEDGKLLLEDLRTIALAGDEPKQISGKQELYEMIINQYI 5586MI5_004 Bacteroides DNA 11ATGAAACAGTATTTCCCGAACATCTCCGCCATCAAGTTTGAGGGCGTCGAGAGCAAGAATCCCCTGGCTTACCGCTACTACGACCGCGACCGCGTCGTCATGGGTAAGAAGATGAGCGAATGGTTTAAGTTCGCTATGTGCTGGTGGCACACCCTCTGCGCCGAGGGCTCCGATCAGTTCGGTCCCGGCACAAAGACCTTCCCCTGGAACGCCGCCGCCGACCCCGTGCAGGCTGCCAAGGACAAGGCCGACGCTGGCTTCGAGATCATGCAGAAACTCGGCATCGAGTACTACTGCTTCCACGACGTTGACCTCGTGGCCGAGGCTCCCGACGTGGAGACCTACGAGAAGAACCTCAAGGAGATCGTGGCTTATCTCAAGCAGAAACAGGCTGAGACGGGCATCAAGCTGCTCTGGGGCACTGCCAACGTCTTCGGACACAAGCGCTACATGAACGGAGCCTCCACGAACCCCGACTTCGATGTCGTGGCACGCGCTATCGTGCAGATCAAGAACGCCATCGATGCTACCATCGAGCTGGGCGGCACCAACTACGTCTTCTGGGGCGGTCGCGAAGGCTACATGAGCCTGCTCAACACCGATATGAAGCGCGAGAAGGAGCACATGGCTACGATGTTGACGATGGCACGCGACTATGCCCGTTCTAAGGGATTCAAGGGCACGTTCCTCATCGAACCCAAACCCATGGAACCCACGAAGCATCAGTACGATGCGGACACCGAGACGGTCATCGGATTCCTCCGTGCTCATGGTCTCGACAAGGATTTCAAGGTCAACATCGAGGTCAACCACGCCACGCTGGCCGGACACACGTTCGAGCATGAGCTGGCCTGCGCCGTAGACGCCGATATGCTCGGCAGCATCGATGCCAATCGCGGCGACTATCAGAACGGATGGGACACCGACCAGTTCCCCATCGACCACTACGAACTCACGCAGGCTATGCTGCAGATCATCCGCAACGGAGGTTTCAAGGACGGTGGCACCAATTTTGACGCTAAGACGCGCCGCAACAGCACCGACCTCGAGGATATCTTCATCGCTCACGTAGCAGCCATGGACGCCATGGCCCACGCCCTGTTGTCGGCTGCCGATATCATCGAGAAGTCGCCCATCTGCACGATGGTCAAGGAGCGTTACGCCAGCTTCGATGCCGGCGAAGGCAAGCGCTTCGAAGAAGGCAAGATGACCCTCGAGGAAGCCTACGAGTATGGCAAGAAGGTCGGGGAGCCCAAGCAGACCAGCGGAAAGCAGGAGCTCTACGAAGCCATTGTCAATATGTATTGA 5586MI5_004 BacteroidesAmino 12 MKQYFPNISAIKFEGVESKNPLAYRYYDRDRVVMGKKMSEWFKFAMCWWHTLCAEGSDQFAcid GPGTKTFPWNAAADPVQAAKDKADAGFEIMQKLGIEYYCFHDVDLVAEAPDVETYEKNLKEIVAYLKQKQAETGIKLLWGTANVFGHKRYMNGASTNPDFDVVARAIVQIKNAIDATIELGGTNYVFWGGREGYMSLLNTDMKREKEHMATMLTMARDYARSKGFKGTFLIEPKPMEPTKHQYDADTETVIGFLRAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDADMLGSIDANRGDYQNGWDTDQFPIDHYELTQAMLQIIRNGGFKDGGTNFDAKTRRNSTDLEDIFIAHVAAMDAMAHALLSAADIIEKSPICTMVKERYASFDAGEGKRFEEGKMTLEEAYEYGKKVGEPKQTSGKQELYEAIVNMY 5586MI202_004 Bacteroides DNA 13ATGGCAACAAAAGAGTATTTTCCCGGAATAGGAAAGATTAAATTCGAAGGTAAAGAGAGTATGAACCCGATGGCATATCGTTACTACGATGCTGAGAAGGTAATCATGGGTAAGAAGATGAAAGATTGGTTGAAGTTTGCTATGGCTTGGTGGCACACTCTCTGCGCAGAAGGTGGTGACCAATTCGGTGGCGGAACGAAACAATTCCCTTGGAATGGTGACTCTGACGCTTTGCAAGCAGCTAAAAATAAATTGGATGCAGGTTTCGAATTCATGCAGAAGATGGGTATCGAATACTATTGCTTCCACGATGTAGACCTGATTTCTGAAGGTGCAAGCATCGAAGAATACGAAGCTAACTTGAAAGCTATCGTAGCTTATGCAAAAGAAAAACAGGCTGAAACTGGTATCAAGCTGTTGTGGGGTACTGCTAACGTATTCGGTCATGCACGTTATATGAACGGTGCTGCTACCAATCCTGATTTCGACGTTGTAGCACGCGCTGCTGTTCAGATCAAGAACGCTATTGACGCTACTATCGAACTGGGTGGTTCAAACTATGTATTCTGGGGCGGTCGCGAAGGTTACATGTCTTTGCTGAACACTGACCAGAAACGTGAAAAAGAACACCTTGCAAAGATGTTGACTATCGCTCGTGACTATGCACGTGCTCGTGGCTTCAAAGGTACTTTCCTGATTGAGCCGAAACCGATGGAACCGACAAAACATCAGTATGATGTAGATACTGAAACAGTTATCGGCTTCCTGAAAGCTCACGGTTTGGATAAGGATTTCAAAGTAAACATCGAGGTTAATCACGCAACTTTGGCTGGCCATACTTTCGAACACGAACTGGCTGTAGCTGTTGACAACGGCATGTTAGGTTCTATCGACGCTAACCGTGGTGACTACCAGAACGGTTGGGATACTGACCAATTCCCTATCGATAACTACGAACTGACTCAAGCTATGATGCAGATCATCCGCAACGGTGGTTTGGGTAATGGCGGTACTAACTTCGACGCTAAGACCCGTCGTAACTCTACCGACCTGGAAGATATCTTCATCGCTCACATTGCAGGTATGGATGCTATGGCACGTGCTCTGGAAAGTGCAGCTAAATTACTGGAAGAATCTCCTTATAAGAAAATGTTGGCTGATCGTTACGCATCATTCGACGGTGGCAAGGGTAAGGAATTCGAAGAAGGCAAATTGTCTTTGGAAGATGTTGTAGCTTATGCGAAAGCTAACGGCGAACCGAAGCAAACCAGCGGCAAGCAAGAATTGTATGAAGCAATCGTGAATATGTATTGCTAA 5586MI202_004Bacteroides Amino 14MATKEYFPGIGKIKFEGKESMNPMAYRYYDAEKVIMGKKMKDWLKFAMAWWHTLCAEGGD AcidQFGGGTKQFPWNGDSDALQAAKNKLDAGFEFMQKMGIEYYCFHDVDLISEGASIEEYEANLKAIVAYAKEKQAETGIKLLWGTANVFGHARYMNGAATNPDFDVVARAAVQIKNAIDATIELGGSNYVFWGGREGYMSLLNTDQKREKEHLAKMLTIARDYARARGFKGTFLIEPKPMEPTKHQYDVDTETVIGFLKAHGLDKDFKVNIEVNHATLAGHTFEHELAVAVDNGMLGSIDANRGDYQNGWDTDQFPIDNYELTQAMMQIIRNGGLGNGGTNFDAKTRRNSTDLEDIFIAHIAGMDAMARALESAAKLLEESPYKKMLADRYASFDGGKGKEFEEGKLSLEDVVAYAKANGEPKQTSGKQELYEAIVNMYC 5586MI211_003 Bacteroides DNA 15ATGGCAAAAGAGTATTTTCCTGGCGTGAAAAAAATCCAGTTCGAGGGTAAGGACAGTAAGAATCCAATGGCTTACCGTTATTATGATGCAGAGAAGGTCATCATGGGTAAGAAGATGAAGGATTGGTTGAAGTTCGCTATGGCTTGGTGGCACACTTTGTGCGCTGAGGGCGCAGACCAGTTCGGTGGCGGTACTAAGACTTTCCCTTGGAACGAAGGTGCAAACGCTTTGGAAGTTGCTAAGAATAAGGCTGATGCTGGTTTCGAGATTATGGAGAAGCTTGGCATCGAGTACTACTGTTTCCACGATGTAGACCTCGTTGAGGAGGCTGCAACTATCGAGGAGTATGAGGCTAACATGAAGGCTATCGTTGCTTATCTTAAGGAGAAGCAGGCTGCTACTGGCAAGAAGCTTCTTTGGGGTACTGCTAACGTATTCGGCAACAAGCGCTATATGAACGGTGCTTCTACAAACCCTGACTTCGACGTTGTTGCTCGCGCTTGTGTTCAGATTAAGAACGCTATCGACGCTACTATCGAACTTGGTGGTACAAACTACGTATTCTGGGGTGGCCGCGAGGGTTATATGAGCCTTCTTAACACAGATATGAAGCGTGAGAAGGAGCACATGGCAACTATGCTTACTAAGGCTCGCGACTACGCTCGTTCAAAGGGCTTTACTGGTACATTCCTTATCGAGCCAAAGCCAATGGAACCATCAAAGCATCAGTATGATGTTGATACTGAGACTGTTTGTGGTTTCTTGAGGGCTCACGGTCTTGACAAGGACTTCAAGGTAAACATCGAGGTTAACCACGCTACTTTGGCTGGTCACACATTCGAGCACGAGTTGGCTGCTGCTGTTGATAACGGTATGCTTGGCTCTATCGACGCTAACCGCGGTGACTACCAGAACGGTTGGGATACTGACCAGTTCCCTATCGACAACTTCGAGCTTATTCAGGCTATGATGCAGATTATCCGCAACGGTGGTCTTGGCAACGGTGGTACAAACTTCGACGCTAAGACTCGTCGTAACTCAACTGACCTTGAGGATATCTTCATCGCACACATCGCTGGTATGGATGCAATGGCTCGCGCTCTTGAGAACGCAGCAGACCTTTTGGAGAACTCTCCAATCAAGAAGATGGTTGCTGAGCGTTACGCTTCATTCGACAGCGGCAAGGGTAAGGAGTTCGAGGAAGGCAAGTTGAGCCTTGGGGACATCGTTGCTTATGCTAAGCAGAACGGTGAGCCTAAGCAGACAAGCGGTAAGCAGGAGCTTTACGAGGCTATCGTAAACATGTACTGCTAA 5586MI211_003Bacteroides Amino 16MAKEYFPGVKKIQFEGKDSKNPMAYRYYDAEKVIMGKKMKDWLKFAMAWWHTLCAEGADQ AcidFGGGTKTFPWNEGANALEVAKNKADAGFEIMEKLGIEYYCFHDVDLVEEAATIEEYEANMKAIVAYLKEKQAATGKKLLWGTANVFGNKRYMNGASTNPDFDVVARACVQIKNAIDATIELGGTNYVFWGGREGYMSLLNTDMKREKEHMATMLTKARDYARSKGFTGTFLIEPKPMEPSKHQYDVDTETVCGFLRAHGLDKDFKVNIEVNHATLAGHTFEHELAAAVDNGMLGSIDANRGDYQNGWDTDQFPIDNFELIQAMMQIIRNGGLGNGGTNFDAKTRRNSTDLEDIFIAHIAGMDAMARALENAADLLENSPIKKMVAERYASFDSGKGKEFEEGKLSLGDIVAYAKQNGEPKQTSGKQELYEAIVNMYC 5606MI1_005 Bacteroides DNA 17ATGGCGACAAAAGAATACTTTCCCGGAATAGGGAAAATCAAGTTTGAGGGTGTGAATAGCTATAATCCGCTGGCATACAGATATTACGATGCCGAGCGCATAGTCCTTGGCAAGCCGATGAAGGAGTGGCTCAAGTTTGCCATGGCATGGTGGCACACACTCTGCGCAGAGGGTGGCGACCAGTTTGGCGGCGGTACGAAGAATTTTCCCTGGAATGGAGATCCCGATCCGGTACAGGCCGCAAAAAACAAAGTAGACGCCGGCTTCGAATTCATGACCAAGATGGGAATAGAGTATTTCTGTTTCCACGACGTGGATCTCGTCAGCGAGGCAGCAACCATCGAGGAGTATGAGGCCAACCTGAAGGAAGTGGTGGGCTACATCAAGGAAAAGCAGGCCGAGACGGGGATCAAAAACCTCTGGGGCACTGCCAACGTGTTCAGCCACGCGCGCTACATGAACGGAGCCGCCACCAACCCCGACTTCGATGTAGTGGCCCGCGCAGCCGTGCAGATCAAGAATGCTATCGACGCCACGATAGCCTTAGGTGGCACCAACTACGTGTTCTGGGGTGGCCGTGAAGGTTACATGAGCCTGCTCAACACCGACCAGAAGCGCGAGAAGGAGCATCTGGCAATGATGCTCCGCATGGCCCGCGACTATGCGCGTGCAAAAGGCTTCACCGGCACCTTCCTTATCGAGCCCAAGCCGATGGAGCCCACCAAGCACCAGTATGATGTAGACACCGAGACTGTGATAGGCTTCCTCCGTGCCCACGGCCTCGACAAGGACTTCAAGGTCAACATAGAGGTGAACCACGCCACCCTGGCCGGCCATACCTTCGAGCATGAGCTGGCAGTGGCCGTGGACAACGGTATGCTCGGCAGCATCGACGCCAACCGCGGTGACTACCAGAACGGCTGGGATACCGACCAGTTCCCCATCGACAACTACGAGCTGACCCAGGCCATGATGCAGATAATACGCAACGGCGGCTTCGGCAACGGCGGATGCAACTTCGACGCCAAGACACGCCGCAACTCCACCGACCTGGAGGATATCTTCATAGCCCACATAGCAGGCATGGACGCCATGGCCCGCGCCCTGCTCAGCGCAGCAGAAGTGCTGGAGAAATCGCCCTACAGGAAGATGCTCGCCGAGCGCTACGCACCGTTTGATGCCGGCCAGGGAAAGGCATTTGAAGAGGGCGCAATGTCGCTCACCGACCTTGTGGAGTATGCCAAGGAGCATGGCGAGCCCACACAGACTTCCGGCAAGCAGGAACTCTATGAGGCAATCGTCAATATGTATTGCTAA 5606MI1_005Bacteroides Amino 18MATKEYFPGIGKIKFEGVNSYNPLAYRYYDAERIVLGKPMKEWLKFAMAWWHTLCAEGGD AcidQFGGGTKNFPWNGDPDPVQAAKNKVDAGFEFMTKMGIEYFCFHDVDLVSEAATIEEYEANLKEVVGYIKEKQAETGIKNLWGTANVFSHARYMNGAATNPDFDVVARAAVQIKNAIDATIALGGTNYVFWGGREGYMSLLNTDQKREKEHLAMMLRMARDYARAKGFTGTFLIEPKPMEPTKHQYDVDTETVIGFLRAHGLDKDFKVNIEVNHATLAGHTFEHELAVAVDNGMLGSIDANRGDYQNGWDTDQFPIDNYELTQAMMQIIRNGGFGNGGCNFDAKTRRNSTDLEDIFIAHIAGMDAMARALLSAAEVLEKSPYRKMLAERYAPFDAGQGKAFEEGAMSLTDLVEYAKEHGEPTQTSGKQELYEAIVNMYC 5606MI2_003 Bacteroides DNA 19ATGGCAACAAAGGAATATTTTCCCCATATAGGGAAGATCCAGTTCAAAGGCACGGAATCGTACGATCCGATGTCGTATCGTTACTATGACGCCGAGCGCGTAGTTCTGGGCAAGCCCATGAAGGAATGGCTGAAATTCGCCATGGCATGGTGGCACACATTGTGCGCCGAGGGCGGCGACCAGTTCGGCGGCGGAACGAAGAAGTTCCCCTGGAACGAGGGCGAGGACGCCATGACCATCGCCAAGCAGAAGGCTGACGCCGGCTTCGAGATCATGCAGAAGCTCGGCATCGAGTATTTCTGCTTCCACGACATCGACCTGATCGGCGACCTGGGCGACGACATCGAGGACTATGAGAACCGTATGCACGAAATCACCGCACACCTGAAGGAGAAGATGGCCGCCACGGGCATCAAGAACCTGTGGGGCACTGCCAACGTGTTCGGCCACGCACGCTATATGAACGGCGCCGCCACCAACCCCGACTTCGACGTTGTGGCACGCGCATGTGTGCAGATCAAGAACGCCATCGACGCCACCATCGCTCTAGGCGGTACAAACTATGTATTCTGGGGCGGCCGCGAGGGCTACATGAGCCTGCTGAACACCGACCAGAAGCGCGAGAAAGAGCACTTGGCTACCATGCTGACCATGGCACGCGACTATGCCCGCGCCAATGGCTTCACCGGAACGTTCCTGATCGAGCCCAAACCCATGGAGCCCAGCAAGCATCAGTATGATGTGGATACCGAGACCGTAATCGGCTTCCTGAAGGCCCACAACCTGGACAAGGACTTCAAGGTGAACATCGAGGTGAACCATGCCACTCTGGCCGGCCACACATTCGAGCATGAGCTGGCAGTAGCCGTGGACAACGGCATGCTGGGCAGCATCGACGCCAACCGCGGCGACTATCAGAACGGCTGGGACACCGACCAGTTCCCCATCGACAACTATGAGCTGACCCAGGCCATGATGCAGATAATCCGCAACGGTGGCCTCGGCAACGGCGGTACCAACTTCGACGCCAAGACACGTCGCAACTCCACCGACCTGGACGACATCTTCATCGCTCACATCGCCGGTATGGACGCTATGGCCCGCGCTCCGCTCAGCGCAGCCGACGTGCTTGAGAAGTCGCCTTACAAGAAGATGCTGGCCGACCGCTACGCTTCATTCGACAGCGGCGAGGGCAAGAAGTTCGAGGAAGGCAAGATGACTCTGGAGGATGTCGTGGCCTACGCCAAGAAGAATCCCGAACCCGCTCAGACCAGCGGCAAGCAGGAACTCTACGAGGCCATCATCAACATGTACGCCTGA 5606MI2_003Bacteroides Amino 20MATKEYFPHIGKIQFKGTESYDPMSYRYYDAERVVLGKPMKEWLKFAMAWWHTLCAEGGD AcidQFGGGTKKFPWNEGEDANTIAKQKADAGFEIMQKLGIEYFCFHDIDLIGDLGDDIEDYENRMHEITAHLKEKMAATGIKNLWGTANVFGHARYMNGAATNPDFDVVARACVQIKNAIDATIALGGTNYVFWGGREGYMSLLNTDQKREKEHLATMLTMARDYARANGFTGTFLIEPKPMEPSKHQYDVDTETVIGFLKAHNLDKDFKVNIEVNHATLAGHTFEHELAVAVDNGMLGSIDANRGDYQNGWDTDQFPIDNYELTQAMMQIIRNGGLGNGGTNFDAKTRRNSTDLDDIFIAHIAGMDAMARAPLSAADVLEKSPYKKMLADRYASFDSGEGKKFEEGKMTLEDVVAYAKKNPEPAQTSGKQELYEAIINMYA 5610MI3_003 Bacteroides DNA 21ATGGCAACAAAAGAATTTTTTCCCGAGATTGGTAAAATCAAGTTTGAGGGCCGCGAAAGCCGCAATCCCCTCGCATTCCGCTACTACGGCCCCGAGAAAGTCGTTCTTGGCAAGAAGATGAAAGACTGGTTCAAGTTTGCGATGGCTTGGTGGCACACACTGTGCGCCCAGGGCACCGACCAGTTTGGTGGCGACACCAAGCAGTTTCCGTGGAACACTGCCAGTGACCCCATGCAGGCCGCCAAGGATAAGGTGGATGCCGGATTTGAATTCATGACCAAGATGGGCATTGAGTACTTCTGCTTCCACGATGTGGATCTCGTCGCCGAGGCCGCCACTGTCGAGGAGTATGAGGCTAACCTCAAGACCATCGTCGCCTACATCAAAGAGAAACAAGCCGAGACCGGCATCAAGAACCTGTGGGGCACAGCCAACGTATTCGGACACAAACGCTACATGAACGGTGCCGCCACCAACCCCGACTTTGATGTCGTGGCACGCGCCATCGTGCAAATCAAGAACGCCATCGACGCCACCATCGAGTTGGGCGGCACGAGTTACGTCTTTTGGGGCGGCCGCGAGGGCCACATGAGCCTGCTCAACACCGACCAGAAGCGCGAGAAGGAGCACCTTGCACGCATGCTGACCATGGCACGCGACTATGCCCGCGCACGTGGTTTCAACGGCACCTTCCTCATCGAGCCCAAGCCCATGGAGCCGACCAAGCACCAATATGATGTGGACACCGAGACCGTCATCGGTTTCCTGCGTGCCCATGGTCTGGACAAGGACTTCAAGGTCAACATCGAGGTGAACCACGCTACACTGGCCGGACACACCTTCGAGCGCGAACTGGCAGTGGCCGTCGACAACGGTCTACTCGGCTCAATCGACGCCAACCGTGGTGACTATCAGAATGGTTGGGACACCGATCAGTTCCCCATCGACCACTATGAGTTGGTTCAGGGCATGTTGCAGATTATCCGCAATGGTGGTTTCACCGACGGTGGCACCAACTTCGATGCCAAGACCCGCCGCAACTCGACCGACCTCGAGGACATCTTCATCGCCCACATCGCCGCGATGGATGCCATGGCTCATGCGCTGGAGAGTGCTGCCTCCATCATCGAGGAGTCGCCCTACTGCCAGATGGTCAAGGATCGCTATGCCTCATTTGACTCCGGCATCGGCAAGGACTTTGAGGACGGCAAGTTGACACTGGAACAAGCCTACGAGTACGGTAAGCAAGTGGGCGAACCCAAGCAGACCAGTGGCAAGCAAGAACTGTACGAGTCAATCATCAATATGTATTCCATTTAA 5610MI3_003Bacteroides Amino 22MATKEFFPEIGKIKFEGRESRNPLAFRYYGPEKVVLGKKMKDWFKFAMAWWHTLCAQGTD AcidQFGGDTKQFPWNTASDPMQAAKDKVDAGFEFMTKMGIEYFCFHDVDLVAEAATVEEYEANLKTIVAYIKEKQAETGIKNLWGTANVFGHKRYMNGAATNPDFDVVARAIVQIKNAIDATIELGGTSYVFWGGREGHMSLLNTDQKREKEHLARMLTMARDYARARGFNGTFLIEPKPMEPTKHQYDVDTETVIGFLRAHGLDKDFKVNIEVNHATLAGHTFERELAVAVDNGLLGSIDANRGDYQNGWDTDQFPIDHYELVQGMLQIIRNGGFTDGGTNFDAKTRRNSTDLEDIFIAHIAAMDAMAHALESAASIIEESPYCQMVKDRYASFDSGIGKDFEDGKLTLEQAYEYGKQVGEPKQTSGKQELYESIINMYSI 5749MI2_004 Bacteroides DNA 23ATGGCAACAAAAGAGTATTTTCCTGGTATAGGAAAGATTAAATTTGAAGGTAAAGAGAGTAAGAATCCGATGGCATTCCGCTATTATGATGCCAATAAAGTAATCATGGGCAAGAAGATGAGCGAGTGGCTGAAGTTTGCCATGGCTTGGTGGCACACATTGTGCGCCGAAGGTGGTGACCAGTTTGGTGGTGGAACAAAGACTTTCCCGTGGAACGATTCGGACAACGCCGTAGAAGCAGCCAACCATAAAGTAGATGCCGGTTTTGAATTTATGCAGAAAATGGGCATCGAATACTATTGCTTCCATGATGTAGACCTCTGCACTGAAGCTGCTACCATTGAAGAATATGAAGCCAATCTGAAGGAAATAGTAGCCTATCCGAAACAGAAACAGGCTGAAACAGGTATCAAACTTCTGTGGGGTACGGCAAATGTATTTGGTCACAAACGCTATATGAATGGTGCTGCTACCAATCCGGATTTTGATGTAGTGGCTCGTGCTGCTGTACAGATTAAGAATGCGATAGACGCTACAATTGAACTCGGTGGTAGCAACTACGTGTTCTGGGGCGGCCGTGAAGGTTATATGAGCTTGCTCAATACAGACCAGAAACGTGAGAAAGAGCATTTGGCACAAATGTTGACCATGGCTCGTGACTATGCTCGTGCCAAAGGATTCAAGGGTACCTTCCTGGTTGAACCCAAACCGATGGAACCAACTAAACACCAGTATGATGTAGATACGGAAACTGTAATCGGCTTCCTCAAGGCTCATAATTTGGATAAGGATTTCAAGGTAAATATTGAAGTAAACCATGCTACATTGGCCGGTCATACTTTTGAACACGAATTGGCTGTTGCCGTAGACAACGATATGCTTGGCTCTATCGATGCCAACCGCGGTGACTATCAGAACGGTTGGGATACTGACCAGTTCCCCATTGACAACTTCGAGCTTATCCAAGCCATGATGCAGATTATTCGCGGTGGTGGCTTCAAAGATGGTGGTACAAACTTCGACGCTAAGACTCGTCGTAACTCTACCGACCTGGAAGATATTTTCATTGCACACATCGCTGGTATGGATGCTATGGCACGTGCTTTGGAAAGTGCAGCCAAGTTGCTTGAGGAATCTCCTTATAAGAAAATGTTGGCTGACCGCTATGCATCGTTCGATAGTGGCAAAGGTAAGGAGTTTGAAGAAGGCAAGCTGACATTGGAAGACGTTGTAGTTTATGCCAAGCAGAATGGCGAGCCTAAACAGACCAGCGGTAAGCAGGAATTGTATGAGGCAATTGTAAATATGTATGCCTGA 5749MI2_004Bacteroides Amino 24MATKEYFPGIGKIKFEGKESKNPMAFRYYDANKVIMGKKMSEWLKFAMAWWHTLCAEGGD AcidQFGGGTKTFPWNDSDNAVEAANHKVDAGFEFMQKMGIEYYCFHDVDLCTEAATIEEYEANLKEIVAYPKQKQAETGIKLLWGTANVFGHKRYMNGAATNPDFDVVARAAVQIKNAIDATIELGGSNYVFWGGREGYMSLLNTDQKREKEHLAQMLTMARDYARAKGFKGTFLVEPKPMEPTKHQYDVDTETVIGFLKAHNLDKDFKVNIEVNHATLAGHTFEHELAVAVDNDMLGSIDANRGDYQNGWDTDQFPIDNFELIQAMMQIIRGGGFKDGGTNFDAKTRRNSTDLEDIFIAHIAGMDAMARALESAAKLLEESPYKKMLADRYASFDSGKGKEFEEGKLTLEDVVVYAKQNGEPKQTSGKQELYEAIVNMYA 5750MI3_003 Bacteroides DNA 25ATGGCAACAAAAGAGTATTTTCCTGGAATAGGAAAGATTAAATTTGAAGGAAAAGAGAGTAAGAACCCGATGGCATTCCGTTGCTACGATGCAGAAAAAGTTATCATGGGTAAGAGAATGAAAGATTGGTTGAAGTTTGCAATGGCGTGGTGGCATACACTTTGTGCAGAAGGCGGTGACCAATTCGGTGGCGGTACAAAGAGTTTCCCCCGGAACGACTATACTGATAAAATTCAGGCTGCTAAAAACAAGATGGATGCCGGTTTTGAGTTTATGCAGAAGATGGGGATCGAATACTATTGTTTTCACGATGTAGACCTCTGCACGGAAGCTGATACCATTGAAGAATACGAAGCTAATTTGAAAGAAATCGTAGTTTACGCAAAGCAAAAGCAGGTAGAAACAGGTATCAAATTATTGTGGGGTACTGCCAATGTATTCGGTCATGAACGCTATATGAATGGTGCGGCTACCAACCCAGATTTTGATGTTGTAGCCCGTGCTGCTGTTCAGATTAAGAATGCAATTGATGCTACCATTGAACTAGGTGGCTTAAACTATGTGTTCTGGGGTGGACGCGAAGGTTATATGTCTTTGCTGAACACTGATCAGAAACGTGAGAAAGAACATCTTGCACAAATGCTGACCATTGCCCGTGACTATGCCCGTGCCCGTGGCTTCAAAGGTACATTCTTGGTTGAACCGAAACCGATGGAACCAACCAAACATCAATATGACGTAGATACAGAAACAGTTATCGGTTTTTTGAAAGCTCATGCTTTGGATAAAGACTTTAAAGTAAATATTGAAGTAAATCATGCAACATTAGCCGGTCATACATTTGAACACGAACTGGCAGTGGCTGTCGACAACGGTATGCTGGGTTCTATTGACGCTAATCGTGGTGATTGTCAAAACGGTTGGGATACAGACCAATTTCCCATTGATAACTATGAACTGACTCAAGCCATGATGCAGATTATTCGTAACGGTGGTTTGGGCAATGGTGGTACGAATTTTGACGCTAAAACTCGCCGTAATTCTACTGATCTTGGAGATATCTTCATTGCTCACATCGCAGGTATGGATGCTATGGCACGTGCATTGGAAAGTGCGGCCAAGTTGTTGGAAGAATCTCCCTATAAGAAGATGCTGGCAGAACGTTATGCATCCTTTGACAGCGGTAAGGGTAAAGAGTTTGAAGAGGGTAAGTTGACCTTGGAGGATCTTGTTGCTTATGCAAAAGTCAATGGCGAACCGAAACAAATCAGTGGTAAACAAGAATTGTATGAGGCAATTGTGAATATGTATTGCTAA 5750MI3_003Bacteroides Amino 26MATKEYFPGIGKIKFEGKESKNPMAFRCYDAEKVIMGKRMKDWLKFAMAWWHTLCAEGGD AcidQFGGGTKSFPRNDYTDKIQAAKNKMDAGFEFMQKMGIEYYCFHDVDLCTEADTIEEYEANLKEIVVYAKQKQVETGIKLLWGTANVEGHERYMNGAATNPDFDVVARAAVQIKNAIDATIELGGLNYVFWGGREGYMSLLNTDQKREKEHLAQMLTIARDYARARGFKGTFLVEPKPMEPTKHQYDVDTETVIGFLKAHALDKDFKVNIEVNHATLAGHTFEHELAVAVDNGMLGSIDANRGDCQNGWDTDQFPIDNYELTQAMMQIIRNGGLGNGGTNFDAKTRRNSTDLGDIFIAHIAGMDAMARALESAAKLLEESPYKKMLAERYASFDSGKGKEFEEGKLTLEDLVAYAKVNGEPKQISGKQELYEAIVNMYC 5750MI4_003 Bacteroides DNA 27ATGGCAACAAAAGAGTATTTTCCCGGAATAGGAAAGATTAAATTCGAAGGTAAAGAGAGCAAGAACCCGATGGCATTCCGTTATTACGATGCCGATAAAGTAATCATGGGTAAGAAAATGAGCGAATGGCTGAAGTTCGCCATGGCATGGTGGCACACTCTTTGCGCAGAAGGTGGTGACCAGTTCGGTGGCGGAACAAAGAAATTCCCCTGGAACGGTGAGGCTGACAAGGTTCAGGCTGCCAAGAACAAAATGGACGCCGGCTTTGAATTCATGCAGAAAATGGGTATCGAATACTACTGCTTCCACGATGTAGACCTCTGCGAAGAAGCCGAGACCATTGAAGAATACGAAGCCAACTTGAAGGAAATCGTAGCGTATGCCAAGCAGAAACAAGCAGAAACCGGCATCAAGCTGTTGTGGGGTACTGCCAACGTATTCGGCCATGCCCGCTACATGAATGGTGCAGCCACCAACCCCGATTTCGATGTTGTGGCACGTGCAGCCGTCCAAATCAAAAGCGCCATCGACGCTACTATCGAGCTGGGAGGTTCGAACTATGTGTTCTGGGGCGGTCGCGAAGGCTACATGTCATTGCTGAATACAGACCAGAAGCGTGAGAAAGAGCACCTCGCACAGATGTTGACCATCGCCCGCGACTATGCCCGTGCCCGTGGCTTCAAAGGTACCTTCCTGATTGAACCGAAACCGATGGAACCTACAAAACACCAGTATGATGTAGACACCGAAACCGTTATCGGCTTCTTGAAGGCCCACAATCTGGACAAAGATTTCAAGGTAAACATCGAAGTGAACCACGCTACTTTGGCGGGCCACACCTTCGAGCACGAACTCGCAGTAGCCGTAGACAACGGTATGCTCGGCTCCATCGATGCCAACCGTGGTGACTACCAGAACGGCTGGGATACAGACCAGTTCCCCATTGACAACTTCGAACTGACCCAGGCAATGATGCAAATCATCCGTAACGGCGGCTTTGGCAATGGCGGTACAAACTTCGATGCCAAGACCCGTCGTAACTCCACCGACCTGGAAGACATCTTGATTGCCCACATCGCCGGTATGGACGTGATGGCACGTGCACTGGAAAGTGCAGCCAAATTGCTTGAAGAGTCTCCTTACAAGAAGATGCTTGCCGACCGCTATGCTTCCTTCGACAGTGGTAAAGGCAAGGAATTCGAAGACGGCAAGCTGACACTGGAGGATTTGGCAGCTTACGCAAAAGCCAACGGTGAGCCGAAACAGACCAGCGGCAAGCAGGGATTGTATGAGGCAATCGTAAATATGTACTGCTGA 5750MI4_003Bacteroides Amino 28MATKEYFPGIGKIKFEGKESKNPMAFRYYDADKVIMGKKMSEWLKFAMAWWHTLCAEGGD AcidQFGGGTKKFPWNGEADKVQAAKNKMDAGFEFMQKMGIEYYCFHDVDLCEEAETIEEYEANLKEIVAYAKQKQAETGIKLLWGTANVFGHARYMNGAATNPDFDVVARAAVQIKSAIDATIELGGSNYVFWGGREGYMSLLNTDQKREKEHLAQMLTIARDYARARGFKGTFLIEPKPMEPTKHQYDVDTETVIGFLKAHNLDKDFKVNIEVNHATLAGHTFEHELAVAVDNGMLGSIDANRGDYQNGWDTDQFPIDNFELTQAMMQIIRNGGFGNGGTNFDAKTRRNSTDLEDIFIAHIAGMDVMARALESAAKLLEESPYKKMLADRYASFDSGKGKEFEDGKLTLEDLAAYAKANGEPKQTSGKQGLYEAIVNMYC 5751MI4_002 Bacteroides DNA 29ATGACAAAAGAGTATTTTCCAACCATTGGTAAAATTCAGTTTGAAGGTAAAGAGAGTAAGAATCCATTAGCATATCGTTATTACGATGCTAACAAAGTAATAATGGGTAAAAAGATGAGCGAATGGCTCAAGTTTGCAATGGCATGGTGGCACACTTTGTGTGCTGAGGGTAGCGACCAGTTTGGTCCTGGCACCAAGTCATTCCCATGGAACGCATCAACCGACCGTATGCAGGCTGCAAAAGATAAGGCTGACGCAGGCTTCGAAATCATGCAAAAACTGGGCATCGAATACTACTGTTTCCATGATGTTGACCTCATCGACCCAGCAGACGATATTCCAACATACGAAAAGAATCTCAAGGAAATCGTTGCATACCTCAAGCAAAAACAGGCCGAGACAGGTATCAAATTGCTATGGGGTACAGCTAACGTATTTGGCCACAAGCGTTATATGAACGGTGCATCTACCAATCCTGACTTTGACGTTGTTGCACGAGCTATCGTGCAAATCAAGAATGCTATCGATGCAACAATCGAACTGGGCGGCACGAACTACGTATTCTGGGGTGGTCGCGAAGGTTACATGTCACTGCTCAACACCGACCAAAAGCGCGAGAAAGAGCACATGGCTACCATGTTAGGAATGGCACGTGACTATGCACGTTCTAAAGGCTTTACTGGTACTCTCCTTATCGAGCCAAAGCCTATGGAACCAACTAAGCATCAATACGACGTCGATACAGAAACTGTTATTGGTTTCCTCAAAGCTCACGGATTAGACAAGGACTTCAAGGTAAATATCGAAGTGAACCACGCTACATTGGCTGGCCATACCTTCGAACATGAATTAGCATGTGCTGTTGATGCAGGTATGCTTGGTTCCATCGATGCTAACCGTGGTGATATGCAGAATGGCTGGGATACAGATCAGTTCCCTATCAACAATTACGAGCTCGTTCAGGCCATGATGCAGATTATCCGCAATGGTGGTTTCGGTAACGGTGGTACAAACTTCGACGCTAAGACACGTCGTAATTCAACCGATTTGGAAGACATCATCATTGCTCACGTTTCAGCTATGGATGCTATGGCACGTGCTCTTGAATGTGCTGCAGACATTCTTCAAAACTCACCTATTCCACAGATGGTGGCCAACCGTTATGCAAGTTTTGACAAGGGTATAGGTAAAGATTTCGAAGACGGCAAGCTCACCCTCGAGCAAGTATACGAATATGGTAAGACCGTCGGCGAACCAGCTATTACAAGCGGCAAACAGGAGCTCTACGAAGCTATCGTTAATATGTATTGCTGA 5751MI4_002Bacteroides Amino 30MTKEYFPTIGKIQFEGKESKNPLAYRYYDANKVIMGKKMSEWLKFAMAWWHTLCAEGSDQ AcidFGPGTKSFPWNASTDRMQAAKDKADAGFEIMQKLGIEYYCFHDVDLIDPADDIPTYEKNLKEIVAYLKQKQAETGIKLLWGTANVFGHKRYMNGASTNPDFDVVARAIVQIKNAIDATIELGGTNYVFWGGREGYMSLLNTDQKREKEHMATMLGMARDYARSKGFTGTLLIEPKPMEPTKHQYDVDTETVIGFLKAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANRGDMQNGWDTDQFPINNYELVQAMMQIIRNGGFGNGGTNFDAKTRRNSTDLEDIIIAHVSAMDAMARALECAADILQNSPIPQMVANRYASFDKGIGKDFEDGKLTLEQVYEYGKTVGEPAITSGKQELYEAIVNMYC 5751MI5_003 Bacteroides DNA 31ATGGCTAACAAAGAATTTTTCCCCGGTATTGGTAAAATCAAATTCGAAGGTAAAGAGAGCAAGAACCCCATGGCATATCGTTACTACGATGCTGAGAAGGTAGTCCTTGGCAAGAATATGAAAGACTGGTTCAAGTTTGCGATGGCTTGGTGGCACACATTGTGCGCCGAGGGTAGCGACCAGTTTGGTCCCGGCACTAAGTCTTTCCCCTGGAACACCGCAGAGTGCCCCATGCAGGCAGCTAAGGACAAGGTTGACGCTGGCTTCGAGTTCATGACCAAGATGGGTATTGAATACTTCTGCTTCCACGATGTAGACCTCGTTGCCGAGGCCGACACTGTTGAGGAGTACGAGGCTCGCATGAAGGAAATCGTTGCTTACATCAAGGAGAAGGTGGCCGAGACTGGCATCAAGAACCTGTGGGGTACAGCTAACGTATTTGGCAACAAGCGCTACATGAACGGTGCTGCTACTAACCCCGACTTTGACGTTGTGGCTCGCGCTATCGTTCAAATCAAGAACGCTATCGACGCTACTATCGAGCTCGGTGGTACGTCATACGTATTCTGGGGCGGCCGCGAGGGTTACATGAGCCTCTTGAACACCGACCAGAAGCGTGAGAAAGAGCACCTGGCTACTATGCTCACTATGGCACGCGACTACGCTCGCGCTAAGGGTTTCAAGGGTACATTCCTCATCGAGCCCAAGCCCATGGAGCCCACAAAGCACCAGTACGATGTTGACACTGAGACTGTAATCGGCTTCCTTAAGGCACACAACCTTGACAAGGACTTCAAGGTTAACATTGAGGTTAACCACGCAACTCTCGCTGGTCACACATTTGAGCACGAGCTCGCTTGTGCTGTTGACGCTGGCATGCTTGGCAGCATCGACGCTAACCGCGGTGACTACCAGAACGGCTGGGATACTGACCAATTCCCCATCGACAACTTCGACCTCACTCAAGCTATGCTCGAGATCATCCGCAACGATGGTTTCAAGGATGGTGGTACAAACTTCGACGCTAAGACTCGCCGCAACAGCACCGACCTCGAGGATATCTTCATCGCACACATCGCTGCTATGGACGCTATGGCACGTGCTCTCGAGAGCGCTGCTGCAGTACTCGAGGAGTCAGCTCTGCCCCAAATGAAGAAGGACCGCTATGCATCGTTCGACGCTGGCATGGGTAAGGACTTCGAGGACGGCAAGCTCACCCTGGAGCAAGTTTACGAGTATGGTAAGAAGGTGGGCGAGCCCAAGCAGACTAGCGGCAAGCAAGAGCTGTATGAGGCTATCCTCAACATGTACGTATAA 5751MI5_003Bacteroides Amino 32MANKEFFPGIGKIKFEGKESKNPMAYRYYDAEKVVLGKNMKDWFKFAMAWWHTLCAEGSD AcidQFGPGTKSFPWNTAECPMQAAKDKVDAGFEFMTKMGIEYFCFHDVDLVAEADTVEEYEARMKEIVAYIKEKVAETGIKNLWGTANVFGNKRYMNGAATNPDFDVVARAIVQIKNAIDATIELGGTSYVFWGGREGYMSLLNTDQKREKEHLATMLTMARDYARAKGFKGTFLIEPKPMEPTKHQYDVDTETVIGFLKAHNLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANRGDYQNGWDTDQFPIDNFDLTQAMLEIIRNDGFKDGGTNFDAKTRRNSTDLEDIFIAHIAAMDAMARALESAAAVLEESALPQMKKDRYASFDAGMGKDFEDGKLTLEQVYEYGKKVGEPKQTSGKQELYEAILNMYV 5751MI6_004 Bacteroides DNA 33ATGGCTAACAAAGAATTTTTCCCAGGTATTGGTAAAATCAAATTCGAAGGCAAAGAAAGCAAGAACCCCATGGCATATCGTCACTACGATGCCGAGAAGGTAGTCCTTGGTAAGAAGATGAAGGACTGGTTCAAGTTTGCGATGGCTTGGTGGCACACTCTGTGCGCCGAGGGTAGCGACCAGTTCGGCCCCGTGACCAAGTCTTTCCCCTGGAACCAGGCCGAGTGCCCCATGCAGGCTGCTAAGGACAAGGTTGACGCCGGCTTCGAGTTCATGACCAAGATGGGTATCGAATACTTCTGTTTCCACGATGTAGACCTCGTTGCCGAGGCCGACACCGTTGAGGAGTACGAAGCTCGCATGAAGGAAATCGTGGCTTACATCAAGGAGAAGATGGCCGAGACCGGCATCAAGAACCTGTGGGGTACAGCCAACGTATTCGGCAACAAGCGCTACATGAACGGTGCTGCCACCAACCCCGACTTTGACGTTGTGGCTCGCGCAATCGTTCAGATCAAGAACGCCATCGACGCTACTATCGAGCTCGGCGGTACCTCTTACGTGTTCTGGGGCGGCCGCGAGGGTTACATGACTCTCTTGAACACCGACCAGAAGCGCGAGAAGGAGCACCTGGCTACCATGCTCACCATGGCTCGCGACTATGCTCGCGCTAAGGGCTTCAAGGGTACATTCCTTATCGAGCCCAAGCCCATGGAGCCCACCAAGCACCAGTATGACGTGGATACCGAGACCGTTATCGGCTTCCTCAAGGCTCACGGCCTGGACAAGGACTTCAAGGTGAACATCGAGGTTAACCATGCAACTCTCGCCGGCCACACATTCGAGCACGAACTCGCTTGCGCTGTTGACGCTGGCATGCTGGGCAGCATCGACGCTAACCGCGGCGACTACCAGAACGGCTGGGATACCGACCAGTTCCCCATCGACAACTTCGACCTCACTCAGGCTATGCTCGAGATCATCCGCAACGGTGGTTTCAAGGACGGTGGTACAAACTTCGACGCTAAGACCCGTCGCAACAGCACCGATCTTGAGGACATCTTCATCGCTCACATCGCTGCTATGGACGCAATGGCACGCGCGCTCGAGAGCGCTGCCGCTGTGCTCGAGCAGAGCCCCCTTCCCCAGATGAAGAAAGACCGCTACGCATCGTTCGATGCCGGCATGGGCAAGGACTTCGAGGACGGCAAGCTCACTCTGGAGCAGGTTTACGAGTATGGTAAGAAGGTAGGCGAGCCCAAGCAGACCAGCGGCAAGCAGGAACTGTACGAGGCTATCCTCAACATGTATGTATAA 5751MI6_004Bacteroides Amino 34MANKEFFPGIGKIKFEGKESKNPMAYRHYDAEKVVLGKKMKDWFKFAMAWWHTLCAEGSD AcidQFGPVTKSFPWNQAECPMQAAKDKVDAGFEFMTKMGIEYFCFHDVDLVAEADTVEEYEARMKEIVAYIKEKMAETGIKNLWGTANVFGNKRYMNGAATNPDFDVVARAIVQIKNAIDATIELGGTSYVFWGGREGYMTLLNTDQKREKEHLATMLTMARDYARAKGFKGTFLIEPKPMEPTKHQYDVDTETVIGFLKAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANRGDYQNGWDTDQFPIDNFDLTQAMLEIIRNGGFKDGGTNFDAKTRRNSTDLEDIFIAHIAAMDAMARALESAAAVLEQSPLPQMKKDRYASFDAGMGKDFEDGKLTLEQVYEYGKKVGEPKQTSGKQELYEAILNMYV 5586MI22_003 Clostridiales DNA 35ATGAAAGAATATTTTCCTATGACAAAAAAAGTTGAATATGAGGGCGCAGCATCTAAAAATCCATTTGCGTTTAAATACTATGATGCCGAAAGAATTATAGCAGGCAAGCCTATGAAAGAACATCTTAAATTTGCTATGAGTTGGTGGCATACACTTTGTGCGGGCGGTGCAGACCCATTTGGCACAACAACTATGGACAGAACATACGGCGGACTTACCGACCCAATGGAAATTGCAAAGGCAAAAGTAGATGCAGGCTTTGAGTTTATGCAAAAACTCGGTATAGAGTATTTTTGTTTTCACGATGCGGATATTGCACCGGAAGGAAGCAGTTTTGTTGAAACAAAGAAAAACTTTTGGGAAATAGTAGATTATATACAGCAAAAGATGAATGAAACAGGCATAAAGTTGCTTTGGGGTACTGCAAACTGCTTTAATGCTCCACGTTATATGCACGGTGCAGGAACATCATGCAATGCGCACAGTTTTGCATATGCAGCCGCACAGATAAAAAATGCAATTGAAGCTACCGTTAAACTGGGTGGAAAAGGCTATGTTTTCTGGGGCGGAAGAGAGGGTTATGAAACACTTCTCAATACGGATATGGCACTTGAACTTGACAATATGGCAAGACTTATGCATATGGCAGTTGATTATGGCAGAAGCATTGGTTTTGACGGTGATTTTTATATCGAACCAAAGCCAAAGGAACCAACAAAACATCAATATGACTTTGACTCGGCAACTGTTTTGGGATTTTTGAGAAAGTACGGTTTAGATAAGGATTTTAAACTTAATATAGAGGCAAATCATGCGACACTTGCAGGTCATACATTTGAACATGAATTGACTGTAGCGCGTATAAACGGTGCATTTGGCAGCATAGATGCAAATAGCGGCGATCCCAATCTTGGCTGGGATACCGACCAATTCCCAACAGATGTTTATTCGGCAACCCTTTGTATGCTTGAAGTGATAAGAGCAGGCGGCTTTACAAACGGAGGTCTTAATTTTGATGCAAAGGTCAGAAGAGGCTCATTTACGTTTGATGACATTGTTTATGCATATATCAGCGGTATGGACACTTTTGCGCTGGGTTTTATAAAGGCATATGAAATAATTGAGGACGGCAGAATAGATGAATTTGTAAAAGAAAGATACGCAAGCTATAATACAGGCATAGGCAAAGATATTATAGATGGAAAGGCAAGCCTTGAAAGTTTGGAAGAATATATTCTTTCAAATGATAATGTTGTAATGCAAAGCGGCAGACAGGAATATCTTGAAACAGTTTTGAATAATATTTTGTTTAAAGCATAA5586MI22_003 Clostridiales Amino 36MKEYFPMTKKVEYEGAASKNPFAFKYYDAERIIAGKPMKEHLKFAMSWWHTLCAGGADPF AcidGTTTMDRTYGGLTDPMEIAKAKVDAGFEFMQKLGIEYFCFHDADIAPEGSSFVETKKNFWEIVDYIQQKMNETGIKLLWGTANCFNAPRYMHGAGTSCNAHSFAYAAAQIKNAIEATVKLGGKGYVFWGGREGYETLLNTDMALELDNMARLMHMAVDYGRSIGEDGDFYIEPKPKEPTKHQYDFDSATVLGELRKYGLDKDFKLNIEANHATLAGHTFEHELTVARINGAFGSIDANSGDPNLGWDTDQFPTDVYSATLCMLEVIRAGGFTNGGLNFDAKVRRGSFTFDDIVYAYISGMDTFALGFIKAYEIIEDGRIDEFVKERYASYNTGIGKDIIDGKASLESLEEYILSNDNVVMQSGRQEYLETVLNNILFKA 1753MI4_001 Firmicutes DNA 37ATGAAAGAAATTTTCCCAAATATTCCTGAGATTAAATTCGAAGGAAAAGACAGCAAAAATCCTTTTGCTTTCCATTACTACAACCCAGACCAAATCATCTTAGGCAAACCAATGAAAGAACACCTCCCATTCGCTATGGCTTGGTGGCACAATCTTGGTGCAACAGGTGTTGATATGTTTGGCGCTGGCCCAGCTGATAAGAGTTTCGGTGCTAAAGTTGGCACAATGGAACACGCTAAGGCCAAAGTCGATGCCGGTTTCGAATTCATGAAGAAACTCGGTATCAGATATTTCTGCTTCCATGATGTTGACTTAGTTCCAGAATGTGCAGATATCAAAGATACAAACAAAGAATTAGATGAAATCAGTGACTACATCTTAGAAAAGATGAAAGGCACAGATATTAAGTGTTTATGGGGCACCGCCAATATGTTCTCTAACCCACGCTTCTGCAATGGTGCGGGTTCCACAAACAGTGCGGATGTCTTCGCTTTCGCCGCTGCTCAAGTTAAGAAAGCCTTAGATATCACCGTTAAATTAGGTGGTAGGGGTTACGTCTTCTGGGGTGGTCGTGAAGGTTACGAAACATTACTCAATACAGACGTTAAATTCGAACAAGAAAACATTGCTCGTTTAATGAAGATGGCTGTTGAATATGGCCGTTCCATCGGTTTCAAAGGCGATTTCTATATCGAACCAAAACCAAAAGAACCAATGAAACACCAATATGACTTCGACGCCGCTACAGCTATTGGCTTCTTAAGAGCCCACGGCTTAGACAAAGACTTCAAGTTGAACATCGAAGCTAACCACGCTACATTAGCGGGTCATACATTCCAACACGATTTAAGAATCTCCGCCATTAATGGTATGTTAGGTTCTATCGATGCTAACCAAGGCGATATGCTCTTAGGTTGGGATACAGACGAATTCCCATTTGATGTCTACAGTGCGACACAATGTATGTACGAAGTCTTAAAGAATGGTGGTCTTACAGGTGGTTTCAACTTTGACTCCAAAACACGTCGTCCATCCTACACAATGGAAGATATGTTCTTAGCCTATATCTTAGGTATGGATACATTCGCTTTAGGTTTAATCAAAGCTGCTCAAATCATCGAAGATGGCCGTATTGATCAATTCATCGAAAAGAAATATTCTTCCTTCCGTGAAACAGAAATCGGTCAAAAGATCTTAAACAACAAGACAAGCTTAAAAGAATTATCCGATTACGCTTGCAAGATGGGTGCTCCAGAACTTCCAGGTAGTGGTCGTCAAGAAATGCTCGAAGCCATCGTTAACGATGTCTTATTCGGCAAG TAA1753MI4_001 Firmicutes Amino 38MKEIFPNIPEIKFEGKDSKNPFAFHYYNPDQIILGKPMKEHLPFAMAWWHNLGATGVDMF AcidGAGPADKSFGAKVGTMEHAKAKVDAGFEFMKKLGIRYFCFHDVDLVPECADIKDTNKELDETSDYILEKMKGTDIKCLWGTANMESNPRECNGAGSTNSADVFAFAAAQVKKALDITVKLGGRGYVFWGGREGYETLLNTDVKFEQENIARLMKMAVEYGRSIGFKGDFYIEPKPKEPMKHQYDFDAATAIGFLRAHGLDKDFKLNIEANHATLAGHTFQHDLRISAINGMLGSIDANQGDMLLGWDTDEFPFDVYSATQCMYEVLKNGGLTGGFNFDSKTRRPSYTMEDMFLAYILGMDTFALGLIKAAQIIEDGRIDQFIEKKYSSFRETEIGQKILNNKTSLKELSDYACKMGAPELPGSGRQEMLEAIVNDVLFGK 1753MI6_001 Firmicutes DNA 39ATGAAAGAAATTTTCCCAAATATTCCTGAGATTAAATTCGAAGGAAAAGACAGCAAAAATCCTTTTGCTTTCCATTACTACAACCCAGACCAAATCATCTTAGGTAAACCAATGAAAGAACACCTCCCATTCGCTATGGCTTGGTGGCACAATCTTGGTGCAACAGGTGTTGATATGTTTGGCGCTGGCCCAGCTGATAAGAGTTTCGGTGCTAAAGTTGGCACAATGGAACACGCTAAGGCCAAAGTCGATGCCGGTTTCGAATTCATGAAGAAACTTGGTATCAGATATTTCTGCTTCCATGATGTTGACTTAGTTCCAGAATGTGCAGATATCAAAGATACAAACAAAGAATTAGATGAAATCAGTGACTACATCTTAGAAAAGATGAAAGGCACAGATATCAAGTGTTTATGGGGCACCGCCAATATGTTCTCTAACCCACGTTTCTGCAATGGTGCGGGTTCCACAAACAGTGCGGATGTCTTCGCTTTCGCCGCTGCTCAAGTTAAGAAAGCCTTAGATATCACCGTTAAATTAGGTGGTAGGGGTTACGTCTTCTGGGGTGGTCGTGAAGGTTACGAAACATTACTCAATACAGACGTTAAATTCGAACAAGAAAACATTGCTCGTTTAATGAAGATGGCTGTTGAATATGGCCGTTCCATCGGTTTCAAAGGCGATTTCTATATCGAACCAAAACCAAAAGAACCAATGAAACACCAATATGACTTCGACGCCGCTACAGCTATTGGCTTCTTAAGAGCCCACGGCTTAGACAAAGACTTCAAGTTGAACATCGAAGCTAACCACGCTACATTAGCGGGTCATACATTCCAACACGATTTAAGAATCTCCGCCATTAATGGTATGTTAGGTTCTATCGATGCTAACCAAGGCGATATGCTCTTAGGTTGGGATACAGACGAATTCCCATTTGATGTCTACAGTGCGACACAATGTATGTACGAAGTCTTAAAGAATGGTGGTCTTACAGGTGGTTTCAACTTTGACTCCAAAACACGTCGTCCATCCTACACAATGGAAGATATGTTCTTAGCCTATATCTTAGGTATGGATACATTCGCTTTAGGTTTAATCAAAGCTGCTCAAATCATCGAAGATGGCCGTATTGATCAATTCATCGAAAAGAAATATTCTTCCTTCCGTGAAACAGAAATCGGTCAAAAGATCTTAAACAACAAGACAAGCTTAAAAGAATTATCCGATTACGCTTGCAAGATGGGTGCTCCAGAACTTCCAGGTAGTGGTCGTCAAGAAATGCTCGAAGCCATCGTTAACGATGTCTTATTCGGCAAG TAA1753MI6_001 Firmicutes Amino 40MKEIFPNIPEIKFEGKDSKNPFAFHYYNPDQIILGKPMKEHLPFAMAWWHNLGATGVDMF AcidGAGPADKSFGAKVGTMEHAKAKVDAGFEFMKKLGIRYFCFHDVDLVPECADIKDTNKELDEISDYILEKMKGTDIKCLWGTANMFSNPRFCNGAGSTNSADVFAFAAAQVKKALDITVKLGGRGYVFWGGREGYETLLNTDVKFEQENIARLMKMAVEYGRSIGFKGDFYIEPKPKEPMKHQYDFDAATAIGFLRAHGLDKDFKLNIEANHATLAGHTFQHDLRISAINGMLGSIDANQGDMLLGWDTDEFPFDVYSATQCMYEVLKNGGLTGGFNFDSKTRRPSYTMEDMFLAYILGMDTFALGLIKAAQIIEDGRIDQFIEKKYSSFRETEIGQKILNNKTSLKELSDYACKMGAPELPGSGRQEMLEAIVNDVLFGK 1753MI35_004 Firmicutes DNA 41ATGGAATATTTCCCTTTCGTCAAATCGGTCCAATACAAGGGACCAACCTCAACTGAACCATTCGCTTTCAAGTACTACGATGCCAACCGTGTCGTTCTTGGAAAACCAATGAAAGAATGGATGCCATTCGCTATGGCTTGGTGGCACAACCTCGGCGCTGCCGGTACCGACATGTTCGGCGGCAACACCATGGACAAGTCCTGGGGAGTCGATAAAGAAAAAGACCCAATGGGCTATGCCAAAGCCAAAGTTGATGCCGGCTTCGAATTCATGCAGAAGATGGGCATCGAATACTACTGCTTCCACGATGTCGACCTCGTCCCAGAGTGCGACGACATCACCGTTATGTACCAGAGACTCGATGAGATCGGTGATTACCTTCTCAAGAAACAGAAGGAAACCGGTATCAAGCTTCTTTGGTCAACCGCCAATGCCTTCGGACACCGCCGTTTCATGAACGGTGCTGGTTCCAGCAACTCCGCCGAAGTCTATTGCTTCGCCGCCGCCCAGATCAAGAAAGCTCTTGAGCTCTGCGTCAAACTCGGTGGCAAAGGCTATGTCTTCTGGGGTGGACGTGAAGGCTACGAAACCCTTCTCAACACCGACATGAAGTTCGAACAAGAGAACATCGCCAACCTTATGAGATGCGCCCGTGACTACGGCCGCAAGATCGGTTTCAAAGGCGACTTCTACATCGAACCAAAACCAAAAGAGCCAACAAAGCATCAGTATGACTTCGACGCCGCTACCGCCATCGGATTCCTCCGTCAGTACGGTCTCGACAAAGACTTCAAGATGAACATCGAAGCCAACCACGCTACCTTAGCTGGCCACACCTTCGAACACGAACTCCGCGTCTCCGCCATGAACGGCATGCTCGGTTCCATCGACGCCAACGAAGGCGATATGCTCCTCGGATGGGATGTCGACCGTTTCCCAGCCAACGTCTATAGCGCCACCTTCGCCATGCTCGAAGTCATCAAAGCCGGTGGACTTACCGGTGGCTTCAACTTCGACGCCAAGACCCGCCGCGCTTCCAACACCTATGAAGATATGTTCAAGGCTTTCGTCCTTGGTATGGATACCTTCGCTTTAGGTCTTCTCAATGCCGAAGCCATCATCAAAGACGGCCGCATCGACAAGTTCGTCGAGGATAGATATGCCAGCTTCAAGACCGGCATCGGTGCTAAGGTCCGCGATCACTCCGCTACCCTTGAGGATTTAGCTGCCCACGCCCTTGAGACCAAGGTTTGCCCAGATCCAGGCAGCGGCGACGAGGAAGAACTCCAGGAAATCCTCAACCAGTTAATGTTCGGTAAG AAATAA1753MI35_004 Firmicutes Amino 42MEYFPEVESVQYKGPTSTEPFAFKYYDANRVVLGKPMKEWMPFAMAWWHNLGAAGTDMFG AcidGNTMDKSWGVDKEKDPMGYAKAKVDAGFEFMQKMGIEYYCFHDVDLVPECDDITVMYQRLDEIGDYLLKKQKETGIKLLWSTANAFGHRRFMNGAGSSNSAEVYCFAAAQIKKALELCVKLGGKGYVFWGGREGYETLLNTDMKFEQENIANLMRCARDYGRKIGFKGDFYIEPKPKEPTKHQYDFDAATAIGFLRQYGLDKDFKMNIEANHATLAGHTFEHELRVSAMNGMLGSIDANEGDMLLGWDVDRFPANVYSATFAMLEVIKAGGLTGGFNFDAKTRRASNTYEDMFKAFVLGMDTFALGLLNAEAIIKDGRIDKFVEDRYASFKTGIGAKVRDHSATLEDLAAHALETKVCPDPGSGDEEELQEILNQLMFGKK 1754MI9_004 Firmicutes DNA 43ATGAGCGAATTTTTTAAGAATATTCCAGAGATTAAATTCGAAGGAAAAGATAGTAAAAATCCATGGGCATTCAAGTATTACAATCCTGAATTGACCATTATGGGTAAAAAAATGTCTGAACATCTTCCTTTTGCAATGGCCTGGTGGCATAACCTTGGCGCAAATGGAGTTGATATGTTCGGTTCGGGAACCGCCGATAAATCTTTCGGTCAGGCTCCGGGAACTATGGAGCACGCAAAGGCTAAGGTAGATGCAGGTATCGAGTTTATGAAGAAACTCGGAATCAAGTACTACTGCTGGCATGATGTAGACCTTGTTCCTGAAGATCCAAACGATATCAACGTAACAAACAAGCGCCTTGATGAGATTTCAGATTATATCCTTGAAAAAACAAAGGGAACTGACATCAAGTGTCTCTGGGGAACTGCTAACATGTTCAGTAATCCCCGCTTTATGAACGGGGCAGGCTCAACAAACTCTGCTGACGTTTACTGCTTTGCAGCTGCCCAGGTTAAAAAGGCTCTTGAGATTACCGTAAAGCTTGGTGGCCGCGGTTATGTATTCTGGGGTGGACGCGAAGGTTATGAAACTCTTCTTAATACAGATGTAAAGCTTGAACAGGAAAATATTGCAAACCTTATGCACATGGCAGTTGATTATGGCCGTTCAATCGGTTTCAAGGGAGACTTCTACATCGAGCCTAAGCCAAAGGAGCCGATGAGTCATCAGTATGATTTTGATGCCGCAACTGCAATCGGCTTCCTCCGCCAGTATGGCCTCGACAAAGACTTTAAGATGAACATTGAGGCTAACCACGCTTCTCTTGCAAATCATACCTTCCAGCATGAGCTTTATATCAGCCGCATTAACGGAATGCTTGGTTCTGTAGATGCTAACCAGGGAAATCCAATTCTCGGCTGGGATACAGATAACTTCCCTTGGAATGTCTACGACGCAACTCTTGCAATGTACGAAGTACTCAAGGCTGGTGGACTTACAGGTGGCTTCAACTTTGACTCAAAGAACCGCCGCCCATCAAATACATTTGAAGATATGTTCCACGCTTACATCATGGGAATGGACACTTTTGCTCTTGGTCTTATTAAGGCTGCAGAAATTATTGAAGACGGAAGAATCGATGGCTTCATTAAAGAAAAGTATTCAAGCTACGAAAGTGGAATTGGTAAGAAGATCCGCGACAAGCAGACAACTTTGGAAGAGCTTGCTGCCCGTGCCGCAGAAATGAAAAAGCCATCTGATCCAGGTTCAGGCCGCGAGGAATATCTGGAAGGAGTTGTTAACAATATCCTCTTTCGCGGA TAA1754MI9_004 Firmicutes Amino 44MSEFFKNIPEIKFEGKDSKNPWAFKYYNPELTIMGKKMSEHLPFAMAWWHNLGANGVDMF AcidGSGTADKSFGQAPGTMEHAKAKVDAGIEFMKKLGIKYYCWHDVDLVPEDPNDINVTNKRLDEISDYILEKTKGTDIKCLWGTANMFSNPRFMNGAGSTNSADVYCFAAAQVKKALEITVKLGGRGYVFWGGREGYETLLNTDVKLEQENIANLMHMAVDYGRSIGFKGDFYIEPKPKEPMSHQYDFDAATAIGFLRQYGLDKDFKMNIEANHASLANHTFQHELYISRINGMLGSVDANQGNPILGWDTDNFPWNVYDATLAMYEVLKAGGLTGGFNFDSKNRRPSNTFEDMFHAYIMGMDTFALGLIKAAEIIEDGRIDGFIKEKYSSYESGIGKKIRDKQTTLEELAARAAEMKKPSDPGSGREEYLEGVVNNILFRG 1754MI22_004 Firmicutes DNA 45ATGAGCGAGTTTTTTAAGAATATTCCTCAAATAAAATACGAAGGAAAAGATAGCAAAAATCCCTGGGCATTCAAGTATTACAATCCTGAATTGACAATCATGGGTAAAAAGATGAGCGAACATCTTCCATTCGCAATGGCATGGTGGCATAACCTTGGCGCAAACGGCGTTGATATGTTTGGTCAGGGAACAGCAGACAAGTCTTTCGGACAGATTCCTGGAACTATGGAGCATGCAAAGGCTAAGGTTGATGCTGGTATAGAGTTTATGAAGAAGCTCGGAATCAAATATTACTGCTGGCACGATGTTGACCTTGTTCCTGAGGATCCAAACGATATCAACGTAACTAACAAACGTCTGGACGAAATTTCAGATTACATCCTTGAAAAGACAAAAGGAACAGACATTAAGTGTCTCTGGGGAACTGCAAACATGTTCGGTAACCCTCGCTTTATGAACGGTGCAGGCTCTACAAACTCTGCTGACGTTTACTGTTTTGCTGCCGCTCAGGTAAAAAAGGCTCTTGAGATTACTGTAAAGCTTGGTGGCCGAGGTTATGTTTTCTGGGGTGGCCGCGAAGGTTACGAAACTCTTCTCAATACAGACGTAAAACTTGAACAGGAAAATATCGCAAACCTCATGCATATGGCTGTTGATTATGGCCGCTCAATCGGTTTCAAGGGAGACTTCTACATCGAGCCTAAGCCAAAGGAGCCAATGAGCCATCAGTATGATTTTGATGCTGCAACAGCAATCGGCTTCCTCCGCCAGTATGGCCTCGACAAAGATTTTAAGATGAACATCGAAGCTAACCATGCCTCACTTGCAAATCACACCTTCCAGCACGAGCTTTGTATCAGCCGCATAAACGGAATGCTTGGTTCTGTAGATGCAAATCAGGGAAATCCAATTCTTGGCTGGGATACAGATAACTTCCCATGGAATGTTTACGATGCAACTCTGGCAATGTACGAAGTTCTCAAGGCTGGCGGTCTAACAGGTGGCTTCAACTTTGACTCAAAGAACCGTCGCCCATCAAATACTTTTGAAGATATGTTCCACGCTTATATCATGGGTATGGATACTTTTGCCCTTGGCCTTATTAAGGCTGCAGAAATTATTGAAGACGGCAGAATTGACGGCTTCATCAAAGAAAAGTATTCAAGCTTTGAAAGTGGAATTGGTAAGAAGATTCGTGACAAGCAGACAAGTTTGGAAGAGCTTGCAGCTCGTGCCGCTGAAATGAAAAAGCCATCTGATCCAGGTTCAGGCCGCGAGGAATACCTCGAAGGAGTTGTTAACAACATCCTCTTTCGCGGA TAA1754MI22_004 Firmicutes Amino 46MSEFFKNIPQIKYEGKDSKNPWAFKYYNPELTIMGKKMSEHLPFAMAWWHNLGANGVDMF AcidGQGTADKSFGQIPGTMEHAKAKVDAGIEFMKKLGIKYYCWHDVDLVPEDPNDINVTNKRLDEISDYILEKTKGTDIKCLWGTANMEGNPRFMNGAGSTNSADVYCFAAAQVKKALEITVKLGGRGYVFWGGREGYETLLNTDVKLEQENIANLMHMAVDYGRSIGFKGDFYIEPKPKEPMSHQYDFDAATAIGFLRQYGLDKDFKMNIEANHASLANHTFQHELCISRINGMLGSVDANQGNPILGWDTDNFPWNVYDATLAMYEVLKAGGLTGGENFDSKNRRPSNTFEDMFHAYIMGMDTFALGLIKAAEIIEDGRIDGFIKEKYSSFESGIGKKIRDKQTSLEELAARAAEMKKPSDPGSGREEYLEGVVNNILFRG 727MI1_002 Firmicutes DNA 47ATGATATTTGAAAATATTCCCGCAATTCCTTATGAGGGTCCGAAGAGCACAAATCCGCTGGCGTTTAAATTCTATGATCCGGACAAGATCGTTATGGGAAAGCCCATGAAGGAGCATCTGCCCTTTGCAATGGCCTGGTGGCACAACCTTGGCGCGGCCGGAACCGATATGTTCGGGCGCGATACCGCCGACAAATCCTTCGGTGCGGTAAAAGGCACAATGGAGCATGCCAAAGCGAAAGTCGATGCCGGCTTTGAGTTCATGCAGAAGCTGGGGATCCGCTATTTCTGCTTCCATGATGTGGATCTTGTTCCGGAGGCGGATGATATAAAGGAGACCAACCGCCGTCTGGACGAGATCAGCGATTACATCCTTGAAAAGATGAAGGGCACCGATATCAAGTGCCTTTGGGGCACGGCCAATATGTTCTCAAATCCGCGCTTTATGAACGGCGCAGGCTCCTCCAATTCTGCCGATGTATTCGCTTTTGCGGCAGCACAGGCCAAGAAGGCCTTGGATCTGACCGTCAAACTCGGCGGGCGCGGCTATGTCTTCTGGGGCGGACGTGAGGGCTATGAGACACTTCTCAATACCGACATGAAGTTCGAGCAGGAGAATATCGCGAAGCTCATGCATATGGCTGTCGATTACGGCCGCAGCATAGGCTTTACCGGTGATTTCTATATCGAGCCCAAACCGAAAGAGCCGATGAAACACCAGTATGATTTCGATGCAGCCACTGCGATAGGCTTCCTCCGCCAGTACGGACTCGATAAGGACTTCAAGCTCAACATCGAGGCAAACCACGCCACACTGGCAGGTCACACTTTCCAGCACGATCTGCGTGTTTCCGCAATAAACGGAATGCTGGGCAGCATTGACGCCAACCAGGGCGATATGCTCCTCGGCTGGGATACCGACGAGTTCCCGTTCAATGTATATGATGCGACCATGTGCATGTATGAGGTGCTCAAGTCAGACGGGCTCACCGGCGGCTTTAACTTCGACTCCAAATCACGCCGCCCGAGCTATACGGTCGAGGATATGTTTACAAGCTATATCCTCGGCATGGACACTTTTGCCCTCGGCCTTCTGAAAGCGGCCGAGCTTATCGAAGACGGAAGGCTTGACGCCTTCGTCAAAGAACGCTATTCAAGCTATGAGAGCGGCATCGGCGCAAAGATCCGCAGCGGAGAAACCGATTTGAAGGAATTGGCGGAATATGCGGACTCCCTCGGAGCCCCCGAACTTCCGGGCAGCGGAAAACAGGAACAGCTCGAGAGCATAGTAAATCAGATACTTTTCGGATAA 727MI1_002Firmicutes Amino 48MIFENIPAIPYEGPKSTNPLAFKFYDPDKIVMGKPMKEHLPFAMAWWHNLGAAGTDMFGR AcidDTADKSFGAVKGTMEHAKAKVDAGFEFMQKLGIRYFCFHDVDLVPEADDIKETNRRLDEISDYILEKMKGTDIKCLWGTANMFSNPRFMNGAGSSNSADVFAFAAAQAKKALDLTVKLGGRGYVFWGGREGYETLLNTDMKFEQENIAKLMHMAVDYGRSIGFTGDFYIEPKPKEPMKHQYDFDAATAIGFLRQYGLDKDFKLNIEANHATLAGHTFQHDLRVSAINGMLGSIDANQGDMLLGWDTDEFPFNVYDATMCMYEVLKSDGLTGGFNFDSKSRRPSYTVEDMFTSYILGMDTFALGLLKAAELIEDGRLDAFVKERYSSYESGIGAKIRSGETDLKELAEYADSLGAPELPGSGKQEQLESIVNQILFG 727MI9_005 Firmicutes DNA 49ATGAGCGAGTTTTTTGCCAGCATTCCCAAAATTCCCTTTGAAGGCAAGGACAGCGCCAATCCCCTGGCGTTCAAATACTACGACGCCGACAGGATGATACTGGGCAAGCCCATGAAGGAGCACCTTCCCTTCGCCATGGCCTGGTGGCACAACCTGTGCGCCGCGGGCACCGATATGTTTGGCCGGGACACCGCCGACAAGTCCTTCGGCCAGGTCAAGGGCACCATGGAACACGCCAAGGCCAAGGTGGACGCGGGCTTTGAGTTCATGAAGAAGCTGGGCATCCGCTACTTCTGCTTCCACGACGTGGACATCGTGCCCGAAGCCGACGACATCAAGGAAACCAACCGCCGTCTGGACGAGATCTCCGACTATATCCTGGAGAAAATGAAAGGCACCGACATCCAGTGCCTGTGGGGCACCGCCAACATGTTCGGCAACCCCCGCTATATGAACGGCGCGGGCAGCTCCAACTCCGCCGACGTATACTGCTTCGCCGCGGCCCAGATCAAAAAGGCCCTGGACATCACCGTGAAGCTGGGCGGCAAGGGCTACGTGTTCTGGGGCGGCCGCGAGGGCTACGAGACCCTGCTGAACACCGATATGAAGTTCGAGCAGGAGAACATCGCCCGCCTGATGCACATGGCCGTGGACTACGGCCGCAGCATCGGCTTCACCGGCGATTTCTACATCGAGCCCAAGCCCAAGGAGCCCATGAAGCACCAGTACGACTTCGACGCCGCCACCGCCATAGGCTTTTTGCGCCAGTACGGCCTGGACAAGGATTTCAAGCTGAACATCGAGTCCAACCACGCCACCCTGGCGGGCCATACCTTCCAGCACGACCTGCGCGTTTCCGCCATCAACGGCATGCTGGGCTCCATCGACGCCAACCAGGGCGACTACCTGCTGGGCTGGGATACCGACGAGTTCCCCTACAGCGTATACGAGACCACCATGTGCATGTACGAGGTGCTCAAGGCCGGAGGTCTCACCGGCGGCTTCAATTTCGACGCCAAGAACCGCCGTCCCAGCTACACCCCCGAGGATATGTTCCACGCCTACATCCTTGGGATGGACAGCTTCGCCCTGGGCCTGATCAAGGCCGCCGAGCTCATCGAGGACGGTCGCCTGGACGCCTTCGTCCGGGACCGCTACCAGAGCTGGGAGACCGGCATCGGCGATAAGATCCGCAAGGGCGAGACCACACTGGCCGAGCTGGCCGAGTACGCCGCCCGGATGGGCGCGCCCGCGCTGCCCGGCAGCGGCCGCCAGGAATACCTGGAGGGCGTGGTCAACAATATCCTGTTCAAATAA 727MI9_005Firmicutes Amino 50MSEFFASIPKIPFEGKDSANPLAFKYYDADRMILGKPMKEHLPFAMAWWHNLCAAGTDMF AcidGRDTADKSFGQVKGTMEHAKAKVDAGFEFMKKLGIRYFCFHDVDIVPEADDIKETNRRLDEISDYILEKMKGTDIQCLWGTANMFGNPRYMNGAGSSNSADVYCFAAAQIKKALDITVKLGGKGYVFWGGREGYETLLNTDMKFEQENIARLMHMAVDYGRSIGFTGDFYIEPKPKEPMKHQYDFDAATAIGFLRQYGLDKDFKLNIESNHATLAGHTFQHDLRVSAINGMLGSIDANQGDYLLGWDTDEFPYSVYETTMCMYEVLKAGGLTGGFNFDAKNRRPSYTPEDMFHAYILGMDSFALGLIKAAELIEDGRLDAFVRDRYQSWETGIGDKIRKGETTLAELAEYAARMGAPALPGSGRQEYLEGVVNNILFK 727MI27_002 Firmicutes DNA 51ATGAAGACCTATTTCAAAAAAATCCCCGTGATCCCCTACGAGGGACCGAAGTCCCAGAATCCGCTGTCGTTCAAATTCTATGACGCGGACCGCATCGTTCTCGGCAAGCCCATGAAGGAGCATCTGCCCTTCGCCATGGCCTGGTGGCACAATCTGGGTGCTGCCGGAACGGACATGTTCGGCCGCGATACCGCCGACAAGTCCTTCGGAGCGGAGAAGGGCACCATGGAGCATGCCAAGGCCAAGGTGGACGCTGGCTTCGAGTTTATGAAGAAGGTGGGCATCCGGTATTTCTGCTTCCATGACGTGGATCTGGTCCCGGAAGCGGACGACATCAAGGAGACCAACCGCCGTCTCGATGAGATCAGCGACTACATCCTCAAGAAGATGAAGGGCACGGATATCAAGTGCCTCTGGGGCACCGCCAACATGTTCGGCAATCCCCGGTTCATGAACGGCGCGGGCAGCTCCAACAGCGCGGACGTGTTCTGCTTTGCCGCGGCCCAGGTGAAGAAGGCCTTGGACATCACCGTCAAGCTGGGCGGCCGGGGCTATGTGTTCTGGGGCGGCCGTGAGGGGTATGAGTCCCTGCTGAACACGGACGTGAAGTTTGAGCAGGAGAACATCGCCAAGCTCATGCACCTTGCCGTGGACTACGGCCGCAGCATCGGCTTCACCGGCGATTTCTACATCGAGCCCAAGCCCAAGGAGCCCATGAAGCACCAGTACGACTTCGATGCCGCCACCGCCATCGGCTTCCTCAGGCAGTACGGCCTCGATAAGGACTTCAAGATGAACATTGAAGCCAACCACGCGACCCTGGCCGGCCACACCTTCCAGCACGACCTCAGGATCAGCGCCATCAACGGGATGCTGGGCTCCATCGACGCCAACCAGGGCGACCTCCTGCTGGGATGGGACACCGACGAATTCCCCTTCAACGTCTATGAGGCCACCATGTGCATGTACGAGGTCCTCAAGGCCGGCGGCCTCACCGGCGGCTTCAACTTCGACTCAAAGAACCGCCGTCCCTCCTACACCATGGAGGATATGTTCCACGCCTACATCCTGGGCATGGACACCTTCGCCCTGGGTCTTCTCAAGGCCGCGGAGCTCATCGAGGACGGTCGGATCGACAAATTCGTGGAGGAGCGCTACGCCAGCTACAAGACCGGCATCGGCGCCAAGATCCGTTCCGGCGAGACCACGCTTCAGGAGCTGGCCGCCTATGCCGACAAGTTGGGCGCGCCTGCCCTTCCCGGCAGCGGCCGTCAGGAGTACCTGGAGAGCATCGTCAACCAGGTGCTCTTCGGGATGTGA 727MI27_002Firmicutes Amino 52MKTYFKKIPVIPYEGPKSQNPLSFKFYDADRIVLGKPMKEHLPFAMAWWHNLGAAGTDMF AcidGRDTADKSFGAEKGTMEHAKAKVDAGFEFMKKVGIRYFCFHDVDLVPEADDIKETNRRLDEISDYILKKMKGTDIKCLWGTANMFGNPRFMNGAGSSNSADVFCFAAAQVKKALDITVKLGGRGYVFWGGREGYESLLNTDVKFEQENIAKLMHLAVDYGRSIGFTGDFYIEPKPKEPMKHQYDFDAATAIGFLRQYGLDKDFKMNIEANHATLAGHTFQHDLRISAINGMLGSIDANQGDLLLGWDTDEFPFNVYEATMCMYEVLKAGGLTGGFNFDSKNRRPSYTMEDMFHAYILGMDTFALGLLKAAELIEDGRIDKFVEERYASYKTGIGAKIRSGETTLQELAAYADKLGAPALPGSGRQEYLESIVNQVLFGM 1753MI2_006 Neocallimastigales DNA 53ATGGCTAAAGAGTATTTTCCAGAGATTGGCAAAATCAAGTTTGAAGGCAAGGACAGCAAAAACCCAATGGCTTTCCACTACTATGACCCCGAGAAGGTGATCATGGGCAAGCCTATGAAAGACTGGCTCCGCTTCGCTATGGCATGGTGGCACACCCTCTGCGCAGAAGGTGGCGACCAGTTCGGTGGCGGCACTAAGAAGTTCCCTTGGAACAACGGCGCTGACGCTGTAGAAATCGCAAAACAGAAGGCTGACGCAGGTTTCGAAATCATGCAGAAGCTCGGCATCCCATATTTCTGCTTCCACGACGTGGACCTCGTGTCTGAGGGCGCATCTGTAGAAGAGTATGAGGCTAACCTCAAGGCTATCACAGACTACCTCGCTGTGAAGATGAAGGAAACAGGCATCAAGCTCCTGTGGTCTACTGCCAACGTATTCGGCAACGGCCGCTACATGAACGGTGCTTCTACCAACCCTGACTTCGACGTCGTTGCTCGCGCTATCGTGCAGATTAAGAACGCTATCGACGCTGGTATCAAGCTCGGCGCTGAGAACTACGTGTTCTGGGGCGGACGCGAAGGCTACATGAGCCTCCTCAACACCGACCAGAAGCGTGAGAAGGAGCACATGGCCACTATGCTCACTATGGCTCGCGACTACGCTCGCGCTAAGGGCTTCAAGGGCACATTCCTCATCGAGCCTAAGCCAATGGAGCCTTCTAAGCACCAGTATGACGTTGACACTGAGACTGTCATCGGCTTCCTCAAGGCACACAACCTCGACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCAACTCTCGCTGGCCACACCTTCGAGCACGAGCTCGCAGTGGCAGTGGACAACAACATGCTCGGCTCTATCGACGCTAACCGTGGTGACTACCAGAATGGCTGGGATACTGACCAGTTCCCAATCGACCAGTACGAACTCGTTCAGGCTTGGATGGAAATCATCCGTGGCGGCGGTCTCGGCACTGGCGGCACGAACTTCGACGCTAAGACTCGTCGTAACTCTACCGACCTCGAAGACATCTTCATCGCACACATCGCAGGCATGGACGCTATGGCACGCGCACTCGAATCAGCTGCTAAGCTCCTCGAAGAGTCTCCATACAAGGCAATGAAGGCAGCTCGCTACGCTTCATTCGACAACGGTATCGGTAAGGACTTCGAAGATGGCAAGCTCACTCTCGAGCAGGCTTACGAATACGGTAAGAAGGTTGGTGAGCCTAAGCAGACTTCTGGCAAGCAGGAGCTCTACGAAGCCATCGTTGCAATGTACGCTTAA 1753MI2_006Neocallimastigales Amino 54MAKEYFPEIGKIKFEGKDSKNPMAFHYYDPEKVIMGKPMKDWLRFAMAWWHTLCAEGGDQ AcidFGGGTKKFPWNNGADAVEIAKQKADAGFEIMQKLGIPYFCFHDVDLVSEGASVEEYEANLKATTDYLAVKMKETGIKLLWSTANVFGNGRYMNGASTNPDFDVVARAIVQIKNAIDAGIKLGAENYVFWGGREGYMSLLNTDQKREKEHMATMLTMARDYARAKGFKGTFLIEPKPMEPSKHQYDVDTETVIGFLKAHNLDKDFKVNIEVNHATLAGHTFEHELAVAVDNNMLGSIDANRGDYQNGWDTDQFPIDQYELVQAWMEIIRGGGLGTGGTNFDAKTRRNSTDLEDIFIAHIAGMDAMARALESAAKLLEESPYKAMKAARYASFDNGIGKDFEDGKLTLEQAYEYGKKVGEPKQTSGKQELYEAIVAMYA 5586MI3_005 Neocallimastigales DNA 55ATGGCTAAAGAATTTTTCCCAGAGATTGGTAAAATCAAGTTCGAAGGCAAGGATTCAAAGAATCCAATGGCTTTCCATTACTATGATGCAGAGAAGGTAATCATGGGCAAACCCATGAAGGACTGGCTCCGTTTCGCTATGGCATGGTGGCACACACTCTGTGCAGAGGGCGGCGACCAGTTCGGTGGCGGTACGAAGAAGTTCCCTTGGAACGAGGGTGCTAATGCTGTCGAGATTGCTAAGCAGAAGGCTGACGCTGGTTTCGAAATCATGCAGAAGCTTGGCATTCCTTACTTCTGCTTCCACGATGTTGACCTCGTTTCTGAAGGCGCATCTGTTGAGGAGTATGAGGCCAACCTCAAGGCTATCACTGACTATCTCGCGGTGAAGATGAAGGAGACTGGCATTAAGCTCCTGTGGTCTACTGCCAACGTGTTCGGCAATGGCCGTTACATGAATGGTGCTTCCACCAACCCTGACTTCGACGTTGTTGCTCGCGCCATCGTTCAGATTAAGAACGCTATCGATGCAGGTATCAAGCTCGGTGCTGAGAACTATGTGTTCTGGGGCGGTCGTGAAGGTTACATGAGCCTCCTGAACACAGACCAGAAGCGTGAGAAGGAGCACATGGCTACTATGCTCACTATGGCTCGCGACTACGCTCGCAGCAAGGGCTTCAAGGGTACTTTCCTCATCGAGCCTAAGCCAATGGAGCCATCTAAGCACCAGTACGACGTTGACACAGAGACTGTTATCGGCTTCCTGAAGGCACACAACCTTGACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCAACACTCGCTGGTCACACCTTCGAGCACGAGCTCGCTGTGGCTGTCGACAACAATATGCTTGGTTCTATCGATGCTAACCGCGGTGACTACCAGAATGGTTGGGATACGGACCAGTTCCCAATTGACCAGTACGAGCTCGTTCAGGCTTGGATGGAGATCATCCGTGGTGGCGGTCTCGGCACAGGTGGTACAAACTTCGACGCTAAGACTCGTCGTAACTCTACCGACCTCGAGGACATTTTCATTGCTCACATCGCTGGTATGGACGCTATGGCTCGCGCTCTTGAGTCAGCAGCTAAGCTCCTTGAGGAGTCTCCATACAAGAAGATGAAGGCTGCCCGTTATGCTTCTTTCGACAGCGGCATGGGTAAGGACTTTGAGAACGGCAAGCTCACACTCGAACAGGTTTATGAGTATGGTAAGAAGGTAGGTGAGCCCAAGCAGACTTCTGGCAAGCAGGAGCTCTTCGAGGCAATCGTGGCCATGTACGCATAA 5586MI3_005Neocallimastigales Amino 56MAKEFFPEIGKIKFEGKDSKNPMAFHYYDAEKVIMGKPMKDWLRFAMAWWHTLCAEGGDQ AcidFGGGTKKFPWNEGANAVEIAKQKADAGFEIMQKLGIPYFCFHDVDLVSEGASVEEYEANLKAITDYLAVKMKETGIKLLWSTANVFGNGRYMNGASTNPDFDVVARAIVQIKNAIDAGIKLGAENYVFWGGREGYMSLLNTDQKREKEHMATMLTMARDYARSKGFKGTFLIEPKPMEPSKHQYDVDTETVIGFLKAHNLDKDFKVNIEVNHATLAGHTFEHELAVAVDNNMLGSIDANRGDYQNGWDTDQFPIDQYELVQAWMEIIRGGGLGTGGTNFDAKTRRNSTDLEDIFIAHIAGMDAMARALESAAKLLEESPYKKMKAARYASFDSGMGKDFENGKLTLEQVYEYGKKVGEPKQTSGKQELFEAIVAMYA 5586MI91_002 Neocallimastigales DNA 57ATGGCTAAAGAGTATTTTCCAGAGATTGGTAAAATCAAGTTTGAAGGCAAGGATTCCAAGAATCCAATGGCATTCCACTATTATGATGCAGAGAAAGTGATTATGGGTAAGCCTATGAAGGAGTGGCTCCGCTTTGCAATGGCATGGTGGCACACACTCTGTGCAGAGGGTGGCGACCAGTTTGGTGGTGGCACTAAGAAATTCCCATGGAACGAGGGCACTGACGCTGTGACGATTGCTAAGCAGAAGGCTGATGCAGGTTTCGAAATCATGCAGAAACTCGGTTTCCCATATTTTTGCTTCCACGACATTGACCTCGTTTCCGAAGGCAACAGCATTGAAGAGTATGAGGCTAACCTCCAGGCAATCACTGATTATCTGAAAGTGAAGATGGAAGAGACAGGCATCAAACTCTTGTGGTCAACTGCCAACGTATTCGGCAATGGTCGCTACATGAATGGTGCTTCCACAAACCCAGACTTTGACGTGGTGGCTCGTGCCATCGTTCAGATTAAGAACGCAATTGACGCTGGTATCAAACTCGGTGCTGAGAACTATGTATTCTGGGGCGGTCGCGAAGGCTACATGAGCCTTCTGAACACTGACCAGAAGCGTGAGAAGGAGCACATGGCAACCATGCTCACTATGGCTCGCGACTACGCTCGCAGCAAGGGTTTCAAGGGCACTTTCCTCATTGAGCCAAAGCCAATGGAGCCATCTAAGCACCAGTATGACGTTGACACGGAGACTGTCATCGGCTTCCTCAAGGCACACAACCTCGACAAGGATTTCAAGGTGAACATCGAAGTGAACCACGCTACACTTGCAGGTCATACTTTCGAGCACGAACTTGCTGTGGCTGTTGACAATGGCATGCTCGGTTCTATCGACGCTAACCGTGGTGACTATCAGAACGGTTGGGACACTGACCAGTTCCCAATCGACCAGTACGAACTCGTTCAGGCTTGGATGGAAATCATCCGTGGTGGTGGTCTCGGCACAGGTGGTACTAACTTCGATGCTAAGACTCGTCGTAACTCAACTGACCTCGAGGACATCTTCATCGCACACATCTCTGGTATGGATGCAATGGCACGTGCTCTCGAATCGGCGGCTAAACTTCTTGAGGAGTCTCCATACTGCGCTATGAAGAAGGCTCGTTACGCTTCCTTCGACAGCGGCATCGGTAAGGACTTCGAGGACGGCAAACTCACGCTCGAGCAGGCTTACGAGTACGGCAAGAAAGTCGGCGAACCCAAGCAGACTTCTGGCAAGCAGGAACTCTACGAGGCAATCGTTGCCATGTACGCATAA 5586MI91_002Neocallimastigales Amino 58MAKEYFPEIGKIKFEGKDSKNEMAFHYYDAEKVIMGKPMKEWLRFAMAWWHTLCAEGGDQ AcidFGGGTKKFPWNEGTDAVTIAKQKADAGFEIMQKLGFPYFCFHDIDLVSEGNSIEEYEANLQAITDYLKVKMEETGIKLLWSTANVFGNGRYMNGASTNPDFDVVARAIVQIKNAIDAGIKLGAENYVFWGGREGYMSLLNTDQKREKEHMATMLTMARDYARSKGFKGTFLIEPKPMEPSKHQYDVDTETVIGFLKAHNLDKDFKVNIEVNHATLAGHTFEHELAVAVDNGMLGSIDANRGDYQNGWDTDQFPIDQYELVQAWMEIIRGGGLGTGGTNFDAKTRRNSTDLEDIFIAHISGMDAMARALESAAKLLEESPYCAMKKARYASFDSGIGKDFEDGKLTLEQAYEYGKKVGEPKQTSGKQELYEAIVAMYA 5586MI194_003 Neocallimastigales DNA 59ATGGCAAAAGAGTATTTCCCTACGATCGGTAAGATCGTTTATGAAGGACCGGAGTCCAAGAACCCTATGGCATTTCATTACTATGACGCAGAGCGCGTAGTAGCTGGTAAAAAAATGAAAGATTGGATGCGTTTCGCTATGGCATGGTGGCACACCCTCTGTGCAGAAGGTGCAGACCAGTTCGGTGGAGGCACCAAACACTTCCCGTGGAGTGAAGGTCCCGATGCCGTAACCATCGCCAAGCAGAAAGCAGACGCAGGTTTTGAGATCATGCAGAAACTCGGCTTCCCGTATTTCTGTTTCCATGACGTGGATCTGGTCAGCGAAGGCAGCAGCGTAGAAGAGTACGAGGCGAACCTCGCAGCCATCACCGATTATCTCAAGCAGAAAATGGACGAGTCGGGTATCAAACTCCTTTGGTCCACTGCTAACGTATTCGGTCACGCCCGTTACATGAACGGTGCCAGCACCAATCCTGACTTTGATGTCGTTGCCCGTGCGATTGTGCAGATCAAGAATGCTATCGACGCAGGTATCAAACTCGGCGCAGAGAACTACGTCTTCTGGGGCGGTCGTGAAGGTTATATGAGCCTGCTCAATACCGACCAGAAACGCGAGAAAGAGCATACGGCAATGATGCTGCGTATGGCGCGTGACTATGCCCGCAGCAAAGGTTTCAAAGGTACCTTCCTCATCGAACCCAAACCCATGGAGCCGTCCAAGCACCAGTATGACGTAGATACCGAGACGGTGATAGGTTTCCTCAAAGCACACGGTTTGGAGAAAGACTTTAAGGTAAACATCGAAGTGAACCACGCTACCCTCGCCGGTCACACTTTCGAGCACGAACTGGCAGTAGCCGTAGATAACGGCATGCTCGGTTCGATCGATGCCAACCGCGGTGACTATCAGAACGGATGGGATACCGACCAGTTCCCCATCGATAACTTCGAACTGACCCAAGCATGGATGCAGATCGTACGTAACGGTGGTCTCGGCACAGGCGGAACGAACTTCGACTCCAAGACCCGTCGTAACTCCACCGATCTCGAGGATATCTTCATCGCTCACATCAGTGGTATGGACGCTTGTGCCCGTGCCCTATTGAATGCCGTAGAGATCATGGAGAAATCACCGATCCCTGCTATGCTCAAAGAGCGTTACGCTTCCTTCGATAGCGGTCTGGGTAAAGATTTCGAGGACGGCAAACTGACCCTTGAGCAAGTCTATGAGTACGGTAAGAAAGTAGGCGAACCCAAACAAACCAGCGGCAAACAAGAACTCTATGAGGCTATCGTTGCCCTCTACGCTAAATAA 5586MI194_003Neocallimastigales Amino 60MAKEYFPTIGKIVYEGPESKNPMAFHYYDAERVVAGKKMKDWMRFAMAWWHTLCAEGADQ AcidFGGGTKHFPWSEGPDAVTIAKQKADAGFEIMQKLGFPYFCFHDVDLVSEGSSVEEYEANLAAITDYLKQKMDESGIKLLWSTANVFGHARYMNGASTNPDFDVVARAIVQIKNAIDAGIKLGAENYVFWGGREGYMSLLNTDQKREKEHTAMMLRMARDYARSKGFKGTFLIEPKPMEPSKHQYDVDTETVIGFLKAHGLEKDFKVNIEVNHATLAGHTFEHELAVAVDNGMLGSIDANRGDYQNGWDTDQFPIDNFELTQAWMQIVRNGGLGTGGTNFDSKTRRNSTDLEDIFIAHISGMDACARALLNAVEIMEKSPIPAMLKERYASFDSGLGKDFEDGKLTLEQVYEYGKKVGEPKQTSGKQELYEAIVALYAK 5586MI198_003 Neocallimastigales DNA 61ATGAAAGAGTATTTCCCTGAGATCGGTAAGATCCAATTTGAAGGCCCGGAGTCCAAGAACCCGATGGCATTTCACTACTATGACGCAGAGCGCGTCGTAGCCGGTAAAACAATGAAAGAGTGGATGCGTTTCGCTATGGCTTGGTGGCACACCCTCTGTGCGGAAGGCGGCGACCAGTTCGGAGGCGGAACGAAGAAGTTCCCCTGGAACGAAGGCGCTAACGCTTTGGAGATCGCCAAGCACAAAGCCGATGCGGGATTTGAGATCATGCAGAAACTCGGCATCCCTTATTTCTGTTTCCATGACGTGGATCTCATCGCCGAGGGCGGTTCGGTAGAAGAGTACGAAGCCAACCTCGCTGCCATCACCGATTACCTCAAACAGAAAATGGACGAGACTGGCATCAAACTGCTGTGGTCCACGGCGAACGTCTTCAGCAACCCCCGTTATATGAACGGCGCCAGCACGAACCCCGATTTCGATGTAGTAGCGCGTGCCATCGTCCAGATCAAGAACGCTATCGACGCCGGTATCAAACTCGGAGCAGAGAACTATGTCTTCTGGGGTGGTCGCGAGGGCTATATGAGCCTCCTCAACACTGACCAGCGCCGAGAGAAAGAGCATATGGCTACCATGCTCCGTATGGCGCGTGACTACGCGCGTGCCAAAGGATTCAAGGGCACCTTCCTCATCGAACCCAAACCATGTGAGCCGTCCAAACATCAGTATGATGTCGATACCGAGACCGTCATCGGTTTCCTCAAAGCGCATGGACTCGACAAGGATTTCAAAGTCAATATCGAGGTCAACCACGCCACCCTCGCAGGCCACACGTTCGAACACGAACTGGCTTGCGCTGTAGATGCCGGCATGCTCGGTTCGATTGACGCCAACCGCGGTGACGCCCAGAACGGATGGGACACCGACCAGTTCCCTATTGATAACTTCGAACTCACACAGGCTTTCATGCAGATCGTCCGCAACGGCGGTTTCGGAACAGGCGGTACGAACTTCGACGCCAAGACACGCCGTAACTCCACCGACTTGGAGGACATCTTCATCGCCCATATCAGCGGCATGGACGCTTGCGCACGTGCGTTACTCAATGCTGTCGAAATCCTCGAGAAGAGCCCGATTCCGGCGATGCTCAAAGAGCGTTATGCTTCCTTTGACGGCGGCATCGGAAAGGACTTCGAGGAGGGAAAACTGACTTTCGAGCAGGTCTATGAGTACGGCAAGAAAGTCGGCGAACCCAAACAGACCAGCGGCAAACAGGAGCTCTACGAAACCATCGTCGCCCTCTATGCCAAATAG 5586MI198_003Neocallimastigales Amino 62MKEYFPEIGKIQFEGPESKNPMAFHYYDAERVVAGKTMKEWMRFAMAWWHTLCAEGGDQF AcidGGGTKKFPWNEGANALEIAKHKADAGFEIMQKLGIPYFCFHDVDLIAEGGSVEEYEANLAAITDYLKQKMDETGIKLLWSTANVFSNPRYMNGASTNPDFDVVARAIVQIKNAIDAGIKLGAENYVFWGGREGYMSLLNTDQRREKEHMATMLRMARDYARAKGFKGTFLIEPKPCEPSKHQYDVDTETVIGFLKAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANRGDAQNGWDTDQFPIDNFELTQAFMQIVRNGGEGTGGTNEDAKTRRNSTDLEDIFIAHISGMDACARALLNAVEILEKSPIPAMLKERYASFDGGIGKDFEEGKLTFEQVYEYGKKVGEPKQTSGKQELYETIVALYAK 5586MI201_003 Neocallimastigales DNA 63ATGGCAAAAGAGTATTTCCCTACGATCGGTAAGATCGTTTATGAAGGACCGGAATCCAAGAACCCTATGGCATTTCATTACTATGACGCAGAGCGCGTAGTAGCTGGTAAAAAAATGAAAGATTGGATGCGTTTCGCTATGGCATGGTGGCACACCCTCTGTGCAGAAGGTGCAGACCAGTTCGGTGGAGGCACCAAACACTTCCCGTGGAATGAAGGTCCCGATGCCGTAACCATCGCCAAGCAGAAAGCAGACGCAGGTTTTGAGATCATGCAGAAACTCGGCTTCCCGTATTTCTGTTTCCATGACGTGGATCTGGTCGGCGAAGGCAGCAGCGTAGAAGAGTACGAGGCGAACCTCGCAGCCATCACCGATTATCTCAAGCAGAAAATGGACGAGTCGGGTATCAAACTCCTTTGGTCCACTGCTAACGTATTCGGTCACGCCCGTTACATGAACGGTGCCAGCACCAATCCTGACTTTGATGTCGTTGCCCGTGCGATTGTGCAGATCAAGAATGCTATCGACGCAGGTATCAAACTCGGCGCAGAGAACTACGTCTTCTGGGGCGGTCGTGAAGGTTATATGAGCCTGCTCAACACCGACCAGAAACGCGAGAAAGAGCATACGGCAATGATGCTGCGTATGGCGCGTGACTATGCCCGCAGCAAAGGTTTCAAAGGTACCTTCCTCATCGAACCCAAACCCATGGAGCCGTCCAAGCACCAGTATGACGTAGATACCGAGACGGTGATAGGTTTCCTCAAAGCACACGGTTTGGAGAAAGACTTTAAGGTAAACATCGAAGTGAACCACGCTACCCTCGCCGGTCACACTTTCGAGCACGAACTGGCAGTAGCCGTAGATAACGGCATGCTCGGTTCGATCGATGCCAACCGCGGTGACTATCAGAACGGATGGGATACCGACCAGTTCCCCATCGATAACTTCGAACTGACCCAAGCATGGATGCAGATCGTACGTAACGGTGGTCTCGGCACAGGCGGAACGAACTTCGACTCCAAGACCCGTCGTAACTCCACCGATCTCGAGGATATCTTCATCGCTCACATCAGTGGTATGGACGCTTGTGCCCGTGCCCTATTGAATGCCGTAGAGATCATGGAGAAATCACCGATCCCTGCTATGCTCAAAGAGCGTTACGCTTCCTTCGATAGCGGTCTGGGTAAAGATTTCGAGGACGGCAAACTGACCCTTGAGCAAGTCTATGAGTACGGTAAGAAAGTAGGCGAACCCAAACAAACCAGCGGCAAACAAGAACTCTATGAGGCTATCGTTGCCCTCTACGCTAAATAA 5586MI201_003Neocallimastigales Amino 64MAKEYFPTIGKIVYEGPESKNPMAFHYYDAERVVAGKKMKDWMRFAMAWWHTLCAEGADQ AcidFGGGTKHFPWNEGPDAVTIAKQKADAGFEIMQKLGFPYFCFHDVDLVGEGSSVEEYEANLAAITDYLKQKMDESGIKLLWSTANVFGHARYMNGASTNPDFDVVARAIVQIKNAIDAGIKLGAENYVFWGGREGYMSLLNTDQKREKEHTAMMLRMARDYARSKGFKGTFLIEPKPMEPSKHQYDVDTETVIGFLKAHGLEKDFKVNIEVNHATLAGHTFEHELAVAVDNGMLGSIDANRGDYQNGWDTDQFPIDNFELTQAWMQIVRNGGLGTGGTNFDSKTRRNSTDLEDIFIAHISGMDACARALLNAVEIMEKSPIPAMLKERYASFDSGLGKDFEDGKLTLEQVYEYGKKVGEPKQTSGKQELYEAIVALYAK 5586MI204_002 Neocallimastigales DNA 65ATGAAAGAGTATTTCCCTGAGGTCGGTAAGATCCAATTTGAAGGCCCGGAGTCTAAGAACCCGATGGCATTTCACTACTATGACGCAGAGCGCGTCGTAGCCGGTAAAACAATGAAAGAGTGGATGCGTTTCGCTATGGCTTGGTGGCACACCCTCTGTGCAGAAGGCGGCGACCAGTTCGGAGGCGGAACGAAGCATTTCCCGTGGAATGAAGGCGCTAACGCTTTGGAGATCGCCAAACACAAAGCCGATGCGGGATTCGAGATCATGCAGAAACTCGGCATCCCCTATTTCTGTTTCCATGACGTGGATCTCATCGCCGAGGGCGGTTCGGTAGAAGAGTACGAAACCAACCTCGCTGCTATCACCGACTACCTCAAGCAGAAAATGGACGAGACCGGCATCAAACTGCTGTGGTCCACGGCGAACGTGTTCAGCAACCCCCGTTATATGAACGGCGCGAGCACGAACCCCGATTTCGATGTAGTAGCGCGTGCCATCGTGCAGATCAAGAATGCCATCGACGCCGGCATCAAACTGGGCGCAGAGAACTATGTCTTCTGGGGCGGTCGCGAGGGCTACATGAGCCTGCTCAACACCGACCAGCGCCGCGAGAAAGAGCATATGGCTACTATGCTCCGTATGGCGCGTGACTACGCGCGTGCCAAAGGATTCAAGGGCACCTTTCTCATCGAACCCAAACCGTGTGAGCCGTCCAAACATCAGTATGATGTCGATACCGAGACCGTCATCGGTTTCCTCAAAGCGCATGGACTCGACAAGGATTTCAAGGTTAATATCGAGGTCAACCACGCCACCCTCGCAGGCCACACGTTCGAACACGAACTGGCTTGCGCTGTAGATGCCGGCATGCTCGGTTCGATTGACGCCAACCGCGGTGACGCCCAGAACGGATGGGACACCGACCAGTTCCCTATTGATAACTTCGAACTCACACAGGCTTTCATGCAGATCGTCCGCAACGGCGGTTTCGGAACAGGCGGTACGAACTTCGACGCCAAGACACGCCGTAACTCCACCGACTTGGAGGACATCTTCATCGCCCATATCAGCGGCATGGACGCTTGCGCACGTGCGTTGCTCAACGCCATCGAAATCCTCGAGAAGAGCCCGATCCCGGCTATGCTCAAAGACCGTTATGCCTCCTTTGATGGCGGCATCGGAAAGGACTTTGAGGAGGGCAAACTGACTTTCGAGCAGGTCTATGAGTACGGCAAGAAGGTCGGAGAACCCAAACAGACCAGCGGCAAACAGGAGCTCTACGAAACCATCGTCGCCCTCTATGCCAAATAG 5586MI204_002Neocallimastigales Amino 66MKEYFPEVGKIQFEGPESKNPMAFHYYDAERVVAGKTMKEWMRFAMAWWHTLCAEGGDQF AcidGGGTKHFPWNEGANALEIAKHKADAGFEIMQKLGIPYFCFHDVDLIAEGGSVEEYETNLAAITDYLKQKMDETGIKLLWSTANVFSNPRYMNGASTNPDFDVVARAIVQIKNAIDAGIKLGAENYVFWGGREGYMSLLNTDQRREKEHMATMLRMARDYARAKGFKGTFLIEPKPCEPSKHQYDVDTETVIGFLKAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANRGDAQNGWDTDQFPIDNFELTQAFMQIVRNGGFGTGGTNFDAKTRRNSTDLEDIFIAHISGMDACARALLNAIEILEKSPIPAMLKDRYASFDGGIGKDFEEGKLTFEQVYEYGKKVGEPKQTSGKQELYETIVALYAK 5586MI207_002 Neocallimastigales DNA 67ATGAAAGAGTATTTCCCTGAGATCGGTAAGATGCAATTTGAAGGCCCGGAGTCCAAGAACCCGATGGCGTTTCACTACTATGACGCTGAGCGCGTCGTAGCCGGTAAAACAATGAAAGAGTGGATGCGTTTCGCTATGGCTTGGTGGCACACCCTCTGTGCGGAAGGCGGCGACCAGTTCGGAGGAGGAACGAAGAAATTCCCCTGGAACGAAGGGGCAAACGCTTTGGAGATCGCCAAGCACAAAGCCGATGCGGGATTCGAGATCATGCAGAAACTCGGCATCCCTTATTTCTGTTTCCATGACGTGGATCTCATCGCCGAGGGCGAATCGGTAGAAGAGTACGAAGCCAACCTCGCTGCCATCACCGATTACCTCAAACAGAAAATGGACGAGACCGGCATCAAACTGCTGTGGTCCACGGCGAACGTGTTCAGCAACCCCCGTTATATGAACGGCGCCAGCACGAACCCCGATTTCGATGTAGTGGCACGCGCTATCGTACAAATCAAGAACGCTATCGACGCCGGTATCAAACTCGGAGCAGAGAACTATGTCTTCTGGGGCGGTCGCGAGGGCTATATGTCGCTCCTCAACACCGACCAGCGCCGAGAGAAAGAGCATATGGCTACTATGCTCCGTATGGCGCGTGACTACGCGCGTTCCAAAGGATTCAAGGGCACCTTCCTCATCGAACCCAAACCGTGTGAGCCGTCCAAACATCAGTACGATGTGGACACAGAGACCGTCATCGGTTTCCTTAAAGCGCATGGACTCGACAAGGATTTCAAAGTCAATATCGAGGTCAACCACGCCACCCTCGCAGGCCACACGTTCGAACACGAACTGGCTTGCGCTGTAGATGCCGGCATGCTCGGTTCGATTGACGCCAACCGCGGTGACGCCCAGAACGGATGGGACACCGACCAATTCCCTATTGATAACTTCGAACTCACTCAGGCTTTCATGCAGATCGTCCGCAACGGCGGTTTCGGAACAGGCGGTACGAACTTCGACGCCAAGACACGCCGTAACTCCACCGACTTGGAGGACATCTTCATCGCCCATATCAGCGGCATGGACGCTTGCGCTCGTGCGTTGCTCAATGCTGTCGAAATCCTCGAGAAGAGCCCGATCCCGGCTATGCTCAAAGAGCGTTATGCTTCCTTTGACGGCGGCATCGGAAAGGACTTTGAGGAGGGCAAACTGACTTTCGAGCAGGTCTATGAGTACGGCAAGAAGGTCGGAGAACCCAAACAGACCAGCGGCAAACAGGAGCTCTACGAAACCATCGTCGCCCTCTATGCCAAATGA 5586MI207_002Neocallimastigales Amino 68MKEYFPEIGKIQFEGPESKNPMAFHYYDAERVVAGKTMKEWMRFAMAWWHTLCAEGGDQF AcidGGGTKKFPWNEGANALEIAKHKADAGFEIMQKLGIPYFCFHDVDLIAEGESVEEYEANLAAITDYLKQKMDETGIKLLWSTANVFSNPRYMNGASTNPDFDVVARAIVQIKNAIDAGIKLGAENYVFWGGREGYMSLLNTDQRREKEHMATMLRMARDYARSKGFKGTFLIEPKPCEPSKHQYDVDTETVIGFLKAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANRGDAQNGWDTDQFPIDNFELTQAFMQIVRNGGFGTGGTNFDAKTRRNSTDLEDIFIAHISGMDACARALLNAVEILEKSPIPAMLKERYASFDGGIGKDFEEGKLTFEQVYEYGKKVGEPKQTSGKQELYETIVALYAK 5586MI209_003 Neocallimastigales DNA 69ATGAAAGAGTATTTCCCTGAGATCGGTAAGATCCAATTTGAAGGCCCGGAGTCCAAGAACCCGATGGCGTTTCACTACTATGACGCAGAGCGCGTAGTAGCCGGTAAAACAATGAAAGAATGGATGCGTTTCGCCATGGCATGGTGGCACACCCTCTGTGCAGAAGGCGGCGACCAGTTCGGAGGAGGAACGAAGCATTTCCCGTGGAATGAAGGCGCTAACGCTTTGGAGATCGCCAAACACAAAGCCGATGCGGGATTCGAGATCATGCAGAAACTCGGCATCCCCTATTTCTGTTTCCATGACGTGGATCTCATCGCCGAGGGCGATTCGGTGGAGGAGTACGAAGCTAACCCCGCTGCCATCACCGATTACCTCAAACAGAAAATGGACGAGACCGGCATCAAACTGCTGTGGTCCACGGCGAACGTCTTCAGCAACCCCCGTTACATGAACGGTGCGAGCACGAACCCGGATTTCGATGTAGTGGCACGCGCTATCGTACAAATCAAGAACGCTATCGACGCCGGTATCAAACTCGGAGCAGAGAACTATGTCTTCTGGGGCGGTCGCGAGGGCTATATGTCGCTCCTCAACACCGACCAGCGTCGCGAGAAAGAGCATATGGCTACTATGCTCCGTATGGCGCGTGACTACGCGCGTGCCAAAGGATTCAAGGGCACCTTCCTCATCGAACCCAAACCATGTGAGCCGTCCAAACATCAGTACGATGTGGACACAGAGACTGTCATCGGTTTCCTCAAAGCGCATGGACTCGACAAGGATTTCAAAGTCAACATCGAGGTCAACCACGCCACCCTCGCAGGTCACACGTTCGAACACGAACTGGCTTGCGCTGTAGATGCCGGCATGCTCGGTTCGATTGACGCCAACCGCGGTGACGCCCAGAACGGATGGGACACTGACCAGTTCCCTATTGATAACTTCGAACTCACACAGGCTTTCATGCAGATCGTCCGCAACGGCGGTTTCGGAACAGGCGGTACGAACTTCGACGCCAAGACACGCCGTAACTCCACCGACTTGGAGGACATCTTCATCGCCCATATCAGCGGCATGGACGCTTGTGTCCGTGCGTTGCTCAACGCCATCGAAATCCTCGAGAAGAGCCCGATCCCGGCTATGCTCAAAGAGCGTTACGCTTCCTTTGACGGCGGCATCGGAAAGGACTTTGAGGATGGTAAACTGACTTTCGAGCAGGTCTATGAGTACGGCAAGAAGGTCGGAGAACCCAAACAGACCAGCGGCAAACAGGAGCTCTACGAAACCATCGTCGCCCTCTATGCCAAGTAA 5586MI209_003Neocallimastigales Amino 70MKEYFPEIGKIQFEGPESKNPMAFHYYDAERVVAGKTMKEWMRFAMAWWHTLCAEGGDQF AcidGGGTKHFPWNEGANALEIAKHKADAGFEIMQKLGIPYFCFHDVDLIAEGDSVEEYEANPAAITDYLKQKMDETGIKLLWSTANVFSNPRYMNGASTNPDFDVVARAIVQIKNAIDAGIKLGAENYVFWGGREGYMSLLNTDQRREKEHMATMLRMARDYARAKGFKGTFLIEPKPCEPSKHQYDVDTETVIGFLKAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANRGDAQNGWDTDQFPIDNFELTQAFMQIVRNGGFGTGGTNFDAKTRRNSTDLEDIFIAHISGMDACVRALLNAIEILEKSPIPAMLKERYASFDGGIGKDFEDGKLTFEQVYEYGKKVGEPKQTSGKQELYETIVALYAK 5586MI214_002 Neocallimastigales DNA 71ATGAAAGAGTATTTCCCTGAGATCGGAAAGATCCAATTCGAAGGCCCGGAGTCCAAGAATCCTATGGCATTTCACTACTATGACGCAGAGCGTGTAGTAGCCGGTAAAACAATGAAAGAGTGGATGCGTTTCGCTTTGGCATGGTGGCACACGCTCTGCGCAGAAGGCGGCGACCAGTTCGGAGGCGGCACGAAGCATTTCCCTTGGAATGAAGGTGCAAACGCTTTGGAGATCGCCAAGCACAAAGCCGATGCAGGCTTCGAGATCATGCAGAAACTCGGCATCCCCTATTTCTGTTTCCATGACGTGGATCTGATCGCCGAGGGCGGTTCGGTAGAAGAGTATGAAGCTAATTTAACGGCTATCACCGATTACCTCAAACAGAAAATGGACGAGACCGGCATCAAACTGCTGTGGTCCACTGCGAACGTGTTCGGTAACGCACGTTATATGAACGGCGCGAGCACGAACCCCGATTTCGATGTAGTGGCACGCGCTATCGTGCAGATCAAGAACGCTATCGACGCCGGCATCAAACTGGGCGCAGAGAACTACGTCTTCTGGGGCGGTCGCGAGGGATATATGTCGCTCCTGAACACCGACCAGAAGCGTGAGAAAGAGCATATGGCTACCATGCTCCGTATGGCGCGTGACTACGCGCGTTCCAAAGGATTCAAAGGTACGTTCCTCATCGAGCCCAAACCGTGTGAGCCGTCCAAACATCAGTACGACGTGGACACTGAGACCGTCATCGGTTTCCTCAAAGCCCATGGTCTCGGCAAGGATTTCAAAGTGAACATCGAGGTGAATCACGCCACCCTCGCAGGGCACACGTTCGAACACGAACTGGCTTGCGCCGTAGATGCCGGCATGCTCGGTTCGATCGACGCCAACCGCGGTGACGCACAAAACGGATGGGACACCGACCAGTTCCCTATTGATAATTTCGAACTCACCCAGGCATTCATGCAGATCGTCCGCAACGGCGGTTTCGGAACAGGCGGTACGAACTTCGACGCCAAGACACGCCGTAATTCCACCGACTTGGAGGACATCTTCATCGCCCATATCAGCGGCATGGACGCTTGTGCCCGTGCGTTGCTCAATGCTGTCGAAATCCTTGAAAAGAGCCCGATCCCGGCGATGCTCAAAGAGCGTTACGCCTCCTTTGACAGCGGTATGGGTAAGGACTTTGAGGAGGGCAAGCTGACCTTCGAGCAGGTCTATGAGTACGGCAAACAGGTCGGCGAACCCAAACAGACCAGCGGCAAGCAGGAGCTCTACGAAACCATCGTCGCCCTCTATGCCAAATAG 5586MI214_002Neocallimastigales Amino 72MKEYFPEIGKIQFEGPESKNPMAFHYYDAERVVAGKTMKEWMRFALAWWHTLCAEGGDQF AcidGGGTKHFPWNEGANALEIAKHKADAGFEIMQKLGIPYFCFHDVDLIAEGGSVEEYEANLTAITDYLKQKMDETGIKLLWSTANVFGNARYMNGASTNPDFDVVARAIVQIKNAIDAGIKLGAENYVFWGGREGYMSLLNTDQKREKEHMATMLRMARDYARSKGFKGTFLIEPKPCEPSKHQYDVDTETVIGFLKAHGLGKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANRGDAQNGWDTDQFPIDNFELTQAFMQIVRNGGFGTGGTNFDAKTRRNSTDLEDIFIAHISGMDACARALLNAVEILEKSPIPAMLKERYASFDSGMGKDFEEGKLTFEQVYEYGKQVGEPKQTSGKQELYETIVALYAK 5751MI3_001 Neocallimastigales DNA 73ATGAAAGAGTATTTTCCACAAATCGGCAAGATCCCATTTGAGGGACCAGAGTCAAAGAACCCAATGGCATTCCACTACTATGACGCAGAGCGCGTAGTTGCCGGTAAGACAATGAAGGAATGGATGCGTTTCGCTATGGCCTGGTGGCACACTCTCTGTGCTGAGGGTAGCGATCAGTTCGGCCCTGGTACAAAGAAGTTCCCTTGGAACGAGGGCGAGACAGCCCTTGAGCGCGCTAAGCACAAGGCAGATGCTGGCTTCGAGGTTATGCAGAAGCTCGGCATCCCATATTTCTGCTTCCACGATGTAGACCTTATCGACGAGGGTGCTAACGTGGCTGAGTATGAGGCAAACCTCGCTGCTATCACTGACTACCTGAAGGAGAAGATGGAGGAGACTGGCGTAAAGCTCCTCTGGTCTACAGCCAACGTGTTCGGTAACGCTCGCTATATGAACGGTGCTTCTACAAATCCTGACTTCGACGTTGTGGCTCGTGCCATCGTACAGATTAAGAACGCTATCGACGCTGGTATCAAGCTTGGTGCTGAGAACTACGTGTTCTGGGGCGGCCGCGAGGGCTACATGAGCCTTCTGAACACTGACCAGAAGCGCGAGAAGGAGCACATGGCAACTATGCTCGGCATGGCTCGCGACTATGCCCGCGCTAAGGGATTCACCGGTACCTTCCTCATTGAGCCAAAGCCAATGGAGCCAACAAAGCATCAGTATGATGTTGACACAGAGACCGTTATCGGTTTCCTCAAGGCTCACGGTCTGGACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCTACTCTCGCCGGTCACACCTTCGAGCACGAGCTCGCTTGCGCTGTTGACGCTGGTATGCTCGGTTCTATCGACGCTAACCGCGGTGACGCTCAGAACGGATGGGATACCGACCAGTTCCCAATCGACAACTTCGAGCTGACACAGGCTTGGATGCAGATTGTTCGCAATGGCGGTCTTGGCACAGGTGGTACCAACTTCGACGCAAAGACCCGTCGTAACTCTACCGACCTCGAGGACATCTTCATCGCTCACATCTCCGGTATGGACGCTTGTGCACGCGCTCTCCTCAACGCAGTAGAGATACTCGAGAACTCTCCAATCCCAACAATGCTGAAGGACCGCTATGCAAGCTTCGACTCAGGTATGGGTAAGGACTTCGAGGACGGCAAGCTCACACTTGAGCAGGTTTATGAGTATGGTAAGAAGGTCGACGAGCCAAAGCAGACCTCTGGTAAGCAGGAACTCTATGAGACCATCGTTGCTCTCTATGCAAAATAA 5751MI3_001Neocallimastigales Amino 74MKEYFPQIGKIPFEGPESKNPMAFHYYDAERVVAGKTMKEWMRFAMAWWHTLCAEGSDQF AcidGPGTKKFPWNEGETALERAKHKADAGFEVMQKLGIPYFCFHDVDLIDEGANVAEYEANLAAITDYLKEKMEETGVKLLWSTANVFGNARYMNGASTNPDFDVVARAIVQIKNAIDAGIKLGAENYVFWGGREGYMSLLNTDQKREKEHMATMLGMARDYARAKGFTGTFLIEPKPMEPTKHQYDVDTETVIGFLKAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANRGDAQNGWDTDQFPIDNFELTQAWMQIVRNGGLGTGGTNFDAKTRRNSTDLEDIFIAHISGMDACARALLNAVEILENSPIPTMLKDRYASFDSGMGKDFEDGKLTLEQVYEYGKKVDEPKQTSGKQELYETIVALYAK 5753MI3_002 Prevotella DNA 75ATGCCTAAAGAATACTTCCCCTCCATCGGCAAAATCCCTTTTGAAGGAGGCGACAGCAAAAATCCCCTCGCTTTCCATTATTATGACGCCGGACGCGTGGTTATGGGCAAGCCCATGAAGGAATGGCTTAAATTCGCCATGGCCTGGTGGCACACGCTGGGCCAGGCCTCCGGAGACCCCTTCGGCGGCCAGACCCGCAGCTACGAATGGGACAAGGGCGAATGCCCCTACTGCCGCGCCAAAGCCAAGGCCGACGCCGGTTTTGAAATCATGCAAAAGCTGGGTATCGAATACTTCTGCTTCCACGATGTGGACCTTATCGAGGATTGCGATGACATTGCCGAATACGAAGCCCGCATGAAGGACATCACGGACTACCTGCTGGAAAAGATGAAGGAGACCGGCATCAAGAACCTCTGGGGCACCGCCAATGTCTTCGGCCACAAGCGCTACATGAACGGCGCCGGCACCAATCCGCAGTTCGATGTGGTGGCCCGTGCCGCCGTCCAGATCAAGAACGCCCTGGACGCCACCATCAAGCTGGGCGGCTCCAACTATGTGTTCTGGGGCGGCCGCGAAGGCTATTACACCCTCCTCAACACCCAGATGCAGCGGGAAAAAGACCACCTGGCCAAGTTGCTGACGGCCGCCCGCGACTATGCCCGCGCCAAGGGCTTCAAGGGCACCTTCCTCATTGAGCCCAAACCCATGGAACCCACCAAGCACCAGTACGACGTGGATACGGAGACGGTCATCGGCTTCCTCCGTGCCAACGGCCTGGACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCCACCCTGGCCGGCCACACCTTCGAGCATGAGCTCACCGTGGCCCGCGAGAACGGTTTCCTGGGCTCCATCGGTGCCAACCGCGGCGACGCCCAGAACGGCTGGGACACGGACCAGTTCCCTGTGGACCCGTACGATCTTACCCAGGCCATGATGCAGGTGCTGCTGAACGGCGGCTTCGGCAACGGCGGCACCAACTTCGACGCCAAACTCCGCCGCTCCTCCACCGACCCTGAGGACATCTTCATCGCCCATATTTCCGCCATGGATGCCATGGCCCACGCTTTGCTTAACGCAGCTGCCGTGCTGGAAGAGAGCCCCCTGTGCCAGATGGTCAAGGAGCGTTATGCCAGCTTCGACGGCGGCCTCGGCAAACAGTTCGAGGAAGGCAAGGCTACCCTGGAAGACCTGTACGAATACGCCAAGGTCCAGGGTGAACCCGTTGTCGCCTCCGGCAAGCAGGAGCTTTACGAGACTCTCCTGAACCTGTATGCCGTCAAGTAA 5753MI3_002Prevotella Amino 76MAKEYFPSIGKIPFEGGDSKNPLAFHYYDAGRVVMGKPMKEWLKFAMAWWHTLGQASGDP AcidFGGQTRSYEWDKGECPYCRAKAKADAGFEIMQKLGIEYFCFHDVDLIEDCDDIAEYEARMKDITDYLLEKMKETGIKNLWGTANVFGHKRYMNGAGTNPQFDVVARAAVQIKNALDATIKLGGSNYVFWGGREGYYTLLNTQMQREKDHLAKLLTAARDYARAKGFKGTFLIEPKPMEPTKHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIGANRGDAQNGWDTDQFPVDPYDLTQAMMQVLLNGGFGNGGTNFDAKLRRSSTDPEDIFIAHISAMDAMAHALLNAAAVLEESPLCQMVKERYASFDGGLGKQFEEGKATLEDLYEYAKVQGEPVVASGKQELYETLLNLYAVK 1754MI1_001 Prevotella DNA 77ATGGCAAAAGAGTATTTTCCGTTTACCGGTAAGATTCCTTTCGAAGGAAAGGACAGTAAGAATGTAATGGCTTTCCACTACTACGAGCCTGAGAAGGTCGTGATGGGAAAGAAGATGAAGGACTGGCTGAAGTTCGCTATGGCTTGGTGGCATACACTGGGTGGCGCTTCTGCTGACCAGTTTGGTGGTCAGACTCGTTCATACGAGTGGGACAAGGCTGGTGACGCTGTTCAGCGCGCTAAGGATAAGATGGACGCTGGCTTCGAGATCATGGACAAGCTGGGCATCGAGTACTTCTGCTTCCACGATGTTGACCTCGTTGAAGAGGGTGACACCATCGAGGAGTATGAGGCTCGCATGAAGGCCATCACCGACTACGCTCAGGAGAAGATGAAGCAGTTCCCCAACATCAAGCTGCTCTGGGGTACCGCAAACGTATTCGGTAACAAGCGCTATGCTAACGGTGCTTCTACCAACCCCGACTTCGACGTAGTGGCTCGCGCCATCGTTCAGATCAAGAACGCTATTGATGCTACCATCAAGCTGGGTGGTACCAACTATGTGTTCTGGGGTGGTCGTGAGGGCTATATGAGTCTGCTGAACACCGACCAGAAGCGTGAGAAGGAGCACATGGCTACTATGCTGACCATGGCTCGCGACTATGCTCGCGCCAAGGGATTCAAGGGTACATTCCTCATTGAGCCGAAGCCCATGGAGCCCAGCAAGCACCAGTATGATGTGGATACAGAGACCGTTATCGGCTTCCTGAAGGCACACAACCTGGACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCTACACTCGCTGGTCATACCTTCGAGCACGAGCTGGCTTGCGCTGTTGACGCTGGTATGCTTGGTTCTATCGACGCTAACCGTGGTGATGCTCAGAACGGTTGGGATACCGACCAGTTCCCCATCGACAACTACGAGCTGACACAGGCTATGCTCGAGATCATCCGCAATGGTGGTCTGGGCAATGGTGGTACCAACTTCGATGCTAAGATCCGTCGTAACAGCACCGACCTCGAGGATCTCTTCATCGCTCACATCAGTGGTATGGATGCTATGGCACGCGCTCTGATGAACGCTGCTGACATCCTTGAGAACTCTGAGCTGCCCGCAATGAAGAAGGCTCGCTACGCAAGCTTCGACCAGGGTGTTGGTAAGGACTTCGAAGATGGCAAGCTGACCCTTGAGCAGGTTTACGAGTATGGTAAGAAGGTGGGTGAGCCCAAGCAGACTTCTGGTAAGCAGGAGAAGTACGAGACCATCGTTGCTCTCTATGCAAAATAA 1754MI1_001Prevotella Amino 78MAKEYFPFTGKIPFEGKDSKNVMAFHYYEPEKVVMGKKMKDWLKFAMAWWHTLGGASADQ AcidFGGQTRSYEWDKAGDAVQRAKDKMDAGFEIMDKLGIEYFCFHDVDLVEEGDTIEEYEARMKAITDYAQEKMKQFPNIKLLWGTANVFGNKRYANGASTNPDFDVVARAIVQIKNAIDATIKLGGTNYVFWGGREGYMSLLNTDQKREKEHMATMLTMARDYARAKGFKGTFLIEPKPMEPSKHQYDVDTETVIGFLKAHNLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANRGDAQNGWDTDQFPIDNYELTQAMLEIIRNGGLGNGGTNFDAKIRRNSTDLEDLFIAHISGMDAMARALMNAADILENSELPAMKKARYASFDQGVGKDFEDGKLTLEQVYEYGKKVGEPKQTSGKQEKYETIVALYAK 1754MI3_007 Prevotella DNA 79ATGGCAAAAGAGTATTTTCCGTTTACCGGTAAGATTCCTTTCGAAGGAAAAGAGAGCAAGAACGTAATGGCTTTCCATTACTATGAGCCTGAAAAGGTGGTCATGGGCAAGAAAATGAAGGATTGGCTGAAATTCGCCATGGCTTGGTGGCACACCCTCGGTGGAGCCAGCGCCGACCAGTTCGGTGGACAGACCCGCAGCTATGAGTGGGACAAGGCCGAGGATGCCGTACAGCGTGCTAAGGACAAGATGGACGCCGGCTTCGAGATCATGGACAAACTGGGCATCGAGTATTTCTGCTTCCACGATGTCGACCTCGTCGACGAGGGTGCTACCGTTGAGGAGTATGAGGCTCGCATGAAAGCCATCACCGACTATGCCCAGGTCAAGATGAAGGAATATCCCAACATCAAACTGCTCTGGGGCACCGCCAACGTGTTCGGCAACAAGCGTTATGCCAACGGCGCTTCCACCAACCCCGACTTCGACGTGGTGGCACGCGCTATCGTTCAGATCAAGAATGCCATCGACGCTACCATCAAGCTCGGCGGTCAGAACTACGTGTTCTGGGGCGGACGCGAGGGCTACATGAGCCTGCTCAATACCGATCAGAAACGTGAGAAGGAACACATGGCCACCATGCTCACCATGGCGCGCGACTATGCTCGCAGCAAGGGATTCAAGGGCACCTTCCTCATCGAACCCAAACCCATGGAGCCTTCCAAGCACCAGTATGATGTCGACACCGAGACGGTCATCGGCTTCCTCCGCGCCCACAACCTCGACAAGGACTTCAAGGTGAACATCGAGGTCAACCACGCCACGCTCGCCGGCCACACCTTCGAGCACGAACTGGCTTGCGCCGTCGACGCCGGCATGCTCGGCAGCATCGACGCCAACCGCGGCGACGCACAGAACGGCTGGGATACCGACCAGTTCCCCATCGACAACTACGAACTGACACAGGCCATGCTGGAGATCATCCGCAATGGCGGCCTCGGCAATGGTGGTACCAACTTCGACGCCAAGATCCGTCGTAACAGCACCGACCTCGAAGATCTCTTCATCGCTCACATCAGCGGTATGGATGCCATGGCTCGCGCGCTGCTCAACGCCGCCGCCATCCTCGAGGAGAGCGAACTGCCCGCCATGAAGAAGGCCCGCTACGCTTCCTTCGACGAAGGTATCGGCAAGGACTTCGAAGACGGCAAACTCACCCTCGAGCAGGTTTACGAGTACGGCAAGAAGGTAGGCGAGCCCAAGCAGACCTCCGGCAAGCAAGAGAAGTACGAGACCATCGTGGCTCTCTACAGCAAATAA 1754MI3_007Prevotella Amino 80MAKEYFPFTGKIPFEGKESKNVMAFHYYEPEKVVMGKKMKDWLKFAMAWWHTLGGASADQ AcidFGGQTRSYEWDKAEDAVQRAKDKMDAGFEIMDKLGIEYFCFHDVDLVDEGATVEEYEARMKAITDYAQVKMKEYPNIKLLWGTANVFGNKRYANGASTNPDFDVVARAIVQIKNAIDATIKLGGQNYVFWGGREGYMSLLNTDQKREKEHMATMLTMARDYARSKGFKGTFLIEPKPMEPSKHQYDVDTETVIGFLRAHNLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANRGDAQNGWDTDQFPIDNYELTQAMLEIIRNGGLGNGGTNFDAKIRRNSTDLEDLFIAHISGMDAMARALLNAAAILEESELPAMKKARYASFDEGIGKDFEDGKLTLEQVYEYGKKVGEPKQTSGKQEKYETIVALYSK 1754MI5_009 Prevotella DNA 81ATGAAAGAGTATTTCCCGCAAATTGGAAAGATTCCCTTCGAGGGACCAGAGAGCAAGAGTCCATTGGCGTTCCATTATTATGAGCCGGATCGCATGGTGCTCGGAAAGAGGATGGAGGATTGGCTGAAATTCGCCATGGCATGGTGGCACACCCTTGGCCAGGCCAGCGGCGACCAGTTCGGCGGACAGACACGTGAGTACGAGTGGGATAAGGCTGGAGATCCGATACAAAGGGCAAAGGATAAGATGGACGCCGGATTCGAGATCATGGAGAAATTGGGTATCAAGTACTTCTGCTTCCATGATGTGGATCTCGTCGAGGAAGCTCCCACCATCGCCGAATATGAGGAGCGTATGAGGATCATCACCGACTATGCGCTCGAGAAGATGAAAGCCACTGGCATCAAACTCCTTTGGGGTACAGCCAATGTTTTCGGACATAAGAGATATATGAATGGGGCCGCCACCAACCCGGAGTTCGGTGTTGTCGCCAGGGCTGCTGTCCAGATCAAGAACGCGATCGACGCCACCATCAAGCTGGGAGGAACAAACTATGTGTTCTGGGGTGGCCGCGAGGGCTACATGAGCCTGCTCAACACCCAGATGCAGAGGGAGAAGGACCATCTCGCCAATATGCTCAAGGCTGCTCGTGACTATGCTCGCGCCAAGGGATTCAAGGGCACATTCCTCATCGAGCCGAAGCCGATGGAACCTACTAAGCATCAGTACGATGTCGACACTGAGACCGTGATCGGCTTCCTCCGCGCAAACGGTCTTGACAAGGATTTCAAGGTCAACATCGAGGTCAATCACGCCACTCTTGCGGGTCACACTTTCGAGCATGAGCTCGCCGTGGCTGTCGACAATGGTCTCCTTGGCTCAATCGATGCGAACAGGGGAGATTATCAGAACGGTTGGGACACCGACCAGTTCCCTGTTGATCTCTTTGATTTGACCCAGGCCATGCTCCAGATCATCCGTAACGGAGGCCTCGGTAATGGTGGATCCAACTTCGACGCCAAGCTTCGCCGTAACTCCACTGATCCTGAGGATATATTCATTGCCCATATTTGCGGTATGGACGCTATGGCCAGGGCTCTCCTTGCCGCCGCCGCGATCGTGGAGGAGTCTCCTATCCCGGCTATGGTCAAAGAGCGTTACGCATCCTTCGACGAAGGTGAGGGCAAGAGATTCGAGGATGGTAAGATGAGTCTGGAGGAACTTGTTGATTACGCGAAGACTCACGGAGAGCCCGCCCAGAAGAGTGGCAAACAGGAGCTCTACGAAACCCTTGTCAACATGTACATCAAATAA 1754MI5_009Prevotella Amino 82MKEYFPQIGKIPFEGPESKSPLAFHYYEPDRMVLGKRMEDWLKFAMAWWHTLGQASGDQF AcidGGQTREYEWDKAGDPIQRAKDKMDAGFEIMEKLGIKYFCFHDVDLVEEAPTIAEYEERMRIITDYALEKMKATGIKLLWGTANVFGHKRYMNGAATNPEFGVVARAAVQIKNAIDATIKLGGTNYVFWGGREGYMSLLNTQMQREKDHLANMLKAARDYARAKGFKGTFLIEPKPMEPTKHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELAVAVDNGLLGSIDANRGDYQNGWDTDQFPVDLFDLTQAMLQIIRNGGLGNGGSNFDAKLRRNSTDPEDIFIAHICGMDAMARALLAAAAIVEESPIPAMVKERYASFDEGEGKRFEDGKMSLEELVDYAKTHGEPAQKSGKQELYETLVNMYIK 5586MI1_003 Prevotella DNA 83ATGGCAAAAGAGTATTTTCCGTTTACCGGTAAGATTCCTTTCGAGGGAAAGGACAGTAAGAATGTAATGGCGTTCCACTACTACGAGCCCGAGCGCGTGGTAATGGGCAAGAAGATGAAGGAGTGGCTGAAGTTTGCCATGGCCTGGTGGCACACGCTGGGTGGAGCCAGTGCCGACCAGTTTGGCGGACAGACCCGCAGCTACGAGTGGGACAAGGCTGAAGACGCCGTGCAGCGTGCCAAGGACAAGATGGATGCCGGCTTCGAGATCATGGACAAGCTGGGCATCGAGTATTTCTGCTTCCATGATGTCGATCTCGTTGACGAGGGTGCCACTGTCGAGGAGTATGAGGCTCGCATGCAGGCCATCACCGACTATGCGCAGGAGAAGATGAAGCAGTATCCTGCCATCAAGCTGCTGTGGGGTACGGCCAATGTCTTTGGCAACAAGCGTTATGCCAACGGTGCCTCTACCAATCCCGACTTCGATGTGGTGGCCCGCGCCATCGTGCAGATTAAGAATGCCATTGATGCCACCATCAAGCTGGGCGGCAGCAACTATGTGTTCTGGGGCGGTCGCGAGGGCTACATGTCGCTGCTCAACACCGACCAGAAGCGTGAGAAGGAACACATGGCCCGGATGCTGACCATGGCCCGCGACTATGCCCGCTCGAAGGGCTTCAAGGGCAACTTCCTGATTGAGCCCAAGCCCATGGAGCCGTCGAAGCATCAGTACGACGTGGACACCGAGACGGTTATCGGATTCCTCCGCGCACATGGCCTTGACAAGGACTTCAAGGTGAACATCGAGGTGAACCATGCCACGCTGGCCGGTCATACCTTCGAGCACGAACTGGCTTGCGCCGTAGATGCCGGCATGCTGGGCAGCATTGATGCCAACCGCGGCGACGCACAGAACGGATGGGACACCGACCAGTTCCCCATCGACAACTATGAGTTGACACAGGCCATGATGGAGATTATCCGCAATGGCGGTCTGGGTCTTGGCGGTACCAATTTCGATGCCAAGATTCGCCGTAACTCCACCGACCTGGAAGACCTCTTCATCGCCCACATCAGTGGCATGGACGCCATGGCTCGTGCGCTCCTTAATGCTGCCGACATTCTGGAGAACAGCGAACTGCCCGCCATGAAGAAAGCGCGCTACGCCTCGTTCGACAGTGGCATGGGCAAGGACTTCGAGGACGGCAAACTGACCCTTGAGCAGGTTTACGAATACGGCAAAAAAGTCGGCGAACCTAAGCAGACCTCCGGCAAGCAGGAGAAGTACGAGACCATCGTGGCTCTCTATGCCAAGTAA 5586MI1_003Prevotella Amino 84MAKEYFPFTGKIPFEGKDSKNVMAFHYYEPERVVMGKKMKEWLKFAMAWWHTLGGASADQ AcidFGGQTRSYEWDKAEDAVQRAKDKMDAGFEIMDKLGIEYFCFHDVDLVDEGATVEEYEARMQAITDYAQEKMKQYPAIKLLWGTANVFGNKRYANGASTNPDFDVVARAIVQIKNAIDATIKLGGSNYVFWGGREGYMSLLNTDQKREKEHMARMLTMARDYARSKGFKGNFLIEPKPMEPSKHQYDVDTETVIGFLRAHGLDKDEKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANRGDAQNGWDTDQFPIDNYELTQAMMEIIRNGGLGLGGTNFDAKIRRNSTDLEDLFIAHISGMDAMARALLNAADILENSELPAMKKARYASFDSGMGKDFEDGKLTLEQVYEYGKKVGEPKQTSGKQEKYETIVALYAK 5586MI2_006 Prevotella DNA 85ATGGCAAAAGAGTATTTTCCGTTTACAGGTAAAATTCCTTTCGAAGGAAAGGACAGTAAGAACGTAATGGCTTTCCACTACTACGAGCCCGAAAAGGTCGTGATGGGAAAGAAAATGAAAGACTGGCTGAAGTTCGCCATGGCCTGGTGGCACACACTGGGTGGCGCCAGCGCCGACCAGTTTGGCGGCCAGACACGCAGCTATGAGTGGGACAAGGCTGCCGATGCCGTGCAGCGCGCAAAGGACAAGATGGACGCCGGCTTCGAAATCATGGACAAGCTGGGCATCGAGTATTTCTGCTTCCACGACGTGGACCTCGTTGAGGAGGGAGCCACCATCGAGGAGTATGAGGCCCGCATGAAGGCTATCACCGACTATGCCCAGGAGAAGATGAAACAGTATCCCAGCATCAAGCTGCTCTGGGGCACCGCCAATGTGTTTGGCAACAAGCGCTACGCCAACGGCGCCAGCACCAACCCCGACTTCGACGTCGTGGCCCGTGCCATCGTGCAGATCAAGAACGCCATCGATGCCACCATCAAGCTGGGCGGCACCAACTACGTGTTCTGGGGCGGACGCGAGGGCTACATGAGCCTGCTCAACACCGACCAGAAGCGCGAGAAGGAGCACATGGCCACCATGCTCACCATGGCCCGCGACTACGCCCGCGCAAAGGGATTCAAGGGCACCTTCCTCATCGAGCCCAAGCCCATGGAGCCGTCGAAGCACCAGTACGACGTGGACACCGAGACCGTCATCGGTTTCCTGAAGGCCCACGGTCTGGACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCCACGCTGGCCGGCCACACCTTCGAGCATGAGCTGGCCTGCGCCGTCGACGCCGGTATGCTGGGCAGCATCGATGCCAACCGCGGCGACGCCCAGAACGGCTGGGACACCGACCAGTTCCCCATCGACAACTTCGAGCTCACCCAGGCCATGATGGAAATTATCCGCAACGGCGGCCTCGGCAACGGCGGCACCAACTTCGACGCTAAGATCCGCCGCAACTCCACCGACCTCGAGGACCTCTTCATCGCCCACATCAGCGGCATGGACGCCATGGCCCGCGCACTGATGAACGCTGCCGACATTATGGAGAACAGCGAGCTGCCCGCCATGAAGAAGGCACGCTACGCCAGCTTCGACGCCGGCATCGGCAAGGACTTTGAGGATGGCAAGCTCTCGCTGGAGCAGGTCTACGAGTATGGCAAGAAGGTGGAAGAGCCCAAGCAGACCAGCGGCAAGCAGGAGAAGTACGAGACCATCGTCGCCCTCTATGCCAAGTAA 5586MI2_006Prevotella Amino 86MAKEYFPFTGKIPFEGKDSKNVMAFHYYEPEKVVMGKKMKDWLKFAMAWWHTLGGASADQ AcidFGGQTRSYEWDKAADAVQRAKDKMDAGFEIMDKLGIEYFCFHDVDLVEEGATIEEYEARMKAITDYAQEKMKQYPSIKLLWGTANVFGNKRYANGASTNPDFDVVARAIVQIKNAIDATIKLGGTNYVFWGGREGYMSLLNTDQKREKEHMATMLTMARDYARAKGFKGTFLIEPKPMEPSKHQYDVDTETVIGFLKAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANRGDAQNGWDTDQFPIDNFELTQAMMEIIRNGGLGNGGTNFDAKIRRNSTDLEDLFIAHISGMDAMARALMNAADIMENSELPAMKKARYASFDAGIGKDFEDGKLSLEQVYEYGKKVEEPKQTSGKQEKYETIVALYAK 5586MI8_003 Prevotella DNA 87ATGGCAAAAGAGTATTTCGCCTTTACAGGCAAGATTCCTTTCGAGGGAAAAGACAGTAAGAACGTGATGGCTTTCCACTACTACGAGCCGGAGCGTGTGGTGATGGGCAAGAAGATGAAGGAGTGGCTGAAGTTCGCCATGGCCTGGTGGCACACACTGGGTGGCGCATCGGCCGACCAGTTCGGAGGCCAGACACGCAGCTACGAGTGGGACAAGGCCGCCGACGCCGTGCAGCGCGCCAAGGACAAGATGGACGCCGGCTTCGAGATTATGGACAAGCTGGGCATCGAGTACTTCTGCTTCCACGATGTAGACCTCGTTGAGGAGGGTGAGACCATAGCCGAGTACGAGCGCCGCATGAAGGAAATCACCGACTACGCACAGGAGAAGATGAAGCAGTTCCCCAACATCAAGCTGCTCTGGGGCACAGCCAACGTGTTCGGCAACAAGCGCTACGCCAACGGCGCATCGACCAACCCCGACTTCGACGTTGTGGCACGCGCCATCGTGCAGATCAAGAACGCCATCGACGCCACCATCAAGCTCGGCGGCTCCAACTATGTGTTCTGGGGCGGACGCGAGGGCTATATGAGCCTGCTCAACACCGACCAGAAGCGCGAGAAGGAGCACATGGCCACCATGCTCACCATGGCCCGCGACTATGCACGCGCCAAGGGATTCAAGGGCACATTCCTCATCGAGCCGAAGCCCATGGAGCCCTCGAAGCACCAGTACGACGTAGACACAGAGACCGTCATCGGCTTCCTCCGTGCACACGGGCTGGACAAGGACTTCAAGGTGAACATCGAGGTAAACCACGCCACACTGGCCGGCCACACCTTCGAGCACGAGCTGGCTTGCGCCGTCGACGCTGGCATGCTGGGCAGCATCGACGCCAACCGTGGCGACGCACAGAACGGATGGGACACCGACCAGTTCCCCATCGACAACTTCGAGCTCACACAGGCCATGATGGAAATCATCCGCAATGGCGGACTGGGCAATGGCGGCACCAACTTCGACGCCAAGATCCGTCGTAACAGCACCGACCTCGAAGACCTCTTCATCGCCCACATCAGCGGCATGGACGCCATGGCACGCGCACTGCTCAACGCTGCCGACATCCTGGAGCACAGCGAGCTGCCCAAGATGAAGAAGGAGCGCTACGCCAGCTTCGACGCAGGCATCGGCAAGGACTTCGAAGACGGCAAGCTCACACTCGAGCAGGTCTACGAGTACGGCAAGAAGGTCGAAGAGCCCCGTCAGACCAGCGGCAAGCAGGAGAAGTACGAGACCATCGTCGCCCTCTATGCCAAGTAA 5586MI8_003Prevotella Amino 88MAKEYFAFTGKIPFEGKDSKNVMAFHYYEPERVVMGKKMKEWLKFAMAWWHTLGGASADQ AcidFGGQTRSYEWDKAADAVQRAKDKMDAGFEIMDKLGIEYFCFHDVDLVEEGETIAEYERRMKEITDYAQEKMKQFPNIKLLWGTANVFGNKRYANGASTNPDFDVVARAIVQIKNAIDATIKLGGSNYVFWGGREGYMSLLNTDQKREKEHMATMLTMARDYARAKGFKGTFLIEPKPMEPSKHQYDVDTETVIGFLRAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANRGDAQNGWDTDQFPIDNFELTQAMMEIIRNGGLGNGGTNFDAKIRRNSTDLEDLFIAHISGMDAMARALLNAADILEHSELPKMKKERYASFDAGIGKDFEDGKLTLEQVYEYGKKVEEPRQTSGKQEKYETIVALYAK 5586MI14_003 Prevotella DNA 89ATGGCAAAAGAGTATTTTCCGTTTACTGGTAAGATTCCTTTCGAGGGAAAGGATAGTAAGAATGTAATGGCTTTCCACTATTACGAGCCCGAGAAAGTCGTGATGGGAAAGAAGATGAAGGACTGGCTGAAGTTCGCAATGGCTTGGTGGCATACACTGGGTGGTGCATCTGCAGACCAGTTCGGTGGAGAGACCCGCAGCTACGAGTGGAGCAAGGCTGCTGATCCCGTTCAGCGCGCCAAGGACAAGATGGACGCCGGCTTTGAGATTATGGATAAGCTGGGCATCGAGTACTTCTGTTTCCACGATATAGACCTCGTTCAGGAGGCAGATACCATTGCAGAATATGAGGAGCGCATGAAGGCAATTACCGACTATGCTCTGGAGAAGATGAAGCAGTTCCCCAACATCAAGTTGCTCTGGGGTACCGCTAACGTATTTAGCAACAAGCGCTATATGAACGGTGCTTCTACCAATCCCGACTTCGACGTGGTGGCCCGTGCCATCGTTCAGATCAAGAACGCTATTGATGCAACCATCAAACTCGGTGGTACCAACTATGTATTCTGGGGTGGTCGTGAGGGTTACATGAGCCTATTGAATACCGACCAGAAGCGTGAAAAGGAGCACATGGCAATGATGCTCGGTATGGCTCGCGACTATGCCCGCAGCAAGGGATTCAAGGGTACGTTCCTCATCGAGCCGAAGCCGATGGAGCCCTCTAAGCATCAGTATGATGTCGATACGGAGACTGTGATTGGTTTCCTGAAGGCACACGGTCTGGACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCTACACTGGCTGGTCATACCTTCGAGCATGAGCTGGCTTGCGCTGTTGACGCAGGTATGCTGGGCTCTATCGACGCTAACCGCGGTGATGCCCAGAACGGCTGGGATACCGACCAGTTCCCCATCGACAACTACGAGCTGACACAGGCTATGATGGAAATCATCCGCAACGGTGGTCTGGGCAATGGTGGTACCAACTTCGACGCTAAGATCCGCCGTAACTCTACCGACCTCGAGGATCTGTTCATCGCTCATATCAGTGGTATGGATGCTATGGCCCGTGCTTTGTTGAATGCTGCCGACATTCTGGAGAACTCTGAACTGCCCGCTATGAAGAAGGCCCGCTACGCCAGCTTCGACAACGGTATCGGTAAGGACTTCGAGGATGGCAAGCTGACCTTCGAGCAGGTTTACGAATATGGTAAGAAAGTTGAAGAGCCGAAGCAGACCTCTGGCAAGCAGGAGAAATACGAGACCATCGTTGCTCTGTATGCTAAATAA5586MI14_003 Prevotella Amino 90MAKEYFPFTGKIPFEGKDSKNVMAFHYYEPEKVVMGKKMKDWLKFAMAWWHTLGGASADQ AcidFGGETRSYEWSKAADPVQRAKDKMDAGFEIMDKLGIEYFCFHDIDLVQEADTIAEYEERMKAITDYALEKMKQFPNIKLLWGTANVFSNKRYMNGASTNPDFDVVARAIVQIKNAIDATIKLGGTNYVFWGGREGYMSLLNTDQKREKEHMAMMLGMARDYARSKGFKGTFLIEPKPMEPSKHQYDVDTETVIGFLKAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANRGDAQNGWDTDQFPIDNYELTQAMMEIIRNGGLGNGGTNFDAKIRRNSTDLEDLFIAHISGMDAMARALLNAADILENSELPAMKKARYASFDNGIGKDFEDGKLTFEQVYEYGKKVEEPKQTSGKQEKYETIVALYAK 5586MI26_003 Prevotella DNA 91ATGGCAAAAGAGTATTTTCCGTTTACCGGTAAAATTCCTTTCGAGGGAAAGGACAGTAAGAATGTAATGGCTTTCCACTACTACGAGCCTGAGCGCGTAGTGATGGGAAAGAAGATGAAGGATTGGTTGCGATTTGCAATGGCTTGGTGGCACACACTGGGTGGCGCTTCTGCCGACCAGTTTGGTGGTCAGACCCGCAGTTACGAATGGGACAAGGCTGCTGATGCTGTTCAGCGTGCTAAGGACAAGATGGATGCCGGCTTCGAGATTATGGATAAGCTGGGAATCGAGTTCTTCTGCTGGCACGATATCGACCTCGTTGAAGAGGGTGAGACCATTGAAGAGTATGAGCGCCGCATGAAGGCTATCACCGACTATGCTCTTGAGAAGATGCAGCAGTATCCCAACATCAAGAACCTCTGGGGAACAGCCAATGTGTTTGGCAACAAGCGTTATGCCAACGGTGCCAGCACAAACCCAGACTTTGACGTCGTTGCTCGTGCTATCGTACAGATTAAGAATGCTATCGACGCTACTATCAAGTTGGGTGGTCAGAATTATGTGTTCTGGGGTGGCCGTGAGGGCTACATGAGCCTGCTCAATACTGACCAGAAGCGTGAGAAGGAGCACATGGCTACAATGCTGACCATGGCACGCGACTATGCCCGCAGCAAGGGATTCAAGGGTAACTTCCTCATTGAGCCCAAGCCCATGGAGCCGTCAAAGCACCAGTATGATGTTGACACCGAGACCGTATGCGGTTTCCTGCGTGCCCACAACCTTGACAAGGATTTCAAGGTAAATATCGAGGTTAACCATGCTACTCTGGCTGGTCATACTTTCGAGCACGAACTGGCATGCGCTGTTGACGCTGGTATGCTTGGTTCTATCGATGCTAACCGTGGTGATGCCCAGAATGGCTGGGATACCGACCAGTTCCCCATCAACAACTATGAACTCACTCAGGCTATGCTTGAGATCATCCGTAATGGTGGTCTGGGTCTTGGCGGCACAAACTTCGATGCCAAGATTCGTCGTAACTCAACAGATCTTGAGGATCTCTTCATCGCTCACATCAGTGGTATGGATGCCATGGCCCGTGCTCTGCTGAATGCTGCTGCTATTCTGGAGGAGAGCGAGCTGCCTAAGATGAAGAAGGAGCGTTATGCTTCTTTCGATGCCGGTATCGGTAAGGACTTCGAGGATGGCAAGCTTACCCTTGAGCAGGCTTACGAGTATGGTAAGAAGGTTGAGGAGCCCAAGCAGACTTCAGGCAAGCAGGAGAAGTACGAGACCATCGTTGCTCTGTATGCAAAATAA5586MI26_003 Prevotella Amino 92MAKEYFPFTGKIPFEGKDSKNVMAFHYYEPERVVMGKKMKDWLRFAMAWWHTLGGASADQ AcidFGGQTRSYEWDKAADAVQRAKDKMDAGFEIMDKLGIEFFCWHDIDLVEEGETIEEYERRMKAITDYALEKMQQYPNIKNLWGTANVFGNKRYANGASTNPDFDVVARAIVQIKNAIDATIKLGGQNYVFWGGREGYMSLLNTDQKREKEHMATMLTMARDYARSKGEKGNFLIEPKPMEPSKHQYDVDTETVCGFLRAHNLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANRGDAQNGWDTDQFPINNYELTQAMLEIIRNGGLGLGGTNFDAKIRRNSTDLEDLFIAHISGMDAMARALLNAAAILEESELPKMKKERYASFDAGIGKDFEDGKLTLEQAYEYGKKVEEPKQTSGKQEKYETIVALYAK 5586MI86_001 Prevotella DNA 93ATGAAACAGTATTTTCCCCAGATTGGAAAGATACCCTTCGAGGGTGTAGAGAGCAAGAATGTGATGGCTTTCCACTATTATGAGCCAGAAAGAGTAGTCATGGGCAAGCCTATGAAAGAATGGCTGCGCTTCGCTATGGCGTGGTGGCACACGCTGGGGCAGGCGAGCGGCGACCCCTTCGGCGGACAGACCCGCAGCTACGAGTGGGACCGTGCGGCCGACGCGCTACAGCGCGCCAAGGACAAGATGGATGCGGGCTTCGAGCTGATGGAGAAGCTTGGCATTGAGTACTTCTGCTTCCACGACGTGGACCTCGTAGAAGAGGGCGCCACGGTGGAGGAATACGAGCGGCGGATGGCTGCCATCACCGACTACGCGGTAGAGAAGATGCGCGAGCATCCCGAGATACACTGCCTGTGGGGCACGGCCAATGTCTTCGGCCACAAGCGCTACATGAACGGAGCCGCCACCAACCCCGACTTCGACGTGGTGGCGCGTGCGGTGGTGCAGATAAAGAACAGCATCGACGCCACGATCAAGCTGGGCGGCGAGAACTATGTGTTCTGGGGCGGACGCGAGGGATATATGAGCCTGCTCAACACCGACCAGCGCCGCGAGAAGGAGCACCTGGCCATGATGCTTGCGAAGGCCCGCGACTATGGCCGCGCCCACGGCTTCAAGGGCACCTTCCTGATAGAGCCCAAGCCGATGGAGCCCATGAAGCACCAGTACGACGTGGACACCGAGACGGTGATAGGTTTCCTGCGTGCCCACGGACTGGACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCCACGTTGGCGGGCCACACGTTCGAGCACGAGCTGGCCTGTGCCGTCGATGCCGGCATGCTGGGCAGCATCGACGCCAACCGTGGCGACGCGCAGAACGGATGGGATACGGACCAGTTCCCCATAGACTGCTACGAGCTCACGCAGGCGTGGATGGAGATCATTCGTGGCGGCGGCTTCACCACCGGCGGCACCAACTTCGACGCTAAGCTGCGCCGCAACTCGACCGACCCCGAGGATATCTTCATAGCTCACATCAGCGGCATGGATGCTATGGCCCGCGCCCTGCTCTGCGCCGCCGACATCTTGGAGCACAGCGAGCTGCCGGAGATGAAGCGGAAGCGCTATGCCTCGTTCGACAGCGGCATGGGCAAGGAGTTCGAAGAGGGCAATCTCAGCTTCGAGCAAATCTATGCCTACGGCAAGCAGGCGGGCGAACCGGCCACGACCAGCGGCAAGCAGGAGAAATACGAAGCCATTGTTTCACTTTATACCCGATGA 5586MI86_001Prevotella Amino 94MKQYFPQIGKIPFEGVESKNVMAFHYYEPERVVMGKPMKEWLRFAMAWWHTLGQASGDPF AcidGGQTRSYEWDRAADALQRAKDKMDAGFELMEKLGIEYFCFHDVDLVEEGATVEEYERRMAAITDYAVEKMREHPEIHCLWGTANVFGHKRYMNGAATNPDFDVVARAVVQIKNSIDATIKLGGENYVFWGGREGYMSLLNTDQRREKEHLAMMLAKARDYGRAHGFKGTFLIEPKPMEPMKHQYDVDTETVIGFLRAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANRGDAQNGWDTDQFPIDCYELTQAWMEIIRGGGFTTGGTNFDAKLRRNSTDPEDIFIAHISGMDAMARALLCAADILEHSELPEMKRKRYASFDSGMGKEFEEGNLSFEQIYAYGKQAGEPATTSGKQEKYEAIVSLYTR 5586MI108_002 Prevotella DNA 95ATGGCAAAAGAGTATTTTCCGTTTATCGGTAAGGTTCCTTTCGAAGGAACAGAGAGCAAGAACGTGATGGCATTCCACTACTATGAGCCCGAAAAGGTGGTCATGGGTAAGAAAATGAAGGACTGGCTGAAGTTCGCTATGGCTTGGTGGCACACACTGGGTGGTGCCAGCGCCGACCAGTTTGGTGGTCAGACTCGCAGCTACGAGTGGGACAAGGCTGCTGATGCCGTTCAGCGCGCCAAGGACAAGATGGATGCTGGCTTCGAGATCATGGATAAGCTCGGCATTGAGTACTTCTGCTTCCATGACGTAGACCTCGTTGAGGAGGGTGAAACCGTCGCTGAGTATGAGGCTCGCATGAAGGTCATCACCGACTATGCCCTGGAGAAGATGCAGCAGTTCCCCAACATCAAACTGCTCTGGGGTACTGCTAACGTGTTCGGCCACAAGCGCTATGCCAACGGTGCCAGCACCAATCCCGACTTCGACGTCGTGGCCCGTGCTATCGTTCAGATCAAGAATGCCATCGATGCTACCATTAAGCTCGGCGGTACGAACTATGTGTTCTGGGGTGGTCGTGAGGGCTACATGAGCCTTCTCAACACCGACCAGAAGCGCGAGAAGGAGCACATGGCAACGATGCTGACCATGGCTCGCGACTATGCCCGCGCCAAGGGATTCAAGGGCACGTTCCTCATCGAGCCGAAGCCCATGGAGCCCTCGAAGCATCAGTACGACGTCGACACCGAGACCGTCATCGGCTTCCTCCGTGCCCACGGTCTGGATAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCCACGCTGGCCGGTCATACCTTCGAGCACGAACTGGCTTGCGCCGTTGATGCCGGCATGCTCGGCTCTATCGATGCCAACCGCGGCGACGCTCAGAACGGCTGGGACACCGACCAGTTCCCCATCGACAACTACGAGCTCACTCAGGCCATGATGGAAATCATCCGTAATGGCGGTCTGGGCAACGGCGGCACGAACTTCGATGCCAAGATCCGTCGTAACAGCACCGACCTCGAGGACCTCTTCATCGCTCACATCAGCGGCATGGATGCCATGGCACGCGCTCTGATGAACGCTGCTGCCATCCTCGAAGAGAGCGAGCTGCCCGCCATGAAGAAGGCCCGCTATGCTTCGTTCGACGAGGGTATCGGCAAGGACTTCGAGGACGGCAAGTTGTCACTTGAGCAGGTCTACGAATATGGTAAGAAGGTTGAGGAGCCCAAGCAGACCTCGGGCAAGCAGGAGAAGTACGAGACCATCGTGGCCCTCTATGCCAAGTAA5586MI108_002 Prevotella Amino 96MAKEYFPFIGKVPFEGTESKNVMAFHYYEPEKVVMGKKMKDWLKFAMAWWHTLGGASADQ AcidFGGQTRSYEWDKAADAVQRAKDKMDAGFEIMDKLGIEYFCFHDVDLVEEGETVAEYEARMKVITDYALEKMQQFPNIKLLWGTANVFGHKRYANGASTNPDFDVVARAIVQIKNAIDATIKLGGTNYVFWGGREGYMSLLNTDQKREKEHMATMLTMARDYARAKGFKGTFLIEPKPMEPSKHQYDVDTETVIGFLRAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDAGMLGSIDANRGDAQNGWDTDQFPIDNYELTQAMMEIIRNGGLGNGGTNFDAKIRRNSTDLEDLFIAHISGMDAMARALMNAAAILEESELPAMKKARYASFDEGIGKDFEDGKLSLEQVYEYGKKVEEPKQTSGKQEKYETIVALYAK 5586MI182_004 Prevotella DNA 97ATGGCAAAAGAGTATTTTCCGTTTGTTGGTAAGATTCCTTTCGAGGGAAAGGATAGTAAGAATGTAATGGCTTTCCACTATTACGAACCAGAGAAGGTCGTGATGGGAAAGAAGATGAAGGACTGGCTGAAGTTCGCCATGGCATGGTGGCACACACTGGGACAGGCCAGTGCCGACCCGTTTGGAGGTCAGACCCGCAGCTACGAGTGGGACAAGGCTGACGATGCTGTGCAGCGCGCAAAGGACAAGATGGATGCCGGATTTGAGATCATGGACAAGCTGGGCATCGAGTACTTCTGCTTCCACGATGTAGACCTCGTTGAGGAGGGAGCAACTGTTGAGGAGTACGAGGCTCGCATGAAGGCCATCACCGACTATGCATTGGAGAAGATGAAAGAGTATCCCAACATCAAGAACCTCTGGGGTACAGCCAATGTATTCAGCAACAAGCGCTATATGAACGGTGCCAGCACCAACCCCGACTTCGACGTTGTTGCACGTGCCATCGTACAGATAAAGAACGCCATTGACGCTACCATCAAGCTCGGCGGTCAGAACTACGTGTTCTGGGGCGGACGTGAGGGATACATGAGCCTGCTCAACACCGACCAGAAGCGCGAGAAGGAGCACATGGCAACCATGCTGACCATGGCTCGCGACTACGCTCGCAAGAACGGTTTCAAGGGCACATTCCTCATCGAGCCTAAGCCCATGGAACCCTCAAAGCACCAGTACGACGTAGACACAGAGACCGTATGCGGTTTCCTCCGCGCCCATGGTCTTGACAAGGATTTCAAGGTGAACATTGAGGTGAACCACGCTACCCTCGCCGGCCACACCTTTGAGCATGAACTGGCTTGCGCCGTCGACAACGGCATGCTCGGCAGCATCGATGCCAACCGCGGCGACGTTCAGAACGGCTGGGACACCGACCAGTTCCCCATCGACAACTACGAGCTGACTCAGGCCATGCTCGAAATCATCCGCAACGGTGGTCTGGGCAACGGCGGTACCAACTTCGACGCCAAGATCCGTCGTAACTCTACCGACCTCGAGGATCTGTTCATCGCCCACATCAGCGGTATGGACGCCATGGCACGTGCACTGCTCAATGCAGCAGCCATACTGGAGGAGAGCGAGCTGCCTGCCATGAAGAAGGAGCGTTACGCCAGCTTCGACAGCGGCATCGGCAAGGACTTCGAGGACGGCAAGCTCACACTTGAGCAGGCCTATGAGTATGGTAAGAAGGTTGAGGAGCCAAAGCAGACCTCTGGCAAGCAGGAGAAGTATGAGACTATAGTAGCCCTCTACGCTAAGTAG5586MI182_004 Prevotella Amino 98MAKEYFPFVGKIPFEGKDSKNVMAFHYYEPEKVVMGKKMKDWLKFAMAWWHTLGQASADP AcidFGGQTRSYEWDKADDAVQRAKDKMDAGFEIMDKLGIEYFCFHDVDLVEEGATVEEYEARMKAITDYALEKMKEYPNIKNLWGTANVFSNKRYMNGASTNPDFDVVARAIVQIKNAIDATIKLGGQNYVFWGGREGYMSLLNTDQKREKEHMATMLTMARDYARKNGFKGTFLIEPKPMEPSKHQYDVDTETVCGFLRAHGLDKDFKVNIEVNHATLAGHTFEHELACAVDNGMLGSIDANRGDVQNGWDTDQFPIDNYELTQAMLEIIRNGGLGNGGTNFDAKIRRNSTDLEDLFIAHISGMDAMARALLNAAAILEESELPAMKKERYASFDSGIGKDFEDGKLTLEQAYEYGKKVEEPKQTSGKQEKYETIVALYAK 5586MI193_004 Prevotella DNA 99ATGACTAAAGAGTATTTCCCTACCATTGGCAAGATTCCCTTTGAGGGACCTGAAAGCAAGAACCCGCTTGCATTCCATTACTATGAGCCCGACCGCCTGGTCATGGGCAAGAAGATGAAAGACTGGCTGCGTTTCGCCATGGCCTGGTGGCACACCCTGGGCCAGGCCTCCGGCGACCAGTTCGGCGGCCAGACCCGCCACTATGCCTGGGATGATCCGGATTGCCCGTATGCACGTGCCAAAGCCAAGGCCGACGCCGGTTTCGAAATCATGCAGAAACTGGGCATTGAATTCTTCTGCTTCCACGACATCGACCTGGTCGAGGATGCCGATGAAATCGCCGAGTACGAGGCCCGGATGAAGGACATCACCGACTATCTGCTCGTCAAGATGAAAGAGACCGGCATCAAGAACCTTTGGGGAACGGCCAACGTATTTGGCCACAAGCGCTACATGAACGGCGCCGCCACCAACCCCGATTTCGACGTGCTGGCCCGTGCCGCCGTCCAGATCAAGAACGCCATCGACGCCACCATCAAGTTGGGCGGTCAGAACTATGTGTTCTGGGGCGGCCGTGAAGGCTACCAGACCCTGCTCAATACCCAGATGCAGCGCGAGAAGGAACACATGGGCCGTATGTTGGCACTGGCCCGCGACTATGGCCGTGCACACGGTTTCAAGGGCACGTTCCTCATCGAGCCCAAACCGATGGAGCCGACCAAGCACCAGTACGATCAGGATACGGAAACCGTCATCGGCTTCCTGCGCCGCCATGGCCTCGACAAGGACTTCAAGGTCAACATCGAGGTGAACCATGCTACCCTGGCGGGCCACACCTTCGAGCACGAGCTGGCTTGCGCCGTCGACCACGGCATGCTGGGCAGCATCGACGCCAACCGGGGTGATGCCCAGAACGGCTGGGACACCGACCAGTTCCCGATCGATAACTATGAGCTGACGCTGGCCATGCTCCAGATCATCCGCAACGGCGGCCTGGCACCCGGCGGCTCGAACTTCGATGCGAAGCTGCGTCGCAACTCCACCGATCCGGAAGATATCTTCATCGCGCACATCAGCGCCATGGATGCCATGGCCCGCGCCCTGGTCAATGCTGTCGCCATTCTCGAGGAATCGCCCATCCCGGCCATGGTCAGGGAACGTTACGCCTCGTTCGACAGCGGAAAGGGCAGGGAATATGAGGAAGGCAGGCTGTCTCTCGAAGACATCGTGGCCTATGCCAAAGCCCACGGCGAACCGAAACAGATTTCCGGCAAGCAGGAACTCTACGAAACCATCGTGGCTCTCTATTGCAAGTAG 5586MI193_004Prevotella Amino 100MTKEYFPTIGKIPFEGPESKNPLAFHYYEPDRLVMGKKMKDWLRFAMAWWHTLGQASGDQ AcidFGGQTRHYAWDDPDCPYARAKAKADAGFEIMQKLGIEFFCFHDIDLVEDADEIAEYEARMKDITDYLLVKMKETGIKNLWGTANVFGHKRYMNGAATNPDFDVLARAAVQIKNAIDATIKLGGQNYVFWGGREGYQTLLNTQMQREKEHMGRMLALARDYGRAHGFKGTFLIEPKPMEPTKHQYDQDTETVIGFLRRHGLDKDFKVNIEVNHATLAGHTFEHELACAVDHGMLGSIDANRGDAQNGWDTDQFPIDNYELTLAMLQIIRNGGLAPGGSNFDAKLRRNSTDPEDIFIAHISAMDAMARALVNAVAILEESPIPAMVRERYASFDSGKGREYEEGRLSLEDIVAYAKAHGEPKQISGKQELYETIVALYCK 5586MI195_003 Prevotella DNA 101ATGGCAAAAGAGTATTTCCCGCAGATCGGAAAGATCGGCTTTGAGGGTCCTGCAAGCAAGAACCCGCTGGCATTCCATTATTATGACGCCGAGCGCGTGGTGATGGGTAAACCCATGAAAGACTGGTTTAAATTCGCCCTCGCGTGGTGGCACAGCCTCGGCCAGGCCTCCGGCGACCCGTTCGGCGGCCAGACCCGCTCCTACGAGTGGGACAAGGGCGAATGCCCCTACTGCCGCGCCCGCGCCAAGGCGGACGCCGGCTTCGAGATCATGCAAAAGCTCGGCATCGGCTATTTCTGCTTCCACGACGTCGACCTCATCGAAGACACGGACGACATCGCCGAATATGAGGCCCGCCTCAAGGACATCACGGACTACCTGCTCGAAAGGATGCAGGAAACCGGCATCAAGAACCTCTGGGGCACGGCCAATGTCTTCGGTCACAAGCGCTACATGAACGGCGCCGGCACCAATCCGCAGTTCGACATCGTCGCCCGCGCTGCCGTCCAGATCAAGAACGCCCTCGACGCCACCATCAAGCTCGGTGGCTCGAACTACGTCTTCTGGGGCGGCCGCGAAGGTTATTACACGCTGCTCAACACCCAGATGCAGCGCGAGAAAGACCACCTCGCCAAGCTCCTCACCGCCGCCCGCGACTATGCCCGCGCCAAGGGCTTCCAGGGCACCTTCCTGATCGAGCCCAAGCCGATGGAGCCGACCAAGCACCAGTACGATGTCGACACGGAGACTGTAATCGGATTCCTCCGCGCCAACGGACTGGACAAGGACTTCAAGGTCAACATCGAGGTCAACCACGCCACCCTCGCCGGCCATACCTTCGAGCATGAGCTGACCGTCGCCCGCGAGAACGGATTCCTCGGCAGCATCGACGCCAACCGCGGTGACGCCCAGAACGGCTGGGACACCGACCAGTTCCCCGTGGACGCCTACGACCTCACCCAGGCCATGATGCAGGTGCTCCTGAACGGCGGTTTCGGCAACGGCGGCACCAATTTCGACGCCAAGCTCCGTCGCAGCTCCACCGATCCCGAGGACATCTTCATCGCCCACATCAGCGCGATGGACGCCATGGCCCACGCCCTGCTGAACGCCGCGGCCATTCTCGAGGAGAGCCCGCTGCCCGCGATGGTCAAGGAGCGTTACGCCTCCTTCGACAGCGGTCTCGGCAAGCAGTTCGAGGAGGGAAAGGCCACGCTGGAGGACCTCTACGACTACGCCAAGGCCCATGGCGAGCCCGTCGCCGCCTCCGGCAAGCAGGAACTGTGTGAAACTTACCTGAATCTGTATGCAAAGTAA 5586MI195_003Prevotella Amino 102MAKEYFPQIGKIGFEGPASKNPLAFHYYDAERVVMGKPMKDWFKFALAWWHSLGQASGDP AcidFGGQTRSYEWDKGECPYCRARAKADAGFEIMQKLGIGYFCFHDVDLIEDTDDIAEYEARLKDITDYLLERMQETGIKNLWGTANVFGHKRYMNGAGTNPQFDIVARAAVQIKNALDATIKLGGSNYVFWGGREGYYTLLNTQMQREKDHLAKLLTAARDYARAKGFQGTFLIEPKPMEPTKHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANRGDAQNGWDTDQFPVDAYDLTQAMMQVLLNGGEGNGGTNEDAKLRRSSTDPEDIFIAHISAMDAMAHALLNAAAILEESPLPAMVKERYASFDSGLGKQFEEGKATLEDLYDYAKAHGEPVAASGKQELCETYLNLYAK 5586MI196_003 Prevotella DNA 103ATGACAAAAGAGTATTTCCCTACCATCGGCAAGATCCCCTTTGAGGGACCCGAGAGCAAAAACCCCCTCGCTTTTCATTACTATGAGCCCGACCGCCTGGTCATGGGCAAGAAGATGAAAGACTGGCTGCGTTTCGCCATGGCCTGGTGGCACACCCTGGGCCAGGCCTCCGGCGACCAGTTTGGCGGCCAGACCCGCCACTATGCCTGGGATGATCCGGATTGCCCGTATGCACGTGCCAAAGCCAAGGCCGACGCCGGTTTCGAAATCATGCAGAAACTGGGCATTGAATTCTTCTGCTTCCACGACATCGACCTGATCGAGGATACCGATGACATCGTCGAGTATGAGGCCCGGATGAAGGACATCACCGACTATCTGCTGGTCAAGATGAAAGAGACCGGCATCAAGAATCTCTGGGGAACGGCCAACGTATTCGGGCACAAGCGCTATATGAACGGCGCTGCCACCAACCCCGATTTCGACGTGCTGGCCCGTGCCGCCGTCCAGATCAAGAACGCCATCGACGCCACCATCAAGCTGGGCGGCCAGAATTATGTGTTCTGGGGCGGGCGTGAAGGCTACCAGAGCCTGCTCAATACCCAGATGCAGCGCGAAAAGGAACACATGGGCCGTATGTTGGCACTAGCCCGCGACTATGGCCGTGCACACGGTTTCAAGGGCACGTTCCTCATCGAGCCCAAACCGATGGAGCCGACCAAGCACCAGTACGATCAGGATACGGAGACCGTCATCGGTTTTCTGCGCCGCCATGGCCTCGACAAGGACTTCAAGGTCAACATCGAGGTGAACCATGCTACCCTGGCGGGCCACACCTTCGAGCACGAGCTGGCCTGCGCCGTCGACCACGGCATGCTGGGCAGTATTGACGCCAACCGCGGTGACGCCCAGAACGGCTGGGACACCGACCAGTTCCCGATCGATAACTATGAGCTGACGCTGGCCATGCTCCAGATCATCCGCAACGGCGGCCTGGCACCCGGCGGCTCGAACTTCGATGCGAAGCTGCGTCGCAACTCCACCGATCCGGAAGATATCTTCATCGCGCACATCAGCGCCATGGATGCCATGGCCCGCGCCCTGGTCAACGCTGTCGCCATTCTTGAGGAATCGCCCATTCCGGACATGGTCAAGGAGCGCTACGCTTCGTTCGACAGCGGAAAAGGCAGGGAGTACGAAGAGGGGAAACTTTCCTTCGAGGACCTCGTGGCCTATGCCAAAGCCCACGGCGAACCGAAACAGATTTCCGGCAAGCAGGAACTCTACGAAACCATCGTGGCTCTCTATTGCAAGTAG 5586MI196_003Prevotella Amino 104MTKEYFPTIGKIPFEGPESKNPLAFHYYEPDRLVMGKKMKDWLRFAMAWWHTLGQASGDQ AcidFGGQTRHYAWDDPDCPYARAKAKADAGFEIMQKLGIEFFCFHDIDLIEDTDDIVEYEARMKDITDYLLVKMKETGIKNLWGTANVFGHKRYMNGAATNPDFDVLARAAVQIKNAIDATIKLGGQNYVFWGGREGYQSLLNTQMQREKEHMGRMLALARDYGRAHGFKGTFLIEPKPMEPTKHQYDQDTETVIGFLRRHGLDKDFKVNIEVNHATLAGHTFEHELACAVDHGMLGSIDANRGDAQNGWDTDQFPIDNYELTLAMLQIIRNGGLAPGGSNFDAKLRRNSTDPEDIFIAHISAMDAMARALVNAVAILEESPIPDMVKERYASFDSGKGREYEEGKLSFEDLVAYAKAHGEPKQISGKQELYETIVALYCK 5586MI197_003 Prevotella DNA 105ATGACAAAAGAGTATTTCCCTACCATCGGCAAGATCCCCTTTGAGGGACCCGAGAGCAAAAACCCCCTCGCTTTTCATTACTATGAGCCCGACCGCCTGGTCATGGGCAAGAAGATGAAAGACTGGCTGCGTTTCGCCATGGCCTGGTGGCACACCCTGGGCCAGGCCTCCGGCGACCAGTTTGGCGGCCAGACCCGCCACTATGCCTGGGATGATCCGGATTGCCCGTATGCACGTGCCAAAGCCAAGGCCGACGCCGGTTTCGAAATCATGCAGAAACTGGGCATTGAATTCTTCTGCTTCCACGACATCGACCTGATCGAGGATACCGATGACATCGTCGAGTATGAGGCCCGGATGAAGGACATCACCGACTATCTGCTGGTCAAGATGAAAGAGACCGGCATCAAGAATCTCTGGGGAACGGCCAACGTATTCGGGCACAAGCGCTATATGAACGGCGCTGCCACCAACCCCGATTTCGACGTGCTGGCCCGTGCCGCCGCCCAGATCAAGAACGCCATCGACGCCACCATCAAGCTGGGCGGCCAGAATTATGTGTTCTGGGGCGGGCGTGAAGGCTACCAGAGCCTGCTCAATACCCAGATGCAGCGCGAAAAGGAACACATGGGCCGTATGTTGGCACTAGCCCGCGACTATGGCCGTGCACACGGTTTCAAGGGCACGCTCCTCATCGAGCCCAAACCGATGGAGCCGACCAAGCACCAGTACGATCAGGATACGGAGACCGTCATCGGTTTTCTGCGCCGCCATGGCCTCGACAAGGACTTCAAGGTCAACATCGAGGTGAACCATGCTACCCTGGCGGGCCACACCTTCGAGCACGAGCTGGCCTGCGCCGTCGACCACGGCATGCTGGGCAGTATTGACGCCAACCGCGGTGACGCCCAGGACGGCTGGGACACCGACCAGTTCCCGATCGATAACTATGAGCTGACGCTGGCCATGCTCCAGATCATCCGCAACGGCGGCCTGGCACCCGGCGGCTCGAACTTCGATGCGAAGCTGCGTCGCAACTCCACCGATCCGGAAGATATCTTCATCGCGCACATCAGCGCCATGGATGCCATGGCCCGCGCCCTGGTCAACGCTGTCGCCATTCTTGAGGAATCGCCCATTCCGGACATGGTCAAGGAGCGCTACGCTTCGTTCGACAGCGGAAAAGGCAGGGAGTACGAAGAGGGGAAACTTTCCTTCGAGGACCTCGTGGCCTATGCCAAAGCCCACGGCGAACCGAAACAGATTTCCGGCAAGCAGGAACTCTACGAAACCATCGTGGCTCTCTATTGCAAGTAG 5586MI197_003Prevotella Amino 106MTKEYFPTIGKIPFEGPESKNPLAFHYYEPDRLVMGKKMKDWLRFAMAWWHTLGQASGDQ AcidFGGQTRHYAWDDPDCPYARAKAKADAGFEIMQKLGIEFFCFHDIDLIEDTDDIVEYEARMKDITDYLLVKMKETGIKNLWGTANVFGHKRYMNGAATNPDFDVLARAAAQIKNAIDATIKLGGQNYVFWGGREGYQSLLNTQMQREKEHMGRMLALARDYGRAHGFKGTLLIEPKPMEPTKHQYDQDTETVIGFLRRHGLDKDFKVNIEVNHATLAGHTFEHELACAVDHGMLGSIDANRGDAQDGWDTDQFPIDNYELTLAMLQIIRNGGLAPGGSNFDAKLRRNSTDPEDIFIAHISAMDAMARALVNAVAILEESPIPDMVKERYASFDSGKGREYEEGKLSFEDLVAYAKAHGEPKQISGKQELYETIVALYCK 5586MI199_003 Prevotella DNA 107ATGACAAAAGAGTATTTCCCTACCATCGGCAAGATCCCCTTTGAGGGACCCGAGAGCAAAAACCCCCTCGCTTTTCATTACTATGAGCCCGACCGCCTGGTCATGGGCAAGAAGATGAAAGACTGGCTGCGTTTCGCCATGGCCTGGTGGCACACCCTGGGCCAGGCCTCCGGCGACCAGTTTGGCGGCCAGACCCGCCACTATGCCTGGGATGATCCGGATTGCCCGTATGCACGTGCCAAAGCCAAGGCCGACGCCGGTTTCGAAATCATGCAGAAACTGGGCATTGAATTCTTCTGCTTCCACGACATCGACCTGATCGAGGATACCGATGACATCGTCGAGTATGAGGCCCGGATGAAGGACATCACCGACTATCTGCTGGTCAAGATGAAAGAGACCGGCATCAAGAATCTCTGGGGAACGGCCAACGTATTCGGGCACAAGCGCTATATGAACGGCGCTGCCACCAACCCCGATTTCGACGTGCTGGCCCGTGCCGCCGTCCAGATCAAGAACGCCATCGACGCCACCATCAAGCTGGGCGGCCAGAATTATGTGTTCTGGGGCGGGCGTGAAGGCTACCAGAGCCTGCTCAATACCCAGATGCAGCGCGAAAAGGAACACATGGGCCGTATGTTGGCACTAGCCCGCGACTATGGCCGTGCACACGGTTTCAAGGGCACGTTCCTCATCGAGCCCAAACCGATGGAGCCGACCAAGCACCAGTACGATCAGGATACGGAGACCGTCATCGGTTTTCTGCGCCGCCATGGCCTCGACAAGGACTTCAAGGTCAACATCGAGGTGAACCATGCTACCCTGGCGGGCCACACCTTCGAGCACGAGCTGGCCTGCGCCGTCGACCACGGCATGCTGGGCAGTATTGACGCCAACCGCGGTGACGCCCAGAACGGCTGGGACACCGACCAGTTCCCGATCGATAACTATGAGCTGACGCTGGCCATGCTCCAGATCATCCGCAACGGCGGCCTGGCACCCGGCGGCTCGAACTTCGATGCGAAGCTGCGTCGCAACTCCACCGATCCGGAAGATGTCTTCATCGCGCACATCAGCGCCATGGATGCCATGGCCCGCGCCCTGGTCAACGCTGTCGCCATTCTTGAGGAATCGCCCATTCCGGACATGGTCAAGGAGCGCTACGCTTCGTTCGACAGCGGAAAAGGCAGGGAGTACGAAGAGGGGAAACTTTCCTTCGAGGACCTCGTGGCCTATGCCAAAGCCCACGGCGAACCGAAACAGATTTCCGGCAAGCAGGAACTCTACGAAACCATCGTGGCTCTCTATTGCAAGTAG 5586MI199_003Prevotella Amino 108MTKEYFPTIGKIPFEGPESKNPLAFHYYEPDRLVMGKKMKDWLRFAMAWWHTLGQASGDQ AcidFGGQTRHYAWDDPDCPYARAKAKADAGFEIMQKLGIEFFCFHDIDLIEDTDDIVEYEARMKDITDYLLVKMKETGIKNLWGTANVFGHKRYMNGAATNPDFDVLARAAVQIKNAIDATIKLGGQNYVFWGGREGYQSLLNTQMQREKEHMGRMLALARDYGRAHGFKGTFLIEPKPMEPTKHQYDQDTETVIGFLRRHGLDKDFKVNIEVNHATLAGHTFEHELACAVDHGMLGSIDANRGDAQNGWDTDQFPIDNYELTLAMLQIIRNGGLAPGGSNFDAKLRRNSTDPEDVFIAHISAMDAMARALVNAVAILEESPIPDMVKERYASFDSGKGREYEEGKLSFEDLVAYAKAHGEPKQISGKQELYETIVALYCK 5586MI200_003 Prevotella DNA 109ATGGCAAAAGAGTATTTCCCGACAATCGGAAAGATCCCCTTCGAGGGCGTTGAGAGCAAGAATCCCCTTGCTTTCCATTATTATGACGCCGAGCGCGTGGTCATGGGCAAGCCCATGAAGGACTGGTTCAAGTTCGCGATGGCCTGGTGGCACACCCTGGGCCAGGCTTCCGCGGACCCGTTCGGCGGCCAGACCCGCTCCTACGAGTGGGACAAGGGCGAGTGCCCCTACTGCCGCGCCCGCGCCAAGGCTGACGCCGGCTTCGAGATCATGCAGAAGCTCGGAATCGGCTACTATTGCTTCCACGACATCGACCTGGTGGAGGACACCGAGGACATCGCCGAATACGAGGCCCGCATGAAGGACATCACCGACTACCTCGTCGAGAAGCAGAAGGAGACCGGCATCAAGAACCTCTGGGGCACCGCGAACGTGTTCGGCAACAAGCGCTACATGAACGGCGCCGCCACGAACCCGCAGTTCGACATCGTCGCCCGCGCGGCCCTGCAGATCAAGAACGCGATCGATGCCACCATCAAGCTCGGCGGCACCGGCTACGTGTTCTGGGGCGGCCGGGAAGGCTACTACACCCTGCTGAACACCCAGATGCAGCGCGAGAAGGACCACCTCGCCAAGATGCTCACCGCCGCCCGCGACTACGCCCGCGCCAACGGCTTCAAGGGCACCTTCCTCATCGAGCCCAAGCCGATGGAGCCCACCAAGCACCAATACGACGTGGACACGGAGACCGTGATCGGCTTCCTCCGCGCCAATGGCCTGGACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCCACCCTCGCCGGCCACACCTTCGAGCACGAGCTCACCGTGGCCGTTGACAACGGCTTCCTCGGCAGCATCGACGCCAACCGCGGCGACGCCCAGAACGGCTGGGATACCGACCAGTTCCCGGTGGATCCGTACGATCTCACCCAGGCGATGATCCAGATCATCCGCAACGGCGGCTTCAAGGACGGCGGCACCAACTTCGACGCCAGGCTCCGCCGCTCTTCCACCGACCCGGAGGACATCTTCATCGCCCACATCAGCGCGATGGACGCCATGGCCCACGCCCTGCTGAACGCCGCCGCCGTCATCGAGGAGAGCCCGCTCTGCGAGATGGTCGCCAAGCGTTACGCTTCCTTCGACAGCGGCCTCGGCAAAAAGTTCGAGGAAGGCAAGGCCACCCTCGAGGAACTCTACGAGTATGCCAAGGCGAACGGTGAGGTCAAGGCCGAATCCGGCAAGCAGGAGCTCTACGAGACCCTTCTGAACCTCTACGCGAAATAG 5586MI200_003Prevotella Amino 110MAKEYFPTIGKIPFEGVESKNPLAFHYYDAERVVMGKPMKDWFKFAMAWWHTLGQASADP AcidFGGQTRSYEWDKGECPYCRARAKADAGFEIMQKLGIGYYCFHDIDLVEDTEDIAEYEARMKDITDYLVEKQKETGIKNLWGTANVFGNKRYMNGAATNPQFDIVARAALQIKNAIDATIKLGGTGYVFWGGREGYYTLLNTQMQREKDHLAKMLTAARDYARANGFKGTFLIEPKPMEPTKHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVAVDNGFLGSIDANRGDAQNGWDTDQFPVDPYDLTQAMIQIIRNGGFKDGGTNFDARLRRSSTDPEDIFIAHISAMDAMAHALLNAAAVIEESPLCEMVAKRYASFDSGLGKKFEEGKATLEELYEYAKANGEVKAESGKQELYETLLNLYAK 5586MI203_003 Prevotella DNA 111ATGGCACAAGCGTATTTTCCTACCATCGGGAAAATCCCCTTCGAGGGACCCGAAAGCAAGAATCCCCTGGCATTCCATTATTATGAGCCCGACCGCCTGGTCCTGGGCAAGAAGATGAAGGACTGGCTGCGTTTCGCCATGGCCTGGTGGCACACGCTGGGCCAGGCTTCCGGCGACCAGTTCGGCGGCCAGACCCGCCACTACGCCTGGGACGAGCCCGCCACGCCCCTGGAACGGGCCAAGGCCAAGGCGGATGCCGGTTTCGAGATCATGCAGAAACTGGGCATCGAATTCTTCTGCTTCCACGATGTGGACCTCATCGAAGAGGGCGCCACGATCGAGGAATACGAGCAGCGGATGCAGCAGATCACGGATTATCTGCTGGTCAAGATGAAAGAGACCGGCATCCGCAACCTCTGGGGTACGGCCAACGTGTTCGGACACGAGCGCTACATGAACGGCGCGGCCACGAACCCCGATTTCGATGTCGTGGCCCGCGCGGCCGTGCAGATCAAGACGGCCATCGACGCCACCATCAAGTTGGGCGGCGAGAACTATGTGTTCTGGGGCGGCCGGGAAGGCTATATGAGCCTGCTCAATACGCAGATGCACCGCGAGAAGCTGCATCTGGGCAAGATGCTCGCCGCGGCCCGCGACTACGGACGCGCCCACGGCTTCAAGGGGACCTTCCTCATCGAACCCAAGCCGATGGAACCCACCAAGCATCAGTATGACCAGGATACGGAGACGGTCATCGGTTTCCTGCGCCGCTACGGCCTGGACGAAGACTTCAAGGTGAACATCGAGGTCAACCACGCTACGCTGGCCGGCCATACCTTCGAACACGAACTGGCCACGGCGGTCGATGCCGGCCTGCTGGGCAGCATCGACGCCAACCGCGGCGACGCCCAGAACGGCTGGGATACCGACCAGTTCCCGATCGACAACTACGAACTGACCCTGGCGATGCTGCAGGTCATCCGCAACGGCGGTCTGGCCCCGGGCGGCTCGAATTTCGATGCCAAGCTCCGCCGGAACTCCACCGATCCGGAAGACATCTTCATTGCCCACATCAGCGCGATGGATGCGATGGCGCGGGCCCTGCTCAATGCGGCCGCCCTCTGCGAGACGTCCCCGATTCCGGCGATGGTCAAGGCGCGTTACGCTTCGTTCGACAGCGGCGCCGGCAAGGATTTCGAAGAGGGAAGGATGACGCTGGAAGACCTCGTGGCCTATGCCAGGACCCACGGCGAGCCGAAGCGGACCTCGGGCAAGCAGGAACTCTATGAGACCCTCGTGGCGCTTTATTGCAAATAG 5586MI203_003Prevotella Amino 112MAQAYFPTIGKIPFEGPESKNPLAFHYYEPDRLVLGKKMKDWLRFAMAWWHTLGQASGDQ AcidFGGQTRHYAWDEPATPLERAKAKADAGFEIMQKLGIEFFCFHDVDLIEEGATIEEYEQRMQQITDYLLVKMKETGIRNLWGTANVFGHERYMNGAATNPDFDVVARAAVQIKTAIDATIKLGGENYVFWGGREGYMSLLNTQMHREKLHLGKMLAAARDYGRAHGFKGTFLIEPKPMEPTKHQYDQDTETVIGFLRRYGLDEDFKVNIEVNHATLAGHTFEHELATAVDAGLLGSIDANAGDAQNGWDTDQFPIDNYELTLAMLQVIRKGGLAPGGSNFDAKLRRNSTDPEDIFIAHISAMDAMARALLNAAALCETSPIPAMVKARYASFDSGAGKDFEEGRMTLEDLVAYARTHGEPKRTSGKQELYETLVALYCK 5586MI205_004 Prevotella DNA 113ATGACCAACGAGTATTTTCCCGGAATCGGTGTGATTCCGTTTGAAGGACAGGAAAGCAAGAATCCCCTGGCTTTCCATTATTATGACGCCAACCGCGTAGTGATGGGCAAACCCATGAAGGAATGGTTCAAATTTGCCATGGCCTGGTGGCATACGCTGGGGCAGGCATCGGCCGATCCCTTCGGCGGACAGACCCGCTCCTACGCATGGGACAAGGGCGAGTGCCCTTACTGCCGTGCCCGCCAGAAGGCCGACGCCGGCTTTGAACTGATGCAGAAGCTGGGAATCGGCTATTTCTGCTTCCACGATGTGAATATCATCGAGGACTGCGAGGACATTGCCGAGTATGAGGCCCGTATGAAGGACATCACGGACTATCTGCTGGTGAAGATGAAGGAAACGGGCATCAAGAATCTGTGGGGCACGGCCAACGTCTTCGGCCACAAGCGCTATATGAACGGCGCCGCCACCAACCCGCAATTCGACGTGGTAGCCCGCGCTGCGGTCCAGATCAAGAACGCCCTGGACGCCACCATCAAGCTGGGCGGCAGCAATTATGTGTTCTGGGGCGGCCGGGAAGGCTACTACACCCTTTTGAACACGCAGATGCAGCGGGAGAAGGACCACCTGGCCCAGATGCTCAAGGCGGCCCGCGACTATGCCCGCGGCAAGGGATTCAAGGGCACGTTCCTCATTGAGCCCAAGCCCATGGAGCCCACCAAGCACCAGTACGACGTAGATACGGAGACCGTGATTGGTTTCCTGCGCGCCAACGGGCTGGACAAGGACTTCAAGGTGAATATCGAAGTGAACCACGCCACCCTGGCCGGCCATACCTTCGAGCACGAGCTCACCGTGGCCCGCGAAAACGGCTTCCTGGGCAGCATCGACGCCAACCGCGGAGACGCCCAGAACGGCTGGGATACAGACCAGTTCCCCGTGGACGCCTTTGACCTCACCCAGGCCATGATGCAGGTCCTGCTCAACGGCGGATTCGGCAACGGCGGCACCAACTTCGACGCCAAACTGCGCCGTTCCTCCACGGATCCCGAGGACATCTTCATCGCCCACATCAGCGCCATGGACGCCATGGCCCACGCCCTCCTGAACGCCGCCGCCATCCTGGAAGAGAGCCCCATGCCGGGCATGGTGAAGGAGCGCTACGCTTCCTTCGACAATGGCCTTGGCAAGAAGTTCGAGGAAGGAAAGGCCACGCTGGAAGAGCTGTACGACTATGCCAAGAAGAACGGCGAGCCTGTGGCCGCTTCCGGAAAGCAGGAACTGTACGAAACGCTGCTGAACCTGTACGCCAAGTAA 5586MI205_004Prevotella Amino 114MTNEYFPGIGVIPFEGQESKNPLAFHYYDANRVVMGKPMKEWFKFAMAWWHTLGQASADP AcidFGGQTRSYAWDKGECPYCRARQKADAGFELMQKLGIGYFCFHDVNIIEDCEDIAEYEARMKDITDYLLVKMKETGIKNLWGTANVFGHKRYMNGAATNPQFDVVARAAVQIKNALDATIKLGGSNYVFWGGREGYYTLLNTQMQREKDHLAQMLKAARDYARGKGFKGTFLIEPKPMEPTKHQYDVDTETVIGFLRANGLDKDEKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANRGDAQNGWDTDQFPVDAFDLTQAMMQVLLNGGFGNGGTNFDAKLRRSSTDPEDIFIAHISAMDAMAHALLNAAAILEESPMPGMVKERYASFDNGLGKKFEEGKATLEELYDYAKKNGEPVAASGKQELYETLLNLYAK 5586MI206_004 Prevotella DNA 115ATGGCAAAAGAGTATTTCCCGACTATCGGCAAGATTCCCTTCGAGGGCGTCGAATCCAAGAACCCGATGGCATTCCACTATTATGACGCGAAACGCGTCGTGATGGGCAAGCCCATGAAGGACTGGCTCAAGTTCGCGATGGCCTGGTGGCACACCCTGGGACAGGCTTCCGGCGACCCGTTCGGCGGCCAGACCCGTTCCTACGAGTGGGACAAGGGCGAGTGCCCCTACTGCCGCGCCAAGGCCAAGGCCGACGCCGGTTTCGAGATCATGCAGAAACTGGGCATCGAGTACTACTGCTTCCATGACATCGACCTGGTGGAGGACACCGAGGACATCGCCGAGTACGAGGCCCGCATGAAGGACATCACCGACTACCTCGTCGAGAAGCAGAAGGAGACCGGTATCAAGAACCTCTGGGGCACGGCCAACGTGTTCGGCAACAAGCGCTACATGAACGGCGCCGCCACGAACCCGCAGTTCGACGTCGTCGCCCGCGCCGCCGTCCAGATCAAGAACGCCATCGACGCCACCATCAAACTCGGCGGCACCTCTTACGTGTTCTGGGGCGGCCGTGAAGGCTACTACACCCTCCTGAACACCCAGATGCAGCGCGAGAAGGACCACCTCGCCAAGATGCTCACCGCCGCCCGCGACTACGCCCGCGCCCACGGCTTCAAGGGCACCTTCCTCATCGAGCCCAAGCCCATGGAGCCCACCAAGCACCAGTACGACGTGGACACGGAGACCGTGATCGGCTTCCTCCGCGCCAACGGCCTGGACAAGGACTTCAAGGTCAATATCGAAGTGAACCACGCCACCCTCGCCGGCCACACCTTCGAGCATGAGCTCACCGTGGCGGTCGATAACGGCTTCCTCGGCTCCATCGACGCCAACCGTGGCGACGCCCAGAACGGCTGGGATACCGACCAGTTCCCGGTGGATCCGTACGACCTCACCCAGGCCATGATGCAGATCATCCGCAACGGCGGCTTCAAGGACGGCGGCACCAACTTCGACGCCAAACTCCGCCGCTCCTCCACCGACCCGGAGGACATCTTCATCGCCCACATCAGCGCGATGGACGCCATGGCCCACGCGCTCCTGAACGCCGCCGCCGTCATCGAGGAGAGCCCGCTCTGCAAGATGGTCGAGGAGCGCTACGCTTCCTTCGACAGCGGTCTCGGCAAGCAGTTCGAGGAAGGCAAGGCCACCCTTGAGGACCTCTACGAGTATGCCAAGAAGAACGGCGAGCCCGTCGTCGCTTCCGGCAAGCAGGAGCTCTACGAGACCCTTCTGAACCTCTACGCGAAGTAG 5586MI206_004Prevotella Amino 116MAKEYFPTIGKIPFEGVESKNPMAFHYYDAKRVVMGKPMKDWLKFAMAWWHTLGQASGDP AcidFGGQTRSYEWDKGECPYCRAKAKADAGFEIMQKLGIEYYCFHDIDLVEDTEDIAEYEARMKDITDYLVEKQKETGIKNLWGTANVFGNKRYMNGAATNPQFDVVARAAVQIKNAIDATIKLGGTSYVFWGGREGYYTLLNTQMQREKDHLAKMLTAARDYARAHGEKGTFLIEPKPMEPTKHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVAVDNGFLGSIDANRGDAQNGWDTDQFPVDPYDLTQAMMQIIRNGGFKDGGTNFDAKLRRSSTDPEDIFIAHISAMDAMAHALLNAAAVIEESPLCKMVEERYASFDSGLGKQFEEGKATLEDLYEYAKKNGEPVVASGKQELYETLLNLYAK 5586MI208_003 Prevotella DNA 117ATGTCAACTGAGTATTTCCCTACAATCGGCAAGATTCCCTTCGAGGGACCCGAGAGCAAGAACCCCATGGCCTTCCACTACTATGAACCCGAAAAGTTGGTGATGGGCAAGAAGATGAAGGACTGGCTGCGTTTCGCAATGGCCTGGTGGCACACCCTTGGAGCCGCATCCGGCGACCAGTTCGGCGGACAGACCCGCAGTTACGCCTGGGACAAGGGCGACTGCCCTTACAGCCGCGCCCGCGCCAAGGTCGACGCCGGCTTCGAGATCATGCAGAAGCTCGGCATAGAGTTCTTCTGCTTCCATGACATCGACCTGGTCGAGGATACCGACGACATCGCCGAGTATGAAGCCCGGATGAAAGACATCACGGACTATCTGCTGGAAAAGATGGAGGCTACCGGCATCAAGAACCTCTGGGGCACGGCCAATGTCTTCGGTCACAAGCGTTATATGAACGGTGCAGCCACAAACCCCGATTTCGCAGTGGTCGCAAGGGCGGCCGTGCAGATCAAGAACGCCATCGACGCCACCATCAAGCTGGGTGGTGAGAACTATGTGTTCTGGGGTGGACGCGAGGGTTATATGAGCCTGCTCAACACCCAGATGCAGAGGGAGAAGGAACACCTTGCCAAGATGCTCACCGCCGCACGTGACTATGCACGCGCCAAAGGTTTCAAGGGCACGTTCCTCATCGAACCCAAGCCGATGGAACCCACCAAGCACCAGTATGACCAGGATACCGAGACCGTTATCGGATTCCTCCGCAGCCACGGCCTGGACAAGGACTTCAAGGTCAACATCGAGGTGAACCACGCCACCCTGGCGGGCCATACCTTCGAGCACGAACTGGCCACCGCCGTCGACAACGGCATGCTCGGCAGCATCGACGCCAACCGCGGAGACGCCCAGAACGGCTGGGACACCGACCAGTTCCCGATCGACAACTTCGAGCTCACGCTTGCCATGATGCAGATAATCCGCAACGGCGGCCTGGCACCGGGCGGTTCGAACTTCGACGCAAAGCTGCGCCGCAATTCCACCGATCCCGAGGACATCTTCATCGCCCACATCAGCGCGATGGACGCCATGGCCCGCGCCCTCGTCAACGCCGCCGCCATCCTCGGCGAGTCGCCCGTTCCGGCTATGGTCAAGGACCGCTATGCTTCGTTCGACTGCGGCAAGGGCAAGGACTTCGAAGACGGCAAACTGACTCTCGAAGACATCGTCGCCTACGCCAGGGAGAATGGCGAGCCGAAACAGATTTCCGGCAAGCAGGAACTCTACGAAACTATCGTCGCTCTTTACTGCAAGTAA 5586MI208_003Prevotella Amino 118MSTEYFPTIGKIPFEGPESKNPMAFHYYEPEKLVMGKKMKDWLRFAMAWWHTLGAASGDQ AcidFGGQTRSYAWDKGDCPYSRARAKVDAGFEIMQKLGIEFFCFHDIDLVEDTDDIAEYEARMKDITDYLLEKMEATGIKNLWGTANVFGHKRYMNGAATNPDFAVVARAAVQIKNAIDATIKLGGENYVFWGGREGYMSLLNTQMQREKEHLAKMLTAARDYARAKGFKGTFLIEPKPMEPTKHQYDQDTETVIGFLRSHGLDKDFKVNIEVNHATLAGHTFEHELATAVDNGMLGSIDANRGDAQNGWDTDQFPIDNFELTLAMMQIIRNGGLAPGGSNFDAKLRRNSTDPEDIFIAHISAMDAMARALVNAAAILGESPVPAMVKDRYASFDCGKGKDFEDGKLTLEDIVAYARENGEPKQISGKQELYETIVALYCK 5586MI210_002 Prevotella DNA 119ATGTCATATTTTCCTACTATCGGTAACATCCCCTTTGAGGGTGTAGAGAGCAAGAATCCCCTTGCCTTCCATTATTATGACGCTTCCCGCGTAGTTATGGGCAAGCCCATGAAGGAGTGGCTCAAGTTTGCCATGGCCTGGTGGCACACGCTGGGTCAGGCATCGGCCGACCCTTTCGGCGGACAAACCCGCAGCTATGCCTGGGACAAAGGCGAGTGCCCCTACTGCCGTGCCCGTGCCAAGGCCGACGCCGGCTTCGAGCTCATGCAGAAACTGGGCATCGAGTATTTCTGCTCCCACGACATTGACCTCATCGAGGACTGCGACGACATTGCAGAGTACGAGGCCCGTCTGAAGGACATTACGGACTACCTCCTGGAGAAGATGAAGAAGACCGGTATCAAGAACCTGTGGGGTACGGCCAATGTGTTCGGTAACAAGCGTTACATGAACGGTGCTGCTACCAACCCTCAGTTTGACGTTGTGGCCCGCGCTGCCGTCCAGATCAAGAACGCCATTGACGCTACCATCAAGCTGGGCGGTTCCAACTATGTGTTCTGGGGTGGCCGTGAGGGTTACTACACGCTTCTGAACACCCAGATGCAGCGTGAGAAGAATCACCTGGCTGCCATGCTCAAGGCTGCCCGCGACTATGCCCGCGCCAACGGTTTCAAGGGCACCTTCCTCATTGAGCCCAAGCCCATGGAGCCCACCAAGCACCAGTACGACGTAGACACGGAGACCGTGATTGGATTCCTCCGCGCCAACGGTCTGGAGAAGGACTTCAAGGTGAACATTGAGGTGAACCACGCTACTCTTGCCGGTCACACCTTCGAGCACGAGCTCACCGTGGCCCGTGAGAACGGCTTCCTGGGTTCCATTGACGCCAACCGCGGAGATGCCCAGAACGGCTGGGACACCGACCAGTTCCCGGTAGATGCCTTTGACCTCACCCAGGCCATGATGCAGATTCTCCTCAACGGAGGCTCCGGCAATGGCGGTACCAACTTTGACGCCAAGCTGCGCCGTTCCTCCACCGACCCCGAGGACATCTTCATCGCGCACATCAGCGCCATGGATGCCATGGCTCACGCCCTGCTCAATGCAGCTGCCGTGCTGGAGGAGAGCCCGCTTTGCAAGATGGTCAAGGAGCGTTACGCTTCCTTCGACAGCGGTCTTGGCAAGCAGTTCGAGGAAGGAAAGGCTACGCTGGAAGATCTGTATGCCTATGCCGTCAAGAACGGTGAGCCCGTGGTGGCTTCCGGCAAGCAGGAACTGTACGAAACCTTCCTGAACCTCTATGCAAAATGGTAA 5586MI210_002Prevotella Amino 120MSYFPTIGNIPFEGVESKNPLAFHYYDASRVVMGKPMKEWLKFAMAWWHTLGQASADPFG AcidGQTRSYAWDKGECPYCRARAKADAGFELMQKLGIEYFCSHDIDLIEDCDDIAEYEARLKDITDYLLEKMKKTGIKNLWGTANVFGNKRYMNGAATNPQFDVVARAAVQIKNAIDATIKLGGSNYVFWGGREGYYTLLNTQMQREKNHLAAMLKAARDYARANGFKGTFLIEPKPMEPTKHQYDVDTETVIGFLRANGLEKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANRGDAQNGWDTDQFPVDAFDLTQAMMQILLNGGSGNGGTNFDAKLRRSSTDPEDIFIAHISAMDAMAHALLNAAAVLEESPLCKMVKERYASFDSGLGKQFEEGKATLEDLYAYAVKNGEPVVASGKQELYETFLNLYAKW 5586MI212_002 Prevotella DNA 121ATGTCAACTGAGTATTTCCCTACAATCGGCAAGATTCCCTTCGAGGGACCCGAGAGCAAGAACCCCATGGCCTTCCACTACTATGAACCCGAAAAGTTGGTGATGGGCAAGAAGATGAAGGACTGGCTGCGTTTCGCAATGGCCTGGTGGCACACCCTTGGAGCCGCATCCGGCGACCAGTTCGGCGGACAGACCCGCAGTTACGCCTGGGACAAGGGCGACTGCCCTTACAGCCGCGCCCGCGCCAAGGTCGACGCCGGCTTCGAGATCATGCAGAAGCTCGGCATAGAGTTCTTCTGCTTCCATGACATCGACCTGGTCGAGGATACCGACGACATCGCCGAGTATGAAGCCCGGATGAAAGACATCACGGACTATCTGCTGGAAAAGATGGAGGTTACCGGCATCAAGAACCTCTGGGGCACGGCCAATGTCTTCGGTCACAAGCGTTATATGAACGATGCAGCCACAAACCCCGATTTCGCAGTGGTCGCAAGGGCGGCCGTGCAGATCAAGAACGCCATCGACGCCACCATCAAGCTGGGTGGTGAGAACTATGTGTTCTGGGGTGGACGCGAGGGTTATATGAGCCTGCTCAACACCCAGATGCAGAGGGAGAAGGAACACCTTGCCAAGATGCTCACCGCCGCACGTGACTATGCACGCGCCAAAGGTTTCAAGGGCACGTTCCTCATCGAACCCGAGCCGATGGAACCCACCAAGCACCAGTATGACCAGGATACCGAGACCGTTATCGGATTCCTCCGCAGCCACGGCCTGGACAAGGACTTCAAGGTCAACATCGAGGTGAACCACGCCACCCTGGCGGGCCATACCTTCGAGCACGAACTGGCCACCGCCGTCGACAACGGCATGCTCGGCAGCATCGACGCCAACCGCGGAGACGCCCAGAACGGCTGGGACACCGACCAGTTCCCGATCGACAACTTCGAGCTCACGCTTGCCATGATGCAGATAATCCGCAACGGCGGCCTGGCACCGGGCGGTTCGAACTTCGACGCAAAGCTGCGCCGCAATTCCACCGATCCCGAGGACATCATCATCGCCCACATCAGCGCGATGGACGCCATGGCCCGCGCCCTCGTCAACGCCGCCGCCATCCTCGGCGAGTCGCCCGTTCCGGCTATGGTCAAGGACCGCTATGCTTCGTTCGACTGCGGCAAGGGCAAGGACTTCGAAGACGGCAAACTGACTCTCGAAGACATCGTCGCCTACGCCAGGGAGAATGGCGAGCCGAAACAGATTTCCGGCAAGCAGGAACTCTACGAAACTATCGTCGCTCTTTACTGCAAGTAA 5586MI212_002Prevotella Amino 122MSTEYFPTIGKIPFEGPESKNPMAFHYYEPEKLVMGKKMKDWLRFAMAWWHTLGAASGDQ AcidFGGQTRSYAWDKGDCPYSRARAKVDAGFEIMQKLGIEFFCFHDIDLVEDTDDIAEYEARMKDITDYLLEKMEVTGIKNLWGTANVFGHKRYMNDAATNPDFAVVARAAVQIKNAIDATIKLGGENYVFWGGREGYMSLLNTQMQREKEHLAKMLTAARDYARAKGFKGTFLIEPEPMEPTKHQYDQDTETVIGFLRSHGLDKDFKVNIEVNHATLAGHTFEHELATAVDNGMLGSIDANRGDAQNGWDTDQFPIDNFELTLAMMQIIRNGGLAPGGSNFDAKLRRNSTDPEDIIIAHISAMDAMARALVNAAAILGESPVPAMVKDRYASFDCGKGKDFEDGKLTLEDIVAYARENGEPKQISGKQELYETIVALYCK 5586MI213_003 Prevotella DNA 123ATGACCAACGAGTATTTTCCCGGAATCGGTGTGATTCCGTTTGAAGGACAGGAAAGCAAGAATCCCCTGGCTTTCCATTATTATGACGCCAACCGCGTAGTGATGGGCAAACCCATGAAGGAATGGTTCAAATTTGCCATGGCCTGGTGGCATACGCTGGGGCAGGCATCGGCCGATCCCTTCGGCGGACAGACCCGCTCCTACGCATGGGACAAGGGCGAGTGCCCTTACTGCCGTGCCCGCCAGAAGGCCGACGCCGGCTTTGAACTGATGCAGAAGCTGGGAATCGGCTATTTCTGCTTCCACGATGTGGATATCATCGAGGACTGCGAGGACATTGCCGAGTATGAGGCCCGTATGAAGGACATCACGGACTATCTGCTGGTGAAGATGAAGGAAACGGGCATCAAGAATCTGTGGGGCACGGCCAACGTCTTCGGCCACAAGCGCTATATGAACGGCGCCGCCACCAACCCGCAATTCGACGTGGTAGCCCGCGCTGCGGTCCAGATCAAGAACGCCCTGGACGCCACCATCAAGCTGGGCGGCAGCAATTATGTGTTCTGGGGCGGCCGGGAAGGCTACTACACCCTTTTGAACACGCAGATGCAGCGGGAGAAGGACCACCTGGCCCAGATGCTCAAGGCGGCCCGCGACTATGCCCGCGGCAAGGGATTCAAGGGCACGTTCCTCATTGAGCCCAAGCCCATGGAGCCCACCAAGCACCAGTACGACGTAGATACGGAGACCGTGATTGGTTTCCTGCGCGCCAACGGGCTGGACAAGGACTTCAAGGTGAATATCGAAGTGAACCACGCCACCCTGGCCGGCCATACCTTCGAGCACGAGCTCACCGTGGCCCGCGAAAACGGCTTCCTGGGCAGCATCGACGCCAACCGCGGAGACGCCCAGAACGGCTGGGATACAGACCAGTTCCCCGTGGACGCCTTTGACCTCACCCAGGCCATGATGCAGGTCCTGCTCAACGGCGGATTCGGCAACGGCGGCACCAACTTCGACGCCAAACTGCGCCGTTCCTCCACGGATCCCGAGGACATCTTCATCGCCCACATCAGCGCCATGGACGCCATGGCCCACGCCCTCCTGAACGCCGCCGCCATCCTGGAAGAGAGCCCCATGCCGGGCATGGTGAAGGAGCGCTACGCTTCCTTCGACAATGGCCTTGGCAAGAAGTTCGAGGAAGGAAAGGCCACGCTGGAAGAGCTGTACGACTATGCCAAGAAGAACGGCGAGCCTGTGGCCGCTTCCGGAAAGCAGGAACTGTACGAAACGCTGCTGAACCTGTACGCCAAGTAA 5586MI213_003Prevotella Amino 124MTNEYFPGIGVIPFEGQESKNPLAFHYYDANRVVMGKPMKEWFKFAMAWWHTLGQASADP AcidFGGQTRSYAWDKGECPYCRARQKADAGFELMQKLGIGYFCFHDVDIIEDCEDIAEYEARMKDITDYLLVKMKETGIKNLWGTANVFGHKRYMNGAATNPQEDVVARAAVQIKNALDATIKLGGSNYVFWGGREGYYTLLNTQMQREKDHLAQMLKAARDYARGKGFKGTFLIEPKPMEPTKHQYDVDTETVIGELRANGLDKDEKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANRGDAQNGWDTDQFPVDAFDLTQAMMQVLLNGGFGNGGTNFDAKLRRSSTDPEDIFIAHISAMDAMAHALLNAAAILEESPMPGMVKERYASFDNGLGKKFEEGKATLEELYDYAKKNGEPVAASGKQELYETLLNLYAK 5586MI215_003 Prevotella DNA 125ATGGCAAAAGAGTATTTCCCGCAGATCGGAAAGATCGGCTTTGAGGGTCTTGAGAGCAAGAACCCGATGGCATTCCATTATTATGACGCCGAGCGTGTCGTGCTCGGAAAGAAGATGAAGGACTGGCTGAAGTTCGCGATGGCCTGGTGGCATACGCTCGGACAGGCTTCCGGCGACCCATTCGGCGGCCAGACTCGCAGCTATGAGTGGGACAAGGGCGAGTGCCCCTACTGCCGTGCCCGCGCCAAGGCCGACGCCGGCTTCGAGCTCATGCAGAAGCTCGGCATCGAGTACTTCTGCTTCCACGACATCGACCTCATCGAGGACTGCGACGACATCGACGAGTACGAGGCCCGGATGAAGGACATCACCGACTACCTGCTGGAGAAGATGAAGGAGACCGGAATCAAGAATCTCTGGGGAACGGCCAACGTCTTCGGTCACAAGCGCTACATGAACGGCGCCGCTACCAATCCGCAGTTTGAAATCGTCGCCCGCGCTGCCGTCCAGATCAAGAACGCGCTCGACGCCACCATCAAGCTCGGCGGCTCCAACTACGTCTTCTGGGGCGGCCGCGAGGGCTATTACACGCTGCTGAATACCCAGATGCAGCGCGAGAAGGACCATCTCGCCAGGCTCCTTACCGCCGCCCGCGACTATGCGCGCGCCAAGGGGTTCAAGGGGACCTTCCCCATCGAGCCGAAGCCGATGGAGCCGACCAAGCACCAGTATGACGTCGACACGGAGACCGTCATCGGTTTCCTCCGCCAGAATGGCCTCGACAAGGACTTCAAGGTCAATATCGAGGTGAACCACGCCACCCTCGCCGGCCATACCTTCGAGCACGAGCTGACCGCGGCCCGGGAGAACGGCTTCCTCGGCAGCATCGACGCCAACCGCGGCGACGCCCAGAACGGCTGGGACACCGACCAGTTCCCGGTGGACGCCTTCGATCTCACGCGGGCCATGATGCAGATCCTGCTCAATGGCGGTTTCGGCAACGGCGGCACCAACTTCGACGCCAAGCTGCGCCGCAGCTCCACCGATCCCGAGGACATCTTCATCGCCCACATCAGCGCGATGGACGCCATGGCCCACGCCCTGCTGAATGCGGCCGCCATCCTCGAGGAAAGCCCGCTGCCGGCCCTGGTCAAGCAGCGCTATGCGTCCTTCGACAGCGGTCTCGGCAAGCAGTTCGAGGAGGGTAAGGCCACGCTCGAGGACCTGTACGCATACGCGAAGGAGCACGGCGAGCCCGTCGCGGCCTCCGGCAAGCAGGAGCTCTGCGAGACCTATCTCAACCTCTACGCGAAATAA 5586MI215_003Prevotella Amino 126MAKEYFPQIGKIGFEGLESKNPMAFHYYDAERVVLGKKMKDWLKFAMANWHTLGQASGDP AcidFGGQTRSYENDKGECPYCRARAKADAGFELMQKLGIEYFCFHDIDLIEDCDDIDEYEARMKDITDYLLEKMKETGIKNLWGTANVFGHKRYMNGAATNPQFEIVARAAVQIKNALDATIKLGGSNYVFWGGREGYYTLLNTQMQREKDHLARLLTAARDYARAKGFKGTFPIEPKPMEPTKHQYDVDTETVIGFLRQNGLDKDEKVNIEVNHATLAGHTFEHELTAARENGFLGSIDANRGDAQNGWDTDQFPVDAFDLTRAMMQILLNGGFGNGGTNFDAKLRRSSTDPEDIFIAHISAMDAMAHALLNAAAILEESPLPALVKQRYASFDSGLGKQFEEGKATLEDLYAYAKEHGEPVAASGKQELCETYLNLYAK 5607MI1_003 Prevotella DNA 127ATGAGTAAAGAGTATTTTCCTGGGATTGGCAAAATCCCGTATGAGGGAGCCGAGAGCAAGAATGTGATGGCATTCCACTATTATGATCCCGAACGCGTGGTCATGGGCAAGAAAATGAAAGACTGGTTCAAGTTCGCTATTGCCTGGTGGCATACCCTGGGGCAGGCCAGTGCTGACCAGTTTGGCGGACAGACCCGTTTCTATGAATGGGACAAAGCCGAGGACCCCTTGCAGCGTGCCAAGGACAAGATGGATGCCGGTTTTGAAATCATGCAGAAGCTGGGCATCGAGTATTTCTGTTTCCATGATGTGGACCTCATCGAGGAGGCCGATACCATCGAGGAATATGAAGCCCGCATGCAGGCGATTACCGACTACGCGCTGGAGAAGATGAAGGCAACGGGTATCAAGTTGCTGTGGGGCACTGCCAACGTGTTCGGCCACAAGCGTTACATGAACGGCGCCGCCACCAATCCCGACTTCAATGTCGTGGCACGTGCAGCCGTGCAGATCAAGAACGCCCTCGATGCTACCATCAAGTTGGGCGGAACGAGCTACGTCTTCTGGGGCGGTCGTGAAGGCTATCAGAGCCTGCTCAACACCCAGATGCAGCGCGAGAAGAACCACCTGGCCAAGATGCTCACGGCAGCCCGTGACTATGCCCGTGCTAAGGGCTTCAAGGGCACCTTCCTGATTGAGCCCAAGCCGATGGAACCCACCAAGCACCAGTATGACCAGGACACCGAGACCGTTATCGGCTTCTTGCGTGCCAATGGCCTTGACAAGGACTTTAAGGTCAACATTGAGGTCAACCATGCCACGCTGGCTGGCCACACCTTTGCACATGAGTTGGCAGTGGCTGTGGATAACGGTATGCTGGGCAGCATCGATGCTAACCGTGGTGACCACCAGAACGGCTGGGATACAGACCAGTTCCCCATCAACAGTTATGAACTCACCAATGCTATGCTGCAGATCATGCACGGCGGCGGTTTCAAGGACGGCGGTACCAACTTTGACGCCAAGCTGCGCCGCAACAGTACCGACCCCGAGGACATCTTTACCGCTCACATCAGTGGTATGGACGCTCTGGCCCGTGCCCTGTTGAGTGCTGCCGATATCCTTGAGAAGAGCGAGTTGCCTGAAATGCTCAAGGAACGCTATGCCAGCTTTGACGCGGGTGAAGGCAAGCGCTTTGAGGATGGCCAGATGACTCTTGAGGAACTGGTTGCCTATGCCAAGTCCCATGGCGAGCCTGCTACCATCAGTGGCAAGCAGGAAAAATATGAAGCCATCGTGGCTTTGCACGTCAAGTAA 5607MI1_003Prevotella Amino 128MSKEYFPGIGKIPYEGAESKNVMAFHYYDPERVVMGKKMKDWFKFAIAWWHTLGQASADQ AcidFGGQTREYEWDKAEDPLQRAKDKMDAGFEIMQKLGIEYFCFHDVDLIEEADTIEEYEARMQAITDYALEKMKATGIKLLWGTANVFGHKRYMNGAATNPDFNVVARAAVQIKNALDATIKLGGTSYVFWGGREGYQSLLNTQMQREKNHLAKMLTAARDYARAKGFKGTFLIEPKPMEPTKHQYDQDTETVIGFLRANGLDKDEKVNIEVNHATLAGHTFAHELAVAVDNGMLGSIDANRGDHQNGWDTDQFPINSYELTNAMLQIMHGGGFKDGGTNFDAKLRRNSTDPEDIFTAHISGMDALARALLSAADILEKSELPEMLKERYASFDAGEGKRFEDGQMTLEELVAYAKSHGEPATISGKQEKYEAIVALHVK 5607MI2_003 Prevotella DNA 129ATGAGTAAAGAGTATTATCCTGAGATTGGCAAAATCCCGTTTGAGGGTCCCGAGAGCAAGAATGTGATGGCGTTCCATTACTATGAACCCGAACGCGTCGTCATGGGTAAGAAGATGAAAGACTGGCTCAAGTTTGCCATGTGCTGGTGGCACAGCCTGGGTCAGGCCAGTGCCGACCAGTTCGGCGGACAGACACGTTTCTACGAGTGGGACAAGGCCGATACCCCCCTGCAGCGTGCCAAGGACAAAATGGATGCCGGATTTGAAATCATGCAGAAGTTGGGCATCGAGTACTTCTGCTTCCACGATGTGGACCTCATCGAGGAGGCCGATACCATCGAGGAATACGAGGCCCGCATGAAGGCCATTACCGACTATGCGCTGGAGAAGATGCAGGCCACCGGCATCAAGTTGCTGTGGGGCACTGCCAATGTGTTCGGCCACAAGCGCTACATGAACGGCGCCGCCACCAATCCCGATTTCAATGTCGTGGCACGTGCCGCCGTCCAAATCAAGAATGCCATCGATGCCACCATCAAGCTGGGCGGCACGAGTTACGTCTTCTGGGGTGGTCGTGAGGGCTATCAGAGTCTGCTCAACACGCAGATGCAGCGCGAGAAGGACCATCTGGCCCGCATGCTGGCGGCAGCCCGCGACTATGGCCGTGCCCATGGCTTCAAGGGCACTTTCCTGATCGAGCCCAAACCCATGGAGCCCACCAAGCACCAGTATGATGTGGACACCGAGACCGTGCTCGGCTTCCTGCGTGCCCACGGCCTGGACAAGGACTTCAAGGTTAACATCGAGGTCAATCATGCTACGCTGGCGGGACACACTTTCAGCCACGAACTGGCTGTGGCCGTGGACAACGGTATGCTGGGCAGCATCGACGCCAACCGCGGCGATTATCAGAATGGCTGGGACACCGACCAGTTCCCCATCGACAGCTTCGAGCTCACCCAGGCCATGCTGCAGATCATGCGCGGCGGCGGCTTCAAGGACGGAGGTACCAACTTCGATGCCAAGCTGCGTCGCAACAGTACCGACCCTGAGGACATCTTCATCGCCCACATCAGCGGTATGGATGCCATGGCACGCGGCCTGTTGAGCGCTGCCGCTATCCTCGAGGATGGCGAGTTGCCCGCGATGCTCAAGGCACGTTATGCCAGCTTTGACCAGGGCGAGGGTAAGCGCTTTGAGGACGGCGAGATGACGCTCGAGCAGCTGGTGGATTATGCAAAGGATTATGCCAAATCGCACGGCGAGCCTGATGTCATCAGCGGCAAGCAGGAGAAGTTTGAAACCATCGTGGCCCTTTAC GCCAAGTAA5607MI2_003 Prevotella Amino 130MSKEYYPEIGKIPFEGPESKNVMAFHYYEPERVVMGKKMKDWLKFAMCWWHSLGQASADQ AcidFGGQTRFYEWDKADTPLQRAKDKMDAGFEIMQKLGIEYFCFHDVDLIEEADTIEEYEARMKAITDYALEKMQATGIKLLWGTANVFGHKRYMNGAATNPDFNVVARAAVQIKNAIDATIKLGGTSYVFWGGREGYQSLLNTQMQREKDHLARMLAAARDYGRAHGFKGTFLIEPKPMEPTKHQYDVDTETVLGFLRAHGLDKDFKVNIEVNHATLAGHTFSHELAVAVDNGMLGSIDANRGDYQNGWDTDQFPIDSFELTQAMLQIMRGGGFKDGGTNFDAKLRRNSTDPEDIFIAHISGMDAMARGLLSAAAILEDGELPAMLKARYASFDQGEGKRFEDGEMTLEQLVDYAKDYAKSHGEPDVISGKQEKFETIVALYAK 5607M13_003 Prevotella DNA 131ATGACCAACGAGTATTTTCCCGGAATCGGTGTGATTCCGTTTGAAGGACAGGAAAGCAAGAATCCCCTGGCTTTCCATTATTATGACGCCAACCGCGTAGTGATGGGCAAACCCATGAAGGAATGGTTCAAATTTGCCATGGCCTGGTGGCATACGCTGGGGCAGGCATCGGCCGATCCCTTCGGCGGACAGACCCGCTCCTACGCATGGGACAAGGGCGAGTGCCCTTACTGCCGTGCCCGCCAGAAGGCCGACGCCGGCTTTGAACTGATGCAGAAGCTGGGAATCGGCTATTTCTGCTTCCACGATGTGGATATCATCGAGGACTGCGAGGACATTGCCGAGTATGAGGCCCGTATGAAGGACATCACGGACTATCTGCTGGTGAAGATGAAGGAAACGGGCATCAAGAATCTGTGGGGCACGGCCAACGTCTTCGGCCACAAGCGCTATATGAACGGCGCCGCCACCAACCCGCAATTCGACGTGGTAGCCCGCGCTGCGGTCCAGATCAAGAACGCCCTGGACGCCACCATCAAGCTGGGCGGCAGCAATTATGTGTTCTGGGGCGGCCGGGAAGGCTACTACACCCTTTTGAACACGCAGATGCAGCGGGAGAAGGACCACCTGGCCCAGATGCTCAAGGCGGCCCGCGACTATGCCCGCGGCAAGGGATTCAAGGGCACGTTCCTCATTGAGCCCAAGCCCATGGAGCCCACCAAGCACCAGTACGACGTAGATACGGAGACCGTGATTGGTTTCCTGCGCGCCAACGGGCCGGACAAGGACTTCAAGGTGAATATCGAAGTGAACCACGCCACCCTGGCCGGCCATACCTTCGAGCACGAGCTCACCGTGGCCCGCGAAAACGGCTTCCTGGGCAGCATCGACGCCAACCGCGGAGACGCCCAGAACGGCTGGGATACAGACCAGTTCCCCGTGGACGCCTTTGACCTCACCCAGGCCATGATGCAGGTCCTGCTCAACGGCGGATTCGGCAACGGCGGCACCAACTTCGACGCCAAACTGCGCCGTTCCTCCACGGATCCCGAGGACATCTTCATCGCCCACATCAGCGCCATGGACGCCATGGCCCACGCCCTCCTGAACGCCGCCGCCATCCTGGAAGAGAGCCCCATGCCGGGCATGGTGAAGGAGCGCTACGCTTCCTTCGACAATGGCCTTGGCAAGAAGTTCGAGGAAGGAAAGGCCACGCTGGAAGAGCTGTACGACTATGCCAAGAAGAACGGCGAGCCTGTGGCCGCTTCCGGAAAGCAGGAACTGTACGAAACGCTGCTGAACCTGTACGCCAAGTAA 5607M13_003Prevotella Amino 132MTNEYFPGIGVIPFEGQESKNPLAFHYYDANRVVMGKPMKEWFKFAMAWWHTLGQASADP AcidFGGQTRSYAWDKGECPYCRARQKADAGFELMQKLGIGYFCFHDVDIIEDCEDIAEYEARMKDITDYLLVKMKETGIKNLWGTANVEGHKRYMNGAATNPQEDVVARAAVQIKNALDATIKLGGSNYVFWGGREGYYTLLNTQMQREKDHLAQMLKAARDYARGKGFKGTFLIEPKPMEPTKHQYDVDTETVIGFLRANGPDKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANRGDAQNGWDTDQFPVDAFDLTQAMMQVLLNGGFGNGGTNFDAKLRRSSTDPEDIFIAHISAMDAMAHALLNAAAILEESPMPGMVKERYASFDNGLGKKFEEGKATLEELYDYAKKNGEPVAASGKQELYETLLNLYAK 5607M14_005 Prevotella DNA 133ATGACTAAAGAGTATTTCCCTTCCGTCGGCAAGATTGCCTTTGAAGGACCCGAAAGCAAGAACCCTATGGCCTTCCATTATTATGACGCCAATCGCGTGGTAATGGGAAAGCCGATGAAAGAATGGCTTAAATTTGCCATGGCCTGGTGGCACACCCTGGGCCAGGCCTCTGCAGACCCCTTCGGCGGTCAGACCCGCTCCTACGAGTGGGACAAGGGCGAGTGCCCCTACTGCCGCGCCAAGGCCAAGGCCGATGCCGGCTTTGAACTGATGCAGAAACTGGGCATCGAGTATTTCTGCTTCCACGATATAGACCTGGTGGAAGACTGCGATGATATCGCCGAATACGAGGCCCGCATGAAGGACATCACGGACTATCTCCTGGAGAAGATGAAGGAAACCGGCATCAAGAACCTCTGGGGAACCGCCAACGTGTTCGGCCACAAGCGCTATATGAACGGCGCCGCCACCAACCCTCAGTTCGACATCGTGGCCCGTGCCGCTGTCCAGATCAAGAACGCCCTGGATGCCACCATCAAGCTGGGCGGCTCCAACTATGTGTTCTGGGGCGGCCGTGAGGGCTACTATACCCTCCTGAACACCCAGATGCAGAGAGAGAAGGACCACCTGGCCAAGATGCTCACCGCCGCCCGCGACTATGCCCGTGCCAAGGGCTTCAAGGGCACCTTCCTCATCGAACCCAAGCCGATGGAGCCCACCAAGCACCAGTACGACGTAGATACGGAGACCGTGATCGGCTTCCTCCGCGCCAACGGCCTGGACAAGGACTTCAAGGTGAATATTGAGGTGAACCACGCCACCCTGGCCGGCCACACCTTCGAGCACGAGCTCACCGTGGCCCGCGAGAACGGCTTCCTGGGCAGCATCGACGCCAACCGCGGAGACGCCCAGAACGGCTGGGATACGGACCAGTTCCCGGTGGATGCCTTCGACCTCACCCAGGCTATGATGCAGATCCTTCTGAACGGAGGCTTCGGCAACGGCGGTACCAACTTCGACGCCAAACTGCGCCGCTCCTCCACGGACCCCGAGGACATCTTCATCGCCCACATCAGCGCTATGGATGCCATGGCCCACGCCCTGCTGAATGCAGCCGCCATCCTGGAGGAAAGCCCGCTTCCGAAGATGCTGAAAGAGCGTTATGCCAGCTTTGACGGCGGTCTGGGCAAGAAGTTCGAAGAAGGCAAGGCCTCTCTGGAAGAACTCTACGAGTATGCCAAGAGCAACGGAGAGCCCGTGGCCGCTTCCGGCAAGCAGGAGCTCTGCGAAACGTACCTGAACCTCTACGCTAAGTAA 5607M14_005Prevotella Amino 134MTKEYFPSVGKIAFEGPESKNPMAFHYYDANRVVMGKPMKEWLKFAMAWWHTLGQASADP AcidFGGQTRSYEWDKGECPYCRAKAKADAGFELMQKLGIEYFCFHDIDLVEDCDDIAEYEARMKDITDYLLEKMKETGIKNLWGTANVFGHKRYMNGAATNPQFDIVARAAVQIKNALDATIKLGGSNYVFWGGREGYYTLLNTQMQREKDHLAKMLTAARDYARAKGFKGTFLIEPKPMEPTKHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANRGDAQNGWDTDQFPVDAFDLTQAMMQILLNGGFGNGGTNFDAKLRRSSTDPEDIFIAHISAMDAMAHALLNAAAILEESPLPKMLKERYASFDGGLGKKFEEGKASLEELYEYAKSNGEPVAASGKQELCETYLNLYAK 5607M15_002 Prevotella DNA 135ATGGCTAAAGAATACTTCCCCTCCATCGGCAAAATCCCTTTTGAAGGAGCCGACAGCAAAAATCCCCTCGCTTTCCATTATTATGACGCCGGACGCGTGGTTATGGGCAAGCCCATGAAGGAATGGCTTAAATTCGCCATGGCCTGGTGGCACACGCTGGGCCAGGCCTCCGGAGACCCCTTCGGCGGCCAGACCCGCAGCTACGAATGGGACAAGGGCGAATGCCCCTACTGCCGCGCCAAGGCCAAGGCCGACGCCGGTTTTGAAATCATGCAAAAGCTGGGCATCGAATACTTCTGCTTCCACGATGTGGACCTTATCGAGGATTGCGATGACATTGCCGAATACGAAGCCCGCATGAAGGACATCACGGACTACCTGCTGGAAAAGATGAAGGAGACCGGCATCAAGAACCTCTGGGGCACCGCCAATGTCTTCGGCCACAAGCGCTACATGAACGGCGCCGGCACCAATCCGCAGTTCGATGTGGTGGCCCGTGCCGCCGTCCAGATCAAGAACGCCCTGGACGCCACCATCAAGCTGGGCGGCTCCAACTATGTGTTCTGGGGCGGCCGCGAAGGCTATTACACCCTCCTCAACACACAGATGCAGCGGGAAAAAGACCACCTGGCCAAGTTGCTGACGGCCGCCCGCGACTATGCCCGCGCCAAGGGCTTCAAGGGCACCTTCCTCATTGAGCCCAAACCCATGGAACCCACCAAGCACCAGTACGACGTGGATACGGAGACGGTCATCGGCTTCCTCCGTGCCAACGGCCTGGACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCCACCCTGGCCGGCCACACCTTCGAGCATGAGCTCACCGTGGCCCGCGAGAACGGTTTCCTGGGCTCCATCGATGCCAACCGCGGCGACGCCCAGAACGGCTGGGACACGGACCAGTTCCCTGTGGACCCGTACGATCTTACCCAGGCCATGATGCAGGTGCTGCTGAACGGCGGCTTCGGCAACGGCGGCACCAACTTCGACGCCAAACTCCGCCGCTCCTCCACCGACCCTGAGGACATCTTCATCGCCCATATTTCCGCCATGGATGCCATGGCCCACGCTTTGCTTAACGCAGCTGCCGTGCTGGAAGAGAGCCCCCTGTGCCAGATGGTCAAGGAGCGTTATGCCAGCTTCGACGATGGCCTCGGCAAACAGTTCGAGGAAGGCAAGGCTACCCTGGAAGACCTGTACGAATACGCCAAGGCCCAGGGTGAACCCGTTGTCGCCTCCGGCAAGCAGGAGCTTTACGAGACTCTCCTGAACCTGTATGCCGTCAAGTAA 5607M15_002Prevotella Amino 136MAKEYFPSIGKIPFEGADSKNPLAFHYYDAGRVVMGKPMKEWLKFAMAWWHTLGQASGDP AcidFGGQTRSYEWDKGECPYCRAKAKADAGFEIMQKLGIEYFCFHDVDLIEDCDDIAEYEARMKDITDYLLEKMKETGIKNLWGTANVFGHKRYMNGAGTNPQFDVVARAAVQIKNALDATIKLGGSNYVFWGGREGYYTLLNTQMQREKDHLAKLLTAARDYARAKGFKGTFLIEPKPMEPTKHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANRGDAQNGWDTDQFPVDPYDLTQAMMQVLLNGGFGNGGTNFDAKLRRSSTDPEDIFIAHISAMDAMAHALLNAAAVLEESPLCQMVKERYASFDDGLGKQFEEGKATLEDLYEYAKAQGEPVVASGKQELYETLLNLYAVK 5607M16_002 Prevotella DNA 137ATGACCAAAGAATATTTCCCTACCGTCGGGAAGATCCCCTTCGAGGGCCCCGAAAGCAAGAACCCTATGGCGTTCCATTACTATGACCCCAACCGTCTGGTGATGGGCAAGAAGATGAAAGACTGGCTGCGTTTCGCCATGGCCTGGTGGCACACCCTCGGCCAGGCGTCGGGCGACCAGTTCGGCGGCCAGACCCGCAGTTATGCGTGGGACGAGGGAGAATGCCCGTACGAGCGCGCCCGTGCCAAGGCTGACGCCGGCTTCGAGATCATGCAGAAACTCGGTATCGAGTTCTTCTGCTTCCACGACATCGACCTGATCGAGGATACCGACGACATCGCCGAGTATGAGGCCCGCCTGAAAGACATCACGGACTATCTGCTCGAGAAGATGAAAGCCACTGGCATCAAAAATCTCTGGGGAACGGCCAACGTGTTCGGCCACAAGCGTTGCATGAACGGCGCCGCCACCAACCCGGACTTCGCCGTGCTGGCCCGCGCTGCCGTCCAGATCAAGAACGCCATCGACGCCACCATCAAGCTGGGCGGCGAGAACTATGTGTTCTGGGGTGGCCGCGAAGGCTACACGAGCCTGCTCAACACCCAGATGCAGCGTGAGAAAGAGCACCTGGGCCGCCTGCTGTCCCTGGCCCGCGACTATGGCCGCGCCCACGGCTTCAAGGGTACCTTCCTGATCGAGCCCAAGCCGATGGGACCGACGAAACACCAGTACGACCAGGATACGGAAACTGTCATCGGTTTCCTGCGCCGCCACGGTCTAGACAAGGACTTCAAGGTCAATATCGAGGTGAACCATGCCACGCTGGCGGGCCACACCTTCGAACACGAACTGGCCTGCGCCGTGGATCACGGTATGCTGGGCAGCATCGACGCCAACCGCGGTGACGCACAGAACGGCTGGGATACCGACCAGTTCCCGATCGACAACTTCGAGCTGACCCTTTCCATGCTCCAGATCATCCGCAACGGTGGCCTGGCACCCGGCGGCTCGAATTTCGATGCCAAGCTGCGCCGCAACTCCACCGATCCCGAAGACATTTTCATCGCGCACATCAGCGCCATGGACGCCATGGCCCGCGCATTGGTCAATGCGGCCGCCATCCTGGAGGAGAGCGCTATTCCGAAGATGGTCAAGGAGCGTTACGCTTCGTTCGACAGCGGCAAAGGCAAGGAATACGAGGAAGGCAAGCTGACGCTCGAAGACATCGTGGCCTATGCCAAGGCGAACGGAGAACCGAAGCAGATTTCCGGCAAACAGGAACTCTACGAGACGCTTGTCGCACTCTATAGCAAATAA 5607M16_002Prevotella Amino 138MTKEYFPTVGKIPFEGPESKNPMAFHYYDPNRLVMGKKMKDWLRFAMAWWHTLGQASGDQ AcidFGGQTRSYAWDEGECPYERARAKADAGFEIMQKLGIEFFCFHDIDLIEDTDDIAEYEARLKDITDYLLEKMKATGIKNLWGTANVFGHKRCMNGAATNPDFAVLARAAVQIKNAIDATIKLGGENYVFWGGREGYTSLLNTQMQREKEHLGRLLSLARDYGRAHGFKGTFLIEPKPMGPTKHQYDQDTETVIGFLRRHGLDKDFKVNIEVNHATLAGHTFEHELACAVDHGMLGSIDANRGDAQNGWDTDQFPIDNFELTLSMLQIIRNGGLAPGGSNFDAKLRRNSTDPEDIFIAHISAMDAMARALVNAAAILEESAIPKMVKERYASFDSGKGKEYEEGKLTLEDIVAYAKANGEPKQISGKQELYETLVALYSK 5607M17_002 Prevotella DNA 139ATGACCAAAGGGTATTTCCCTACCATCGGCAGGATTCCCTTCGAGGGAACTGAAAGCAAGAATCCCCTCGCATTCCATTACTATGAGCCCGACCGGCTCGTACTGGGCAAGAAAATGAAAGACTGGCTGCGTTTCGCGATGGCCTGGTGGCACACCCTGGGCCAGGCGTCCGGCGACCAGTTCGGCGGCCAGACCCGCAGCTATGCCTGGGACAAGGCCGAGTGCCCCTATGAGCGCGCCAAGGCCAAAGCCGACGCCGGCTTCGAGATCATGCAGAAACTCGGCATCGAGTTCTTCTGTTTCCACGACATTGACCTCGTTGAGGATACCGACGACATCGCCGAGTATGAGGCCCGGATGAAGGACATTACCGACTATCTCCTGGTCAAGATGAAGGAGACCGGAATCAAGAACCTCTGGGGTACGGCCAATGTCTTCGGCCACAAGCGCTATATGAACGGCGCCGCCACCAATCCCGACTTCGACGTGGTGGCCCGCGCCGCCGTCCAGATCAAGAACGCCCTCGATGCCACCATCAAGCTGGGCGGTGAAAACTATGTGTTCTGGGGCGGCCGCGAAGGCTATATGAGCCTGCTCAACACGCAGATGCAGCGTGAGAAGGAGCACCTGGGCCGGATGCTGGTCGCCGCCCGCGACTACGCCCGCGCCCACGGCTTCAAGGGTACCTTCCTCATCGAGCCCAAACCGATGGAACCGACCAAGCACCAGTACGACCAGGATACGGAAACCGTGATCGGCTTCCTTCGCCGCCACGGCCTGGACAAGGATTTCAAGGTGAACATCGAAGTGAACCACGCCACGCTGGCCGGCCACACCTTCGAGCACGAACTGGCCACCGCCGTCGACTGCGGCCTGCTGGGCAGCATCGACGCCAATCGCGGCGACGCTCAGAACGGCTGGGATACCGACCAGTTCCCGATCGACAACTTCGAACTCACGCTGGCCATGCTGCAGATTATCCGCAACGGCGGTCTGGCACCCGGCGGCTCGAACTTCGACGCCAAACTGCGCCGTAACTCCACCGATCCGGAAGATATCTTCATCGCCCACATCAGTGCGATGGACGCGATGGCCCGTGCGCTGGTCAACGCCGCCGCAATCTGGGAAGAGTCTCCCATCCCGCAGATGAAGAAAGAACGCTACGCGTCGTTCGACAGCGGCAAGGGCAAGGAATTCGAAGAGGGCAAGCTCTGCCTCGAAGACCTCGTGGCCTATGCCAAGGCGAACGGAGAACCGAAACAGATCTCCGGCAGGCAGGAACTATATGAGACCATCGTCGCCCTTTATTGCAAATAG 5607M17_002Prevotella Amino 140MTKGYFPTIGRIPFEGTESKNPLAFHYYEPDRLVLGKKMKDWLRFAMAWWHTLGQASGDQ AcidFGGQTRSYAWDKAECPYERAKAKADAGFEIMQKLGIEFFCFHDIDLVEDTDDIAEYEARMKDITDYLLVKMKETGIKNLWGTANVFGHKRYMNGAATNPDFDVVARAAVQIKNALDATIKLGGENYVFWGGREGYMSLLNTQMQREKEHLGRMLVAARDYARAHGFKGTFLIEPKPMEPTKHQYDQDTETVIGFLRRHGLDKDFKVNIEVNHATLAGHTFERELATAVDCGLLGSIDANRGDAQNGWDTDQFPIDNFELTLAMLQIIRNGGLAPGGSNFDAKLRRNSTDPEDIFIAHISAMDAMARALVNAAAIWEESPIPQMKKERYASFDSGKGKEFEEGKLCLEDLVAYAKANGEPKQISGRQELYETIVALYCK 5608MI1_004 Prevotella DNA 141ATGACCAACGAGTATTTTCCCGGAATCGGTGTGATTCCGTTTGAAGGACAGGAAAGCAAGAATCCCATGGCTTTCCATTATTATGACGCCAACCGCGTAGTGATGGGCAAACCCATGAAGGAATGGTTCAAATTTGCCATGGCCTGGTGGCATACGCTGGGGCAGGCATCGGCCGATCCCTTCGGCGGACAGACCCGCTCCTACGCATGGGACAAGGGCGAGTGCCCTTACTGCCGTGCCCGCCAGAAGGCCGACGCCGGCTTTGAACTGATGCAGAAGCTGGGTATCGGCTATTTCTGCTTCCACGATGTGGATATCATCGAGGACTGCGAAGACATTGCCGAGTATGAGGCCCGTATGAAGGACATCACGGACTATCTGCTGGTGAAGATGAAGGAAACGGGCATCAAGAACCTGTGGGGCACGGCCAACGTCTTCGGCCACAAGCGCTATATGAACGGCGCTGCCACCAACCCGCAGTTCGACGTGGTGGCCCGCGCTGCGGTCCAGATCAAGAACGCCCTGGACGCCACCATCAAGCTGGGCGGCAGCAATTACGTGTTCTGGGGCGGCCGCGAAGGCTATTATACCCTTTGGAACACGCAGATGCGGCGGGAGAAGGACCACCTGGCCCAGATGCTCAAGGCAGCCCGTGACTATGCCCGCGGCAAGGGATTCAAGGGCACGTTCCTCATTGAGCCCAAGCCCATGGAGCCCACCAAGCACCAGTACGACGTAGATACGGAGACCGTGATTGGCTTCCTGCGCGCAAACGGACTGGACAAGGACTTCAAGGTGAATATCGAAGTGAACCACGCCACCCTGGCCGGCCACACCTTCGAGCACGAACTCACCGTGGCCCGCGAAAACGGCTTCCTGGGCAGCATCGACGCCAACCGCGGAGACGCCCAGAACGGTTGGGATACAGACCAGTTCCCCATAGATGCCTTTGACCTCACCCAGGCCATGATGCAGGTCCTGCTCAACGGCGGATTCGGCAACGGCGGCACCAACTTCGACGCCAAACTGCGCCGTTCCTCCACGGATCCCGAGGACATCTTCATCGCCCACATCGGCGCCATGGACGCCATGGCCCACGCCCTCCTGAACGCCGCCGCCATCCTGGAAGAGAGCCCCATGCCGGGCATGGTGAAGGAGCGCTACGCTTCCTTCGACAATGGCCTTGGCAAGAAGTTCGAGGAAGGAAAGGCCACGCTGGAAGAGCTGTACGACTATGCCAAGAAGAACGGCGAGCCTGTGGCCGCTTCCGGCAAGCAGGAACTGTACGAAACGCTGCTGAACCTGTACGCCAAGTAA 5608MI1_004Prevotella Amino 142MTNEYFPGIGVIPFEGQESKNPMAFHYYDANRVVMGKPMKEWFKFAMAWWHTLGQASADP AcidFGGQTRSYAWDKGECPYCRARQKADAGFELMQKLGIGYFCFHDVDIIEDCEDIAEYEARMKDITDYLLVKMKETGIKNLWGTANVFGHKRYMNGAATNPQFDVVARAAVQIKNALDATIKLGGSNYVFWGGREGYYTLWNTQMRREKDHLAQMLKAARDYARGKGFKGTFLIEPKPMEPTKHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANRGDAQNGWDTDQFPIDAFDLTQAMMQVLLNGGFGNGGTNFDAKLRRSSTDPEDIFIAHIGAMDAMAHALLNAAAILEESPMPGMVKERYASFDNGLGKKFEEGKATLEELYDYAKKNGEPVAASGKQELYETLLNLYAK 5608MI2_002 Prevotella DNA 143ATGAAAGAATACTTCCCTACCATCGGAAAAATCCCTTTCGAGGGCCCTCAGAGCAAGAATCCGCTCGCATTCCATTACTATGACGCCAACCGCGTTGTCGCCGGCAAACCCATGAAGGACTGGCTCAAGTTCGCCATGGCTTGGTGGCACACCCTGGGCGCAGCATCGGCAGACCCCTTCGGCGGCCAGACCCGCAGCTACGAGTGGGACAAAGCCGAGTGCCCTTACTGCCGTGCCCGTGAAAAGGCCGACGCCGGCTTCGAGATCATGCAGAAACTTGGAATCGAGTACTTCTGCTTCCATGACATCGACCTTGTGGAAGACTGCGAGGACATTGCCGAGTACGAGGCCCGCATGAAGGACATCACGGACTACCTCCTGGAGAAGATGAAGGCCACCGGCATCAAGAACCTGTGGGGCACCGCCAACGTCTTTGGCAACAAGCGCTACATGAACGGCGCAGCCACCAACCCTCAGTTCGACATCGTTGCCCGTGCAGCTGTCCAGATCAAGAACGCCATCGACGCAACAATCAAGCTGGGCGGTACCGGTTACGTATTCTGGGGCGGCCGCGAGGGCTACTACACCCTCCTGAACACCCAGATGCAGCGCGAGAAGGACCACCTTGCCAAGATGCTCACCGCAGCCCGCGACTACGCCCGCGCCAAGGGATTCAAGGGCACATTCCTCATCGAGCCCAAGCCCATGGAGCCCACCAAGCACCAGTACGATGTTGACACGGAAACCGTCATCGGCTTCCTCCGCGCCAACGGCCTGGACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCCACCCTGGCCGGCCACACCTTCGAGCACGAGCTCACCGTGGCCGTGGACAACGGCTTCCTGGGCAGCATCGACGCAAACCGCGGCGACGCCCAGAACGGCTGGGACACTGACCAGTTCCCTGTGGATCCTTACGACCTCACCCAGGCAATGATGCAGATTATCCGCAACGGCGGCTTCAAGGACGGCGGCACCAACTTCGACGCCAAACTCCGCCGCAGCTCCACGGACCCCGAGGACATCTTCATCGCCCACATCAGCGCAATGGATGCAATGGCACACGCCCTCATCAACGCTGCTGCAGTGCTTGAGGAAAGCCCTCTGTGCGAGATGGTTGCAAAGCGCTACGCCAGCTTTGACAGCGGTCTTGGCAAGAAGTTCGAGGAAGGCAAAGCCACTCTCGAGGAGATCTACGAGTATGCCAAGAAGGCCCCGGCACCCGTCGCCGCCTCCGGCAAGCAGGAGCTCTACGAGACACTGCTCAATCTGTACGCTAAATAA 5608MI2_002Prevotella Amino 144MKEYFPTIGKIPFEGPQSKNPLAFHYYDANRVVAGKPMKDWLKFAMAWWHTLGAASADPF AcidGGQTRSYEWDKAECPYCRAREKADAGFEIMQKLGIEYFCFHDIDLVEDCEDIAEYEARMKDITDYLLEKMKATGIKNLWGTANVFGNKRYMNGAATNPQFDIVARAAVQIKNAIDATIKLGGTGYVFWGGREGYYTLLNTQMQREKDHLAKMLTAARDYARAKGFKGTFLIEPKPMEPTKHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVAVDNGFLGSIDANRGDAQNGWDTDQFPVDPYDLTQAMMQIIRNGGFKDGGTNFDAKLRRSSTDPEDIFIAHISAMDAMAHALINAAAVLEESPLCEMVAKRYASFDSGLGKKFEEGKATLEEIYEYAKKAPAPVAASGKQELYETLLNLYAK 5608MI3_004 Prevotella DNA 145ATGACCAAAGAGTATTTCCCTACAATCGGAAAGATTCCCTTCGAAGGCCCGGAGAGCAAGAATCCGCTGGCATTCCATTACTATGAACCCGACAGAATCATCCTCGGCAGGAAGATGAAGGACTGGCTGCGCTTCGCCGTGGCCTGGTGGCACACCCTCGGCCAGGCGTCCGGCGACCAGTTCGGAGGCCAGACCCGCAACTATGCGTGGGACGAGCCCGAATGCCCGGTAGAGCGCGCGAAAGCCAAGGCCGACGCCGGCTTCGAGCTGATGCAGAAGCTGGGCATCGAGTATTTCTGCTTCCACGACGTAGACCTCATAGAGGAGGCCGCAACCATCGAAGAATATGAGGAGCGCATGGGCATCATAACCGACTACCTGCTCGGGAAGATGAAGGAGACAGGTATCAAGAACCTCTGGGGCACCGCCAACGTGTTCGGCCACAAGCGTTACATGAACGGAGCCGCCACCAACCCCGACTTCGACGTGGTGGCCCGTGCGGCCGTGCAGATCAAGAACGCCATCGACGCCACCATCAAGCTGGGCGGCGAGAATTACGTATTCTGGGGCGGACGCGAGGGCTATGCAAGCCTGCTCAACACTCAGATGCAGCGCGAGAAAGACCACCTGGGACGCATGCTGGCTGCAGCCCGCGACTATGGCCGCGCCCACGGATTCAAGGGCACTTTCCTCATCGAGCCCAAACCCATGGAGCCTACCAAGCACCAGTACGACCAGGATACCGAGACCGTTATCGCCTTCCTGCGCAGGAACGGCCTCGACAAGGATTTCAAGGTAAACATCGAGGTGAACCACGCCACCCTGGCGGGCCACACCTTCGAGCACGAACTGGCGGTGGCAGTGGACAACGGCCTGCTTGGCAGCATCGACGCCAACCGCGGCGACGCGCAGAACGGATGGGACACCGACCAGTTCCCCATCGACAACTTCGAGCTCACCCAGGCCATGCTGCAGATAATCCGCAACGGCGGACTGGGAACCGGCGGATCGAACTTCGACGCCAAGCTGCGCCGCAATTCCACCGACCCTGAGGATATCTTCATCGCCCACATCAGTGCGATGGACGCCATGGCACGCGCGCTGGCAAACGCCGCCGCAATCATCGAAGAGAGCCCCATCCCCGCAATGCTGAAGGAGCGCTACGCATCGTTCGACAGCGGCAAGGGCAAGGAGTTCGAGGACGGCAAACTGAGCCTCGAAGAACTGGTAGCCTACGCCAAGGCGAACGGCGAGCCGAAGCAGATTTCCGGCAAGCAGGAACTCTACGAAACCATAGTGGCCCTCTATTGCAAGTAA 5608MI3_004Prevotella Amino 146MTKEYFPTIGKIPFEGPESKNPLAFHYYEPDRIILGRKMKDWLRFAVAWWHTLGQASGDQ AcidFGGQTRNYAWDEPECPVERAKAKADAGFELMQKLGIEYFCFHDVDLIEEAATIEEYEERMGIITDYLLGKMKETGIKNLWGTANVFGHKRYMNGAATNPDFDVVARAAVQIKNAIDATIKLGGENYVFWGGREGYASLLNTQMQREKDHLGRMLAAARDYGRAHGFKGTFLIEPKPMEPTKHQYDQDTETVIAFLRRNGLDKDFKVNIEVNHATLAGHTFEHELAVAVDNGLLGSIDANRGDAQNGWDTDQFPIDNFELTQAMLQIIRNGGLGTGGSNFDAKLRRNSTDPEDIFIAHISAMDAMARALANAAAIIEESPIPAMLKERYASFDSGKGKEFEDGKLSLEELVAYAKANGEPKQISGKQELYETIVALYCK 5609MI1_005 Prevotella DNA 147ATGGCACAAGAATACTTCCCTACCATTGGGAAAATCCCCTTCGAGGGCACTGAGAGCAAGAATCCCCTTGCTTTCCATTACTATGAGCCGGAGCGCATTGTCTGCGGCAAACCCATGAAAGAATGGCTCAAGTTTGCCATGGCCTGGTGGCACACGCTGGGGCAGGCATCGGCCGATCCCTTCGGCGGCCAAACCCGCAGCTATGCCTGGGATAAGGGCGAATGCCCCTACTGCCGTGCCCGCGCCAAGGCGGACGCCGGCTTCGAGATTATGCAAAAGCTGGGCATCGAGTACTTCTGCTTCCACGATATCGACCTGGTAGAAGACTGTGACGATATTGCGGAATACGAAGCCCGCATGAAGGACATCACGGACTACCTCCTGGAGAAGATGAAGGAAACCGGTATCAAGAACCTCTGGGGCACCGCCAATGTGTTTGGTCACAAGCGCTACATGAACGGCGCCGCCACCAACCCGCAGTTTGACGTAGTGGCCCGTGCCGCTGTTCAGATTAAGAACGCCATTGACGCCACCATCAAGTTGGGCGGTGCCAATTACGTGTTCTGGGGCGGCCGCGAGGGCTATTACAGCCTCCTGAACACCCAGATGCAGCGGGAGAAGGACCACCTGGCCAAGCTGCTCACGGCAGCCCGCGACTATGCCCGCGCCAACGGCTTCAAGGGAACCTTCCTGATTGAGCCCAAGCCCATGGAGCCCACCAAGCACCAGTACGACGTGGATACGGAGACGGTCATTGGCTTCCTCCGCGCCAACGGCCTGGACAAGGACTTCAAGGTGAATATCGAGGTGAACCACGCCACGTTGGCCGGCCACACCTTTGAGCACGAGCTCACCGTGGCCCGCGAGAACGGCTTCCTGGGCAGCATCGACGCCAACCGCGGCGATGCCCAGAACGGCTGGGATACGGACCAGTTCCCGGTAGACGCTTATGAGCTCACCCAGGCCATGATGCAGGTGCTCCTGAACGGAGGCTTCGGCAACGGCGGCACCAACTTCGACGCCAAGCTGCGCCGCTCCTCCACGGACCCGGAGGACATCTTCATCGCCCATATCAGTGCGATGGATGCCATGGCCCACGCCCTGCTCAACGCCGCCGCCGTGCTGGAGGAAAGCCCCCTGTGCCAGATGGTGAAGGAGCGCTACGCCAGCTTTGACAGCGGTCCGGGCAAGCAGTTCGAGGAAGGAAAGGCCACCCTGGAGGACCTGTACAACTACGCCAAAGCCACCGGTGAACCCGTGGTTGCCTCCGGCAAGCAGGAACTTTACGAGACCCTCCTGAACCTCTATGCAAAGTAG 5609MI1_005Prevotella Amino 148MAQEYFPTIGKIPFEGTESKNPLAFHYYEPERIVCGKPMKEWLKFAMAWWHTLGQASADP AcidFGGQTRSYAWDKGECPYCRARAKADAGFEIMQKLGIEYFCFHDIDLVEDCDDIAEYEARMKDITDYLLEKMKETGIKNLWGTANVFGHKRYMNGAATNPQFDVVARAAVQIKNAIDATIKLGGANYVFWGGREGYYSLLNTQMQREKDHLAKLLTAARDYARANGFKGTFLIEPKPMEPTKHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANRGDAQNGWDTDQFPVDAYELTQAMMQVLLNGGFGNGGTNFDAKLRRSSTDPEDIFIAHISAMDAMAHALLNAAAVLEESPLCQMVKERYASFDSGPGKQFEEGKATLEDLYNYAKATGEPVVASGKQELYETLLNLYAK 5610MI1_003 Prevotella DNA 149ATGGCACAAGAATACTTCCCTACCATTGGGAAAATCCCCTTCGAGGGCACTGAGAGCAAGAATCCCCTTGCTTTCCATTACTATGAGCCGGAGCGCATTGTCTGCGGCAAACCCATGAAAGAATGGCTCAAGTTTGCCATGGCCTGGTGGCACACGCTGGGGCAGGCATCGGCCGATCCCTTCGGCGGCCAAACCCGCAGCTATGCCTGGGATAAGGGCGAATGCCCCTACTGCCGTGCCCGTGCCAAGGCGGACGCCGGTTTTGAGATTATGCAAAAGCTGGGCATCGAGTACTTCTGCTTCCACGATATCGACCTGGTAGAAGACTGTGACGATATTGCGGAATACGAAGCCCGCATGAAGGACATCACGGACTACCTCCTGGAGAAGATGAAGGAAACCGGCATCAAGAACCTCTGGGGCACCGCCAATGTGTTTGGTCACAAGCGCTACATGAACGGCGCCGGCACCAATCCGCAGTTTGACGTGGTGGCCCGTGCTGCCGTGCAAATCAAGAACGCCATTGACGCCACCATCAAGTTGGGCGGTGCCAATTACGTGTTCTGGGGCGGCCGCGAGGGCTATTACAGCCTCCTGAACACCCAGATGCAGCGGGAGAAGGACCACCTGGCCAAGCTGCTCACGGCAGCCCGCGACTATGCCCGCGCCAACGGCTTCAAGGGAACCTTCCTGATTGAGCCCAAGCCCATGGAGCCCACCAAGCACCAGTACGACGTGGATACGGAGACGGTCATTGGCTTCCTCCGCGCCAACGGCCTGGACAAGGACTTCAAGGTGAATATCGAGGTGAACCACGCCACGCTGGCCGGCCACACCTTTGAGCACGAACTCACCGTGGCCCGCGAGAACGGCTTCCTGGGCAGCATCGACGCCAACCGCGGCGATGCCCAGAACGGCTGGGATACGGACCAGTTCCCGGTAGACGCTTATGAGCTCACCCAGGCCATGATGCAGGTGCTCCTGAACGGAGGCTTCGGCAACGGCGGCACCAACTTCGACGCCAAGCTGCGCCGCTCCTCCACGGACCTGGAGGACATCTTCATCGCCCATATCAGTGCGATGGATGCCATGGCCCACGCCCTGCTCAACGCCGCCGCCGTGCTGGAGGAAAGCCCCCTGTGCCAGATGGTGAAGGAGCGCTACGCCAGCTTTGACAGCGGTCCGGGCAAGCAGTTCGAGGAAGGAAAGGCCACCCTGGAGGACCTGTACAACTACGCCAAAGCCAACGGTGAACCCGTGGTTGCCTCCGGCAAGCAGGAACTTTACGAGACCCTCCTGAACCTCTATGCAAAGTAG 5610MI1_003Prevotella Amino 150MAQEYFPTIGKIPFEGTESKNPLAFHYYEPERIVCGKPMKEWLKFAMAWWHTLGQASADP AcidFGGQTRSYAWDKGECPYCRARAKADAGFEIMQKLGIEYFCFHDIDLVEDCDDIAEYEARMKDITDYLLEKMKETGIKNLWGTANVFGHKRYMNGAGTNPQFDVVARAAVQIKNAIDATIKLGGANYVFWGGREGYYSLLNTQMQREKDHLAKLLTAARDYARANGFKGTFLIEPKPMEPTKHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANRGDAQNGWDTDQFPVDAYELTQAMMQVLLNGGFGNGGTNFDAKLRRSSTDLEDIFIAHISAMDAMAHALLNAAAVLEESPLCQMVKERYASFDSGPGKQFEEGKATLEDLYNYAKANGEPVVASGKQELYETLLNLYAK 5610MI2_004 Prevotella DNA 151ATGGCAAAAGAATATTTCCCTACCATCGGCAAGATTCCTTTTGAAGGAACCGACAGCAAGAGTCCCCTCGCCTTCCATTACTATGACGCCCAGCGCGTTGTGATGGGCAAACCCATGAAGGAATGGCTCAAGTTCGCCATGGCCTGGTGGCACACCCTGGGCCAGGCATCGGCCGACCCCTTCGGCGGTCAGACCCGCCACTATGCCTGGGATGAAGGCGAATGCCCCTACTGCCGCGCCAAAGCCAAGGCCGACGCCGGCTTCGAGATCATGCAGAAACTGGGCATCGAGTACTTCTGCTTCCACGATGTGGACCTGGTGGAAGACTGCGACGACATCGCCGAGTACGAAGCCCGCATGAAGGACATCACGGACTACCTGCTGGAGAAGATGAAGGAAACCGGCATCAAGAACCTCTGGGGCACGGCCAATGTGTTCGGCCACAAGCGTTACATGAACGGCGCCGGGACCAACCCGCAGTTTGACATTGTGGCCCGCGCTGCCGTCCAGATCAAAAACGCCCTGGACGCCACCATCAAGCTGGGCGGTTCCAACTACGTGTTCTGGGGCAGCCGCGAAGGCTACTACACCCTCCTGAACACCCAGATGCAGCGGGAGAAAGACCACCTGGCCAAGCTCCTGACCGCCGCCCGCGACTACGCCCGCGCCAAAGGCTTCAAGGGAACCTTCCTCATCGAGCCCAAACCCATGGAGCCCACCAAGCACCAGTACGACGTGGACACCGAGACCGTAATCGGCTTCCTGCGTGCCAACGGCCTGGACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCCACCCTGGCTGGCCACACCTTCGAGCACGAACTCACCGTCGCCCGTGAAAACGGCTTCCTCGGATCGATCGACGCCAACCGCGGCGACGCCCAGAACGGCTGGGACACCGACCAGTTCCCCGTAGACGCCTATGACCTCACCCAGGCCATGATGCAGGTGCTGCTGAACGGCGGTTTCGGCAATGGCGGTACCAACTTCGACGCCAAGCTCCGCCGCTCCTCCACGGATCCGGAAGACATCTTCATCGCCCACATCAGCGCCATGGACGCCATGGCCCACGCCCTGCTGAACGCCGCCGCCGTGCTGGAAGAAAGCCCGCTTCCCGCCATGGCGAAAGAGCGCTACGCCTCCTTTGACAGCGGACTTGGCAAGAAGTTCGAAGAGGGAAAGGCCACCCTCGAAGAGCTGTACGACTATGCCAAGGCTAACGACGCCCCTGTCGCCGCCTCCGGCAAGCAGGAACTTTACGAAACCTTCTTGAACCTCTATGCAAAATAG 5610MI2_004Prevotella Amino 152MAKEYFPTIGKIPFEGTDSKSPLAFHYYDAQRVVMGKPMKEWLKFAMAWWHTLGQASADP AcidFGGQTRHYAWDEGECPYCRAKAKADAGFEIMQKLGIEYFCFHDVDLVEDCDDIAEYEARMKDITDYLLEKMKETGIKNLWGTANVFGHKRYMNGAGTNPQFDIVARAAVQIKNALDATIKLGGSNYVFWGSREGYYTLLNTQMQREKDHLAKLLTAARDYARAKGFKGTFLIEPKPMEPTKHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANRGDAQNGWDTDQFPVDAYDLTQAMMQVLLNGGFGNGGTNFDAKLRRSSTDPEDIFIAHISAMDAMAHALLNAAAVLEESPLPAMAKERYASFDSGLGKKFEEGKATLEELYDYAKANDAPVAASGKQELYETFLNLYAK 5751MI1_003 Prevotella DNA 153ATGGCAAAACAGTATTTTCCGCAAATCGGAAAGATTAAATTCGAAGGAACAGAGAGCAAGAATCCGCTTGCGTTCCATTATTATGACGCAAACAGGGTAGTCCTCGGAAAGGCAATGGAGGAGTGGCTCAAGTTCGCAATGGCTTGGTGGCATACTCTCGGACAGGCTTCCGGAGACCAGTTCGGCGGCCAGACCCGCAGCTACGAGTGGGATCTTGCAGCCACCCCCGAGCAGCGCGCAAAGGACAAGCTCGACGCCGGCTTCGAAATAATGGAGAAACTTGGAATCAAGTATTTCTGTTTCCACGATGTTGACCTTATCGAAGACAGCGACGATATTGCGACATATGAGGCTCGTCTCAAGGACCTTACAGACTACGCTGCAGAGCAGATGAAGCTCCACGACATCAAGCTCCTCTGGGGTACAGCGAATGTATTCGGCAACAAGCGCTACATGAACGGTGCGGCTACAAACCCTGATTTCGATGTAGTTGCCCGCGCAGCCGTTCAGATTAAGAACGCTATCGACGCGACCATCAAGCTCGGTGGTACCAGCTATGTATTCTGGGGCGGTCGTGAGGGATATCAGAGCCTGCTCAACACTCAGATGCAGCGTGAGAAGGACCACCTCGCAACCATGCTTACAATCGCTCGCGACTATGCTCGCAGCAAGGGCTTTACCGGAACCTTCCTTATCGAGCCTAAGCCGATGGAGCCTACAAAACACCAGTACGACGTAGATACAGAGACTGTTGTCGGCTTCCTCAAGGCACACGGCCTGGACAAGGACTTCAAGGTAAATATCGAGGTTAACCACGCAACTCTCGCAGGCCACACCTTCGAGCACGAACTCACCGTTGCTGTGGATAACGGAATGCTCGGTTCTATCGACGCTAACCGCGGTGATGCACAGAACGGCTGGGATACAGACCAGTTCCCTGTAAGCGCTGAGGAGCTTACCCTCGCTATGATGCAGATTATCCGTAATGGTGGCCTTGGCAACGGAGGATCCAACTTCGACGCAAAGCTTCGCCGCAACTCTACCGATCCTGAAGACATCTTCATCGCACACATCTGCGGTATGGATGCAATGGCACACGCTCTCCTCAATGCAGCTGCAATTATCGAGGAGTCTCCTATCCCTACAATGGTTAAGGAGCGTTACGCTTCCTTCGACAGCGGTATGGGTAAGGACTTCGAGGATGGAAAGCTTACCCTCGAGGATCTCTACAGCTACGGCGTGAAGAACGGAGAGCCAAAGCAGACCAGCGCAAAGCAGGAGCTCTATGAGACTCTCATGAATATCTATTGCAAGTAA 5751MI1_003Prevotella Amino 154MAKQYFPQIGKIKFEGTESKNPLAFHYYDANRVVLGKAMEEWLKFAMAWWHTLGQASGDQ AcidFGGQTRSYEWDLAATPEQRAKDKLDAGFEIMEKLGIKYFCFHDVDLIEDSDDIATYEARLKDLTDYAAEQMKLHDIKLLWGTANVFGNKRYMNGAATNPDFDVVARAAVQIKNAIDATIKLGGTSYVFWGGREGYQSLLNTQMQREKDHLATMLTIARDYARSKGFTGTFLIEPKPMEPTKHQYDVDTETVVGFLKAHGLDKDFKVNIEVNHATLAGHTFEHELTVAVDNGMLGSIDANRGDAQNGWDTDQFPVSAEELTLAMMQIIRNGGLGNGGSNFDAKLRRNSTDPEDIFIAHICGMDAMAHALLNAAAIIEESPIPTMVKERYASFDSGMGKDFEDGKLTLEDLYSYGVKNGEPKQTSAKQELYETLMNIYCK 5751MI2_003 Prevotella DNA 155ATGGCAAAAGAATTTTTTCCACAAGTAGGCAAGATTCCATTTGAGGGTCCTGAAAGTACTAACGTACTCGCATTCCACTACTATGATCCAGAACGCGAAGTTCTTGGTAAGAAAATGAAAGATTGGCTGAAGTATGCTATGGCTTGGTGGCACACACTCGGTCAGGCAAGTGGCGACCAATTCGGTGGTCAAACTCGTTCGTATGAATGGGATGAAGCCGACGATGTTCTTCAACGCGCAAAGGATAAAATGGATGCTGGTTTTGAATTGATGACCAAACTTGGCATTGAATACTACTGCTTCCATGATGTCGACCTTATTGAAGAAGGTGCAACAATTGAAGAATATGAAGCTCGTATGCAAGCTATCACCGACTACGCATTAGAAAAACAAAAAGAAACCGGCATTAAGCTCCTTTGGGGTACTGCTAATGTGTTTGGTCATAAGCGTTATATGAATGGTGCGGCAACAAACCCTGACTTTGATGTAGTGGCTCGCGCTGCTGTACAAATCAAGAACGCTATCGATGCAACTATCAAGCTTGGTGGTCAAAACTATGTATTCTGGGGTGGCCGCGAAGGTTATATGAGTTTGCTCAACACTCAAATGCAACGCGAAAAAGACCACTTGGCAAAGATGCTTACCGCAGCTCGCGACTATGCTCGTGCTAAGGGCTTCAAGGGTACATTCCTCGTTGAACCTAAGCCTATGGAACCAACTAAGCATCAATATGATACCGATACAGAAACTGTGATTGGTTTCCTCCGTGCAAATGGTCTTGAAAAAGACTTCAAGGTGAACATTGAAGTGAACCATGCTACTCTCGCTCAGCACACTTTCGAACACGAACTCGCTGTGGCTGTCGACAATGGCATGCTCGGTTCTATCGACGCTAACCGTGGCGATGCTCAAAATGGCTGGGATACCGACCAATTCCCAATCGACAACTACGAACTCACCCTCGCTATGCTCCAAATCATTCGCAATGGTGGTCTTGGCAATGGCGGTAGCAACCTCGACGCTAAGATTCGTCGTAATAGCACCGACCTTGAAGACCTCTTTATCGCTCACATCAGTGGTATGGATGCTATGGCTCGTGCACTTCTCAATGCTGCTGCAATCGTTGAAAAGAGCGAAATTCCTGCTATGTTGAAGCAGCGTTATGCAAGCTCTGATGCAGGTATGGGTAAGGACTTCGAAGAAGGAAAACTCACTCTCGAACAACTCGTAGACTATGCTAAGGCTAACGGCGAACCTGCTACAGTAAGCGGCAAGCAAGAAAAGTATGAAACTCTCGTTGCTCTCTACGCTAAGTAA 5751MI2_003Prevotella Amino 156MAKEFFPQVGKIPFEGPESTNVLAFHYYDPEREVLGKKMKDWLKYAMAWWHTLGQASGDQ AcidFGGQTRSYEWDEADDVLQRAKDKMDAGFELMTKLGIEYYCFHDVDLIEEGATIEEYEARMQAITDYALEKQKETGIKLLWGTANVFGHKRYMNGAATNPDFDVVARAAVQIKNAIDATIKLGGQNYVFWGGREGYMSLLNTQMQREKDHLAKMLTAARDYARAKGFKGTFLVEPKPMEPTKHQYDTDTETVIGFLRANGLEKDFKVNIEVNHATLAQHTFEHELAVAVDNGMLGSIDANRGDAQNGWDTDQFPIDNYELTLAMLQIIRNGGLGNGGSNLDAKIRRNSTDLEDLFIAHISGMDAMARALLNAAAIVEKSEIPAMLKQRYASSDAGMGKDFEEGKLTLEQLVDYAKANGEPATVSGKQEKYETLVALYAK 5752MI1_003 Prevotella DNA 157ATGACTAAAGAGTATTTCCCGGGAATCGGAAAGATTCCGTTTGAAGGAACCAAGAGCAAGAACCCCCTGGCCTTCCATTATTATAACGCCTCCCAGGTAGCGATGGGCAAGCCCATGAAGGACTGGCTCAAGTATGCCATGGCCTGGTGGCACACCCTGGGCCAGGCCTCTGCAGACCCCTTTGGCGGCCAGACCCGCTCCTACGAATGGGACAAGGGCGAGTGCCCTTATTGCCGCGCCAAGCAGAAGGCCGATGCCGGCTTTGAGCTCATGCAGAAGCTGGGCATCGAGTACTACTGCTTCCACGACGTGGACATCATCGAGGACTGCGAGGACATTGCCGAGTACGAGGCCCGCATGAAGGACATCACGGACTACCTGCTGGAGAAGCAGAAAGAGACCGGCATCAAGAACCTCTGGGGCACCGCCAACGTGTTTGGCCACAAGCGCTACATGAACGGCGCCGCCACCAACCCTCAGTTTGACATTGTGGCCCGTGCCGCCGTCCAGATCAAGAACGCCCTGGATGCCACCATCAAGCTGGGTGGTACCAACTACGTGTTCTGGGGTGGCCGCGAAGGCTACTACACGCTGCTCAACACCCAGATGCAGCGGGAGAAGAACCACCTGGCCAAGATGCTCACCGCCGCCCGCGACTACGCCCGCGCCAAGGGCTTCAAGGGCACCTTCCTCATTGAGCCCAAACCCATGGAGCCCACCAAGCACCAGTACGACGTGGACACCGAGACCGTGATTGGTTTCATCCGCGCCAACGGCCTGGACAAGGACTTCAAGGTAAACATTGAGGTAAACCACGCCACCCTGGCCGGCCACACCTTTGAGCACGAGCTCACCGTGGCCCGCGAGAACGGCTTCCTGGGCTCCATCGACGCCAACCGCGGAGATGCCCAGAACGGCTGGGATACGGACCAGTTCCCCATCGACGCCCTGGATCTCACCCAGGCTATGATGCAGGTCATCCTCAACGGTGGCTTCGGCAATGGCGGCACCAACTTTGACGCCAAGCTCCGCCGCTCCTCCACCGATCCCGAGGACATCTTCATCGCCCACATCAGCGCCATGGATGCCATGGCACACGCCCTCCTGAACGCAGCCGCCATCCTGGAAGAGAGCCCCCTGCCCGCCATGGTCAAGGAGCGTTACGCTTCCTTCGACAGCGGTCTGGGCAAGAAGTTCGAAGAAGGCAAGGCCTCCCTGGAAGAACTTTACGAATATGCCAAGAAGAATGGAGAGCCCGTGGCCGCTTCCGGCAAACAGGAGCTCTGCGAAACTTACTTGAACCTCTATGCAAAGTAG 5752MI1_003Prevotella Amino 158MTKEYFPGIGKIPFEGTKSKNPLAFHYYNASQVAMGKPMKDWLKYAMAWWHTLGQASADP AcidFGGQTRSYEWDKGECPYCRAKQKADAGFELMQKLGIEYYCFHDVDIIEDCEDIAEYEARMKDITDYLLEKQKETGIKNLWGTANVFGHKRYMNGAATNPQFDIVARAAVQIKNALDATIKLGGTNYVFWGGREGYYTLLNTQMQREKNHLAKMLTAARDYARAKGFKGTFLIEPKPMEPTKHQYDVDTETVIGFIRANGLDKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANRGDAQNGWDTDQFPIDALDLTQAMMQVILNGGFGNGGTNFDAKLRRSSTDPEDIFIAHISAMDAMAHALLNAAAILEESPLPAMVKERYASFDSGLGKKFEEGKASLEELYEYAKKNGEPVAASGKQELCETYLNLYAK 5752MI2_003 Prevotella DNA 159ATGACTAAAGAGTATTTCCCGGGAATCGGAAAGATTCCGTTTGAAGGAACCAAGAGCAAGAACCCCCTGGCCTTCCATTATTATAACGCCTCCCAGGTAGTGATGGGCAAGCCCATGAAGGACTGGCTCAAGTATGCCATGGCCTGGTGGCACACCCTGGGCCAGGCCTCTGCAGACCCCTTTGGCGGCCAGACCCGCTCCTACGAATGGGACAAGGGCGAGTGCCCGTACTGCCGCGCCAAGCAGAAGGCCGATGCCGGCTTTGAGCTCATGCAGAAGCTGGGCATCGAGTACTACTGCTTCCACGACGTGGACATCATCGAGGACTGCGAGGACATTGCCGAGTACGAGGCCCGCATGAAGGACATCACGGACTACCTGCTGGAGAAGCAGAAAGAGACCGGCATCAAGAACCTCTGGGGCACCGCCAACGTGTTTGGCCACAAGCGCTACATGAACGGCGCCGCCACCAACCCTCAGTTTGACATTGTGGCCCGTGCCGCCGTCCAGATCAAGAACGCCCTGGATGCCACCATCAAACTGGGTGGTACCAACTACGTGTTCTGGGGTGGCCGCGAAGGCTACTACACGCTGCTCAACACCCAGATGCAGCGGGAGAAGAACCACCTGGCCAAGATGCTCACCGCCGCCCGCGACTACGCCCGCGCCAAGGGCTTCAAGGGCACCTTCCTCATTGAGCCCAAACCCATGGAGCCCACCAAGCACCAGTACGACGTGGACACCGAGACCGTGATTGGTTTCATCCGCGCCAACGGCCTGGACAAGGACTTCAAGGTAAACATTGAGGTAAACCACGCCACCCTGGCCGGCCACACCTTTGAGCACGAGCTCACCGTGGCCCGCGAGAACGGCTTCCTGGGCTCCATCGACGCCAACCGCGGAGATGCCCAGAACGGCTGGGATACGGACCAGTTCCCCATCGACGCCCTGGATCTCACCCAGGCTATGATGCAGGTCATCCTCAACGGTGGCTTCGGCAATGGCGGCACCAACTTTGACGCCAAGCTCCGCCGCTCCTCCACCGATCCCGAGGACATCTTCATCGCCCACATCAGCGCCATGGATGCCATGGCACACGCCCTCCTGAACGCAGCCGCCATCCTGGAAGAGAGCCCCCTGCCCGCCATGGTCAAGGAGCGTTACGCTTCCTTCGACAGCGGTCTGGGCAAGAAGTTCGAAGAAGGCAAGGCCTCCCTGGAAGAACTTTACGAATATGCCAAGAAGAATGGAGAGCCCGTGGCCGCTTCCGGCAAACAGGAGCTCTGCGAAACTTACTTGAACCTCTATGCAAAGTAG 5752MI2_003Prevotella Amino 160MTKEYFPGIGKIPFEGTKSKNPLAFHYYNASQVVMGKPMKDWLKYAMAWWHTLGQASADP AcidFGGQTRSYEWDKGECPYCRAKQKADAGFELMQKLGIEYYCFHDVDIIEDCEDIAEYEARMKDITDYLLEKQKETGIKNLWGTANVFGHKRYMNGAATNPQFDIVARAAVQIKNALDATIKLGGTNYVFWGGREGYYTLLNTQMQREKNHLAKMLTAARDYARAKGFKGTFLIEPKPMEPTKHQYDVDTETVIGFIRANGLDKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANRGDAQNGWDTDQFPIDALDLTQAMMQVILNGGFGNGGTNFDAKLRRSSTDPEDIFIAHISAMDAMAHALLNAAAILEESPLPAMVKERYASFDSGLGKKFEEGKASLEELYEYAKKNGEPVAASGKQELCETYLNLYAK 5752MI3_002 Prevotella DNA 161ATGGCAAAAGAGTATTTCCCGACTATCGGCAAGATTCCCTTCGAGGGCGTCGAATCCAAGAACCCGATGGCATTCCACTACTATGACGCGAACCGCGTCGTGATGGGCAAGCCCATGAAGGACTGGCTCAAGTTCGCGATGGCCTGGTGGCACACCCTGGGACAGGCTTCCGGCGACCCGTTCGGCGGCCAGACCCGTTCCTACGAGTGGGACAAGGGCGAGTGCCCCTACTGCCGCGCCAAGGCCAAGGCCGACGCCGGCTTCGAGATCATGCAGAAGCTCGGTATCGAGTACTACTGCTTCCATGACATCGACCTCGTGGAGGACACCGAGGACATCGCCGAGTACGAGGCCCGCATGAAGGACATCACCGACTACCTCGTCGAGAAGCAGAAGGAAACCGGCATCAAGAACCTCTGGGGCACGGCCAACGTGTTCGGCAACAAGCGCTACATGAACGGCGCCGCCACGAACCCGCAGTTCGACGTCGTCGCCCGCGCCGCCGTCCAGATCAAGAACGCCATCGACGCCACCATCAAGCTCGGCGGTACCGGTTACGTGTTCTGGGGCGGCCGTGAAGGCTACTACACCCTCCTGAACACCCAGATGCAGCGCGAGAAGGACCACCTCGCCAAGATGCTCACCGCCGCCCGCGACTACGCCCGCGCCCACGGCTTCCAGGGCACCTTCCTCATCGAGCCCAAGCCCATGGAGCCCACCAAGCACCAGTACGACGTGGACACGGAGACCGTGATCGGCTTCCTGCGCGCCAACGGTCTGGACAAGGACTTCAAGGTCAATATCGAGGTGAACCACGCCACCCTCGCCGGCCACACCTTCGAGCACGAGCTCACCGTGGCTGTCGATAACGGCTTCCTCGGCTCCATCGACGCCAACCGCGGCGACGCCCAGAACGGCTGGGACACCGACCAGTTCCCCGTGGACCCGTACGACCTCACCCAGGCCATGATGCAGATCATCCGCAACGGCGGTTTCAAGGACGGCGGCACCAACTTCGACGCCAAGCTCCGCCGCTCTTCCACCGACCCGGAGGACATCTTCATCGCCCACATCAGCGCGATGGACGCCATGGCCCACGCCCTGCTGAACGCCGCCGCCGTCATCGAGGAGAGCCCGCTCTGCAAGATGGTCGAGGAGCGCTACGCTTCCTTCGACAGCGGCCTCGGCAAGCAGTTCGAGGAAGGCAAGGCCACCCTCGAGGACCTCTACGAGTATGCCAAGAAGAATGGCGAGCCCGTCGTCGCCTCCGGCAAGCAGGAGCTCTACGAGACGCTGCTGAACCTTTACGCGAAGTAG 5752MI3_002Prevotella Amino 162MAKEYFPTIGKIPFEGVESKNPMAFHYYDANRVVMGKPMKDWLKFAMAWWHTLGQASGDP AcidFGGQTRSYEWDKGECPYCRAKAKADAGFEIMQKLGIEYYCFHDIDLVEDTEDIAEYEARMKDITDYLVEKQKETGIKNLWGTANVFGNKRYMNGAATNPQEDVVARAAVQIKNAIDATIKLGGTGYVFWGGREGYYTLLNTQMQREKDHLAKMLTAARDYARAHGFQGTFLIEPKPMEPTKHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVAVDNGFLGSIDANRGDAQNGWDTDQFPVDPYDLTQAMMQIIRNGGFKDGGTNFDAKLRRSSTDPEDIFIAHISAMDAMAHALLNAAAVIEESPLCKMVEERYASFDSGLGKQFEEGKATLEDLYEYAKKNGEPVVASGKQELYETLLNLYAK 5752MI5_003 Prevotella DNA 163ATGGCAAAAGAGTATTTCCCGACAATCGGTAAGATCCCCTTCGAGGGACCCGAGTCCAAGAACCCGATGGCATTCCACTACTATGACGCGGAGCGCGTGGTGATGGGCAAGAAGATGAAGGACTGGTTCAAGTTCGCGATGGCCTGGTGGCACACCCTGGGCCAGGCTTCCGCCGACCCGTTCGGCGGCCAGACCCGCTCCTACGAGTGGGACAAGGGCGAAGGCCCCTGCTCCCGCGCCCGCGCCAAGGCTGACGCCGGTTTCGAGATCATGCAGAAACTGGGCATCGGCTACTACTGCTTCCACGACATCGACCTGGTGGAGGACACCGAGGACATCGCCGAGTATGAAGCCCGCATGAAGGACATCACCGACTACCTCGTGGAGAAGCAGAAGGAGACCGGCATCAAGAACCTCTGGGGCACGGCCAACGTATTCGGCAACAAGCCCTACATGAACGGCGCCGCCACGAACCCGCAGTTCGACATCGCCGCCCGCGCGGCCCTGCAGACCAAGAACGCCATCGATGCCACCATCAAGCTGGGCGGCACCGGTTACGTGTTCTGGGGCGGCCGTGAAGGCTACTACACCCTCCTGAACACCCAGATGCAGCGCGAGAAGGACCACCTTGCCAAGATGCTCACCGCGGCTCGCGACTATGCCCGCGCCCACGGCTTCAAGGGCACCTTCTTCATCGAGCCGAAACCGATGGAGCCCACCAAGCACCAGTACGACGTGGACACGGAGACCGTGATCGGCTTCCTCCGCGCCAACGGCCTGGACAAGGACTTCAAGGTGAACATCGAAGTGAACCACGCCACCCTCGCCGGCCACACCTTCGAGCACGGGCTCACCGTGGCCGTTGACAACGGCTTCCTCGGCAGCATCGACGCCAACCGCGGAGACGCCCAGAACGGCTGGGATACCGACCAGTTCCCGGTGGATCCGTACGACCTCACCCAGGCGATGATCCAGATCATCCGCAATGGCGGCTTCAAGGACGGCGGTACCAACTTCGACGCCAAGCTCCGCCGCTCTTCCACCGACCCGGAGGACATCTTCATCGCCCACATCAGCGCGATGGACGCCATGGCCCACGCCCTGCTGAACGCCGCCGCCGTGCTCGAGGAGAGCCCGCTCTGCGAGATGGTTGCAAAGCGTTACGCTTCCTTCGACAGCGGTCTCGGCAAGAAGTTCGAGGAAGGCAACGCCACCCTCGAGGAACTCTACGAGTACGCCAAGGCGAAGGGCGAGGTCGTTGCCGAATCCGGCAAGCAGGAACTCTACGAGACCCTGCTGAACCTCTACGCGAAGTAG 5752MI5_003Prevotella Amino 164MAKEYFPTIGKIPFEGPESKNPMAFHYYDAERVVMGKKMKDWFKFAMAWWHTLGQASADP AcidFGGQTRSYEWDKGEGPCSRARAKADAGFEIMQKLGIGYYCFHDIDLVEDTEDIAEYEARMKDITDYLVEKQKETGIKNLWGTANVFGNKPYMNGAATNPQFDIAARAALQTKNAIDATIKLGGTGYVFWGGREGYYTLLNTQMQREKDHLAKMLTAARDYARAHGFKGTFFIEPKPMEPTKHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHGLTVAVDNGFLGSIDANRGDAQNGWDTDQFPVDPYDLTQAMIQIIRNGGFKDGGTNFDAKLRRSSTDPEDIFIAHISAMDAMAHALLNAAAVLEESPLCEMVAKRYASFDSGLGKKFEEGNATLEELYEYAKAKGEVVAESGKQELYETLLNLYAK 5752MI_6004 Prevotella DNA 165ATGGCAAAAGAGTATTTCCCGACAATCGGAAAGATCCCCTTCGAGGGCGCTGAGAGCAAGAATCCCCTTGCTTTCCACTATTATGACGCCGAGCGTGTGGTCATGGGCAAGCCCATGAAGGACTGGTTCAAGTTCGCGATGGCCTGGTGGCACACCCTGGGCCAGGCTTCCGCCGACCCGTTCGGCGGCCAGACCCGCTCCTACGAGTGGGACAAGGGCGAGTGCCCCTACTGCCGCGCCCGCCAGAAGGCTGACGCCGGTTTCGAGATCATGCAGAAGCTCGGCATCGGCTACTACTGCTTCCACGACATCGACCTGGTCGAGGACACCGAGGACATCGCCGAGTACGAGGCCCGCATGAAGGACATCACCGACTACCTCGTCGAGAAGCAGAAGGAGACCGGCATCAAGAACCTCTGGGGCACGGCCAACGTGTTCGGCAACAAGCGCTACATGAACGGCGCCGCCACGAACCCGCAGTTCGACATCGTCGCCCACGCGGCCCTGCAGATCAAGAACGCGATCGGCGCCACCATCAAGCTCGGCGGCACCGGTTACGTGTTCTGGGGCGGCCGTGAAGGTTACTACACCCTCCTGAACACCCAGATGCAGCGCGAGAAGGACCACCTCGCCAAGATGCTCACCGCCGCCCGCGACTACGCCCGCGCCAACGGCTTCAAGGGCACCTTCCTCATCGAGCCGAAGCCGATGGAGCCCACCAAGCACCAGTATGACGTGGACACGGAGACCGTGATCGGCTTCCTCCGCGCCAACGGCCTGGACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCCACCCTCGCCGGCCACACCTTCGAGCACGAGCTCACCGTGGCGGTCGACAACGGCTTCCTCGGCAGCATCGACGCCAACCGCGGTGACGCCCAGAACGGCTGGGATACCGACCAGTTCCCGGTGGATCCGTACGATCTCACCCAGGCGATGATCCAGATCATCCGCAACGGCGGCTTCAAGGATGGCGGCACCAACTTCGACGCCAAGCTCCGCCGCTCTTCCACCGACCCGGAGGACATCTTCATCGCCCACATCAGCGCGATGGACGCCATGGCCCACGCCCTGCTGAACGCCGCCGCCGTCATCGAGGAGAGCCCGCTCTGCGAGATGGTCGCCAAGCGCTACGCTTCCTTCGACAGCGGTCTCGGCAAGAAGTTCGAGGAAGGCAACGCCACCCTCGAGGAACTCTACGAGTACGCCAAGGCGAACGGTGAGGTCAAGGCCGAATCCGGCAAGCAGGAGCTCTACGAGACCCTTCTGAACCTCTACGCGAAATAG 5752MI6_004Prevotella Amino 166MAKEYFPTIGKIPFEGAESKNPLAFHYYDAERVVMGKPMKDWFKFAMAWWHTLGQASADP AcidFGGQTRSYEWDKGECPYCRARQKADAGFEIMQKLGIGYYCFHDIDLVEDTEDIAEYEARMKDITDYLVEKQKETGIKNLWGTANVEGNKRYMNGAATNPQFDIVAHAALQIKNAIGATIKLGGTGYVFWGGREGYYTLLNTQMQREKDHLAKMLTAARDYARANGFKGTFLIEPKPMEPTKHQYDVDTETVIGFLRANGLDKDFKVNIEVNHATLAGHTFEHELTVAVDNGFLGSIDANRGDAQNGWDTDQFPVDPYDLTQAMIQIIRNGGFKDGGTNFDAKLRRSSTDPEDIFIAHISAMDAMAHALLNAAAVIEESPLCEMVAKRYASFDSGLGKKFEEGNATLEELYEYAKANGEVKAESGKQELYETLLNLYAK 5753MI1_002 Prevotella DNA 167ATGGCAAAAGAGTATTTCCCCACTATCGGGAAGATTCCTTTCGAAGGAGTCGAGAGCAAGAACCCCCTTGCATTCCATTATTATGACGCAAACCGCATGGTCATGGGCAAGCCCATGAAGGACTGGTTCAAGTTCGCCATGGCATGGTGGCACACCCTGGGACAGGCCTCCGCAGACCCGTTCGGCGGCCAGACCCGCTCCTACGAATGGGACAAGGGCGAATGCCCCTACTGCCGCGCCAGGGCAAAGGCCGATGCCGGCTTCGAGATCATGCAGAAACTGGGTATCGAGTATTTCTGCTTCCATGACATCGACCTGGTAGAGGACTGCGACGACATCGCCGAGTACGAGGCCCGCATGAAGGACATCACGGACTATCTCCTGGAGAAGATGAAGGAAACCGGCATCAAGAACCTCTGGGGCACCGCCAACGTGTTCGGCAACAAGCGTTACATGAACGGCGCCGGCACCAATCCGCAGTTCGACGTAGTGGCCCGCGCTGCCGTCCAGATCAAGAACGCCATCGACGCCACCATCAAGCTCGGCGGTTCCAACTATGTGTTCTGGGGCGGCCGTGAAGGATACTACACCCTGCTGAACACCCAGATGCAGCGCGAGAAGGACCACCTCGGCAAACTGCTCACCGCCGCCCGCGACTATGCCCGCAAGAACGGCTTCAAGGGCACCTTCCTCATCGAGCCCAAGCCGATGGAGCCCACCAAGCACCAGTACGACGTAGACACGGAGACCGTGATCGGCTTCCTCCGCGCCAACGGCCTGGAGAAAGACTTCAAGGTGAACATCGAGGTGAACCACGCCACCCTGGCCGGCCATACCTTCGAGCATGAACTCACCGTGGCCGTGGACAACGGCTTCCTGGGATCCATCGACGCCAACCGCGGCGACGCCCAGAACGGCTGGGATACGGACCAGTTCCCGGTAGACCCGTACGACCTCACCCAGGCCATGATGCAGATCATCCGCAACGGCGGCCTCGGCAACGGCGGTACCAACTTCGACGCCAAACTGCGCCGTTCCTCCACCGATCCTGAGGACATCTTCATCGCCCACATCAGCGCCATGGACGCCATGGCCCACGCCCTGCTCAACGCAGCCGCCGTGCTGGAAGAAAGTCCGCTCTGTGAGATGGTCAAGGAGCGCTACGCTTCCTTCGACAGCGGTCTCGGCAAGAAGTTCGAAGAGGGCAAGGCTACCCTGGAAGAAATCTACGAGTATGCCAAGAAGAGCGGCGAACCCGTGGTCGCTTCCGGCAAGCAGGAGCTCTACGAAACCCTGCTGAACCTCTACGCCAAGTAG 5753MI1_002Prevotella Amino 168MAKEYFPTIGKIPFEGVESKNPLAFHYYDANRMVMGKPMKDWFKFAMAWWHTLGQASADP AcidFGGQTRSYEWDKGECPYCRARAKADAGFEIMQKLGIEYFCFHDIDLVEDCDDIAEYEARMKDITDYLLEKMKETGIKNLWGTANVFGNKRYMNGAGTNPQEDVVARAAVQIKNAIDATIKLGGSNYVFWGGREGYYTLLNTQMQREKDHLGKLLTAARDYARKNGFKGTFLIEPKPMEPTKHQYDVDTETVIGFLRANGLEKDFKVNIEVNHATLAGHTFEHELTVAVDNGFLGSIDANRGDAQNGWDTDQFPVDPYDLTQAMMQIIRNGGLGNGGTNFDAKLRRSSTDPEDIFIAHISAMDAMAHALLNAAAVLEESPLCEMVKERYASFDSGLGKKFEEGKATLEEIYEYAKKSGEPVVASGKQELYETLLNLYAK 5753MI2_002 Prevotella DNA 169ATGGCTAAAGAATACTTCCCCTCCATCGGCAAAATCCCTTTTGAAGGAGGCGACAGCAAAAATCCCCTCGCTTTCCATTATTATGACGCCGGACGCGTGGTTATGGGCAAGCCCATGAAGGAATGGCTTAAATTCGCCATGGCCTGGTGGCACACGCTGGGCCAGGCCTCCGGAGACCCCTTCGGCGGCCAGACCCGCAGCTACGAATGGGACAAGGGCGAATGCCCCTACTGCCGCGCCAAAGCCAAGGCCGACGCCGGTTTTGAAATCATGCAAAAGCTGGGTATCGAATACTTCTGCTTCCACGATGTGGACCTTATCGAGGATTGCGATGACATTGCCGAATACGAAGCCCGCATGAAGGACATCACGGACTACCTGCTGGAAAAGATGAAGGAGACCGGCATCAAGAACCTCTGGGGCACCGCCAATGTCTTCGGCCACAAGCGCTACATGAACGGCGCCGCCACGAACCCGCAGTTCGACGTGGTCGCCCGCGCCGCCGTCCAGATCAAGAACGCGATTGACGCCACCATCAAGCTCGGCGGTACCAGTTATGTATTCTGGGGCGGCCGCGAGGGCTACTACACCCTCCTGAACACCCAGATGCAGCGTGAGAAAGACCACCTGGCCAAGATGCTCACCGCAGCCCGCGACTACGCCCGCGCCAAGGGCTTCAAGGGCACCTTCCTCATCGAGCCCAAGCCGATGGAGCCCACCAAGCACCAGTACGACGTTGACACGGAGACCGTGATCGGCTCCCTGCGCGCCAACGGCCTGGACAAGGACTTCAAGGTGAACATCGAGGTGAACCACGCCACCCTGGCCGGCCACACCTTCGAGCACGAACTCACCGTGGCTGTTGACAACGGCTTCCTGGGCTCCATCGACGCCAACCGCGGCGACGCCCAGAACGGCTGGGATACGGACCAGTTCCCGGTAGACCCGTACGACCTCACCCAGGCCATGATGCAGATTATCCGCAACGGCGGCTTCAAGGACGGCGGCACCAACTTCGATGCCAAACTGCGCCGCTCTTCCACCGATCCGGAAGACATCTTCATCGCCCACATCAGCGCTATGGATGCCATGGCACACGCCCTGCTCAACGCCGCCGCCGTGCTGGAAGAGAGCCCGCTGTGCAACATGGTCAAGGAGCGTTACGCCGGCTTCGACAGCGGCCTTGGCAAGAAGTTCGAGGAAGGGAAGGCAACGCTGGAGGAAATCTATGACTATGCCAAGAAGAGCGGCGAACCCGTCGTGGCTTCCGGCAAGCAGGAACTCTACGAAACCATCCTGAACCTCTATGCCAAGTAG 5753MI2_002Prevotella Amino 170MAKEYFPSIGKIPFEGGDSKNPLAFHYYDAGRVVMGKPMKEWLKFAMAWWHTLGQASGDP AcidFGGQTRSYEWDKGECPYCRAKAKADAGFEIMQKLGIEYFCFHDVDLIEDCDDIAEYEARMKDITDYLLEKMKETGIKNLWGTANVFGHKRYMNGAATNPQFDVVARAAVQIKNAIDATIKLGGTSYVFWGGREGYYTLLNTQMQREKDHLAKMLTAARDYARAKGFKGTFLIEPKPMEPTKHQYDVDTETVIGSLRANGLDKDFKVNIEVNHATLAGHTFEHELTVAVDNGFLGSIDANRGDAQNGWDTDQFPVDPYDLTQAMMQIIRNGGFKDGGTNFDAKLRRSSTDPEDIFIAHISAMDAMAHALLNAAAVLEESPLCNMVKERYAGFDSGLGKKFEEGKATLEEIYDYAKKSGEPVVASGKQELYETILNLYAK 5753MI4_002 Prevotella DNA 171ATGTCAAAAGAGTATTTCCCTACAATCGGCAGGGTCCCCTTCGAGGGACCTGAGAGCAAGAATCCGCTGGCGTTCCACTATTACGAGCCGGACCGGCTCGTCCTGGGCAGGAAAATGAAGGACTGGCTGCGCTTCGCAATGGCCTGGTGGCATACGCTCGGGCAGGCTTCCGGCGACCAGTTCGGCGGACAGACCTGCACATACGCCTGGGATGAAGGCGAGTGTCCCGTCTGCCGGGCAAAGGCCAAGGCTGACGCCGGCTTTGAACTGATGCAGAAACTGGGCATCGGGTATTTCTGCTTCCACGACGTGGACCTGGTCGAGGAGGCCGACACCATTGAAGAATACGAGGAGCGGATGCGGATCATCACCGACTACCTGCTCGAGAAGATGGAAGAGACCGGCATCCGCAATCTCTGGGGAACCGCCAATGTCTTCGGACACAAGCGCTATATGAACGGCGCCGCCACCAATCCCGACTTCGACGTCGTGGCCCGTGCCGCGGTCCAGATCAAGAATGCCATCGATGCCACCATCAAACTGGGTGGTGAGAACTATGTGTTCTGGGGTGGCCGCGAGGGCTATACGAGCCTGCTCAACACGCAGATGCACCGGGAAAAACACCACCTCGGAAATATGCTCAGGGCAGCCCGCGACTATGGCCGTGCCCACGGTTTCAAGGGAACGTTCCTGATCGAGCCCAAGCCGATGGAGCCGACCAAGCATCAGTACGACCAGGATACGGAGACGGTCATCGGTTTCCTGCGCTGTCACGGCCTGGACAAGGATTTCAAGGTGAACATCGAGGTGAACCACGCCACGCTCGCCGGACACACCTTCGAGCACGAACTGGCCACTGCGGTCGATGCCGGCCTGCTGGGCAGCATCGATGCCAACCGCGGCGACGCCCAGAACGGCTGGGATACCGACCAGTTCCCGATCGACAACTACGAACTCACGCTGGCGATGCTGCAGATCATCCGCAATGGCGGACTCGCACCCGGCGGATCGAACTTCGATGCCAAGTTGCGCCGCAATTCCACCGATCCGGAAGACATCTTCATCGCCCACATCAGCGCGATGGACGCGATGGCCCGTGCCCTGCTCAATGCGGCGGCCATCTGGACCGAATCGCCGATTCAGGATATGGTCAGGGACCGCTATGCTTCCTTCGACAGCGGAAAGGGCAGGGAGTTCGAGGAAGGCAGACTCAGTCTGGAAGACCTCGTGGCCTATGCGAAGGAGCACGGTGAGCCGCGCCAGATCTCCGGCAGGCAGGAACTTTATGAAACCATCGTAGCGCTTTACTGCAGGTAA 5753MI4_002Prevotella Amino 172MSKEYFPTIGRVPFEGPESKNPLAFHYYEPDRLVLGRKMKDWLRFAMAWWHTLGQASGDQ AcidFGGQTCTYAWDEGECPVCRAKAKADAGFELMQKLGIGYFCFHDVDLVEEADTIEEYEERMRIITDYLLEKMEETGIRNLWGTANVFGHKRYMNGAATNPDFDVVARAAVQIKNAIDATIKLGGENYVFWGGREGYTSLLNTQMHREKHHLGNMLRAARDYGRAHGFKGTFLIEPKPMEPTKHQYDQDTETVIGFLRCHGLDKDFKVNIEVNHATLAGHTFEHELATAVDAGLLGSIDANRGDAQNGWDTDQFPIDNYELTLAMLQIIRNGGLAPGGSNFDAKLRRNSTDPEDIFIAHISAMDAMARALLNAAAIWTESPIQDMVRDRYASFDSGKGREFEEGRLSLEDLVAYAKEHGEPRQISGRQELYETIVALYCR 5752MI4_004 Prevotella DNA 173ATGACTAAAGAGTATTTCCCGGGAATCGGAACGATTCCGTTTGAAGGAACCAAGAGCAAGAACCCCCTGGCCTTCCATTATTATAACGCCTCCCAGGTAGTGATGGGCAAGCCCATGAAGGACTGGCTCAAGTATGCCATGGCCTGGTGGCACACCCTGGGCCAGGCCTCTGCAGACCCCTTTGGCGGCCAGACCCGCTCCTACGAATGGGACAAGGGCGAGTGCCCGTACTGCCGCGCCAAGCAGAAGGCCGATGCCGGCTTTGAGCTCATGCAGAAGCTGGGCATCGAGTACTACTGCTTCCACGACGTGGACATCATCGAGGACTGCGAGGACATTGCCGAGTACGAGGCCCGCATGAAGGACATCACGGACTACCTGCTGGAGAAGCAGAAAGAGACCGGCATCAAGAACCTCTGGGGCACCGCCAACGTGTTTGGCCACAAGCGCTACATGAACGGCGCCGCCACCAACCCTCAGTTTGACATTGTGGCCCGTGCCGCCGTCCAGATCAAGAACGCCCTGGATGCCGCCATCAAACTGGGTGGTACCAACTACGTGTTCTGGGGTGGCCGCGAAGGCTACTACACGCTGCTCAACACCCAGATGCAGCGGGAGAAGAACCACCTGGCCAAGATGCTCACCGCCGCCCGCGACTACGCCCGCGCCAAGGGCTTCAAGGGCACCTTCCTCATTGAGCCCAAACCCATGGAGCCCACCAAGCACCAGTACGACGTGGACACCGAGACCGTGATTGGTTTCATCCGCGCCAACGGCCTGGACAAGGACTTCAAGGTAAACATTGAGGTAAACCACGCCACCCTGGCCGGCCACACCTTTGAGCACGAGCTCACCGTGGCCCGCGAGAACGGCTTCCTGGGCTCCATCGACGCCAACCGCGGAGATGCCCAGAACGGCTGGGATACGGACCAGTTCCCCATCGACGCCCTGGATCTCACCCAGGCTATGATGCAGGTCATCCTCAACGGTGGCTTCGGCAATGGCGGCACCAACTTTGACGCCAAGCTCCGCCGCTCCTCCACCGATCCCGAGGACATCTTCATCGCCCACATCAGCGCCATGGATGCCATGGCACACGCCCTCCTGAACGCAGCCGCCATCCTGGAAGAGAGCCCCCTGCCCGCCATGGTCAAGGAGCGTTACGCTTCCTTCGACAGCGGTCTGGGCAAGAAGTTCGAAGAAGGCAAGGCCTCCCTGGAAGAACTTTACGAATATGCCAAGAAGAATGGAGAGCCCGTGGCCGCTTCCGGCAAACAGGAGCTCTGCGAAACTTACTTGAACCTCTATGCAAAGTAG 5752MI4_004Prevotella Amino 174MTKEYFPGIGTIPFEGTKSKNPLAFHYYNASQVVMGKPMKDWLKYAMAWWHTLGQASADP AcidFGGQTRSYEWDKGECPYCRAKQKADAGFELMQKLGIEYYCFHDVDIIEDCEDIAEYEARMKDITDYLLEKQKETGIKNLWGTANVFGHKRYMNGAATNPQFDIVARAAVQIKNALDAAIKLGGTNYVFWGGREGYYTLLNTQMQREKNHLAKMLTAARDYARAKGFKGTFLIEPKPMEPTKHQYDVDTETVIGFIRANGLDKDFKVNIEVNHATLAGHTFEHELTVARENGFLGSIDANRGDAQNGWDTDQFPIDALDLTQAMMQVILNGGFGNGGTNFDAKLRRSSTDPEDIFIAHISAMDAMAHALLNAAAILEESPLPAMVKERYASFDSGLGKKFEEGKASLEELYEYAKKNGEPVAASGKQELCETYLNLYAK 727MI4_006 Rhizobiales DNA 175GTGACTGATTTCTTCAAGGGCATCGCGCCCGTCAAGTTTGAGGGGCCGCAGAGCTCCAATCCGCTGGCCTATCGCCACTATAACAAGGACGAAATCGTCCTCGGCAAGCGGATGGAAGACCATATCCGTCCCGGCGTTGCCTATTGGCACACCTTCGCCTATGAGGGCGGCGATCCGTTTGGCGGCCGCACCTTCGATCGCCCCTGGTTCGACAAGGGTATGGACGGCGCCCGCCTCAAGGCCGACGTGGCCTTCGAACTGTTCGACCTGCTCGACGTTCCTTTCTTCTGTTTCCACGATGCTGATATCGCTCCCGAAGGCGCAACGCTGGCCGAGAGCAACCGCAATGTGCGCGAGATTGGCGAGATCTTCGCTCGCAAGATGGAAACCAGCCGCACCAAGCTGCTCTGGGGTACGGCAAACCTGTTCTCCAATCGCCGCTACATGGCCGGCGCCGCCACCAACCCGGACCCGGAAATCTTCGCCTATGCCGCTGGGCAGGTGAAGAACGTGCTGGAACTGACCCACGAACTGGGCGGCGCCAACTATGTGCTGTGGGGCGGTCGCGAGGGTTATGAAACCCTGCTCAACACCAAGATCGGCCAGGAAATGGACCAGATGGGCCGTTTTCTGTCGATGGTCGTCGAGCATGCCGAAAAGATCGGCTTCAAGGGCCAGATCCTGATCGAGCCCAAGCCGCAGGAGCCGAGCAAGCACCAGTATGACTTCGACGTTGCAACCGTTTACGGCTTCCTCAAGAAGTATGGTCTCGAAACCAAGGTGAAGTGCAATATCGAGGTCGGCCATGCCTTCCTCGCCAATCACTCCTTCGAGCATGAACTGGCTTTGGCCGCATCGCTGGGCATTCTCGGCTCGGTCGACGCCAATCGCAACGATCTACAGTCCGGCTGGGATACCGACCAGTTCCCCAATAATGTCCCCGAAACCGCACTCGCCTTCTATCAGATTCTCAAGGCGGGCGGACTGGGCAATGGCGGCTGGAACTTCGACGCCCGCGTGCGCCGCCAGTCACTTGATCCGGCCGACCTGCTGCACGGCCATATCGGCGGCCTCGACGTGCTGGCGCGCGGCCTCAAGGCCGCCGCGGCGCTGATCGAGGACGGCACCTATGACAAGGTCGTCGACGCCCGCTATGCCGGCTGGAACCAGGGCCTGGGCAAGGATATCCTTGGTGGCAAGCTGAACCTTGCCGACCTGGCTGCCAAGGTCGACGCCGAAAACCTCAACCCGCAGCCTAGGTCCGGCCAGCAGGAATATCTCGAAAACCTGATCAACCGGTTCGTTTAG 727MI4_006 RhizobialesAmino 176 MTDFFKGIAPVKFEGPQSSNPLAYRHYNKDEIVLGKRMEDHIRPGVAYWHTFAYEGGDPFAcid GGRTFDRPWFDKGMDGARLKADVAFELFDLLDVPFFCFHDADIAPEGATLAESNRNVREIGEIFARKMETSRTKLLWGTANLFSNRRYMAGAATNPDPEIFAYAAGQVKNVLELTHELGGANYVLWGGREGYETLLNTKIGQEMDQMGRELSMVVEHAEKIGFKGQILIEPKPQEPSKHQYDFDVATVYGFLKKYGLETKVKCNIEVGHAFLANHSFEHELALAASLGILGSVDANRNDLQSGWDTDQFPNNVPETALAFYQILKAGGLGNGGWNFDARVRRQSLDPADLLHGHIGGLDVLARGLKAAAALIEDGTYDKVVDARYAGWNQGLGKDILGGKLNLADLAAKVDAENLNPQPRSGQQEYLENLINRFV

5.5 Example 4 Quantification Of XI Enzyme Activity

The clones identified in the ABD and SBD screens (see Table 2) weresubcloned into vector p426PGK1 (FIG. 3), a modified version of p426GPD(ATCC accession number 87361) in which the GPD promoter was replacedwith the PGK1 promoter from Saccharomyces cerevisiae (ATCC accessionnumber 204501) gDNA. The clones were then transformed into yeast strainMYA11008.

Cells were grown as described in the materials and methods. Cell pelletswere resuspended in about 300 μl of lysis buffer: approximateconcentrations (50 mM NaH₂PO₄ (pH 8.0), 300 mM NaCl, 10 mM imidazole(Sigma, #I5513), to which was added about 2 μl/ml beta-mercaptoethanol(BME)), and protease inhibitor cocktail tablet (Roche, 11836170001) (1tablet for about 10 ml cell extract). The cell suspension was added to a2 ml screw-cap microcentrifuge tube that had been pre-aliquotted withabout 0.5 ml of acid washed glass beads (425-600 μm). Cells were lysedusing a FastPrep-24 (MP Biomedicals, Solon, Ohio) at amplitude settingof about 6 for about 3 repetitions of about 1 minute. Cells were chilledon ice for about 5 minutes between repetitions. Samples were centrifugedat about 10,000×g for about 10 minutes at 4° C. Recovered supernatantswere used in the XI enzyme activity assay. XI enzyme activity wasperformed as described in the materials and methods. Results are shownin Table 3.

TABLE 3 XI activity at pH 7.5 SEQ Volumetric ID NO: Activity FIOPC 2−60.73 2.58 4 −21.84 0.93 6 0.86 −0.05 8 −2.14 0.12 10 −2.38 0.13 12−12.82 0.54 14 −26.97 1.45 16 −76.50 4.12 18 −15.32 0.83 20 −5.33 0.2922 0.48 −0.03 24 0.36 −0.02 26 0.81 −0.04 28 −6.65 0.36 30 −9.10 0.49 32−38.10 2.05 34 −21.76 1.17 36 −13.82 0.59 38 −17.58 0.75 40 −12.34 0.5242 −74.88 3.18 44 −37.10 1.57 46 −35.57 1.51 48 −24.69 1.05 50 −32.231.37 52 −26.72 1.13 54 −90.79 3.85 56 −39.89 1.69 58 −74.26 3.15 60−11.91 0.64 62 −15.43 0.83 64 −12.98 0.70 66 −27.45 1.48 68 −29.43 1.5970 −4.54 0.24 72 −8.93 0.48 74 −0.20 0.01 76 −0.33 0.02 78 −50.55 2.1580 −57.13 2.42 82 −58.09 2.47 84 −46.42 1.97 86 −35.95 1.53 88 −2.160.09 90 −32.77 1.39 92 −30.82 1.31 94 −8.16 0.35 96 −46.18 1.96 98−30.05 1.28 100 −8.40 0.45 102 −8.34 0.45 104 −3.80 0.20 106 −4.81 0.26108 −12.06 0.65 110 −6.10 0.33 112 −7.71 0.42 114 −4.17 0.22 116 −7.070.38 118 −13.50 0.73 120 −1.15 0.06 122 0.03 0.00 124 −4.41 0.24 126−0.85 0.05 128 −14.60 0.79 130 −17.26 0.93 132 −0.75 0.04 134 −11.550.62 136 −7.20 0.39 138 0.16 −0.01 140 −3.63 0.20 142 −3.63 0.20 144−1.20 0.06 146 −16.77 0.90 148 −2.00 0.11 150 −1.40 0.08 152 −3.63 0.20154 −7.09 0.38 156 −0.96 0.05 158 −2.79 0.15 160 −3.23 0.17 162 −10.170.55 164 −0.51 0.03 166 −3.43 0.19 168 −5.65 0.30 170 −2.35 0.13 172−1.20 0.06 174 −2.29 0.12 176 −1.92 0.08 Op-XI (ABD) −23.56 NA Op-XI(SBD) −18.55 NA Vo - ctrl −1.74 NA

5.6 Example 5 Growth of Yeast Containing XI Clones on Xylose

A subset of the XI genes from Example 3 were expressed in Saccharomycescerevisiae CEN.PK2-1Ca (ATCC: MYA1108) and assayed for ability to conferthe ability to grow on xylose. This assay was carried out as follows:colonies were isolated on SC-ura+2% glucose agar plates and inoculatedinto about 3 ml “pre-cultures” of both SC-ura 2% glycerol and SC-ura 2%xylose media, incubated at about 30° C., about 220 rpm, overnight. Cellswere harvested by centrifugation (about 100×g, 5 minutes), supernatantdiscarded and washed twice and resuspended in about 1 ml of SC-ura 2%xylose. Cells were inoculated into Biolector plates, containing SC-ura,2% xylose, and inoculums were normalized to two different startingoptical densities of about OD₆₀₀ 0.2 and 0.4. Plates were covered usinggas permeable seals and incubated in a BioLector microfermentationdevice (m2p-labs, Model G-BL100) at about 30° C. for about 4 days at 800rpm and 90% humidity. Growth readings from the Biolector were acquiredfor 60-100 hours according to manufacturer's recommendations. Resultsare shown in FIG. 4.

5.7 Example 6 Ethanol Production Under Anaerobic Conditions

A subset of the XI expressing yeast clones in strain Saccharomycescerevisiae CEN.PK2-1Ca (ATCC: MYA1108) were assayed for ability toferment xylose to ethanol (EtOH). In brief, single colonies wereinoculated into about 25 ml of SC-ura medium supplemented with about0.1% glucose and about 3% xylose. Cultures were incubated undermicroaerobic conditions at about 30° C. and about 200 rpm. Samples wereharvested at about 0, 24, 48, 72 h, and ethanol concentration determinedvia HPLC standard assays. Ethanol productivity was calculated, andlisted in units of grams of EtOH per liter per hour, and FIOPC wasgenerated comparing productivity of the control Op-XI. Results are shownin Table 4.

TABLE 4 Anaerobic EtOH Production Time (h) EtOH SEQ ID NO: 0 24 48 72(g/L/h) FIOPC 6 0.28 0 0 0 −0.004 −0.5 8 0 0 0 0 0.000 0.0 10 0 0 0 00.000 0.0 14 0.37 0.28 0.71 1.24 0.013 1.7 16 0.33 0.275 0.72 1.06 0.0111.4 18 0.29 0.135 0.31 0.595 0.005 0.6 20 0.33 0 0 0 −0.004 −0.5 22 0.320 0 0 −0.004 −0.5 24 0.28 0 0 0 −0.004 −0.5 26 0.26 0 0 0 −0.003 −0.4 280.23 0.385 1.015 1.54 0.019 2.5 30 0.27 0 0 0.07 −0.003 −0.3 32 0 0.1650.48 0.815 0.012 1.5 34 0 0.125 0.33 0.615 0.009 1.1 36 0 0 0 0 0.0000.0 46 0 0.285 0.905 1.625 0.023 3.0 60 0.45 0.35 0.87 1.39 0.014 1.8 620 0 0 0.065 0.001 0.1 64 0.38 0.275 0.735 1.18 0.012 1.6 66 0 0 0.120.22 0.003 0.4 68 0 0.05 0.275 0.5 0.007 0.9 70 0 0 0 0 0.000 0.0 720.119 0 0.054 0.1685 0.001 0.1 74 0.21 0.11 0.275 0.57 0.005 0.7 76 0.280 0 0 −0.004 −0.5 90 0 0.24 0.69 1.09 0.016 2.0 100 0.104 0.642 0.1410.366 0.001 0.2 102 0.185 0 0 0.054 −0.002 −0.2 104 0.235 0.536 0 0−0.005 −0.7 106 0.188 0.4835 0 0 −0.004 −0.6 108 0.19 0.5855 0.14550.313 0.000 0.0 110 0.3 0 0 0.05 −0.003 −0.4 112 0.19 0.5535 0.1060.1135 −0.003 −0.4 114 0.174 0 0 0 −0.002 −0.3 116 0.15 0 0.0515 0.2110.001 0.1 118 0.177 0.7075 0.5065 0.941 0.009 1.1 120 0.153 0 0 0 −0.002−0.2 122 0.169 0.553 0 0.074 −0.003 −0.5 124 0.125 0 0 0 −0.002 −0.2 1260.32 0 0 0 −0.004 −0.5 128 0 0 0 0 0.000 0.0 130 0 0 0 0 0.000 0.0 1320.121 0 0 0 −0.002 −0.2 134 0.118 0 0 0.1105 0.000 0.0 136 0.108 0 0 0−0.001 −0.2 138 0.172 0.513 0 0 −0.004 −0.6 140 0.17 0.542 0 0.31350.000 −0.1 142 0.102 0 0 0 −0.001 −0.2 144 0.28 0 0 0 −0.004 −0.5 1460.103 0.635 0.263 0.563 0.004 0.5 150 0.27 0 0 0 −0.003 −0.4 149 0.27 00 0 −0.003 −0.4 152 0.17 0 0 0 −0.002 −0.3 154 0.23 0 0 0 −0.003 −0.4156 0.23 0 0 0 −0.003 −0.4 158 0.4 0 0.105 0.23 −0.002 −0.2 160 0.38 0 00 −0.005 −0.6 162 0.36 0.055 0.23 0.41 0.001 0.2 164 0.32 0 0 0 −0.004−0.5 166 0.31 0 0 0 −0.004 −0.5 168 0.32 0 0.295 0.6 0.005 0.6 170 0.1640.4995 0 0 −0.004 −0.5 172 0.27 0 0 0 −0.003 −0.4 174 0.3 0 0.17 0.3450.001 0.2 OP-XI (pos) 0.2385 0.5875 0.6965 0.81508 0.008 NA Host-(neg)0.23625 0.088125 0 0 −0.003 NA

5.8 Example 7 Impact of pH on XI Activity

Extracts from strain Saccharomyces cerevisiae CEN.PK2-1 Ca (ATCC:MYA1108, expressing XI gene candidates in vector p426PGK1, were preparedas described in the Materials and Methods and assayed for XI activity atpH 7.5 and pH 6.0. Percent activity listed was calculated by dividingthe VA at pH 6 by the VA at pH 7.5 and multiplying by 100. Results arelisted in Table 5.

TABLE 5 XI activity at pH 6 and pH 7.5 VA, VA, Percent SEQ Organism pH 6pH 7.5 activity ID NO: Classification (U/ml) (U/ml) (pH 6) 2Bacteroidales 1.92 2.59 74% 14 Bacteroides 0.32 0.98 32% 16 Bacteroides1.16 2.40 48% 32 Bacteroides 1.17 2.21 53% 38 Firmicutes 2.46 2.77 89%42 Firmicutes 1.71 2.18 79% 44 Firmicutes 0.19 0.25 76% 46 Firmicutes1.49 1.95 76% 50 Firmicutes 0.81 0.95 86% 52 Firmicutes 0.02 0.08 26% 54Neocallimastigales 1.46 2.90 51% 58 Neocallimastigales 1.89 3.05 62% 68Neocallimastigales 1.50 1.97 76% 72 Neocallimastigales 0.57 1.04 55% 78Prevotella 2.40 3.61 67% 80 Prevotella 1.52 2.29 66% 82 Prevotella 1.481.65 89% 84 Prevotella 1.79 2.96 61% 96 Prevotella 2.13 3.56 60% 116Prevotella 0.06 0.13 47% Host-neg 0.04 0.02 NA Op-XI 0.61 1.25 49%

5.9 Example 8 K_(m) for Selected XI Clones

The K_(m) and V_(max) at pH 6 were determined for a subset of the XIclones, expressed on p426PGK1 vector in Saccharomyces cerevisiaeCEN.PK2-1Ca (ATCC: MYA1108), using the XI activity assay described inthe Materials and Methods and varying the concentrations of xylose fromabout 40-600 mM. Results shown are calculated using the Hanes Plot,which rearranges the Michaelis-Menten equation(v=V_(max)[S]/(K_(m)+[S])) as: ([S]/v=K_(m)/V_(max)+[S]/V_(max)), whereplotting [S]/v against [S], resulting in a straight line and where the yintercept=K_(m)/V_(max), the slope=1/V_(max), and the xintercept=−K_(m). Results are listed in Table 6.

TABLE 6 K_(m) determination for 3 XIs SEQ ID NO: K_(m) V_(max) 78 35.227.6 96 33.7 28.0 38 28.8 28.6

5.10 Example 9 Quantification of XI Activity Expressed From SingleGenomic Integration Locus

A vector named pYDAB006 (FIG. 5A) for integration into locus YER131.5(between YER131W and YER132C) in the S. cerevisiae genome wasconstructed using conventional cloning methods. The vector backbone witha PacI site at each end was derived from pBluescript II SK (+) (AgilentTechnologies, Inc. Santa Clara, Calif.) by standard PCR techniques,which contained only the pUC origin of replication and bla gene encodingampicillin resistance protein as a selectable marker. Two 300-base pairsegments named YER131.5-A and YER 131.5-B were amplified from yeastgenomic DNA by standard PCR techniques and connected with a multiplecloning site (MCS 1:5′-GGCGCGCCTCTAGAAAGCTTACGCGTGAGCTCCCTGCAGGGATATCGGTACCGCGGCCG C-3′ (SEQID NO:181)) using the overlapping PCR technique. The PCR primers used inthe overlapping PCR are shown in Table 7 below:

TABLE 7 Primers Used in pYDAB006 Construction SEQ ID Primer NO: Sequence(Pad site is underlined) 131.5AF 182 caccattaattaaAGCTTTGTAAATATGATGAGAGAATAATATAAATCAAACG 131.5AR 183 GGCGCGCCTCTAGAAAGCTTAATCGACAAGAACACTTCTATTTATATAGGTATGAAA 131.5BF 184 GCAGGGATATCGGTACCCACCAGCGGCCGCTGAAGAAGGTTTATTTCGTTTCGCTGT 131.5BR 185caccattaattaaCCCAGGTGAGACTGGATGCTCCA TA ABMCSF 186GCCTCTAGAAAGCTTACGCGTGAGCTCCCTG CAGGGATATCGGTACCCACCAGCGGCCGC ABMCSR 187CGCTGGTGGGTACCGATATCCCTGCAGGGAG CTCACGCGTAAGCTTTCTAGAGGCGCGCC

The overlapping PCR product was then ligated with the vector backboneresulting in plasmid pYDAB006.

A vector named pYDURA01 (FIG. 5B) for generating yeast selectable andrecyclable marker was constructed using similar method as pYDAB006. TheURA3 expression cassette was amplified from yeast genomic DNA bystandard PCR techniques. The 200 base pair fingerprint sequence (namedR88: TGCGTGTGCCGCGAGTCCACGTCTACTCGCGAACCGAGTGCAGGCGGGTCTTCGGCCAGGACGGCCGTGCGTGACCCCGGCCGCCAGACGAAACGGACCGCGCTCGCCAGACGCTACCCAGCCCGTTCATGCCGGCCGCGAGCCGACCTGTCTCGGTCGCTTCGACGCACGCGCGGTCCTTTCGGGTACTCGCCTAAGAC (SEQ ID NO:188)) at both sides of URA3 cassettewas amplified by standard PCR techniques from the genomic DNA ofyBPA317, which was a diploid strain having genotypes MATa/MATalpha;URA3/ura3; YDL074.5::P(TDH3)-CBT1-T(CYC1)-R88YLR388.5::P(TDH3)-StBGL-T(CYC1)-R88/YLR388.5::P(TDH3)-StBGL-T(CYC1)-R88.The primers used in the amplification are described in Table 8 below:

TABLE 8 Primers Used in pYDURA01 Construction SEQ Sequence (KpnI andPrimer ID NO: NotI sites are underlined) NotI-KpnI-R88-F 189caatagcggccgcggtaccTGCGTGT GCCGCGAGTCCAC R88-BamHI-R 190TGTTAGGATCCGTCTTAGGCGAG TACCCGAAAGG BamHI-ura-F 191caataggatccAGGCATATTTATGGTG AAGAATAAGT ura-Xho-R 192TGTTACTCGAGAAATCATTACGAC CGAGATTCCCG XhoI-R88-F 193caatactcgagTGCGTGTGCCGCGAG TCCAC R88-NotI-R 194 TGTTAGCGGCCGCGTCTTAGGCGAGTACCCGAAAGG

An expression cassette was generated for the XI genes by cloning into avector named pYDPt005 (FIG. 5C). pYDPt005 was generated using similarmethod as pYDAB006. It contained a TDH3 promoter and a PGK1 terminatorflanking a multiple cloning site (MCS 2:5′-ACTAGTGGATCCCTCGAGGTCGACGTTTAAAC-3′ (SEQ ID NO:195), where singleunderline is SpeI site, double underline is XhoI site, and jaggedunderline is PmeI site). The promoter and the terminator were amplifiedfrom S. cerevisiae genomic DNA; an AscI site was added to the 5′ end ofthe TDH3 promoter while a KpnI site was added to the 3′ end of the PGK1terminator during amplification. Primers used in the amplification aredescribed in Table 9.

TABLE 9 Primers Used in pYDPt005 Construction Sequence (AscI and PrimerSEQ ID NO: KpnI sites are underlined) TDH-F 196 CACCAGGCGCGCCTCTAGAAAGCTTACGCGTAGTTTATCATTATCAATA CTGCCATTTCAAAGA overlap-TDH-R 197AACGTCGACCTCGAGGGATCCAC TAGTTCGAAACTAAGTTCTTGGT GTTTTAAAACToverlap-PGK-F 198 GTGGATCCCTCGAGGTCGA CGTTTAAACATTGAATTGAATTGAAATCGATAGATCAAT PGK-R 199 CACCAGCGGCCGCGGTACCGATATCCCTGCAGGGAGCTCGAAATATC GAATGGGAAAAAAAAACTGGAT

An Orpinomyces sp. XI gene (NCBI:169733248) was cloned in this vectorbetween the SpeI and XhoI sites. The Orpinomyces sp. XI expressioncassette and R88-Ura-R88 fragment were then cloned into vector pYDAB006using Asa KpnI and Nod sites; the resulting plasmid was named pYDABF006(FIG. 5D). Subsequently, the Orpinomyces sp. XI gene in pYDABF0006 wasreplaced with a subset of the XI genes of Table 2 by digestion ofpYDABF0006 with SpeI and PmeI and ligation to a DNA fragment encodingthe appropriate XI sequence which had been amplified from p426PGK1-XIconstructs. A SpeI site followed by a Kozak-like sequence (6 consecutiveadenines) was added immediately in front of the start codon of the XIgenes while a PmeI site was added to the 3′ end of the XI genes duringamplification.

XI gene integration cassettes were extracted by PacI digestion and usedto transform yeast strain yBPA130 using standard techniques.Transformants were selected for growth on SC-Ura (Synthetic Complete,Ura dropout) agar plates. Integration position and existence of XIcassette in transformants was confirmed by PCR using the primers shownin Table 10.

TABLE 10 Primers Used in Integration Verification SEQ Primer ID NO:Sequence 5′ of integration 200 ACAGGGATAACAAAGTTTCTCCAGC 3′ ofintegration 201 CATACCAAGTCATGCGTTACCAGAG 5′ of R88-ura-R88 202TTTCCCATTCGATATTTCGAGCTCC 3′ of integration 203CATACCAAGTCATGCGTTACCAGAG

Confirmed clones were then grown about 18 hours in liquid YPD to allowlooping out of the URA3 marker and were selected for growth on SC+5-FOAagar plate. The absence of the URA3 marker was confirmed by PCR.

Strains containing the confirmed XI expression cassettes were inoculatedinto about 3 ml of modified YP Media (YP+0.1% Glucose+3.0% Xylose) andincubated overnight at about 30° C. and about 220 rpm. These overnightcultures were subcultured into about 25 ml of the same media to aboutOD₆₀₀=0.2. Samples were incubated overnight at about 30° C. and about220 rpm. Cultures were harvested when OD₆₀₀ was between about 3 and 4.Pellets were collected by centrifugation for about 5 minutes at about4000 rpm. The supernatant was discarded and pellets washed with about 25ml of distilled-deionized water and centrifuged again using the sameconditions. Supernatant was discarded and the pellet frozen at about−20° C. until lysis and characterization.

Cell pellets were thawed and about 200 mg of each pellet sample wasweighed out into 2 ml microcentrifuge tubes. About 50 μl of Complete®,EDTA-free Protease Inhibitor cocktail (Roche Part#11873 580 001) at 5times the concentration stated in the manufacturer's protocol was addedto each sample. To this was added about 0.5 ml of Y-PER Plus® DialyzableYeast Protein Extraction Reagent (Thermo Scientific Part#78999) (YP+) toeach sample. Samples were incubated at about 25° C. for about 4 hours onrotating mixer. Sample supernatants were collected after centrifugationat about 10,000×g for about 10 minutes for characterization.

Total protein concentrations of the XI sample extracts prepared abovewere carried out using Bio-Rad Protein Assay Dye Reagent Concentrate(Bio-Rad, cat#500-0006, Hercules Calif.) which is a modified version ofthe Bradford method (Bradford).

Yeast physiological pH ranges are known to range from about pH 6 toabout pH 7.5 (Pena, Ramirez et al., 1995, J. Bacteriology 4:1017-1022).Ranking of XI activity at yeast physiological pH was accomplished usingthe assay conditions at pH 7.5 and modified for pH 6.0 as described inthe materials and methods. The specific activities of 20 XIs whenexpressed from a single copy integrated into the yeast YER131.5 locuswere evaluated. The results are listed in Table 11.

TABLE 11 SA of XI Expressed in an Industrial S. cerevisiae SEQ OrganismSA, pH 6 SA, pH 7.5 ID NO: Classification (U/mg) (U/mg) 2 Bacteroidales0.86 1.08 14 Bacteroides 0.33 1.07 16 Bacteroides 0.57 1.05 32Bacteroides 0.53 1.00 38 Firmicutes 1.00 0.94 42 Firmicutes 0.79 0.82 44Firmicutes 0.08 0.10 46 Firmicutes 0.62 0.69 50 Firmicutes 0.35 0.41 52Firmicutes 0.01 0.03 54 Neocallimastigales 0.64 1.17 58Neocallimastigales 0.79 1.10 68 Neocallimastigales 0.01 0.02 72Neocallimastigales 0.22 0.40 78 Prevotella 1.10 1.45 80 Prevotella 0.741.11 82 Prevotella 0.54 0.60 84 Prevotella 0.76 1.06 96 Prevotella 1.101.62 116 Prevotella 0.03 0.06 Host neg Ctrl 0.00 0.02

5.11 Example 10 Identification of Sequence Motifs in Acid Tolerant XIs

The proposed mechanism of xylose isomerases can be summarized asfollows: (i) binding of xylose to xylose isomerase, so that O3 and O4are coordinated by metal ion I; (ii) enzyme-catalyzed ring opening (theidentity of the ring-opening group remains a subject for furtherinvestigation; ring opening may be the rate limiting step in the overallisomerization process); (iii) chain extension (sugar binds in a linearextended form) in which O2 and O4 now coordinate metal ion I; (iv) O2becomes deprotonated causing a shift of metal ion II from position 1 toan adjacent position 2 in which it coordinates O1 and O2 of the sugartogether with metal ion I; (v) isomerization via an anionic transitionstate arises by a hydride shift promoted by electrophilic catalysisprovided by both metal ions; (vi) collapse of transition state by returnof metal ion II to position 1; (vii) chain contraction to apseudo-cyclic position with ligands to metal ion I changing from O2/O4back to O03/O4; (viii) enzyme-catalyzed ring closure; (ix) dissociationof xylulose from xylose isomerase (Lavie et al., 1994, Biochemistry33(18), 5469-5480).

Many XIs identified contained one or both of two signature sequencescharacteristic of XIs, [LI]EPKP.{2}P (SEQ ID NO:204) and[FL]HD[̂K]D[LIV].[PD].[GDE] (SEQ ID NO:205). Additional sequence motifspresent in the top performing Firmicutes and Prevotella XIs wereidentified. The motifs are located near the active site includingresidues in direct contact with the D-xylose and/or the metal ions. Themotifs are shown in Table 12 below:

TABLE 12 XI Sequence Motifs XI Source Motif Sequence SEQ ID NO:Firmicutes 1A P[FY][AST][MLVI][AS][WYFL]W[HT]N[LFMG]GA 206 Firmicutes 1BP[FY][AS].{2}[WYFL]W[HT][{circumflex over ( )}TV].GA 207 Firmicutes 2[GSN][IVA]R[YFHG][FYLIV]C[FW]HD.D 208 Firmicutes 3T[ASTC][NK][{circumflex over ( )}L]F. [NDH][PRKAG][RVA][FY]C 209Firmicutes 4 [WFY]D[TQVI]D.[FY][PF][{circumflex over( )}T].{2,4}[YFH]S[ATL]T 210 Firmicutes 5 GF[NH]FD[SA]KTR 211 Prevotella1A FG.QT[RK].{2}E[WYF][DNG].{2,3}[DNEGT][AT] 212 Prevotella 1BFG.QT[RK].{2}E[WYF][DNG].{3}[{circumflex over ( )}C][AP] 213 Prevotella2 [FW]HD.D[LVI].[DE]EG[{circumflex over ( )}P][TSD][IV][EA]E 214

5.12 Example 11 In Vivo Evaluation of Xylose Isomerase

Haploid S. cerevisiae strain yBPA130 (MATa::ura3) and yBPA136(MATalpha::ura3) were genetically modified to enhance C5 xyloseutilization during fermentation. The modification includes thefollowing: the native glucose repressible alcohol dehydrogenase II geneADH2 was disrupted by inserting an expression cassette of the endogenoustransaldolase gene TAL1 (SEQ ID N0:215) and xylulokinase gene XKS 1 (SEQID NO:216). PHO13 encoding the native alkaline phosphatase specific forp-nitrophenyl phosphate gene was disrupted by inserting the nativetransketolase-1 gene TKL1 (SEQ ID NO:217). Native aldose reductase geneGRE3 was disrupted by inserting native D-ribulose-5-phosphate3-epimerase gene RPE1 (SEQ ID NO:218) and Ribose-5-phosphateketol-isomerase gene RKI1 (SEQ ID NO:219). Also one expression cassetteof native galactose permease gene GAL2 (SEQ ID NO: 220) was integratedinto the S. cerevisiae strain, resulting in haploid strains pBPB007(MATa::ura3) and pBPB008 (MATalpha::ura3). The genotype of pBPB007 andpBPB008 is adh2::TAL1-XKS1, pho13::TKL1-XKS1, gre3::RPE1-RKI1 andYLR388.5::GAL2. The sequences are shown in Table 13, below:

TABLE 13 Sequence Type of SEQ ID Name sequence NO: Sequence TAL1 (S.cerevisiae) DNA 215 ATGTCTGAACCAGCTCAAAAGAAACAAAAGGTTGCTAACAACTCTCTAGAACAATTGAAAGCCTCCGGCACTGTCGTTGTTGCCGACACTGGTGATTTCGGCTCTATTGCCAAGTTTCAACCTCAAGACTCCACAACTAACCCATCATTGATCTTGGCTGCTGCCAAGCAACCAACTTACGCCAAGTTGATCGATGTTGCCGTGGAATACGGTAAGAAGCATGGTAAGACCACCGAAGAACAAGTCGAAAATGCTGTGGACAGATTGTTAGTCGAATTCGGTAAGGAGATCTTAAAGATTGTTCCAGGCAGAGTCTCCACCGAAGTTGATGCTAGATTGTCTTTTGACACTCAAGCTACCATTGAAAAGGCTAGACATATCATTAAATTGTTTGAACAAGAAGGTGTCTCCAAGGAAAGAGTCCTTATTAAAATTGCTTCCACTTGGGAAGGTATTCAAGCTGCCAAAGAATTGGAAGAAAAGGACGGTATCCACTGTAATTTGACTCTATTATTCTCCTTCGTTCAAGCAGTTGCCTGTGCCGAGGCCCAAGTTACTTTGATTTCCCCATTTGTTGGTAGAATTCTAGACTGGTACAAATCCAGCACTGGTAAAGATTACAAGGGTGAAGCCGACCCAGGTGTTATTTCCGTCAAGAAAATCTACAACTACTACAAGAAGTACGGTTACAAGACTATTGTTATGGGTGCTTCTTTCAGAAGCACTGACGAAATCAAAAACTTGGCTGGTGTTGACTATCTAACAATTTCTCCAGCTTTATTGGACAAGTTGATGAACAGTACTGAACCTTTCCCAAGAGTTTTGGACCCTGTCTCCGCTAAGAAGGAAGCCGGCGACAAGATTTCTTACATCAGCGACGAATCTAAATTCAGATTCGACTTGAATGAAGACGCTATGGCCACTGAAAAATTGTCCGAAGGTATCAGAAAATTCTCTGCCGATATTGTTACTCTATTCGACTTGATTGAA AAGAAAGTTACCGCTTAA XKS1(S. cerevisiae) DNA 216 ATGTTGTGTTCAGTAATTCAGAGACAGACAAGAGAGGTTTCCAACACAATGTCTTTAGACTCATACTATCTTGGGTTTGATCTTTCGACCCAACAACTGAAATGTCTCGCCATTAACCAGGACCTAAAAATTGTCCATTCAGAAACAGTGGAATTTGAAAAGGATCTTCCGCATTATCACACAAAGAAGGGTGTCTATATACACGGCGACACTATCGAATGTCCCGTAGCCATGTGGTTAGAGGCTCTAGATCTGGTTCTCTCGAAATATCGCGAGGCTAAATTTCCATTGAACAAAGTTATGGCCGTCTCAGGGTCCTGCCAGCAGCACGGGTCTGTCTACTGGTCCTCCCAAGCCGAATCTCTGTTAGAGCAATTGAATAAGAAACCGGAAAAAGATTTATTGCACTACGTGAGCTCTGTAGCATTTGCAAGGCAAACCGCCCCCAATTGGCAAGACCACAGTACTGCAAAGCAATGTCAAGAGTTTGAAGAGTGCATAGGTGGGCCTGAAAAAATGGCTCAATTAACAGGGTCCAGAGCCCATTTTAGATTTACTGGTCCTCAAATTCTGAAAATTGCACAATTAGAACCAGAAGCTTACGAAAAAACAAAGACCATTTCTTTAGTGTCTAATTTTTTGACTTCTATCTTAGTGGGCCATCTTGTTGAATTAGAGGAGGCAGATGCCTGTGGTATGAACCTTTATGATATACGTGAAAGAAAATTCAGTGATGAGCTACTACATCTAATTGATAGTTCTTCTAAGGATAAAACTATCAGACAAAAATTAATGAGAGCACCCATGAAAAATTTGATAGCGGGTACCATCTGTAAATATTTTATTGAGAAGTACGGTTTCAATACAAACTGCAAGGTCTCTCCCATGACTGGGGATAATTTAGCCACTATATGTTCTTTACCCCTGCGGAAGAATGACGTTCTCGTTTCCCTAGGAACAAGTACTACAGTTCTTCTGGTCACCGATAAGTATCACCCCTCTCCGAACTATCATCTTTTCATTCATCCAACTCTGCCAAACCATTATATGGGTATGATTTGTTATTGTAATGGTTCTTTGGCAAGGGAGAGGATAAGAGACGAGTTAAACAAAGAACGGGAAAATAATTATGAGAAGACTAACGATTGGACTCTTTTTAATCAAGCTGTGCTAGATGACTCAGAAAGTAGTGAAAATGAATTAGGTGTATATTTTCCTCTGGGGGAGATCGTTCCTAGCGTAAAAGCCATAAACAAAAGGGTTATCTTCAATCCAAAAACGGGTATGATTGAAAGAGAGGTGGCCAAGTTCAAAGACAAGAGGCACGATGCCAAAAATATTGTAGAATCACAGGCTTTAAGTTGCAGGGTAAGAATATCTCCCCTGCTTTCGGATTCAAACGCAAGCTCACAACAGAGACTGAACGAAGATACAATCGTGAAGTTTGATTACGATGAATCTCCGCTGCGGGACTACCTAAATAAAAGGCCAGAAAGGACTTTTTTTGTAGGTGGGGCTTCTAAAAACGATGCTATTGTGAAGAAGTTTGCTCAAGTCATTGGTGCTACAAAGGGTAATTTTAGGCTAGAAACACCAAACTCATGTGCCCTTGGTGGTTGTTATAAGGCCATGTGGTCATTGTTATATGACTCTAATAAAATTGCAGTTCCTTTTGATAAATTTCTGAATGACAATTTTCCATGGCATGTAATGGAAAGCATATCCGATGTGGATAATGAAAATTGGGATCGCTATAATTCCAAGATTGTCCCCTTAAGCGAACTG GAAAAGACTCTCATCTAA TKL1(S. cerevisiae) DNA 217 ATGACTCAATTCACTGACATTGATAAGCTAGCCGTCTCCACCATAAGAATTTTGGCTGTGGACACCGTATCCAAGGCCAACTCAGGTCACCCAGGTGCTCCATTGGGTATGGCACCAGCTGCACACGTTCTATGGAGTCAAATGCGCATGAACCCAACCAACCCAGACTGGATCAACAGAGATAGATTTGTCTTGTCTAACGGTCACGCGGTCGCTTTGTTGTATTCTATGCTACATTTGACTGGTTACGATCTGTCTATTGAAGACTTGAAACAGTTCAGACAGTTGGGTTCCAGAACACCAGGTCATCCTGAATTTGAGTTGCCAGGTGTTGAAGTTACTACCGGTCCATTAGGTCAAGGTATCTCCAACGCTGTTGGTATGGCCATGGCTCAAGCTAACCTGGCTGCCACTTACAACAAGCCGGGCTTTACCTTGTCTGACAACTACACCTATGTTTTCTTGGGTGACGGTTGTTTGCAAGAAGGTATTTCTTCAGAAGCTTCCTCCTTGGCTGGTCATTTGAAATTGGGTAACTTGATTGCCATCTACGATGACAACAAGATCACTATCGATGGTGCTACCAGTATCTCATTCGATGAAGATGTTGCTAAGAGATACGAAGCCTACGGTTGGGAAGTTTTGTACGTAGAAAATGGTAACGAAGATCTAGCCGGTATTGCCAAGGCTATTGCTCAAGCTAAGTTATCCAAGGACAAACCAACTTTGATCAAAATGACCACAACCATTGGTTACGGTTCCTTGCATGCCGGCTCTCACTCTGTGCACGGTGCCCCATTGAAAGCAGATGATGTTAAACAACTAAAGAGCAAATTCGGTTTCAACCCAGACAAGTCCTTTGTTGTTCCACAAGAAGTTTACGACCACTACCAAAAGACAATTTTAAAGCCAGGTGTCGAAGCCAACAACAAGTGGAACAAGTTGTTCAGCGAATACCAAAAGAAATTCCCAGAATTAGGTGCTGAATTGGCTAGAAGATTGAGCGGCCAACTACCCGCAAATTGGGAATCTAAGTTGCCAACTTACACCGCCAAGGACTCTGCCGTGGCCACTAGAAAATTATCAGAAACTGTTCTTGAGGATGTTTACAATCAATTGCCAGAGTTGATTGGTGGTTCTGCCGATTTAACACCTTCTAACTTGACCAGATGGAAGGAAGCCCTTGACTTCCAACCTCCTTCTTCCGGTTCAGGTAACTACTCTGGTAGATACATTAGGTACGGTATTAGAGAACACGCTATGGGTGCCATAATGAACGGTATTTCAGCTTTCGGTGCCAACTACAAACCATACGGTGGTACTTTCTTGAACTTCGTTTCTTATGCTGCTGGTGCCGTTAGATTGTCCGCTTTGTCTGGCCACCCAGTTATTTGGGTTGCTACACATGACTCTATCGGTGTCGGTGAAGATGGTCCAACACATCAACCTATTGAAACTTTAGCACACTTCAGATCCCTACCAAACATTCAAGTTTGGAGACCAGCTGATGGTAACGAAGTTTCTGCCGCCTACAAGAACTCTTTAGAATCCAAGCATACTCCAAGTATCATTGCTTTGTCCAGACAAAACTTGCCACAATTGGAAGGTAGCTCTATTGAAAGCGCTTCTAAGGGTGGTTACGTACTACAAGATGTTGCTAACCCAGATATTATTTTAGTGGCTACTGGTTCCGAAGTGTCTTTGAGTGTTGAAGCTGCTAAGACTTTGGCCGCAAAGAACATCAAGGCTCGTGTTGTTTCTCTACCAGATTTCTTCACTTTTGACAAACAACCCCTAGAATACAGACTATCAGTCTTACCAGACAACGTTCCAATCATGTCTGTTGAAGTTTTGGCTACCACATGTTGGGGCAAATACGCTCATCAATCCTTCGGTATTGACAGATTTGGTGCCTCCGGTAAGGCACCAGAAGTCTTCAAGTTCTTCGGTTTCACCCCAGAAGGTGTTGCTGAAAGAGCTCAAAAGACCATTGCATTCTATAAGGGTGACAAGCTAATTTCTCCTTTGAAAAAAGCTTTCTAA RPE1 (S. cerevisiae) DNA 218ATGGTCAAACCAATTATAGCTCCCAGTATCCTTGCTTCTGACTTCGCCAACTTGGGTTGCGAATGTCATAAGGTCATCAACGCCGGCGCAGATTGGTTACATATCGATGTCATGGACGGCCATTTTGTTCCAAACATTACTCTGGGCCAACCAATTGTTACCTCCCTACGTCGTTCTGTGCCACGCCCTGGCGATGCTAGCAACACAGAAAAGAAGCCCACTGCGTTCTTCGATTGTCACATGATGGTTGAAAATCCTGAAAAATGGGTCGACGATTTTGCTAAATGTGGTGCTGACCAATTTACGTTCCACTACGAGGCCACACAAGACCCTTTGCATTTAGTTAAGTTGATTAAGTCTAAGGGCATCAAAGCTGCATGCGCCATCAAACCTGGTACTTCTGTTGACGTTTTATTTGAACTAGCTCCTCATTTGGATATGGCTCTTGTTATGACTGTGGAACCTGGGTTTGGAGGCCAAAAATTCATGGAAGACATGATGCCAAAAGTGGAAACTTTGAGAGCCAAGTTCCCCCATTTGAATATCCAAGTCGATGGTGGTTTGGGCAAGGAGACCATCCCGAAAGCCGCCAAAGCCGGTGCCAACGTTATTGTCGCTGGTACCAGTGTTTTCACTGCAGCTGACCCGCACGATGTTATCTCCTTCATGAAAGAAGAAGTCTCGAAGGAATTGCGTTCTAGAGATTTGCTAGATTAG RKI1 (S. cerevisiae) DNA 219ATGGCTGCCGGTGTCCCAAAAATTGATGCGTTAGAATCTTTGGGCAATCCTTTGGAGGATGCCAAGAGAGCTGCAGCATACAGAGCAGTTGATGAAAATTTAAAATTTGATGATCACAAAATTATTGGAATTGGTAGTGGTAGCACAGTGGTTTATGTTGCCGAAAGAATTGGACAATATTTGCATGACCCTAAATTTTATGAAGTAGCGTCTAAATTCATTTGCATTCCAACAGGATTCCAATCAAGAAACTTGATTTTGGATAACAAGTTGCAATTAGGCTCCATTGAACAGTATCCTCGCATTGATATAGCGTTTGACGGTGCTGATGAAGTGGATGAGAATTTACAATTAATTAAAGGTGGTGGTGCTTGTCTATTTCAAGAAAAATTGGTTAGTACTAGTGCTAAAACCTTCATTGTCGTTGCTGATTCAAGAAAAAAGTCACCAAAACATTTAGGTAAGAACTGGAGGCAAGGTGTTCCCATTGAAATTGTACCTTCCTCATACGTGAGGGTCAAGAATGATCTATTAGAACAATTGCATGCTGAAAAAGTTGACATCAGACAAGGAGGTTCTGCTAAAGCAGGTCCTGTTGTAACTGACAATAATAACTTCATTATCGATGCGGATTTCGGTGAAATTTCCGATCCAAGAAAATTGCATAGAGAAATCAAACTGTTAGTGGGCGTGGTGGAAACAGGTTTATTCATCGACAACGCTTCAAAAGCCTACTTCGGTAATTCTGACGGTAGTGTTGAAGTT ACCGAAAAGTGA GAL2 (S.cerevisiae) DNA 220 ATGGCAGTTGAGGAGAACAATATGCCTGTTGTTTCACAGCAACCCCAAGCTGGTGAAGACGTGATCTCTTCACTCAGTAAAGATTCCCATTTAAGCGCACAATCTCAAAAGTATTCTAATGATGAATTGAAAGCCGGTGAGTCAGGGTCTGAAGGCTCCCAAAGTGTTCCTATAGAGATACCCAAGAAGCCCATGTCTGAATATGTTACCGTTTCCTTGCTTTGTTTGTGTGTTGCCTTCGGCGGCTTCATGTTTGGCTGGGATACCGGTACTATTTCTGGGTTTGTTGTCCAAACAGACTTTTTGAGAAGGTTTGGTATGAAACATAAGGATGGTACCCACTATTTGTCAAACGTCAGAACAGGTTTAATCGTCGCCATTTTCAATATTGGCTGTGCCTTTGGTGGTATTATACTTTCCAAAGGTGGAGATATGTATGGCCGTAAAAAGGGTCTTTCGATTGTCGTCTCGGTTTATATAGTTGGTATTATCATTCAAATTGCCTCTATCAACAAGTGGTACCAATATTTCATTGGTAGAATCATATCTGGTTTGGGTGTCGGCGGCATCGCCGTCTTATGTCCTATGTTGATCTCTGAAATTGCTCCAAAGCACTTGAGAGGCACACTAGTTTCTTGTTATCAGCTGATGATTACTGCAGGTATCTTTTTGGGCTACTGTACTAATTACGGTACAAAGAGCTATTCGAACTCAGTTCAATGGAGAGTTCCATTAGGGCTATGTTTCGCTTGGTCATTATTTATGATTGGCGCTTTGACGTTAGTTCCTGAATCCCCACGTTATTTATGTGAGGTGAATAAGGTAGAAGACGCCAAGCGTTCCATTGCTAAGTCTAACAAGGTGTCACCAGAGGATCCTGCCGTCCAGGCAGAGTTAGATCTGATCATGGCCGGTATAGAAGCTGAAAAACTGGCTGGCAATGCGTCCTGGGGGGAATTATTTTCCACCAAGACCAAAGTATTTCAACGTTTGTTGATGGGTGTGTTTGTTCAAATGTTCCAACAATTAACCGGTAACAATTATTTTTTCTACTACGGTACCGTTATTTTCAAGTCAGTTGGCCTGGATGATTCCTTTGAAACATCCATTGTCATTGGTGTAGTCAACTTTGCCTCCACTTTCTTTAGTTTGTGGACTGTCGAAAACTTGGGACATCGTAAATGTTTACTTTTGGGCGCTGCCACTATGATGGCTTGTATGGTCATCTACGCCTCTGTTGGTGTTACTAGATTATATCCTCACGGTAAAAGCCAGCCATCTTCTAAAGGTGCCGGTAACTGTATGATTGTCTTTACCTGTTTTTATATTTTCTGTTATGCCACAACCTGGGCGCCAGTTGCCTGGGTCATCACAGCAGAATCATTCCCACTGAGAGTCAAGTCGAAATGTATGGCGTTGGCCTCTGCTTCCAATTGGGTATGGGGGTTCTTGATTGCATTTTTCACCCCATTCATCACATCTGCCATTAACTTCTACTACGGTTATGTCTTCATGGGCTGTTTGGTTGCCATGTTTTTTTATGTCTTTTTCTTTGTTCCAGAAACTAAAGGCCTATCGTTAGAAGAAATTCAAGAATTATGGGAAGAAGGTGTTTTACCTTGGAAATCTGAAGGCTGGATTCCTTCATCCAGAAGAGGTAATAATTACGATTTAGAGGATTTACAACATGACGACAAACCGTGGTACAAGGCCATGCTAGAATAA

A vector named pYDAB008 rDNA (FIG. 6) for integration xylose isomeraseinto ribosomal DNA loci in S. cerevisiae genome was constructed usingconventional cloning methods. This vector can confer high copy numberintegration of genes and resulting in high-level expression of proteins.The vector was derived from pBluescript II SK (+) (Agilent Technologies,Inc., Santa Clara, Calif.). The pUC origin of replication and bla geneencoding ampicillin resistance was amplified with specific primersequences as a selectable marker for cloning. A 741 base-pair segment R1region, 253 base-pair R3 region and a 874 base-pair R2 region wereamplified from yeast genomic DNA by PCR amplifications. A multiplecloning site of SEQ ID NO:181 (:5′-GGCGCGCCTCTAGAAAGCTTACGCGTGAGCTCCCTGCAGGGATATCGGTACCGCGGCCG C-3′) wasinserted between the R1 and R3/R2 regions by assembly using overlappingPCR. All primers used in above reactions are shown in Table 14.Overlapping PCR products were then ligated in one reaction and result inrDNA integration plasmid named pYDAB008 rDNA (FIG. 6).

TABLE 14 Primers Used in pYDAB008 rDNA vector construction Sequence (PacI restriction Primer SEQ ID NO: site is underlined) Pac I-rDNA(R1)-R 221CACCATTAATTAACCCGGGGCA CCTGTCACTTTGGAA rDNA (R1)-over-R 222CGCGTAAGCTTTCTAGAGGCGC GCCAAGCTTTTACACTCTTGAC CAGCGCA AB vector-MCS-R223 CCGCTGGTGGGTACCGATATCC CTGCAGGGAGCTCACGCGTAAG CTTTCTAGAGGCGrDNA(R3)-over-R 224 CTGCAGGGATATCGGTACCCAC CAGCGGCCGCAGGCCTTGGGTGCTTGCTGGCGAA rDNA(R3)-over-R 225 ACCTCTGCATGCGAATTCTTAAGACAAATAAAATTTATAGAGAC TTGT rDNA(R2)-over-R 226 GTCTTAAGAATTCGCATGCAGAGGTAGTTTCAAGGT Pac I-rDNA(R2)-R 227 CACCATTAATTAATACGTATTTCTCGCCGAGAAAAACTT

pYDABF 0015 (comprising a nucleic acid encoding a xylose isomerase ofSEQ ID NO:78) and pYDABF-0026 (comprising a nucleic acid encoding axylose isomerase of SEQ ID NO:96) (both described in Example 10) weredigested with Asc I and Kpn I restriction enzymes (New England BiolabsInc., MA, USA) and ligated to pYDAB008 rDNA integration vector describedabove (FIG. 6). The resulting plasmids were named pYDABF-0033 (SEQ IDNO:78) and pYDABF-0036 (SEQ ID NO:96).

The rDNA integration cassette was linearized by Pac I restriction enzymedigestion (New England Biolabs Inc., MA, USA) and purified with DNAcolumn purification kit (Zymo Research, Irvine, Calif., USA). Theintegration cassette was transformed into modified haploid S. cerevisiaestrain pBPB007 (MATa::ura3) and pBPB008 (MAT alpha::ura3) using thestandard protocol described in previous examples. Transformants wereplated on SC-xylose (SC complete+2% xylose) agar plates, about 2-3 daysat about 30° C. Colonies that grew on SC-xylose agar plates were thenchecked by colony PCR analysis with primer sets shown in Table 15 (SEQID NOs:228, 229, 230 and 231) to confirm the presence of xyloseisomerase in the genome.

TABLE 15 Primers Used in Integration Verification SEQ Primer ID NO:Sequence N16PCR_F 228 CCCCATCGACAACTACGAGCTCACT N16PCR_R 229CAACTTGCCGTCCTCGAAGTCCTTG N05PCR_F 230 CGAGCCTGAGAAGGTCGTGATGGGAN05PCR_R 231 TACGTCGAAGTCGGGGTTGGTAGAA

Confirmed haploid strains were BD31328 (MATa), BD31336 (MATalpha),BD31526 (MATa) and BD31527 (MATalpha). Diploid strains BD31378(expressing a xylose isomerase of SEQ ID NO:96) and BD31365 (expressinga xylose isomerase of SEQ ID NO:78) were generated by conventional platemating on YPXylose (YP+2% xylose) agar plates, about 2 days at about 30°C. Colony PCR with specific primers checking mating types were performed(shown in Table 14) and a single colony, which has both MATa andMATalpha were picked as diploid strains BD 31378 (SEQ ID NO: 96) andBD31365 (SEQ ID NO:78).

A linear fragment encoding the URA3 sequence (SEQ ID NO:237;TTAATTAAGTTAATTACCTTTTTTGCGAGGCATATTTATGGTGAAGAATAAGTTTTGACCATCAAAGAAGGTTAATGTGGCTGTGGTTTCAGGGTCCATAAAGCTTTTCAATTCATCATTTTTTTTTTATTCTTTTTTTTGATTCCGGTTTCCTTGAAATTTTTTTGATTCGGTAATCTCCGAACAGAAGGAAGAACGAAGGAAGGAGCACAGACTTAGATTGGTATATATACGCATATGTAGTGTTGAAGAAACATGAAATTGCCCAGTATTCTTAACCCAACTGCACAGAACAAAAACCTGCAGGAAACGAAGATAAATCATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTAGCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAAAAAACTGTATTATAAGTAAATGCATGTATACTAAACTCACAAATTAGAGCTTCAATTTAATTATATCAGTTATTACCCGGGAATCTCGGTCGTAATGATTTTTATAATGACGAAAAAAAAAAAATTGGAAAGAAAAAGCTTCATGGCCTTTATAAAAAGGAACCATCCAATACCTCGCCAGAACCAAGTAACAGTATTTTACGGTTAATTAA) was transformed into BD 31378 (SEQ IDNO:96) and BD31365 (SEQ ID NO: 78) by a conventional transformationprotocol, and transformants were plated on SCXylose-URA (SyntheticComplete, Uracil dropout) for selection. Colonies were checked by PCRwith primers shown in Table 16, SEQ ID NO: 235, SEQ ID NO:236).Confirmed strains are BD31446 (SEQ ID NO: 78) and BD31448 (SEQ ID NO:96).

TABLE 16 Primers Used in Mating Type Verification SEQ Primer ID NO:Sequence 1-mating type-R 232 AGTCACATCAAGATCGTTTAT 2-mating type alpha-F233 GCACGGAATATGGGACTACTT 3-mating type a-F 234 ACTCCACTTCAAGTAAGAGTTUra fix-F 235 GAACAAAAACCTGCAGGAAACG AAGAT Ura fix-R 236GCTCTAATTTGTGAGTTTAGTA TACATGCAT

Table 17 below shows the genotypes of the resulting yeast strains:

TABLE 17 Strain Construction Name Parent Strain Description pBPB007yBPA130 MATa, ura3, adh2 :: TAL1-XKS1, pho13:: TKL1-XKS1, gre3::RPE1-RKI1 and YLR388.5:: GAL2 pBPB008 yBPA136 MATalpha, ura3, adh2 ::TAL1-XKS1, pho13:: TKL1-XKS1, gre3:: RPE1-RKI1 and YLR388.5:: GAL2BD31328 pBPB007 MATa, ura3, adh2 :: TAL1-XKS1, pho13:: TKL1-XKS1, gre3::RPE1-RKI1 and YLR388.5:: GAL2, rDNA::XI (SEQ ID NO: 96) BD31336 pBPB008MATalpha, ura3, adh2 :: TAL1-XKS1, pho13:: TKL1-XKS1, gre3:: RPE1-RKI1and YLR388.5:: GAL2, rDNA::XI (SEQ ID NO: 96) BD31526 pBPB007 MATa,ura3, adh2 :: TAL1-XKS1, pho13:: TKL1-XKS1, gre3:: RPE1-RKI1 andYLR388.5:: GAL2, rDNA::XI (SEQ ID NO: 78) BD31527 pBPB008 MATalpha,ura3, adh2 :: TAL1-XKS1, pho13:: TKL1-XKS1, gre3:: RPE1-RKI1 andYLR388.5:: GAL2, rDNA::XI (SEQ ID NO: 78) BD31378 BD31328 MATa/alpha,ura3, adh2 :: TAL1-XKS1, BD31336 pho13:: TKL1-XKS1, gre3:: RPE1-RKI1 andYLR388.5:: GAL2, rDNA::XI (SEQ ID NO: 96) BD31365 BD31526 MATa/alpha,ura3, adh2 :: TAL1-XKS1, BD31527 pho13:: TKL1-XKS1, gre3:: RPE1-RKI1 andYLR388.5:: GAL2, rDNA::XI (SEQ ID NO: 78) BD31448 BD31378 MATa/alpha,adh2 :: TAL1-XKS1, pho13:: TKL1-XKS1, gre3:: RPE1-RKI1 and YLR388.5::GAL2, rDNA::XI (SEQ ID NO: 96) BD31446 BD31365 MATa/alpha. adh2 ::TAL1-XKS1, pho13:: TKL1-XKS1, gre3:: RPE1-RKI1 and YLR388.5:: GAL2,rDNA::XI (SEQ ID NO: 78)

5.13 Example 12 Fermentation Performance of Yeast Strain ExpressingDifferent Xylose Isomerases

Fermentation performances of two different XI-expressing yeast strainswere evaluated using the DasGip fermentation systems (Eppendorf, Inc.).DasGip fermenters allowed close control over agitation, pH, andtemperature ensuring consistency of the environment during fermentation.DasGip fermenters were used to test performance of the yeast strainsexpressing the XI genes on hydrolysate (Hz) (neutralized with magnesiumbases) as a primary carbon source. Prior to the start of fermentationstrains were subjected to propagation testing consisting of two steps asdescribed below.

Seed 1:

About 1 ml of strain glycerol stock was inoculated into about 100 ml ofYP (Yeast extract, Peptone) medium containing about 2% glucose and about1% xylose in the 250 ml bellco baffled flask (Bellco, Inc.). Strainswere cultivated at about 30° C. with about 200 rpm agitation for atleast 18 hours until at full saturation. Optical density was assessed bymeasuring light absorbance at wavelength of 600 nm.

Seed 2:

About 20 ml of saturated SEED 1 (see preceding paragraph) was inoculatedinto 3 L Bioflo unit (New Brunswick, Inc.) containing about 2.1 L ofbasal medium at pH 6.0 (1% v/v inoculation). Cultivation was conductedat about 30° C. in a fed batch mode with constant air flow of about 2L/min. Agitation ramp (rpm) was about 200-626 rpm over about 15 hoursstarting at about 5 hours of elapsed fermentation time (EFT). Feedingprofile was about 0-4.8 ml/min over 20 hours. The basal medium contained(per 1 L): about 20% of neutralized hydrolysate (Hz); about 20 g/Lsucrose (from cane juice); about 35 ml of nutrients mixture (Table 18),about 1 ml of vitamin mixture (Table 19); about 0.4 ml of antifoam 1410(Dow Corning, Inc.) and water. Feed medium contained (per 1 L): about20% neutralized hydrolysate (Hz), about 110 g/L sucrose (from canejuice), about 35 ml of nutrient mixture; about 1 ml of vitamin mixture,about 0.4 ml of antifoam 1410 (Dow Corning, Inc.) and water.

TABLE 18 Nutrients mixture Component FW g/mol Conc. KH₂PO₄ H₂O 154.199.1 g/L Urea 60.06 65.6 g/L MgSO₄—7H₂O 192.4 14.6 g/L DI Water NA To1.0 L

TABLE 19 Vitamin mixture (1000x) Components mM ZnSO₄ 100 H₃BO₃ 24 KI 1.8MnSO₄ 20 CuSO₄ 10 Na₂MoO₄ 1.5 CoCl₂ 1.5 FeCl₃ 1.23

DasGip Fermentation:

Strains were tested in small scale fermentation using the DasGip systemin the industrially relevant medium containing detoxified hydrolysateand sucrose. Strains were propagated as described above; DasGipinoculation was performed using the following protocol:

Cell dry weight of SEED 2 was assessed based on the final opticaldensity. Cell dry weight and optical density (600 nm) correlation wasused to estimate the volume of the SEED 2 culture needed forfermentation. Targeted inoculation level was about 7% v/v; about 1.5 g/Lcell dry weight. Appropriate volume of SEED 2 culture was harvested bycentrifugation (about 5000 rpm for 10 min) to pellet the cells andresuspended in about 17.5 ml of PBS. Resuspended cell solution was usedto inoculate a 500 ml DasGip unit containing about 250 ml of detoxifiedhydrolysate and nutrient solution (about 3.5 ml/100 ml of medium).Fermentation was performed at about 32° C. at pH 6.3 with about 200 rpm.The duration of fermentation was about 92 hours with regular sampling.Sampling was conducted by a 25 ml steriological pipette through the portin the head plate of the DasGip unit. About 3 ml of culture were takenout, harvested by centrifugation (about 5000 rpm for 10 min) to pelletthe cells and the supernatant was submitted for analysis. Standardanalytical techniques such as high-pressure liquid chromatography (HPLC)were used to determine concentration of sugars and ethanol in themedium. Fermentation performances for yeast strains BD31378 (expressinga xylose isomerase of SEQ ID NO:96) and BD31365 (expressing a xyloseisomerase of SEQ ID NO:78) are presented in FIG. 7A and FIG. 7B,respectively.

While various specific embodiments have been illustrated and described,it will be appreciated that various changes can be made withoutdeparting from the spirit and scope of the invention(s).

1. (canceled)
 2. (canceled)
 3. (canceled)
 4. (canceled)
 5. A recombinantpolypeptide comprising an amino acid sequence having at least 95%, 97%or 98% sequence identity to amino acids 2-377 of SEQ ID NO:96.
 6. Arecombinant polypeptide comprising an amino acid sequence having atleast 80%, 85%, 90%, 93% or 95% sequence identity to amino acids 2-377of SEQ ID NO:96 and further comprises the amino acid sequence of (a) SEQID NO:212 or SEQ ID NO:213 and/or (b) SEQ ID NO:214.
 7. The recombinantpolypeptide of claim 6, which comprises an amino acid sequence having atleast 95%, 97% or 98% sequence identity to amino acids 2-377 of SEQ IDNO:96.
 8. The recombinant polypeptide of any one of claims 5 to 7, whichcomprises an amino acid sequence having at least 95%, 97% or 98%sequence identity to SEQ ID NO:96.
 9. (canceled)
 10. (canceled) 11.(canceled)
 12. (canceled)
 13. (canceled)
 14. (canceled)
 15. (canceled)16. (canceled)
 17. (canceled)
 18. (canceled)
 19. (canceled) 20.(canceled)
 21. (canceled)
 22. (canceled)
 23. (canceled)
 24. (canceled)25. (canceled)
 26. (canceled)
 27. (canceled)
 28. (canceled) 29.(canceled)
 30. (canceled)
 31. (canceled)
 32. (canceled)
 33. (canceled)34. (canceled)
 35. (canceled)
 36. (canceled)
 37. (canceled)
 38. Anucleic acid which encodes a polypeptide according to any one of claims5 to
 8. 39. A nucleic acid comprising a nucleotide sequence having atleast 70%, at least 75%, at least 80%, at least 85%, at least 90%, atleast 93%, at least 95%, at least 96%, at least 98%, or at least 99%sequence identity, or having 100% sequence identity, to the nucleotidesequence of any one of SEQ NO: 95 or to a portion thereof encoding axylose isomerase catalytic or dimerization domain.
 40. A vectorcomprising the nucleic acid of claim
 39. 41. The vector of claim 40which further comprises an origin of replication.
 42. The vector ofclaim 40 which further comprises a promoter sequence operably linked tosaid nucleotide sequence.
 43. The vector of claim 42, wherein thepromoter sequence is operable in yeast.
 44. The vector of claim 42,wherein the promoter sequence is operable in filamentous fungi.
 45. Arecombinant cell engineered to express the polypeptide of any one ofclaims 5 to
 8. 46. The recombinant cell of claim 45 which is aeukaryotic cell.
 47. The recombinant cell of claim 45 which is a yeastcell.
 48. The recombinant cell of claim 47 which is a yeast cell of thegenus Saccharomyces, Kluyveromyces, Candida, Pichia,Schizosaccharomyces, Hansenula, Klockera, Schwanniomyces, Issatehenkiaor Yarrowia.
 49. The recombinant cell of claim 48, wherein the yeastcell is of the species S. cerevisiae, S. bulderi, S. barnetti, S.exiguus, S. uvarum, S. diastaticus, K. lactis, K. marxianus or K.fragili, or Issatchenkia orientates.
 50. The recombinant cell of claim49, which is a S. cerevisiae cell.
 51. The recombinant cell of claim 50,comprising one or more genetic modifications resulting in at least one,any two, any three, any four or all of the following phenotypes: (a) anincrease in transport of xylose into the cell; (b) an increase inxylulose kinase activity; (c) an increase in aerobic growth rate onxylose; (d) an increase in flux through the pentose phosphate pathwayinto glycolysis; (e) a decrease in aldose reductase activity; (f) adecrease in sensitivity to catabolite repression; (g) an increase intolerance to ethanol, intermediates, osmolarity or organic acids; and(h) a reduced production of byproducts.
 52. The recombinant cell ofclaim 51, wherein one or more genetic modifications result in increasedexpression levels of one or more of a hexose or pentose transporter, axylulose kinase, an enzyme from the pentose phosphate pathway, aglycolytic enzyme and an ethanologenic enzyme.
 53. The recombinant cellof claim 52, wherein the increased expression levels are achieved byoverexpressing an endogenous gene or expressing a heterologous gene inthe recombinant cell.
 54. The recombinant cell of claim 51, wherein oneor more genetic modifications result in decreased expression levels ofone or more of a hexose kinase gene, the MIG1 gene, and the MIG2 genes.55. The recombinant cell of claim 51 which is engineered to express thexylose reductase (XR), xylose kinase (XK) and xylitol dehydrogenase (XD)pathway.
 56. The recombinant cell of claim 47 in which the nucleic acidis operably linked to a promoter that is insensitive to cataboliterepression.
 57. The recombinant cell of claim 56 in which the promoteris the TDH3 promoter, the PGK1 promoter, the TEF1 promoter, or the ADH1promoter.
 58. The recombinant cell of claim 45 which is a filamentousfungal cell.
 59. The recombinant cell of claim 58, wherein thefilamentous fungal cell is of the genus Aspergillus, Penicillium,Rhizopus, Chrysosporium, Myceliophthora, Trichoderma, Humicola,Acremonium or Fusarium.
 60. The recombinant cell of claim 58, whereinthe filamentous fungal cell is of the species Aspergillus niger,Aspergillus oryzae, Trichoderma reesei, Penicillium chrysogenum,Myceliophthora thermophile, or Rhizopus oryzae.
 61. A host celltransformed with the vector of any one of claim
 40. 62. The host cell ofclaim 61 which is a prokaryotic cell.
 63. The host cell of claim 62which is a bacterial cell.
 64. The host cell of claim 61 which is aeukaryotic cell.
 65. A method for producing a fermentation product,comprising culturing the recombinant cell of any one of claim 45 inmedium containing xylose under conditions in which the fermentationproduct is expressed.
 66. The method of claim 65, wherein the xylose inthe medium is provided by lignocellulosic hydrolysate.
 67. The method ofclaim 65 or claim 66, wherein the fermentation product is ethanol. 68.The method of claim 65 or claim 66, wherein the fermentation product isbutanol, diesel, lactic acid, 3-hydroxy-propionic acid, acrylic acid,acetic acid, succinic acid, citric acid, malic acid, fumaric acid,itaconic acid, an amino acid, 1,3-propane-diol, ethylene, glycerol, aβ-lactam antibiotic or a cephalosporin.
 69. The method of claim 68,wherein the recombinant cell comprises a genetic modification thatresults in decreased alcohol dehydrogenase activity.
 70. The method ofclaim 66 or claim 69, wherein the recombinant cell expresses one or moreenzymes that confers on the cell the ability to produce saidfermentation product.
 71. The method of any one of claims 65 to 70,wherein the medium further comprises glucose.
 72. The method of any oneof claims 65 to 70, wherein the recombinant cell is cultured underanaerobic conditions.
 73. The method of any one of claims 65 to 72,further comprising recovering the fermentation product.